Optical Materials Express
·
2020
·Vol. 11
·期 1
·171-179
·
IF 3.064
Shi-Tong Xu,Shi-Tong Xu,Fei Fan,Shu Chen,Yanzhao Xing,Yuebo Gao,Huixin Li,Guangcheng Niu,Sheng-Jiang Chang,Sheng-Jiang Chang,Sheng-Jiang Chang
In this work, a new strategy was proposed for active control of mm-thick liquid crystals (LC) cell to realize the polarization manipulation in terahertz (THz) regime, which through the electric field control and static magnetic field pre-anchoring. The LC cell was fabricated by a nematic 5CB LC and two silica substrates that were coated with the graphite layer as the transparent electrode. Under the pre-anchoring of the static magnetic field, the optical axis of LC can be precisely controlled by the variable electric field. By using a THz-TDS with a wire grid polarizer, the output THz polarization from the LC cell can be deduced from the amplitude and phase shift of ±45° components. Here, we systematically analyzed three different outfield configurations. Only if the ±45° components that output from the polarizer have phase shifts, can the polarization state conversion be realized. The results show that the linear to circular or the cross-polarization conversion were realized under the specific electric field. This work provides a new approach for the thick-LC layer anchoring and orientation control, and also the tunable polarization manipulation of THz LC devices.
研究领域:
光学
·
材料科学
✓ 已验证
DOI
高影响因子
NANO
·
2022
·
IF 1.438
Junfeng Zhang,Yajie Li,Yuxi Zhang,Yinghua Wu,Jiale Ju,Wen He,Chenchen Li
Magnetic nanoparticles (MNPs) used in bio-application have accelerated the development of “theranostics” which contains imaging, therapy and drug delivery. However, in a protein-rich physiological fluid, MNPs are ineluctable to a contact with protein to form “protein corona”, which will significantly change the physicochemical properties of the particles and their biological behavior. Therefore, we investigated the influence of protein and static magnetic field (SMF) on the cytotoxicity and cellular uptake of MNPs. The Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) were synthesized by solvothermal method with a mean diameter of ∼ 1 0 0 nm functionalized with carboxyl groups. After a period of 12 h incubation in complete medium (CM), the wrapping of the Fe 3 O 4 NPs by protein adsorption resulted in about 40-nm increase of the hydrodynamic size. Their stability in CM and water solution did not broaden significantly over time, which is exactly opposite in serum-free media (SFM). When transferred to SFM with high ionic-strength, the Fe 3 O 4 NPs aggregated and settled quickly. We explore that the cell viability was decreased in proportion to the concentration and incubating time of Fe 3 O 4 NPs, the absence of FBS and the presence of SMF exposure, respectively. Quantitative analysis showed that cells could incorporate more NPs in SFM, about five-fold more than in CM. In addition, it is obvious that exposure to SMF resulted in more cellular uptake both in CM and SFM. Our results demonstrated that Fe 3 O 4 NPs in SFM and SMF exposure would settle down to cell rapidly, which caused more cellular uptake and higher cytotoxicity.
研究领域:
生物医学工程
·
毒理学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2016
·Vol. 8
·期 14
·7544-7555
·
IF 7.76
Ziyao Liu,Xiaohui Zhan,Minggang Yang,Qi Yang,Xianghui Xu,Fang Lan,Yao Wu,Zhongwei Gu
In recent years, it is becoming increasingly evident that once nanoparticles come into contact with biological fluids, a protein corona surely forms and critically affects the biological behaviors of nanoparticles. Herein, we investigate whether the formation of protein corona on the surface of superparamagnetic iron oxides (SPIOs) is influenced by static magnetic field. Under static magnetic field, there is no obvious variation in the total amount of protein adsorption, but the proportion of adsorbed proteins significantly changes. Noticeably, certain proteins including apolipoproteins, complement system proteins and acute phase proteins, increase in the protein corona of SPIOs in the magnetic field. More importantly, the magnetic-dependent protein corona of SPIOs enhances the cellular uptake of SPIOs into the normal cell line (3T3 cells) and tumor cell line (HepG2 cells), due to increased adsorption of apolipoprotein. In addition, SPIOs with the magnetic-dependent protein corona cause high cytotoxicity to 3T3 cells and HepG2 cells. This work discloses that superparamagnetism as a key feature of SPIOs affects the composition of protein corona to a large extent, which further alters the biological behaviors of SPIOs.
