Microbiome
·
2025
·Vol. 13
·期 1
·1-17
·
IF 12.7
Liu Youhua,Liu Jiaqi,Ren Ruiti,Xin Zimeng,Luo Yaojun,Chen Yushi,Huang Chaoqun,Liu Yuxi,Yang Tongyudan,Wang Xinxia
Background Although short-term high-fat diet (S-HFD) and long-term high-fat diet (L-HFD) induce metabolic disorder, the underlying epigenetic mechanism is still unclear. Results Here, we found that both 4 days of S-HFD and 10 weeks of L-HFD increased mRNA m 6 A level in epididymal white adipose tissue (eWAT) and impaired metabolic health. Interestingly, S-HFD activated transposable elements (TEs), especially endogenous retroviruses (ERVs) in eWAT, while L-HFD activated long interspersed elements (LINEs). Subsequently, we demonstrated that both S-HFD and L-HFD increased m 6 A level of Ehmt2 and decreased EHMT2 protein expression and H3K9me2 level, accounting for activation of ERVs and LINEs. Overexpression of EHMT2 in eWAT or inhibition of ERVs and LINEs by antiviral therapy improved metabolic health under HFD feeding. Notably, we found that both short-term and long-term HFD feeding increased Fimicutes/Bacteroidota ratio and decreased the gut microbiome health index. Fecal microbiota transplantation (FMT) experiments demonstrated that gut microbiota from S-HFD and L-HFD was responsible for increased m 6 A level in eWAT, resulting in glucose intolerance and insulin insensitivity. Furthermore, we identified that both S-HFD and L-HFD increased the abundance of the gut microbial metabolite homogentisic acid (HGA), and HGA level was positively correlated with unclassified_f__Lachnospiraceae which was both increased in S-HFD and L-HFD feeding mice. Administration of HGA increased the m 6 A level of Ehmt2 and decreased the EHMT2 protein expression and H3K9me2 level in eWAT, leading to metabolic disorder in mice. Conclusions Together, this study reveals a novel mechanism that S-HFD and L-HFD induce metabolism disorder through gut microbiota-HGA-m 6 A- Ehmt2 -ERV/LINE signaling. These findings may provide a novel insight for prevention and treatment of metabolism disorder upon short-term or long-term dietary fat intake.
Video
研究领域:
分子生物学
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营养学
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微生物学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2021
·
IF 4.242
Jia Xu,Shiwei Xie,Shuyan Chi,Shuang Zhang,Junming Cao,Beiping Tan
The increasing aquaculture production brought about the extensive use of high-lipid diets and antibiotics as effective means to spare costly protein and treat diseases, respectively. However dietary antibiotics and high-lipid diets both influence the gut microbiome . We hypothesized that antibiotics could affect the lipid metabolism by altering the composition of intestinal microbiota in fish at medium- and high-lipid diets. We explored this hypothesis by a 6-day administration of antibiotics mixtures to the diet of hybrid grouper ( Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂). Four diet groups were designed: medium-lipid (control diet, CD, 8.27% lipids), CD supplemented with antibiotics (vancomycin, neomycin sulfate, metronidazole ; ACD), high-lipid diet (HD, 15.32% lipids), and HD with antibiotics (AHD). The ACD and AHD groups exhibited significantly decreased crude lipids, lipid droplets, triglycerides , and total cholesterol contents of the liver (compared to CD and HD). The antibiotics treatment mainly upregulated the expressions of genes associated with lipolysis at the medium-lipid diet and downregulated the expression of lipogenesis-associated genes at the high-lipid diet. The antibiotics treatment mainly inhibited the expression of lipogenesis-associated proteins in the high-lipid diet, and mainly facilitated lipolysis in the medium-lipid diet. The antibiotics treatment significantly reduced the abundance and diversity of microbes, the abundance of Firmicutes , and Firmicutes / Bacteroidetes ratio. In addition, antibiotics homogenized the intestinal microbiota, which in turn caused analogous effects in medium- and high-lipid diets in grouper. In conclusion, short-term dietary antibiotics treatment improved the accumulation of lipids and lipid metabolism by inhibiting lipogenesis at the high-lipid level and facilitating lipolysis at the medium-lipid level via affecting the composition, diversity and functional pathways of gut microbiota.
