JOURNAL OF ETHNOPHARMACOLOGY
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2014
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IF 2.939
Han-Ming Cui,Qiu-Yan Zhang,Jia-Long Wang,Jian-Long Chen,Yu-Ling Zhang,Xiao-Lin Tong
Ethnopharmacological relevance Berberine (BER) and BER-original herbal medicines have a variety of pharmacological functions and have been widely used in clinical. However, its effect of enzyme induction on cytochrome P450 (CYP) in human hepatocytes is unknown. Material and method Metabolism of berberine and its effect on main metabolic enzymes in HepG2 cell in vitro was investigated. Cocktail probe drugs, mRNA expression and protein expression were used to evaluate the metabolism potency. Meanwhile, an UPLC–MS/MS method was validated for the analysis of BER and four probe drugs in HepG2 cell. Result BER significantly increased the metabolism of midazolam, phenacetin and tolbutamide by inducing the CYP1A2 and 3A4 enzyme in a dose-dependent manner, the mRNA and protein expression of CYP1A2 and 3A4 were increased by berberine at 1000 ng·mL −1 . The activity of CYP1A2 and 3A4 could be induced by BER more than 500 ng·mL −1 in HepG2 cell, which was confirmed by the increase of its mRNA and protein expression. Conclusion BER increases the metabolism of cocktail drugs such as midazolam, phenacetin and tolbutamide by increasing the mRNA and protein expression of CYP1A2 and 3A4.
研究领域:
药理学
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生物化学
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临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
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2021
·
IF 2.882
Yun-Lai Liu,Yong Wang,Xiao-Ru He,Li-She Gan,Fan Xu,Yun-Jing Xu,Xiao-Ning Wang,Tao Shen,Zhi-Wang Zhou
Two new isoquinoline alkaloids , cryptowrayines A ( 1 ) and B ( 2 ), along with one known pavine alkaloid (-)-12-hydroxyeschscholtzidine ( 3 ), were isolated from the twigs of Cryptocarya wrayi . The structures of new compounds were elucidated by extensive spectroscopic data analysis and electronic circular dichroism (ECD) calculations. Both compounds 1 and 2 exhibited moderate quinone reductase inducing activity in Hepa 1c1c7 cells.
研究领域:
植物化学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2019
·
IF 5.256
Yuanyuan Zhu,Long Wu,Heng Yan,Zhicheng Lu,Wenmin Yin,Heyou Han
We designed a new type of MIP-SERS substrate for specific and label-free detection of patulin (PAT), by combining molecular imprinting polymer (MIP) selectivity and SERS technology sensitivity. Initially, the solid substrate of PDMS/AAO was prepared using poly dimethylsiloxane (PDMS) concreted anodized aluminum oxide (AAO) template. Then moderate Au was sputtered on the surface of PDMS/AAO to obtain Au/PDMS/AAO SERS substrate. Based on the HRP enzyme initiated in situ polymerization on the Au/PDMS/AAO, the MIP-SERS substrate was successfully synthesized with selective polymer and high tense of SERS “hot spots”. The new MIP-SERS substrate showed strong SERS enhancement effect and good selectivity for PAT. Besides, the results showed that the method owned a linear range from 5 × 10 −10 to 10 −6 M with the limit of detection (LOD) of 8.5 × 10 −11 M (S/N = 3) for PAT. The proposed method also exhibited acceptable reproducibility (relative standard deviation, RSD = 4.7%)，good stability (Raman intensity is above 80% after two weeks) and recoveries from 96.43% to 112.83% with the average RSD of 6.3%. The substrate is easy to use without complex sample pretreatment , which makes it a potential candidate as a rapid and sensitive detection method in food samples.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yiming Zhang,Yinghao Ding,Xinxin Li,Zhenghao Zhang,Xiangyang Zhang,Yumiao Chen,Zhimou Yang,Yang Shi,Zhi-Wen Hu
Alkaline phosphatase (ALP) exists in both normal and pathological tissues. Spatiotemporal variations in ALP levels can reveal its potential physiological functions and changes that occur during pathological conditions. However, it is still challenging to exploit fluorescent probes that can measure ALP activity under good spatial and temporal resolutions. Herein, enzyme-instructed self-assembly (EISA) was used to construct a high-performing analytical tool (MN-pY) to probe ALP activity. MN-pY alone (free state) showed negligible fluorescence but presented an almost 13-fold increase in fluorescence intensity in the presence of ALP (assembly state). Mechanism study indicated the increase in fluorescence intensity was due to hydrogelation and formation of supramolecular fibrils, mainly consisting of dephosphorylated MN-Y. The dephosphorylation and further fibrillation of MN-pY could induce the formation of a “hydrophobic pocket”, leading to a further increase in fluorescence intensity. Moreover, MN-pY could selectively illuminate HeLa cells with a higher ALP expression but not LO2 cells with lower ALP levels, promising a potential application in cancer diagnosis.