研究领域:
纳米技术
·
生物材料
·
生物物理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER COMPOSITES
·
2024
·
IF 4.8
Jiaxing Guo,Qin Zhou,Jikai Xie,Hong Yin,Zhi Hao,Zhu Luo,Le Yang,Cheng Zhang
Graphene-loaded triiron tetraoxide/silicone-rubber composites containing highly loaded graphene-loaded Fe 3 O 4 (Fe 3 O 4 @RGO) particles were fabricated using static magnetic field-induced alignment. The Fe 3 O 4 @RGO particles were characterized through Fourier transform infrared, x-ray diffraction, SEM, and XPS analyses. Subsequently, the influence of added material and the strength of the magnetic field on the alignment distribution of the filler were examined through a synergistic combination of SEM and Raman spectroscopy. The electrical conductivity and electromagnetic shielding properties of the composites were assessed. The electrical conductivity of the composites increased with increasing filler addition, the 25 wt% filler mixture had the fastest curing rate and the maximum torque, and the continued increase in filler addition led to the occurrence of many defects. Concurrently, the alignment distribution of the filler progressively enhances with the augmentation of magnetic field strength. The composites synthesized at 180 mT exhibit the most substantial alignment, facilitating the establishment of effective conductive pathways. This results in a notable enhancement in electromagnetic shielding effectiveness, approximately 400% greater than that of pure silicone rubber and around 40% higher than that of non-aligned composites. This approach introduces a novel concept for shaping the structural design of flexible electromagnetic shielding materials. Highlights Highly loaded Fe 3 O 4 @RGO was prepared by self-assembly. The continuous phase structure of Fe 3 O 4 @RGO oriented was assessed. Fully oriented Fe 3 O 4 @RGO promotes the formation of conductive pathways. Fully oriented Fe 3 O 4 @RGO reduced the SER by 13% and increased the SEA by 67%.
研究领域:
材料科学
·
电磁学
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2025
·
IF 12.1
Min Zhang,Yongshun Song,Jun Wang,Xinlei Shi,Qiang Chen,Rui Ding,Junjie Mou,Haiping Fang,Yunlong Zhou,Ruoyang Chen
The biological effects of magnetic fields are pervasive in microorganisms, with significant attention given to alternating magnetic fields (AMFs). However, AMFs induce electrical and magnetothermal effects, which complicate the interpretation of magnetic field-induced biological effects and introduce uncertainties regarding cytotoxicity in practical applications. The static magnetic field (SMF) with few variables and high biocompatibility presents a promising alternative for both understanding biological mechanisms and ensuring safe applications, but has a remaining problem on weak interactions with microorganisms. Here we show that the combination of SMF with paramagnetic calcium-polypyrrole nanoparticles (Ca-PPy) remarkably enhances bactericidal activity. Our experiments indicate that the synergistic action of SMF and Ca-PPy significantly promotes the generation of reactive oxygen species (ROS), i.e., singlet oxygen and superoxide anion radicals, in Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), coupled with the physical disruption of bacterial membrane, exhibiting the extraordinary bactericidal performance (the bactericidal rate is over 94%). The mechanism disclosed by computations is that the singlet-to-triplet transition of radical pairs can be increased by the introduction of magnetic fields. These findings offer new insights into the biological effects of magnetic fields and pave the way for their safe, highly effective use in bactericidal applications.
研究领域:
微生物学
·
生物物理学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2018
·Vol. 35
·期 4
·1700418
·
IF 4.384
Ziyao Liu,Xiaohui Zhan,Xianghui Xu,Yao Wu,Zhongwei Gu
Superparamagnetic iron oxide nanoparticles (SPIONs) have become important tools for the imaging and detecting of prevalent diseases for many years. Scientists usually harness their attraction to a static magnetic field (SMF) to increase targeting efficiency and minimize side effects. To prolong blood circulation time and minimize reticuloendothelial system clearance, SPIONs are increasingly designed with a negatively charged surface. Understanding how a SMF affects the SPIONs with a negative surface charge is fundamental to any potential downstream applications of SPIONs as drug delivery carriers and bio-separation nanoparticles. The goal of our study is to investigate the effect of SMF treatment (204 mT) on the in vitro and in vivo protein corona formed on negatively charged SPIONs. The results reveal that the amount of protein and the composition of protein corona is directly related to the SMF treatment. Compared with the in vivo protein corona, SMF treatment exercises considerable influence on the composition of the in vitro protein corona. The in vitro protein corona formed on SPIONs modulates the secretion of inflammatory cytokines from cells. To the best of our knowledge, this report describes the first demonstration of a SMF as an influencing factor on protein corona formation in vivo. Our results help to elucidate the biological mechanisms of SPIONs with SMF treatment and suggest that the protein corona effect should be considered during the development of a magnetic target.
