CHEMICAL ENGINEERING JOURNAL
·
2023
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IF 15.1
Shiye Zhu,Anwei Chen,Youzheng Chai,Ruoyu Cao,Jianhua Zeng,Ma Bai,Liang Peng,Jihai Shao,Xiang Wang
As one of the widely used neonicotinoid insecticide, imidacloprid (IMI) poses serious threat on many kinds of non-target species. The acceleration of its removal from the contaminated environment is therefore an urgent task. Herein, the transformation of IMI by white-rot fungus Phanerochaete chrysosporium was examined, and the reaction mechanism underlying enzymes-mediated IMI biotransformation was elucidated. In addition, transformed products, pathways, and toxicity to Escherichia coli and Microcystis aeruginosa or the growth of rice seeds were investigated. P. chrysosporium could efficiently transform 93.5% of IMI for 6 days primarily through biotransformation, in which extracellular manganese peroxidase and lignin peroxidase substantially responded to transform IMI. IMI mainly underwent nitrate reduction , hydroxylation, and de-chlorination. A less neurologically toxic major metabolite of IMI, 6-chloronicotinic acid, was detected. Molecular dynamics simulation demonstrated that IMI stably bonded to active pocket of extracellular enzymes. This study reveals the key metabolic mechanism and fate of IMI in environment, and highlights the potential of fungi for IMI transformation.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
Infectious Medicine
·
2022
·
IF 0
Jinrong Wang,Guowei Song,Yue Ming,Jing Pan,Ruiqing Zhang,Guohao Fan,Xinxin Shen,Xuejun Ma,Lixin Li
Background Nucleic acid amplification enhancers suitable for use in a recombinase-aided amplification (RAA) assay were studied for the first time, and amplification of a long-fragment (509 bp) was initially explored. Methods Using recombinant plasmids and clinical samples, RAA fluorescence and basic methods were used to evaluate the efficacy. The fluorescence method was evaluated by threshold time and fluorescence value, and the basic method was characterized by 2% agarose gel electrophoresis. Results Taking a previously established RAA assay for HPV18 as an example, we demonstrated that the addition of 0.2 M, 0.4 M, and 0.6 M betaine and 10% pullulan could enhance the RAA. The new RAA assays with betaine and pullulan were named B-RAA and P-RAA, respectively. Using the B-RAA and P-RAA fluorescence methods, the threshold time values could be shortened by 1.72–2.32 minutes and 2.60 minutes, respectively, and the fluorescence values could be enhanced by 8847.25–9094.37 mv and 5250 mv, respectively. Using the basic method, the sensitivity could be increased 10-fold. We successfully amplified a long-fragment of 509 bp using a P-RAA assay with a sensitivity of 10 2 copies/µL (compared with 10 3 copies/µL in the RAA assay). Conclusions Thus, we concluded that betaine and pullulan are effective additives to enhance the sensitivity of RAA assays.
研究领域:
分子生物学
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生物化学
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临床医学
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DOI
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
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IF 8.5
Xingyuan Fang,Qi Zeng,Yuli Zhang,Haoyue Hou,Dingyi Yang,Kaihui Xu,Ting Guo,Hao Yuan,Tao Meng
The hydrophilic spindle-knot microfiber serves as a valuable tool for water collection to combat freshwater scarcity. However, balancing microfiber's water collection efficiency and reusability remains a challenge. To address this contradiction, a microfluidic approach utilizing enzyme-mediated, in situ biomineralization is developed to produce biomineralized rough spindle-knot calcium alginate microfibers (BSMs). Specifically, by strategically organizing enzymes within the microfiber, biomineralization occurs mostly at the knot, forming CaCO 3 particles that create a roughness contrast between the knot and joints, thereby increasing water collection efficiency. The CaCO 3 particles, grown from enzyme-mediated reactions, are firmly anchored inside the fiber, ensuring its reusability. As a result, the BSMs exhibit a water collection efficiency rate of 6.1 g·h −1 ·cm −1 , which is significantly higher than that of current microfibers. Moreover, the firmly anchored CaCO 3 can also supplement the calcium ions lost during fibers utilization, increasing the BSMs' reusability up to 108 times. Consequently, this strategy offers a novel and scalable platform for highly efficient water collection and the ability of 3D printing biomaterials with elaborate structures.
