ENZYME AND MICROBIAL TECHNOLOGY
·
2021
·
IF 3.493
Zhiji Chen,Feiying Mao,Haoteng Zheng,Qinjie Xiao,Zhihao Ding,Anming Wang,Xiaolin Pei
Aromatic nitriles are important structural motifs that frequently existed in pharmaceutical drugs. Due to the convenient synthesis of aldoximes from aldehydes, the dehydration of aldoximes to corresponding nitriles by aldoxime dehydratases (Oxds) is considered as a safe and robust enzymatic production route. Although the Oxd genes are widely distributed in microbial kingdom, so far less than ten Oxds were expressed and further characterized. In this study, we found 26 predicted putative Oxd genes from the GenBank database using a genome mining strategy. The Oxd gene from Pseudomonas putida F1 was cloned and functionally expressed in Escherichia coli BL21 (DE3). The amino acid sequence of OxdF1 shows high identities of 33∼85 % to other characterized Oxds, and contained a ferrous heme as the catalytic site. The optimum reaction pH and temperature of recombinant OxdF1 were 7.0 and 35 °C, respectively. OxdF1 was stable in pH 7.0 potassium phosphate buffer at 30 °C, and its half-life was approximately 3.8 h. OxdF1 can efficiently dehydrate aromatic and heterocyclic aldoximes to nitriles, such as 2-bromobenzaldoxime, 2-chloro-6-fluorobenzaldoxime, thiophene-2-carboxaldoxime, and pyridine-3-aldoxime. Therefore, the recombinant OxdF1 shows a potential application in the cyanide-free synthesis of aromatic nitriles.
研究领域:
生物化学
·
酶学
·
药物化学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Lingzhi Zhou,Xian Xie,Xiong Tong,Jing Qi,Ruiqi Xie,Jichang Tu
This study introduced α-Benzoin oxime (BO) as a novel and efficient collector for the selective flotation of ilmenite from diopside. Micro-flotation experiments demonstrated that BO exhibits superior collection performance compared to benzohydroxamic acid (BHA) under weakly acidic to neutral conditions. At pH 8 and a BO dosage of 5× 10 -4 mol/L, flotation tests on ilmenite-diopside mixed minerals achieved a TiO 2 recovery of 89.90 % with a TiO 2 grade of 47.84 %. Contact angle measurements revealed that BO adsorption notably enhances the hydrophobicity of ilmenite surfaces. Zeta potential and FTIR analyses confirmed the selective adsorption of BO onto ilmenite. Furthermore, XPS and TOF-SIMS results indicated that coordination bonds formed between the C–O and C=N–OH groups in BO and Fe(II) ions on the ilmenite surface may facilitate BO adsorption. These findings provide valuable insights into the interaction mechanism between BO and ilmenite, highlighting its potential as an efficient collector for ilmenite flotation.
研究领域:
矿物加工工程
·
材料科学与工程
✓ 已验证
产品关联
DOI
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2025
·Vol. 226
·期 9
·2400493
·
IF 2.7
Ruotong Du,Guowen Zhou,Xiaohui Wang
It is highly desirable yet challenging to develop sustainable bio-based polyurethane with excellent mechanical properties, self-healing, and reprocessing abilities. In this study, a rigid bio-based diol (VO) containing oxime group is synthesized via the condensation reaction of lignin-derived vanillin and hydroxylamine. Subsequently, VO is introduced into a castor oil-based WPU (CWPU) system to prepare CWPU-VOs with high biobased content. The presence of dynamic oxime-carbamate bonds endowed CWPU-VOs with excellent self-healing (self-healing efficiency>95%, at 80 °C for 1 h,) and reprocessing properties. By adjusting the ratio of VO and castor oil, the mechanical properties of CWPU-VOs (tensile strength of 11.0–24.3 MPa, elongation at break of 642.2–312.0%) can be effectively tuned. In addition, due to the photolysis behavior of oxime-urethane moieties, CWPU-VOs show outstanding fluorescence anti-counterfeiting potential. This work provides a new idea for the development of high-performance waterborne polyurethane with adjustable mechanical properties, self-healing ability, and fluorescent properties.