研究领域:
生物化学
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医学诊断
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材料科学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Veterinary Science
·
2025
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IF 2.9
Jianping Liang,Can Cui,Wenge Ren,Linglin Li,Linyi Lv,Xianhui Huang,Xiangmei Li
Introduction Tenvermectin (TVM) is a novel avermectin-class drug that has attracted attention for its superior antiparasitic potency, low toxicity, and broad-spectrum activity. However, uncertainty about its interaction with cytochrome P450 enzymes (CYPs) has raised concerns about potential therapeutic failure, increased risk of toxicity, dangerous drug combinations, and prolonged discontinuation periods. Method To address these critical safety concerns, we conducted a systematic comparative study using a highly selective and quantitatively accurate substrate conversion assay to assess and compare the effects of TVM and ivermectin (IVM) on the activities of key CYPs (CYP1A1/2, 2B1, 2C6, 2D2, and 3A1/2). Results TVM induced CYP1A, 2C, 2D and 3A activities only at high therapeutic doses (2.5 mg/kg), and its induction was significantly weaker than that of IVM at all doses tested, with the most pronounced difference for CYP3A1/2. Although TVM had weak inhibitory effects on CYP2B1 and 2D2, at therapeutic concentrations these effects are presumably unlikely to cause clinically significant CYP-mediated drug interactions. Conclusion As the first study to report the effects of TVM on CYP enzyme activity, these findings provide important experimental evidence and a theoretical framework for its clinical safety assessment, development of optimal dosing regimens, and rational polypharmacy strategies.
研究领域:
药理学
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毒理学
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药物代谢
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临床药理学
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寄生虫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
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2023
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IF 5.7
Wang Lin,Cheng Wen-Chieh,Xue Zhong-Fei,Rahman Md Mizanur,Xie Yi-Xin,Hu Wenle
Inappropriate irrigation could trigger migration of heavy metals into surrounding environments, causing their accumulation and a serious threat to human central nervous system. Traditional site remediation technologies are criticized because they are time-consuming and featured with high risk of secondary pollution. In the past few years, the microbial-induced carbonate precipitation (MICP) is considered as an alternative to traditional technologies due to its easy maneuverability. The enzyme-induced carbonate precipitate (EICP) has attracted attention because bacterial cultivation is not required prior to catalyzing urea hydrolysis. This study compared the performance of lead (Pb) and copper (Cu) remediation using MICP and EICP respectively. The effect of the degree of urea hydrolysis, mass and species of carbonate precipitation, and chemical and thermodynamic properties of carbonates on the remediation efficiency was investigated. Results indicated that ammonium ion (NH4+) concentration reduced with the increase in lead ion (Pb2+) or copper ion (Cu2+) concentration, and for a given Pb2+ or Cu2+ concentration, it was much higher under MICP than EICP. Further, the remediation efficiency against Cu2+ is approximately zero, which is way below that against Pb2+ (approximately 100%). The Cu2+ toxicity denatured and even inactivated the urease, reducing the degree of urea hydrolysis and the remediation efficiency. Moreover, the reduction in the remediation efficiency against Pb2+ and Cu2+ appeared to be due to the precipitations of cotunnite and atacamite respectively. Their chemical and thermodynamic properties were not as good as calcite, cerussite, phosgenite, and malachite. The findings shed light on the underlying mechanism affecting the remediation efficiency against Pb2+ and Cu2+.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
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2025
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IF 9.1
Yanglin Jiang,Qizheng Zhang,Xunwu Hu,Ting Liang,Ailin Sun,Chenjie Xu,Peng Wang,Ye Zhang
Understanding dynamic changes within cellular microenvironments is crucial for elucidating biological processes and developing targeted therapies. Here, we present a rapid and straightforward strategy for profiling tumor microenvironments via fluorogenic crystallization driven by enzyme-instructed excited-state intramolecular proton transfer (ESIPT). By engineering ESIPT-based fluorophores, we achieve selective crystallization with strong dual-emission ratiometric fluorescence signals that are easily visualized, offering a real-time readout of tumor microenvironmental variations. We demonstrate that this method enables the efficient detection of tumor microenvironmental features, including enzymatic activity and pH heterogeneity, across different cellular models. This approach provides a simple, efficient, and versatile tool for studying microenvironment dynamics with broad potential applications in disease diagnosis, drug discovery, and personalized medicine.