研究领域:
水产养殖
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微生物学
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营养学
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分子生物学
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生理学
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产品关联
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 19
·3613
·
IF 5.2
Min Woo Kim,Yu-Jin Ham,Hyun-Bok Kim,Ji young Lee,Jung-Dae Lim,Hyun-Tai Lee
Recently, “mature” silkworms (MS) ofBombix morihave been considered a potential nutraceutical, with a number of health benefits reported for steamed and lyophilized MS powder (SMSP). However, no obesity-related effects have been reported for SMSP. In the present study, anti-obesity effects of SMSP were investigated in adult mice in vivo, aged 12 weeks at the onset of SMSP treatment, fed a normal diet (ND) and a high-fat diet (HFD), respectively, for 12 weeks. SMSP significantly suppressed body weight gain, intra-abdominal adipose tissue, and food efficiency in both ND-fed and HFD-fed adult mice. In addition, SMSP significantly decreased food intake and liver weight in HFD-fed mice, indicating that SMSP suppressed appetite and simultaneously reduced the conversion of feed into body weight in HFD-fed mice. SMSP also significantly lowered the serum levels of glucose, triglyceride, total cholesterol, low-density lipoprotein cholesterol, asparagine transaminase, alanine transaminase, and alkaline phosphatase. However, SMSP had no significant effect on the weights of the kidney, spleen, or thymus or the serum levels of blood urea nitrogen and creatinine. Taken together, the above results suggest that SMSP has potent anti-obesity effects and is safe for long-term use as a potential therapeutic and/or nutraceutical in both obese patients and non-obese individuals.Keywords:adipose tissue;appetite;body weight;Bombyx mori;high-fat diet;food intake;mature silkworm;normal diet;nutraceutical;obesity
研究领域:
营养学
·
医学
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产品关联
DOI
PubMed
高影响因子
Animal Nutrition
·
2024
·
IF 6.1
Jia Xu,Fan Wang,Chaoqun Hu,Junxiang Lai,Shiwei Xie,Kefu Yu,Fajun Jiang
High lipid diets (HLD) and high plant-protein diets (HPD) exhibit potential fishmeal-saving effects but negatively impact liver health and growth performance in fish. We hypothesized that HLD and HPD impair liver health in pearl gentian groupers ( Epinephelus fuscoguttatus ♂ × Epinephelus lanceolatus ♀) through the modulation of intestinal microbiota and bile acid (BA) metabolism. Four diet groups were tested: control diet (CD, 46.21% crude protein, 9.48% crude lipid), HLD (46.37% crude protein, 16.70% crude lipid), HPD (46.50% crude protein, 9.38% crude lipid), and high lipid-high plant-protein diet (HLPD, 46.54% crude protein, 16.67% crude lipid). A total of 300 fish (average body weight = 15.22 ± 0.03 g) were randomly divided into 4 diet treatments (ensuring 3 tanks replicates of each diet treatment, each tank containing 25 fish). After an eight-week feeding period, the HLD and HPD significantly decreased the final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR) and feed intake (FI) in comparison to CD group, with HLPD exacerbating these indicators ( P < 0.05). Compared to CD group, the content of total cholesterol (T-CHO) and triglyceride (TG) in liver and serum were significantly increased in HLD group ( P < 0.05). Compared to HPD group, the content of T-CHO in liver was significantly decreased, the content of TG in liver and serum were significantly increased in HLPD group ( P < 0.05). HLD, HPD, and HLPD impaired liver health by inducing histological damage, inflammation, and oxidative stress. Compared to CD group, the mRNA relative expression of bile salt export pump ( bsep ) and multidrug resistance protein 3 ( mdr3 ) were significantly increased in HLD group, whereas the mRNA relative expression of sterol-27-hydroxylase ( cyp27a1 ), microsomal epoxide hydrolase ( meh ), apical sodium-dependent bile acid transporter ( asbt ), multidrug resistance-associated protein 3 ( mrp3 ), farnesoid X receptor ( fxr ) and G protein-coupled bile acid receptor 5 ( tgr5 ) were significantly decreased ( P < 0.05). Compared to CD group, the mRNA relative expression of mdr3 , asbt , mrp3 , organic anion transporters 1 ( oatp1 ), meh , fxr and tgr5 were significantly decreased in HPD group ( P < 0.05). In summary, HLD affects intestinal microbiota, BA metabolism, and lipid metabolism, leading to lipid deposition and liver damage. HPD regulates gut microbiota, BA metabolism, inflammatory responses, and BA receptor expression, impairing grouper liver health. HLPD synergistically combines the adverse effects of HLD and HPD on grouper liver health.
