Kanamycin covalently grafted onto regenerated bacterial cellulose (RBC) membranes were constructed to prepare novel antimicrobial BC membranes. The prepared kanamycin grafted RBC membrane exhibits nanoporous three-dimensional network structure with a large surface area and high porosity. The antibacterial activity was explored and the result shows that the kanamycin grafted RBC membrane displays excellent antibacterial activity towards E. coli , C. albicans and S. aureus without any kanamycin leaching. Furthermore, MTT assay was carried out and the result demonstrates that the kanamycin grafted RBC membrane displays good biocompatibility.
Incorporating natural product antifoulants (NPAs) into coatings with controlled surface topography is considered a promising way to suppress marine fouling. However, the rapid leakage of NPAs and the relatively complicated process of constructing well-patterned topography remain unresolved problems for practical applications. In this work, capsaicin bonded to CoFe2O4/gelatin magnetic nanoparticles was mixed with a polydimethylsiloxane (PDMS)-based block copolymer. When applied together by a simple spray-coating method, these materials formed a film. The leakage of capsaicin was restrained by the chemical bonds with the CoFe2O4/gelatin nanospheres. The primary nanorough structure was constructed by the phase separation of the PDMS-based copolymer. The secondary nanorough structure was formed by the incorporation of capsaicin-loaded CoFe2O4/gelatin nanospheres, which were demonstrated to improve the orientation of the PDMS-based block copolymer chains. The combination of oriented nanotopography and nonleaking capsaicin endows the coating with enhanced, long-lasting antifouling ability.