SOLID STATE SCIENCES
·
2024
·
IF 3.4
Ying Zhu,Tongkai Ji,Xingwei Cai,Jing Wang
A novel NiO/CeO 2 −mSiO 2 nanocages with superior peroxidase−like activities due to the synergetic effect between NiO and CeO 2 were fabricated by a simple etching−precipitation method with core−shell structured Cu 2 O@mSiO 2 as a sacrificial template. Based on the peroxidase−like activity of the NiO/CeO 2 −mSiO 2 nanocages, an enzyme cascade−triggered colorimetric sensing platform was exploited for alkaline phosphatase (ALP) detection, owing to the fact that ascorbic acid (AA) that is the ALP enzymatic hydrolysis product of ascorbic acid 2−phosphate (AAP) can efficiently reduce the oxidation product of TMB (oxTMB) generated under the catalysis of the NiO/CeO 2 −mSiO 2 . For the sensing of ALP, a good linear response was observed in the range of 0.0005–1.25 mU mL −1 with the limit of detection (LOD) as low as 0.00048 mU mL −1 . Furthermore, the proposed enzyme cascade−triggered colorimetric sensing platform could be further explored for sensing of tyrosinase, according to the fact that dopamine (DA) the catalytic product of tyramine (Tyr) by tyrosinase could initiate the redox reaction with the oxTMB acquired under the catalysis of the NiO/CeO 2 −mSiO 2 . A wide linear response was found for tyrosinase detection from 0.02 mU mL −1 to 3.4 U mL −1 with a LOD as low as 0.002 U mL −1 . This work not only offers a facile and effective approach to fabricate nanozymes with excellent catalytic activity, but also provides a promising enzyme cascade−triggered colorimetric method for the ultra−sensitive detection of biological enzymes.
研究领域:
生物化学
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纳米医学
·
分析化学
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产品关联
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 11
·117501
·
IF 3.721
Ziyin Yang,Xiao Bai,Chengcheng Qi
NiCo layered double hydroxide (NiCo-LDH) was explored as electrocatalyst for nonenzymatic detection of H2O2, in which NiCo-LDH with different morphologies were synthesized through a hydrothermal method, and then acid-etched NiCo-LDH (E-NiCo-LDH) was obtained via an acid-base reaction. The morphology, composition and structure were characterized by transmission electron microscopy and X-ray diffraction. Electrochemical measurements reveal that E-NiCo-LDH exhibits an excellent electrocatalytic activity toward H2O2 oxidation and the sensor based on E-NiCo-LDH is able to detect H2O2 in a linear range of 2.0 μM to 1.1 mM with a high sensitivity of 728.6 μA mM−1 cm−2 and a low detection limit of 0.2 μM (S/N = 3). This indicated that E-NiCo-LDH is a promising material for electrochemical sensing of H2O2.
研究领域:
材料科学
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电化学
·
分析化学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
·
IF 5.2
Chengcheng Qi,Wei Xiang,Yongping Dong,Wangbing Zhang
The development of new highly active electrocatalysts is one of the key factors to improve the performance of enzyme-free electrochemical sensors . In this paper, Co 3 V 2 O 8 was proposed as a new electrocatalyst for nonenzymatic oxidation of H 2 O 2 . A facile self-templating strategy was adopted for the synthesis of Co 3 V 2 O 8 nanomaterial which was further characterized by transmission electron microscopy (TEM), powder X-ray diffractometry (XRD), fourier translation infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and electrochemical techniques . The results showed that porous Co 3 V 2 O 8 sphere was constructed from interconnected nanoparticle with a three-dimensional porous structure. Such unique structure made the sensor based on Co 3 V 2 O 8 exhibit good electrocatalytic activity toward H 2 O 2 oxidation, and could detect H 2 O 2 with a low detection limit of 0.34 µM, a wide linear range of 2.0 µM to 6.1 mM and a high sensitivity of 541.4 µA mM −1 cm −2 . This work demonstrated an effective new sensing platform for nonenzymatic detection of H 2 O 2 .