研究领域:
材料科学
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生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Zhimin Gong,Gaobo Wang,Shuai Shao,Mengjie Wang,Kun Lu,Shixiang Gao
Catalyzed oxidative reactions mediated by enzymes have been proposed as an effective remediation strategy to remove micropollutants. However, enzyme-catalyzed oxidation processes are usually limited to the substrates of phenols and amine compounds. The addition of synthetic redox mediators could extend the types of enzyme-catalyzed substrates. However, the actual applications were hindered by the high cost and potential toxicity of mediators. Here, we discovered a potential HRP-mediator system by exploring the removal of co-existing pollutants amlodipine (AML) and methylparaben (MeP). It was found that MeP served as a redox mediator could efficiently mediate the removal of AML by HRP/H 2 O 2 system. Surface electrostatic potential analysis of AML molecule suggested that MeP radicals (MeP OX ) could abstract hydrogen from the N-H site on dihydropyridine moiety of AML and then be reduced to MeP. By exploring the mediating effects of substances with MeP-like structure, Hirshfeld charge was used to evaluate the mediating efficiency of mediators. For mediating the degradation of AML, when the Hirshfeld charge of mediator radical was around − 0.3000, the mediating efficiency was the highest. This study improved the HRP-mediated system and provided an efficient and green method for the degradation of co-existing pollutants AML and MeP.
研究领域:
环境科学
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生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Libin Sun,Jingwen Li,Danying Wu,Hong Wang,Dianyu Yu
The present study aimed to investigate the application of lysophospholipids from enzymatic degumming products and different acyl donors to form structured soybean phospholipids in the lipase-mediated acidolysis reaction. Sn -1 and sn -2 fatty acid compositions of structured phospholipids from soybean phosphatidylcholine (SP-SPC) were examined, indicating that the relative content of saturated fatty acid in SP-SPC sn -2 fatty acids increased. Liquid chromatography-mass spectrometry analysis suggested that there were seven SP-SPC molecules. Spectroscopy analysis and differential scanning calorimetry thermogram analysis indicated that the structure of SP-SPC was more stable than SPC due to its unique alkyl chain conformation and phase states. Polarized light microscopy observations suggested that SP-SPC had a strong birefringence phenomenon. The preparation of liposomes with SP-SPC showed a homogeneous double-layer with an almost constant C C strength. These results have important implications for the enzymatic preparation of structured soybean phosphatidylcholine and the information on its phase states.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL DAIRY JOURNAL
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2025
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IF 3.4
Xiaoyan Feng,Donggen Zhou,Guoyang Xie,Hengyi Xu
In contaminated food, Staphylococcus aureus is a threat to human health because it can produce enterotoxins and cause various diseases, therefore, it is important to establish a fast, accurate method to detect S. aureus . In our study, recombinase aided amplification (RAA) combined with lectin-magnetic separation (LMS) was applied to detect S. aureus in skim milk and peach juice. The streptavidin-conjugated magnetic beads (MBs-SA) characterized by Fourier Transform Infrared Spectrometer was used to capture S. aureus . The performance of LMS strategy to capture S. aureus was verified by scanning electron microscope and capture efficiency was obtained in various concentrations of bacteria. The limit of detection (LOD) of this RAA-LMS assay for S. aureus was 3.6 × 10 1 CFU/mL in pure culture, 4.4 × 10 5 CFU/mL in spiked skim milk and 1.15 × 10 3 CFU/mL in spiked juice. Compared with PCR-LMS method, the LOD for RAA-LMS assay was lower, and its sensitivity was improved by three orders of magnitude. Moreover, the detection time of this RAA-LMS method was shorter than that of the standard cultural method. In conclusion, the proposed RAA-LMS assay had a great potential for the rapid and accurate detection of S. aureus .