研究领域:
生物医学工程
·
纳米医学
·
分子生物学
✓ 已验证
DOI
高影响因子
Tissue Engineering Part A
·
2023
·
IF 4.1
Dr. Shujuan Guo,Mr. Weiguang Li,Prof. Weidong Tian,Prof. Yafei Wu
Periodontium is the rally of soft and hard tissues, which will be devastated continuously by the compromise of periodontitis. Current periodontal therapeutic methods cannot effectively reconstruct periodontal ligament (PDL), which is oriented at an angle with tooth root and combined hard tissues to form cementum-periodontal ligament-alveolar bone complex. Hence, it is urgent to find new techniques for PDL reconstruction to achieve functional regeneration of periodontium. Herein, we developed a novel method to manipulate the distribution and growth of periodontal ligament stem cells (PDLSCs) by utilizing highly paralleled static magnetic field (SMF) and magnetic nanoparticles (MNPs). PDLSCs were incubated with MNPs in vitro to label with them. Meanwhile, CCK8 and Live/dead cell staining assay were used to detect the impact of SMF and MNPs on cell viability. The directional migration and growth of PDLSCs were visualized under microscope. Furthermore, Real-time Quantitative PCR and western blot were utilized to calculate the expression level of periodontal ligament related genes. The results showed that PDLSCs could rapidly take up MNPs without compromising cell proliferation and viability, consequently endowed with the ability to respond via magnetic force. The cell migration analysis indicated that PDLSCs could move along the magnetic induction line, testifying that SMF exerted forces on PDLSCs that labeled with MNPs. It was demonstrated that collective application of SMF and MNPs not only induced PDLSCs organized and grew directionally, but also initiated elongation of cells and nucleus. Furthermore, the morphological alteration of the nucleus could also effectively enhance the gene and protein expression of Collagen Ⅰα2, Collagen Ⅲ and Periostin, suggesting the capability of PDLSCs to differentiate into PDL. In conclusion, this study exhibits a new approach for directional reconstruction of PDL to obtain physiological and functional regeneration of periodontium.
研究领域:
口腔科学
·
组织工程
·
干细胞生物学
·
磁生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomedicine-Nanotechnology Biology and Medicine
·
2021
·
IF 6.458
Yi He,Guanhui Chen,Ye Li,Yiming Li,Chen Yi,Xiliu Zhang,Hongyu Li,Binghui Zeng,Chao Wang,Weihong Xie,Wei Zhao,Dongsheng Yu
The biological behaviors of magnetic graphene oxide (MGO) in a static magnetic field (SMF) are unknown. The current study is to investigate the cellular behaviors, osteogenesis and the mechanism in BMSCs treated with MGO combined with an SMF. Results showed that the synthetic MGO particles were bio-compatible and could significantly improve the osteogenesis of BMSCs under SMFs, as verified by elevated alkaline phosphatase activity, mineralized nodule formation, and expressions of mRNA and protein levels. Under SMF at the same intensity, the addition of graphene oxide to Fe 3 O 4 could increase the osteogenic ability of BMSCs. The Wnt/β-catenin pathway was indicated to be related to the MGO-driven osteogenic behavior of the BMSCs under SMF. Taken together, our findings suggested that MGO under an SMF could promote osteogenesis in BMSCs through the Wnt/β-catenin pathway and hence should attract more attention for practical applications in bone tissue regeneration.
研究领域:
生物材料
·
组织工程
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jieqian Zhou,Qinxiu Sun,Zongyuan Han,Naiyong Xiao,Zefu Wang,Ouyang Zheng,Shucheng Liu
Refrigerator freezing (RF), immersion freezing (IF) and static magnetic field-assisted immersion freezing (MIF) were employed on golden pompano ( Trachinotus ovatus ), and the structure and functional properties of golden pompano myofibrillar proteins (MPs) during frozen storage were investigated. The results showed that the α -helix content, endogenous fluorescence intensity and total sulfhydryl content of the MIF samples during frozen storage were higher than those of the other frozen samples ( P < 0.05), indicating that MIF treatment inhibited the disordered protein structure changes and oxidation in protein caused by frozen storage. The emulsification properties showed that the MIF group formed and maintained the finest emulsion droplets during frozen storage. The low-field nuclear magnetic resonance (LF-NMR) results revealed that the immobilized water content ( P 21 ) of the MIF myofibrillar protein gel was 1.25 % and 2.48 % higher than that of the IF and RF samples after 180 days of frozen storage. These findings demonstrated that the MIF treatment contributed to the full swelling of MPs to form a dense gel structure, efficiently trapping water. In general, MIF could suppress the structural changes in myofibrillar proteins and the decrease the emulsification and gel properties of golden pompano caused by frozen storage.