研究领域:
材料科学
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生物基材料
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高分子化学
✓ 已验证
产品关联
DOI
高影响因子
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2021
·
IF 6.158
Xiao-Qiao Zhao,Wen-Qian Wu,Hua-bo Li,Zi-Chao Guo,Wang-Hua Chen,Li-Ping Chen
As a self-reactive substance, benzaldehyde oxime (BO) is prone to a highly exothermic runaway reaction and thermal hazard analysis and the reaction kinetics calculation of BO have great significance. In this work, the decomposition products of BO in nitrogen atmosphere were identified by GC–MS and HPLC techniques. The impact of the decomposition products on the decomposition behaviors of BO were analyzed by comparison of the ARC test results of pure BO and mixture of BO and decomposition products. It was found that N-benzylidene benzylamine was the intermediate decomposition product and benzoic acid , benzamide, N-benzyl benzamide, and 2,4,5-triphenylimidazole were the final products of BO. A two-step continuous autocatalytic reaction model was established to depict the decomposition process of BO. The kinetic parameters of the model were calculated by applying the nonlinear optimization method. Finally, thermal behaviors under different process temperature were predicted based on the kinetic model , and the time to maximum rate (TMR ad ) was predicted as 112.04 °C under 24 h, and 122.19 °C of 8 h, which offer crucial safety information to optimize the safety conditions of BO during usage, storage and transportation, which minimizes the industrial disasters.
研究领域:
化学工程
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安全科学
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DOI
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2023
·
IF 5
Pei Xiaolin,Xiao Qinjie,Feng Yumin,Chen Li,Yang Fengling,Wang Qiuyan,Li Nanxing,Wang Anming
Abstract Nitriles are of significant interest in the flavor and fragrance industries with potential application in cosmetics due to their higher stability than analogous aldehydes. However, the traditional methods to prepare nitriles need toxic reagents and hash conditions. This work aimed to develop a chemoenzymatic strategy to synthesize nitriles from natural aldehydes with aldoxime as the intermediate. A non-classical aldoxime dehydratase (Oxd) was discovered from the fungus Aspergillus ibericus (OxdAsp) to catalyze the dehydration of aldoximes to corresponding nitriles under mild conditions. The amino acid sequence of OxdAsp exhibits an approximately 20% identity with bacterial Oxds. OxdAsp contains a heme prosthetic group bound with the axial H287 in the catalytic pocket. The structure models of OxdAsp with substrates suggest that its catalytic triad is Y138-R141-E192, which is different from the classically bacterial Oxds of His-Arg-Ser/Thr. The catalytic mechanism of OxdAsp was proposed based on the mutagenesis of key residues. The hydroxyl group of the substrate is fixed by E192 to increase its basicity. Y138 acts as a general acid-based catalyst, and its phenolic proton is polarized by the adjacent R141. The protonated Y138 would donate a proton to the hydroxyl group of the substrate and eliminate a water molecule from aldoxime to produce nitrile. The recombinant OxdAsp can efficiently dehydrate citronellal oxime and cinnamaldoxime to citronellyl nitrile and cinnamonitrile in aqueous media, which are applied as fragrance ingredients in the food and cosmetic fields. Graphical abstract
Key points • A novel aldoxime dehydratase from the Aspergillus genus was first characterized as a heme-binding protein. • The catalytic mechanism was predicted based on the molecular interactions of the catalytic pocket with the substrate. • A chemoenzymatic strategy was developed to synthesize nitriles from natural aldehydes with aldoxime as the intermediate.