研究领域:
生物化学
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医学诊断
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药物开发
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
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2015
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IF 2.321
Li-Xia Xie,Hai-Long Wu,Yu Fang,Chao Kang,Shou-Xia Xiang,Li Zhu,Xiao-Li Yin,Hui-Wen Gu,Zhi Liu,Ru-Qin Yu
This paper presented a method for the simultaneous determination of two target analytes, tyrosine (Tyr) and levodopa (Lev), in human plasma matrices using enzyme-induced excitation-emission-kinetic third-order calibration method based on the alternating quadrilinear decomposition (AQLD) algorithm. The work skillfully exploited the oxidation reactions of Tyr and Lev using polyphenol oxidase as a catalyst. A four-way data array was constructed by the kinetic evolutions of excitation–emission matrix fluorescence for a group of samples, and followed by the four-way data decomposition. The proposed method could be applied in plasma sample analyses with satisfactory results. The actual concentration of Tyr in human plasma was identified as 8.80 ± 0.54 μg mL − 1 , but Lev was not found in the presented sample. Using high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) as reference method, the F -test and t -test suggested that there were no significant differences between these two methods. The satisfactory results indicated that this third-order calibration method has the potential to give accurate quantitative analyses, particularly in complex systems containing unknown spectral interferents and existing serious collinearity problems.
研究领域:
分析化学
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生物医学
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产品关联
DOI
高影响因子
Acta Geotechnica
·
2023
·Vol. 18
·期 10
·5299-5318
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IF 5.7
Zhao Chang,Xiao Yang,Chu Jian,Hu Ran,Liu Hanlong,He Xiang,Liu Yi,Jiang Xiang
Enzymatically induced carbonate precipitation (EICP) in heterogeneous subsurface is of interest in various geoscience and environmental applications. Through fabricating microfluidic cells with different pore networks, the pore-scale formation of biological CaCO 3 was investigated experimentally by transverse mixing using time lapse high-resolution camera. The precipitation pattern over time was processed to obtain the evolution of distribution of CaCO 3 volume fraction. The impact of flow rate and pore-scale heterogeneity were quantitatively evaluated with reaction index including overall pore filling ratio, precipitation rate and precipitation efficiency. The results showed that low flow rates and strong heterogeneity in porous media are the two favorable conditions for precipitation process due to more nucleation sites. At grain scale, a statistic of three-dimensional morphologies of individual crystals was evaluated with specific surface area and degree of anisotropy. Localized precipitates in multiple pores were adopted as a representative of the whole porous media. The analysis showed that the complex pore structures are generated due to precipitates in pores, thus limiting transport of reactants and result in the permeability reduction. We furthermore confirmed different polymorphs of calcium carbonate, mainly containing vaterite and calcite. Pore-scale analysis of biological CaCO 3 in porous media significantly contributes to the understanding of advection–diffusion-reaction coupling effect, and further revealing the role of pore network on biomineralization process.