研究领域:
材料科学
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分析化学
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电化学
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产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2018
·
IF 0
Qingqing Tan,Ruirui Zhang,Weisu Kong,Fengli Qu,Limin Lu
A novel “turn-on” fluorescent trypsin detection platform dependant on carbon dot-MnO2 (CD-MnO2) nanocomposites and ascorbic acid-loaded apoferritin (APOAA) was fabricated. The detection mechanism relied on trypsin-catalyzed enzymolysis of APOAA, which released ascorbic acid (AA) as a reducing agent to disintegrate the MnO2 nanosheets, causing the recovery of the fluorescence of CDs. An excellent performance and high sensitivity of trypsin determination were observed with a detection limit (LOD) of 0.3411 ng/mL. This work provides us with a unique strategy for trypsin detection in human serum samples, which reveals the potential applications in clinical detection.
研究领域:
生物化学
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医学诊断
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纳米材料科学
✓ 已验证
产品关联
DOI
PubMed
BIOSENSORS & BIOELECTRONICS
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2016
·
IF 7.476
Zhenfei Gao,Liqiang Zhang,Chi Ma,Qidong Zhou,Yushu Tang,Zhiqiang Tu,Wang Yang,Lishan Cui,Yongfeng Li
TiO 2 modified Co 3 O 4 acicular nanotube arrays (ANTAs) have been fabricated in this study, showing a good performance in glucose detection. In the experiment, the precursor Co(CO 3 ) 0.5 (OH)·0.11H 2 O acicular nanowire arrays (ANWAs) was first grown on the fluorine doped tin oxide (FTO) substrate by a hydrothermal method . Thereafter, the uniform pink precursor Co(CO 3 ) 0.5 (OH)·0.11H 2 O ANWAs was completely converted to the black Co 3 O 4 ANTAs thin film by alkaline treatment . After the decoration of TiO 2 , the TiO 2 /Co 3 O 4 ANTAs electrode exhibits a much higher current response to glucose compared with the Co 3 O 4 ANTAs. Importantly, this neotype composite structure of Co 3 O 4 enhances the glucose sensing performance by increasing specific surface area, additional reactive sites and synergistic effect , which make the TiO 2 /Co 3 O 4 glucose sensor show a high sensitivity of 2008.82 μA mM −1 cm −2 , a fast response time (less than 5 s) and a detection limit as low as 0.3396 μM (S/N=3). The TiO 2 /Co 3 O 4 ANTAs modified electrode exhibits a high selectivity for glucose in human serum, against ascorbic acid and uric acid .
研究领域:
材料科学
·
生物医学工程
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Wen-Jie Zhu,Da-Qian Feng,Meng Chen,Zhi-Dong Chen,Rong Zhu,Hai-Lin Fang,Wei Wang
In this contribution, we first developed a semiquantitative method for the detection of glucose with self-calibration based on bienzyme colorimetry by using tree-shaped paper strip. The GOD/HRP bienzyme system was utilized to amplify the color signal in the aqueous phase. Moreover, we employed a paper as microfluidic media for running colorimetric assay, while tree-shaped paper strip was designed to ensure uniform microfluidic flow for multiple branches. Our proposed method gives direct outcomes which can be observed by the naked eye or recorded by a simple camera. The linear range is from 1.0 × 10 −3 to 11.0 × 10 −3 M, with a detection limit of 3 × 10 −4 M. Furthermore, the effect of detection condition has been investigated and discussed comprehensively. The result of determining glucose in human serum is consistent with that of detecting standard glucose solution by using our developed approach. A low-cost, simple, and rapid colorimetric method for the simultaneous detection of glucose with self-calibration on the tree-shaped paper has been proposed.