研究领域:
食品科学
·
水产加工
·
蛋白质化学
·
低温生物学
·
食品贮藏与保鲜
·
分子构象分析
·
功能食品
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2024
·
IF 4.1
Zhou Xiujuan,Zhang Lin,Zhang Peng,Xu Hang,Song Jialei,Chang Yafei,Cai Tiantian,Xie Can
Static magnetic field (SMF) plays important roles in various biological processes of many organisms including plants, though the molecular mechanism remains largely unclear. Here in this study, we evaluated different magnetic setups to test their effects on growth and development on Arabidopsis (Arabidopsis thaliana), and discovered that plant growth was significantly enhanced by inhomogeneous SMF generated by a regular triangular prism magnet perpendicular to the direction of gravity. Comparative transcriptomic analysis revealed that auxin synthesis and signal transduction genes were upregulated by SMF exposure. SMF also facilitated plants to maintain the iron homeostasis. The expression of iron metabolism-related genes was downregulated by SMF, however, the iron content in plant tissues remains relatively unchanged. Furthermore, SMF exposure also helped the plants to reduce ROS level and synergistically maintain the oxidant balance by enhanced activity of antioxidant enzymes and accumulation of nicotinamide. Taken together, our data suggested that SMF is involved in regulating the growth and development of Arabidopsis thaliana through maintaining iron homeostasis and balancing oxidative stress, which could be beneficial for plant survival and growth. The work presented here would extend our understanding of the mechanism and the regulatory network of how magnetic field affects the plant growth, which would provide insights into the development of novel plant synthetic biology technologies to engineer stress-resistant and high-yielding crops.
研究领域:
植物生物学
·
分子生物学
·
转录组学
✓ 已验证
DOI
PubMed
高影响因子
Small
·
2019
·Vol. 15
·期 31
·1901617
·
IF 10.856
Xiyu Li,Qin Zou,Yi Man,Wei Li
To solve the clinical challenges presented by the long-term tracking of implanted hydroxyapatite (HA) bone repair material and to investigate the synergistic effects of superparamagnetic HA and a static magnetic field (SMF) on the promotion of osteogenesis, herein a new type of superparamagnetic/upconversion-generating HA material (HYH-Fe) is developed via a two-step doping method, as well as a specially-designed titanium implant with a built-in magnet to provide a local static magnetic field in vivo. The results show that the prepared HYH-Fe material maintains the crystal structure of HA and exhibits good cytocompatibility. The combined use of the superparamagnetic HYH-Fe material and SMF can effectively and synergistically promote osteogenesis/osteointegration surrounding the Ti implants. In addition, the HYH-Fe material exhibits distinct advantages in terms of both long-term fluorescence tracking and microcomputed tomography (micro-CT) tracking. The new material and tracking strategy in this study provide scientific feasibility and will have important clinical value for long-term tracking and evaluation of implanted materials and the bone repair effect.
研究领域:
材料科学
·
生物材料
·
生物医学工程
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 38
·7808-7826
·
IF 7.571
Peng Wang,Caili LV,Xiaosong Zhou,Zhenxu Wu,Zongliang Wang,Yu Wang,Liqiang Wang,Yongzhan Zhu,Min Guo,Peibiao Zhang
Wound healing is a complex process. Wound-repair materials require multiple functionalities, such as anti-inflammatory, antibacterial, angiogenesis, pro-proliferation, and remodeling. To achieve rapid tissue regeneration, magnetic field-assisted therapy has become a promising means. In this study, a homogeneous magnetic responsive nanocomposite hydrogel with enhanced mechanical properties was obtained through a tannin (TA)-assisted bridge between magneto-deformable cobalt ferrite nanoparticles (CFO NPs) and polyvinyl alcohol (PVA) matrix. In the presence of an external static magnetic field (SMF), the TA bridge could efficiently transmit magnetically actuated deformation to the PVA, which originated from the CFO NPs, generating a larger topographic change on the surface. The change of topography provided a mechanical cue to increase cell adhesion and proliferation. Moreover, due to the synergistic effects of TA modification and CFO NPs, the obtained magnetic responsive hydrogel exhibited considerable antibacterial activity. Furthermore, the results of in vivo study confirmed the anti-inflammatory properties of the TA-CFO/PVA hydrogel. More importantly, the TA-CFO/PVA hydrogel accelerated wound healing under a SMF, which contributed to the early vascularization induced by mechanical stimuli generated from the TA-CFO/PVA nanocomposite hydrogel. As a proof-of-concept, we provided an optimizing strategy for magneto-controlled skin tissue regeneration, which may have important guiding significance for the clinical application of magnetic field-assisted therapy.
研究领域:
材料科学
·
生物医学工程
·
组织工程
·
纳米技术
·
伤口愈合
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）