研究领域:
地质科学
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环境科学
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生物矿化
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Bio
·
2025
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IF 10.2
Shuang Bian,Yangang Zhu,Nanxin Zhao,Yanchen Wang,Zhimou Yang,Jingjing Li
Atherosclerosis (AS) is characterized by lipid overaccumulation and progressive chronic inflammation. Macrophages over-accumulate at plaque sites and promote fibrous cap instability and plaque rupture by participating in lipoprotein uptake and metabolism, inflammatory factor secretion, and the production of enzymes and toxic metabolites. The macrophages accumulating at plaque sites are mainly derived from circulating blood-derived monocytes. Therefore, we designed a peptide 2P with an alkaline phosphatase (ALP) induced self-assembly function and P-selectin targeting function to form a supramolecular bi-antibodies 2P@VLA-4 by coassembling with VLA-4, a CD106 affinity. The dephosphorylation site and efficiency were optimized. 2P@VLA-4 displayed enhanced protein stability and binding ability to inflamed vascular endothelial cells. Through the dual-targeted blocking effect on the adhesion binding site of monocytes, 2P@VLA-4 could significantly reduce the accumulation of monocytes/macrophages at the plaque site, thereby inhibiting the progression of AS. At the same time, modification of peptides with fluorescent molecules enabled specific imaging of plaques. This study focused on the ease of design and functionalization of peptides, to synthesize supramolecular bi-antibodies compound innovatively by enzyme instructed self-assembly (EISA), achieving the significant inhibition of the adhesion and aggregation of monocytes at plaque sites. Coupling with its specific imaging capability, it may provide new approaches for the integrated design of diagnosis and treatment of AS .
研究领域:
医学
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生物化学
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纳米医学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2020
·Vol. 24
·期 3
·633-640
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IF 2.646
Liu Bingqian,Shi Weiping,Yang Yani,Cai Jie
In this paper, a simple and feasible immunoassay protocol with signal amplification for sensitive determination of alpha fetoprotein (AFP, used as a model) by using new enzyme-induced Fenton reaction (EIFR) accompanying the oxidation of o-phenylenediamine (OPD) system. We discovered that glucose oxidase (GOx) has the ability to stimulate in situ Fenton reaction coupling the oxidation of OPD. GOx-catalyze of glucose results in the formation of hydrogen peroxide (H 2 O 2 ). It can catalytically oxidize Fe 2+ to Fe 3+ , meanwhile producing hydroxyl radicals (•OH). The latter effectively initiated the catalytic oxidation of o-phenylenediamine (OPD) to 2,2-aminoazobenzene (DAP), resulting in the production of an electrochemical signal. On the basis of EIFR-OPD system, a new immunoassay protocol with GOx/anti-pAb 2 -conjugated gold nanoparticle (Ab 2 -AuNP-GOx) detection antibody can be designed for the detection of target AFP on capture antibody–functionalized transparent 96-well polystyrene microplate, monitored by recording the current peak of the generated DAP. The electrochemical current shows to be positively correlated with the concentration of target AFP. Under optimal conditions, the proposed assay exhibited good electrochemical responses for detecting target AFP in the range of 1.0 pg mL − 1 to 100 ng mL −1 with a detection limit (LOD) of 0.5 pg mL −1 (0.5 ppt) estimated at the 3 S blank level. Additionally, the precision, reproducibility, specificity, and method accuracy were also investigated with acceptable results. Importantly, the EIFR-OPD system can be further extended for the determination of other protein or biomarkers by controlling the specific recognition system (antibody or aptamer).
研究领域:
生物化学
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分析化学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
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IF 7.476
Huifang Zhang,Xiaoming Ma,Shuisheng Hu,Yue Lin,Longhua Guo,Bin Qiu,Zhenyu Lin,Guonan Chen
Development of convenient but sensitive method for influenza detection is highly important in immediate and effective clinical treatment. In this study, an ultrasensitive colorimetric approach combining the advantages of the convenience of the enzyme-induced metallization and the high specificity of enzyme-linked immunosorbent assay for the detection of influenza virus A (H7N9 as model) has been developed. Two rounds of amplification are utilized to enhance the detection sensitivity. The amplification of enzymatic reaction combines with the specific optical properties of gold nanoparticles causing the enhancing of the optical signal immensely. In addition, the increased surface area and the magnetic enrichment effect also enable the magnetic bead (MB) to catch a large number of alkaline phosphatase (ALP) and detection antibody (Ab 2 ), thus very small amounts of the virus can be easily detected. Compared with conventional method, this approach exhibits outstanding sensitivity for ALP detection, 0.2 U/L of ALP can be distinguished with a spectrometer and 2 U/L with the naked eye. And as low as 25 pg/mL of H7N9 can be detected by the naked eye. This approach shows an extensive horizon for bioassays and is available in clinical diagnosis with the advances of simplification, effectiveness, low cost and sensitive readout.
研究领域:
病毒学
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诊断医学
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纳米技术
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）