研究领域:
生物化学
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生物催化
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有机化学
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香精香料行业
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酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Jie Zheng,Anruo Luo,Yan Jin,Baicheng Feng,Jianhua Chen,Xingrong Zhang
In this study, 5-nonyl salicylaldoxime (5-NSA) was synthesized and used as a malachite collector. Its flotation performances and adsorption mechanism were systematically investigated by micro-flotation experiments, contact angle measurements, SEM-EDS, FT-IR, XPS and DFTB+ simulations. The micro-flotation test results showed that 5-NSA produced high recovery of malachite at pH 9.0–10.0, while showing lower collecting power to calcite or quartz. The contact angle analysis results indicated that the hydrophobicity of malachite surface was promoted after the adsorption of 5-NSA. SEM confirmed the adsorption of 5-NSA on malachite surface. FT-IR and XPS analysis results confirmed that O and N atoms in the oxime group were immobilized on malachite surface with Cu through O Cu O and N Cu O bonding. Their adsorption performances on malachite surface were simulated by DFTB+ method, including 5-NSA, tert -butylsalicylaldehyde oxime (TBSA) and salicylaldoxime agents, which analyzed the bond lengths of their covalent bond formation with Cu and speculated their adsorption properties. In general, according to the experimental test and simulation results, 5-NSA showed a stronger power to malachite than TBSA and salicylaldoxime, and only a small dosage can produce a high malachite recovery. The findings of this research will be helpful to improve the comprehensive utilization efficiency of malachite and promote the metallurgy of copper oxide ores.
研究领域:
矿物加工工程
·
材料科学与工程
✓ 已验证
DOI
高影响因子
Journal of Central South University
·
2018
·Vol. 25
·期 4
·701-708
·
IF 0.761
Kong Qian,Bai Xiu-zhi,Lin Hui-ju,Li Hai-pu,Yang Zhao-guang
For the determination of salicylaldoxime in environmental water samples, a stable and rapid method with low detection was proposed and established, based on the liquid-liquid extraction-high performance liquid chromatography with ultraviolet detector. Parameters including extraction solvent, ionic strength, solution pH, and extraction pattern were discussed for the optimal quantification of salicylaldoxime-spiked water. When the described method was applied to four spiked water samples, the obtained average extraction recovery rate was found between 87%–107% and relative standard deviation was below 6%. At the same time, good linear relationships were observed for spiked water samples from 0.01 to 10 μg/mL ( R 2 =0.9993). In addition, the detection limit of salicylaldoxime was revealed between 0.003–0.008 μg/mL, which is two orders of magnitude lower than previously reported results. Thus, the presented method may be advantageous for the high-efficiency determination of salicylaldoxime in water samples.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Tingnan Xiao,Yating Wang,Yongshen Hao,Zidong Cai,Meiying Song,Jincan He,Zhengquan Su,Yan Bai
In weakly acidic BR buffer solution, a convenient and sensitive resonance Rayleigh scattering (RRS) method for the detection of Cu(Ⅱ) was developed based on the formation of salicylaloxime-Cu(Ⅱ) supramolecular complex by complexation reaction. The coordination reaction of salicylaldehyde oxime with Cu(Ⅱ) led to the significant enhancement of the resonance Rayleigh scattering intensity of the solution, and the increase of the resonance Rayleigh scattering intensity was proportional to the concentration of Cu (Ⅱ). When organic compounds with different molecular structures were introduced into the system, the resonant Rayleigh scattering intensity of the solution system changed differently. The interaction between the compounds and the spatial structure of the complexes were characterized by transmission electron microscopy (TEM), Fourier real-time infrared spectroscopy (FT-IR), zeta potential and particle size. The results showed that when 2-methylimidazole was introduced, the layered texture of Cu(Ⅱ)-CH 3 COO - -H 2 PO 4 - -salicylaldoxime system changed to porous reticular spatial structure, and the RRS value increased significantly. Under the optimal conditions, in the range extending from 0.08 to 2.81 µmol/L, the equation of linear regression was Δ I = 3202 c + 10.88 ( c , µmol/L) with a correlation coefficient 0.9996, and the detection limit was estimated to be 4.23 × 10 -2 µmol/L. The method was successfully applied for the determination of Cu(Ⅱ) in tap and river water sample, suggesting its great potential toward Cu(Ⅱ) analysis.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
Polymer Chemistry
·
2015
·Vol. 6
·期 21
·4020-4029
·
IF 5.52
Jianbing Huang,Lvhuan Lin,Hui Liang,Jiang Lu
A facile synthetic route to branched graft copolymers has been developed by combination of self-condensing vinyl polymerization (SCVP), reversible addition–fragmentation chain transfer (RAFT) polymerization and aldehyde–aminooxy reaction. RAFT polymerization of 2-(dimethylamino)ethyl methacrylate (DEM) in the presence of a newly designed aldehyde-containing chain-transfer monomer, 6-(2-formyl-4-vinylphenoxy)hexyl-2-(dodecylthiocarbonothioylthio)-2-methylpropanate (FHDM), led to branched polyDEM (BPDEM) bearing aldehyde functionalities on its branching points. The degree of branching and the average branch length of the resulting BPDEM can be readily tuned by manipulation of the DEM/FHDM feed ratio. The aldehyde groups on the branching points of BPDEM were then reacted with aminooxy-terminated poly(ethylene oxide)s (PEO-ONH2), affording the structurally well-defined branched graft copolymer BPDEM-g-PEO with a branched polyDEM backbone and PEO grafted chains. The thermo-induced micellization behavior of BPDEM-g-PEO in water was investigated. Opportunities are open for BPDEM-g-PEO to form either multimolecular micelles or unimolecular micelles via simply adjusting the chain length of grafted PEO. Further modification of BPDEM-g-PEO by quaternization resulted in branched cationic polyelectrolytes which are capable of capturing negatively charged guest molecules via electrostatic complexation to form in situ self-assembled nanoparticles with simultaneous encapsulation of the guests.