研究领域:
分析化学
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医学诊断
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产品关联
DOI
高影响因子
Micromachines
·
2022
·Vol. 13
·期 11
·1902
·
IF 3.523
Yuke Han,Xiaomeng Bian,Misheng Liang,Tianshu Li,Lianqing Zhu,Xiaoguang Zhao,Rui You
An enzyme-free terahertz uric acid sensor based on a metallic slot array metamaterial was proposed and realized both theoretically and experimentally. The sensing model was verified in simulation and femtosecond laser processing technology was employed to ablate slots in the copper plate to fabricate metamaterials. Analytes were tested with liquid phase deposition on the metamaterial by a terahertz frequency domain spectroscopy system. Gradient concentrations of uric acid, ascorbic acid, and a mixture of them were measured separately with a good linear response. A significant decrease in sensitivity was observed in the ascorbic acid assay compared with the uric acid assay. The test results of the mixture also proved that our device is resistant to ascorbic acid. It is a simple and effective method for monitoring uric acid concentrations and the strategy of eliminating interference while modulating the resonance peak location mentioned here can be rationally projected for the development of other sensors.Keywords:uric acid detection;terahertz metamaterial;biosensor;enzyme-free detection
研究领域:
生物医学工程
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传感技术
·
光学
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产品关联
DOI
PubMed
高影响因子
COLLOID AND POLYMER SCIENCE
·
2023
·Vol. 301
·期 3
·229-237
·
IF 2.4
Yang Xiaoliang,Huo Chaofei,Ji Yuting,Xu Sheng,Zhang Cuige,Xia Chunmiao,Liu Huan,Zhang Rongli,Jin Ying,Miao Zongcheng
Thermo-responsive lysozyme/calcium alginate- g -poly-N-isopropylacrylamide nanohydrogels (Lys/CA- g -PNIPAAm NHs) were prepared from the self-assembly of Lys and sodium alginate- g -poly-N-isopropylacrylamide (SA-g-PNIPAAm) and the ionic cross-linking of CaCl 2 in aqueous solution. The Lys/CA- g -PNIPAAm NHs were immobilized on the cleaned surface of a transducer to fabricate a Lys molecularly imprinted sensor (Lys@MIP NHs sensor), which can selectively rebind to Lys in a mixture of closely related compounds. The effects of temperature on the size and the morphology of the Lys/CA- g -PNIPAAm NHs, the removal of Lys templates, the rebinding of Lys targets, and the detection performance of the Lys@MIP NHs sensor were investigated by dynamic light scattering, transmission electron microscope, and differential pulse voltammetry measurements. Compared with other Lys sensors, the fabricated Lys@MIP NHs sensor exhibited the wide linear range from 1 × 10 −9 to 1 × 10 −3 mg·mL −1 at 30 °C and showed short analysis times, high accuracy, satisfactory selectivity, stability, repeatability, reproducibility, and practical application for Lys detection in urine sample. Graphical Abstract
研究领域:
生物化学
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材料科学
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分析化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Kai Zhang,Yuwen Bao,Changjin Ou,Guoyin Zhu,Yizhou Zhang
This study presents a novel biosensor that integrates CRISPR-Cas12a technology with DNA tile-based architectures to enhance thrombin detection. Utilizing PAM-engineered toehold switches, the biosensor dynamically activates the CRISPR-Cas12a system upon thrombin recognition, significantly improving both sensitivity and specificity. The label-free electrochemical detection method offers rapid, cost-effective analysis, making it ideal for real-time clinical applications. The biosensor achieved an impressive detection limit of 103 aM, surpassing traditional methods and setting a new standard for thrombin assays. The biosensor's robust performance in complex biological samples, such as diluted human serum, demonstrates its reliability for clinical diagnostics. This is particularly important for the early diagnosis and management of thrombotic and coagulation-related disorders. By combining advanced genetic engineering with traditional electrochemical techniques, the biosensor not only optimizes detection but also expands its potential applications across various biomedical fields. Overall, this biosensor provides a powerful and innovative tool for thrombin detection, offering enhanced diagnostic accuracy, faster patient management, and potential cost savings in healthcare. Its superior performance and adaptability highlight its promise for broader adoption in clinical diagnostics and other biosensing applications.