研究领域:
微生物学
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食品安全
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分子诊断
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食品科学
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生物检测技术
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 24
·17280
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IF 5.6
Hui Li,Mingchao Wang,Kai Qu,Ruiming Xu,Haibo Zhu
It is widely recognized that macrophage cholesterol efflux mediated by the ATP-binding cassette transporter A1 (ABCA1) constitutes the initial and rate-limiting step of reverse cholesterol transport (RCT), displaying a negative correlation with the development of atherosclerosis. Although the transcriptional regulation of ABCA1 has been extensively studied in previous research, the impact of post-translational regulation on its expression remains to be elucidated. In this study, we report an AMP-activated protein kinase (AMPK) agonist called ((2R,3S,4R,5R)-3,4-dihydroxy-5-(6-((3-hydroxyphenyl) amino)-9H-purin-9-yl) tetrahydrofuran-2-yl) methyl dihydrogen phosphate (MP), which enhances ABCA1 expression through post-translational regulation rather than transcriptional regulation. By integrating the findings of multiple experiments, it is confirmed that MP directly binds to AMPK with a moderate binding affinity, subsequently triggering its allosteric activation. Further investigations conducted on macrophages unveil a novel mechanism through which MP modulates ABCA1 expression. Specifically, MP downregulates the Cav1.2 channel to obstruct the influx of extracellular Ca2+, thereby diminishing intracellular Ca2+levels, suppressing calcium-activated calpain activity, and reducing the interaction strength between calpain and ABCA1. This cascade of events culminates in the deceleration of calpain-mediated degradation of ABCA1. In conclusion, MP emerges as a potentially promising candidate compound for developing agents aimed at enhancing ABCA1 stability and boosting cellular cholesterol efflux and RCT.Keywords:ATP-binding cassette transporter A1;reverse cholesterol transport;AMP-activated protein kinase;MP;calpain-mediated degradation
研究领域:
心血管研究
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Bio Materials
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2022
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IF 0
Mengying Li,Ning Li,Junjie Qi,Dong Gao,Mei Zhou,Xiao Wei,Chengfen Xing
Mild-temperature photothermal therapy (PTT) is being extensively explored because it causes less injury to normal cells. However, the effect of mild-temperature PTT is decreased because of heat shock protein (HSP) overexpression. To solve this problem, we designed functional conjugated polymer nanoparticles (CPNs-G) that enhance the mild-temperature photothermal effect. Upon near-infrared (NIR) light irradiation, CPNs-G generate local heat to realize the photothermal effect. Meanwhile, the increased temperature enhances the catalytic activity of GOx, thus impeding the generation of adenosine triphosphate (ATP) and inhibiting HSP expression. Therefore, this work provides a strategy for overcoming thermoresistance through an enzyme-mediated starvation effect regulated by NIR light.
研究领域:
生物医学工程
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纳米技术
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癌症治疗
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光热疗法
✓ 已验证
DOI
PubMed
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2015
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IF 1.648
Zhiping Peng,Yingqi She,Lei Chen
An in situ-formed hydrogel was synthesized by enzymatic cross-linking of poly(γ-glutamic acid)–tyramine conjugates (PGA–Tyr) using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The gelation time ranged from 25 s to 5 min was accomplished by tuning the concentration of HRP, H2O2/Tyr molar ratio and the degree of substitution (DS) of Tyr groups. The storage modulus (G′), cross-link density, and mesh size can be tailored by controlling the H2O2/Tyr ratio and DS. The rheological analysis indicated that the storage modulus (G′) can be tailored from approximately 40 to over 1100 Pa with the increasing H2O2/Tyr ratio and DS. The bovine serum albumin (BSA) was used as model protein and encapsulated into the hydrogel during the enzyme-mediated cross-linking reaction. Controlled release of BSA in vitro from the PGA–Tyr hydrogel was obtained. The release rate and cumulative release amount of encapsulated BSA were manipulated by controlling the H2O2/Tyr ratio and DS. More than 90% of encapsulated BSA was released from the hydrogel with low cross-link density and lager mesh size in 60 h, while only 68% of BSA was released from the hydrogel with high cross-link density and small mesh size. The results indicated that the PGA–Tyr hydrogel is a promising material for the controlled release of therapeutic protein or peptides.