研究领域:
高分子化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL RESEARCH
·
2018
·
IF 0.668
Xiaoyun Ma,Daqin He,Zhengjian Chen
A simple method for the preparation of nitriles in moderate to good yield has been achieved by treatment of aromatic and heterocyclic aldoximes with malononitrile in water at reflux in the presence of copper acetate as catalyst. Arylaldoximes with an electron-donating group showed the highest reactivity, their conversion being achievable at room temperature.
研究领域:
有机化学
·
合成化学
✓ 已验证
DOI
高影响因子
ORGANIC ELECTRONICS
·
2023
·
IF 3.2
Wenjin Zeng,Chaochao Yuan,Yuting Shi,Enzhi Li,Wei Hua,Qiaoli Niu,Kasim Ocakoglu,Ruidong Xia,Yong Min
The process of the antisolvent rinsing plays an important role during the formation of the perovskite crystals. Since the function of the single-component antisolvent may be limited, a reducing agent named acetaldoxime (AO) was applied as the additive for the antisolvent. The analysis of both the surface morphology and the crystal structure indicates that AO can effectively improve the quality of the FASnI 3 perovskite film. The defects of the perovskite film can be greatly suppressed. Moreover, due to the increase of the crystal grain size with the surface roughness reducing at the same time, the charge recombination resistance of the FASnI 3 perovskite film increases. Therefore, the devices modified by AO have higher short-circuit current, fill factor as well as power conversion efficiency. At the same time, the surface of the FASnI 3 perovskite film becomes more hydrophobic, which benefits impeding the permeation of the ambient vapor into the perovskite film. The results of lifetime testing show that the stability of the devices is greatly improved.
研究领域:
材料科学
·
能源科学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 9
·6910-6917
·
IF 3.201
Chao Xu,Ying Qian,Zheng-qing Qi,Chang-gui Lu,Yi-ping Cui
A conjugated BODIPY–triphenylamine multi-aldoxime (BTN) was designed and synthesized by the Sonogashira coupling reaction. The chemodosimeter showed a red emission (670 nm), fast response (within seconds), high selectivity and good sensitivity towards hypochlorite based on the redox reaction of four aldoximes. BTN possessed two original absorption peaks at 366 nm and 588 nm, respectively, corresponding to fluorescence emissions at 440 nm and 660 nm, which exhibited a ratiometric change in the presence of hypochlorite. With the addition of hypochlorite, the redox reaction of aldoxime led to the termination of isomerization of the CN bond, the fluorescence intensity at 660 nm gradually changed from weak to strong, accompanied by a slight 10 nm red-shift; in contrast, the fluorescence intensity began to decrease at 440 nm. Moreover, as the two-photon excited fluorescent NaOCl probe, BTN afforded an enhanced signal response for hypochlorite at 686 nm under two-photon excitation (λTPEFex = 800 nm). Meanwhile, successful imaging of living cells was carried out for BTN towards hypochlorite in A-549 cells. The results demonstrated that BTN had potential application prospects and was promising for practical application in biological systems and environmental systems.
研究领域:
化学
·
生物分析
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）