研究领域:
分子生物学
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医学诊断
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生物传感技术
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Ruizhi Yu,Zejun Deng,P.E. Sharel,Lingcong Meng,Run Wang,Yijia Wang,Haichao Li,Xiang Wang,Kechao Zhou,Li Ma,Quiping Wei
Boron-doped diamond (BDD) microsensors feature exceptional advantages such as long-term stability, excellent antifouling properties, and ultra-low background currents, considered the ideal biosensors for non-enzymatic quantification of analytes. Herein, we reported, for the first time, the use of dual BDD electrode metallic BDD films on BDD microparticles) for enzyme-free detection of uric acid in complicated sweat environments. The BDDME with a dimension of 450 μm exhibited a fast electron transfer kinetic constant of 1.68 × 10 −2 cm s −1 towards K 3 [Fe (CN) 6 ] and K 4 [Fe (CN) 6 ] and a large electrochemically active surface area of 0.13 mm 2 , larger than the real geometric surface area. The voltammetric response currents of uric acid in sweat environments followed a good linear relation with increasing concentrations in the range of 5 μM - 500 μM, along with a detection sensitivity of 16.02 μA μM −1 cm −2 and a detection limit of 0.77 μM. In addition, the BDDME is capable of achieving highly specific quantification of uric acid in the presence of diverse interference agents such as glucose, urea, and tyrosine. In addition, the BDDME showed long-term stability with 4.6 % fluctuations in the response current during repetitive tests for 360 days and could be fully activated by rinsing with water despite the possible adsorption fouling of urine and bovine serum albumin during tests. This work demonstrated the feasibility of long-term detection of sweat uric acid using a non-enzymatic way and provides a solution for the long-term management of gout and other diseases.
研究领域:
生物传感器技术
·
医学检测
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材料科学
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产品关联
DOI
高影响因子
Journal of Ocean University of China
·
2023
·Vol. 22
·期 6
·1572-1582
·
IF 1.6
Chen Lei,Li Quan,Wang Xing,Wang Wentai,Wang Lisha
The mercury species in the ocean (MeHg, Hg 2+ ) will be enriched in marine organisms and threaten human health through the food chain. While the excessive H 2 O 2 in the metabolic process will produce hydroxyl radicals and accelerate the aging of human cells, causing a series of diseases. Hence, the cost-effective and rapid detection of mercury and H 2 O 2 is of urgent requirement and significance. Here, we synthesized emerging graphitic carbon nitride quantum dots (g-CNQDs) with high fluorescence quantum yield (FLQY) of 42.69% via a bottom-up strategy by a facile one-step hydrothermal method. The g-CNQDs can detect the H 2 O 2 and Hg 2+ through the fluorescence quenching effect between g-CNQDs and detected substances. With the presence of KI, g-CNQDs show concentration-dependent fluorescence toward H 2 O 2 , with a wide detection range of 1–1000 µmolL −1 and a low detection limit of 0.23 µmolL −1 . The g-CNQDs also show sensitivity toward Hg 2+ with a detection range of 0–0.1 µmolL −1 and a detection limit of 0.038 µmolL −1 . This dual-function detection of g-CNQDs has better practical application capability compared to other quantum dot detection. This study may provide a new strategy for g-CNQDs preparation and construct a fluorescence probe that can be used in various systems involving H 2 O 2 and Hg 2+ , providing better support for future bifunctional or multifunction studies.
研究领域:
生物化学
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环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Peng Liu,Xin Li,Xuechao Xu,Xiangheng Niu,Mengzhu Wang,Hengjia Zhu,Jianming Pan
Detection of pesticide residues is important because it is related to food and environmental safety. Although lots of sensors have been developed and applied for pesticide analysis, most of them rely on the use of vulnerable bioenzymes, weakening the highlights of these sensors. Here we recommend a bioenzyme-free colorimetric assay of malathion, a typical organophosphorus pesticide, based on the analyte inducing the oxidase-mimicking activity loss of Ag 3 PO 4 /UiO-66. Compared to counterparts, the Ag 3 PO 4 /UiO-66 composite can provide enhanced oxidase-mimetic catalytic activity to trigger the 3,3′-tetramethylbenzidine chromogenic reaction with the participation of dissolved O 2 . When malathion exists, it rapidly adsorbs onto the composite surface via their unique interactions and significantly shields the oxidase-like activity of Ag 3 PO 4 /UiO-66, thus affecting the catalytic chromogenic reaction. With such a principle, sensing of malathion in a wide linear range (0.0083–5.333 μg/mL) was realized with a detection limit 7.5 ng/mL. Given no biological elements were involved in the proposed assay, it exhibited robust and reliable performance in determining malathion in practical samples. Furthermore, a chromogenic hydrogel-based smartphone sensing system was developed for instrument-free on-site analysis of malathion. With good analytical performance and facile operation, our assay and device will find promising applications in phosphothionate pesticide determination.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）