研究领域:
材料科学
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生物医学工程
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2020
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IF 3.557
Muhammad Ibrar,Houjin Zhang
Site-specific, covalent immobilization of protein is of great importance in the design of bioanalytical devices. User-defined covalent coupling of protein onto the surface has been primarily limited to a noncanonical amino acid or cysteine residues. It is desirable to develop a new approach for site-specific biofunctionalization. Herein, we demonstrate a robust and modular chemoenzymatic approach for site-specific, covalent grafting of proteins onto a surface. The synthetic strategy relies on the combination of surface amine functionalization, followed by sortase-mediated coupling. The developed method was validated by site-specific immobilization of two model proteins (glutathione S-transferase and green fluorescent protein) on cellulose and polydimethylsiloxane surfaces via a short recognition motif (LPETG). The covalent coupling of immobilized proteins at the interface was characterized by Fourier Transform Infrared Spectroscopy in attenuated total reflectance mode, X-ray photoelectron spectroscopy, atomic force microscope, and fluorescent microscopy. This enzymatic surface functionalization approach could permit an oriented, homogeneous, and site-specific covalent tethering of LPETG-tag proteins to other materials under mild conditions.
研究领域:
材料科学
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生物技术
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生物化学
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表面化学
✓ 已验证
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2024
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IF 5.8
Yanan Zhang,Yanling Song,Bijia Wang,Zhiping Mao,Xiaofeng Sui,Liduo Rong,Meixin Zhao,Xueling Feng
Controlled drug release has sparked much attention in the field of drug delivery by prolonging the duration of drug effectiveness and reducing drug toxicity. However, the development of controlled-release drug delivery systems remains a significant challenge, primarily due to inadequate drug compatibility, low biocompatibility, and low response sensitivity of the drug carriers. Herein, an innovative strategy for fabricating multifunctional organohydrogels as drug carrier systems is designed. 1-Tetradecanol/water emulsions, stabilized by cellulose acetoacetate (CAA), are initially prepared. The organohydrogels are fabricated by the ternary enzyme-mediated polymerization of the monomer - containing emulsion. The amphipathy of CAA ensures the stability of emulsions during polymerization, and CAA participates in polymerization as an initiator. Through the reversible crystallization-melting transition of 1-tetradecanol, the drug can be encapsulated in/released from the organohydrogel. Moreover, 1-tetradecanol enhances the bioavailability of water-insoluble drugs without any additional cosolvent. Therefore, biofriendly organohydrogels can serve as ideal dressings with adjustable mechanical properties, effective temperature regulation, and temperature-triggered drug release ability.
研究领域:
材料科学
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药学
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Qian Zhao,Guangyin Zhang,Dai Lu,Kejun Feng,Xingbo Shi
Herein, an ultra-sensitive aptasensor based on dual-enzyme mediated cascade- signal amplification (dubbed Demcsa aptasensor) for ampicillin (AMP) detection is proposed. The sensor’s activity involves two major steps, i.e., DNA ligase-assisted Exonuclease III (Exo III) catalyzed degradation reaction in a homogeneous solution, signal generation/amplification through the biotin-streptavidin linkage and hybridization chain reaction (HCR) on a 96-well polystyrene plate. Firstly, a detection probe was formed via the hybridization reaction between biotinylated AMP aptamer (bio-AP), HCR initiator strand (H strand) and complementary DNA (cDNA). As a result of the dual-enzyme mediated degradation, a long APH strand was formed via the DNA ligase-catalyzed ligation of bio-AP and the H strand, which served as integrated initiator to trigger the HCR reaction, thereby producing a long double-stranded DNA polymer. Subsequently, the SYBR Green I, as fluorescent dye for DNA, was added and a strong fluorescence signal was generated. In the presence of AMP, the function of DNA ligase was effectively suppressed due to the higher affinity of target-AP strand complex. As a result, the HCR reaction could not be triggered without generating a fluorescence signal, because the bio-AP cannot ligate to the H strand even when captured on the plate. The cascade- signal amplification of biotin-streptavidin linkage and the HCR reaction endowed the Demcsa aptasensor with a limit of detection (LOD) of aM, which was comparable or superior to those of the previously reported sensors. The Demcsa aptasensor has been used for the screening of AMP in food samples with favorable recoveries. The results of this work pave a new, universal way for developing efficient sensors for the detection of other trace food hazards.
研究领域:
分析化学
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生物传感器
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食品科学
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）