研究领域:
水产养殖
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营养学
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生理学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cell Death Discovery
·
2025
·Vol. 11
·期 1
·1-16
·
IF 7
Liang Xue,Tian Ruofei,Li Ting,Wang Hao,Qin Yifei,Qian Meirui,Fan Jing,Wang Dan,Cui Hong-Yong,Jiang Jianli
Pancreatic cancer exhibits high mortality rates with limited therapeutic options. Emerging evidence suggests that the ketogenic diet may act as adjuvant therapy by triggering ferroptosis in cancer cells, though the underlying molecular mechanisms remain unclear. This study aims to investigate the molecular mechanisms linking ketogenic metabolism and ferroptosis, with an emphasis on key regulatory proteins. We demonstrated that pancreatic adenocarcinoma (PAAD) tissues significantly enhanced ketogenic and ferroptosis phenotypes compared to normal tissues, both correlating with poorer patient prognosis. These phenotypes showed strong interdependence mediated by CAV1. In the pancreatic tumor microenvironment, CAV1 was predominantly expressed in tumor cells. Through in vitro cell experiments, we clarified that Na-OHB downregulated CAV1 expression in pancreatic cancer cells, inhibiting the transcription of the CAV1/AMPK/NRF2 downstream ferroptosis-protective genes SLC7A11 and SLC40A1. Additionally, we demonstrated the interaction between CAV1 and SLC7A11 molecules; when CAV1 was downregulated, it affected the stability of SLC7A11, leading to the ubiquitination and degradation of the translated SLC7A11 protein. Through these dual mechanisms, Na-OHB caused Fe 2+ overload, lipid peroxidation accumulation, and oxidative stress in pancreatic cancer cells, ultimately triggering ferroptosis. In ketogenic diet-fed tumor-bearing mouse models, we also observed a significant increase in lipid peroxidation and other related biomarkers, while CAV1 and SLC7A11 levels were markedly decreased compared to the normal diet group. Our findings identify CAV1 as a pivotal molecular link between ketogenic metabolism and ferroptosis in pancreatic cancer. The multi-level regulatory axis involving CAV1-mediated transcriptional regulation and post-translational modifications provides mechanistic insights into ketogenic diet-induced ferroptosis, suggesting potential therapeutic targets for pancreatic cancer adjuvant treatment.
研究领域:
肿瘤学
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分子生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2023
·
IF 4
Feier Cheng,Yaru Yang,Shaojun Yun,Jinling Cao,Mingchang Chang,Yanfen Cheng,Cuiping Feng
Sparassis latifolia polysaccharide (SLP) has broad application prospects as a raw material of functional food. This study aimed to investigate the effects of SLP on liver cholesterol metabolism and its mechanism. Sprague–Dawley rats were fed a high-fat, high-cholesterol diet, and various assessments were conducted including hematoxylin and eosin staining, measurement of liver and metabolic indices, and antioxidant analysis. In addition, fluorescence quantitative reverse transcription-polymerase chain reaction was performed to analyze mRNA expression, and gas chromatography-mass spectrometry was employed for screening differential metabolites. The results demonstrated that SLP reduced the abnormal increase in the liver index caused by high fat and cholesterol and improved liver tissue morphology, metabolism level, and antioxidant-related enzyme activities. The polysaccharide regulated the liver sterol metabolism pathway, decreased the mRNA expression levels of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoAR) and sterol O-acyltransferase 1 (SOAT1), upregulated the mRNA expression levels of cholesterol 7α hydroxylase (CYP7α-1) and ABCA1, and increased the contents of propionic acid and butyric acid in the liver. These findings indicated a novel pathway through which SLP prevented hepatic lipid deposition and ameliorated sterol metabolism via regulating the HMG-CoAR-CYP7α-1 axis.
研究领域:
营养学
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生物化学
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分子生物学
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药理学
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产品关联
DOI
高影响因子
GENERAL AND COMPARATIVE ENDOCRINOLOGY
·
2018
·
IF 2.564
Xixi Song,Hongchao Jiao,Jingpeng Zhao,Xiaojuan Wang,Hai Lin
Ghrelin is one of the most important appetite regulating peptides, involved in the regulation of energy homeostasis. The role of ghrelin on the appetite and fat metabolism in chickens is different from that of ghrelin in mammals. Glucocorticoids and insulin are important hormones and work differently in energy regulation of body. In this study, the effects of dexamethasone (DEX, 2.0 mg/kg BW), subcutaneous insulin injection (40 µg/kg BW), and glucose load on ghrelin secretion and expression were determined in broilers. DEX treatment increased circulating ghrelin concentration in broiler fed with either a low-energy diet (11.05 MJ/kg of metabolizable energy) or a high-energy diet (14.44 MJ/kg of metabolizable energy). The expression levels of ghrelin were increased while both ghrelin and its receptor GHS-R1a expression levels were stimulated by DEX. A single subcutaneous insulin injection (40 µg/kg BW) or oral glucose infusion (2 g/kg BW) rise circulating ghrelin level. Ghrelin expression in the proventriculus was increased by insulin treatment but unchanged by glucose load. DEX had no detectable influence on ghrelin and GHS-R1a expression in the hypohtalamus, whereas insulin suppressed their expression. In conclusion, both insulin and glucocorticoid stimulate ghrelin secretion in chickens, in contrast to mammals. Glucocorticoids evoke peripheral ghrelin/GHS-R1a system while insulin increases peripheral ghrelin expression and suppress the activation of central ghrelin/GHS-R1a system. The result suggests that ghrelin involved in the modulating network of energy homeostasis in concert with glucocorticoids and insulin.
研究领域:
动物科学
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内分泌学
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营养学
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产品关联
DOI
PubMed
高影响因子
ENTOMOLOGICAL RESEARCH
·
2024
·Vol. 54
·期 4
·e12729
·
IF 1.2
Hui Jin,Lichao Zhong,Yifan Xu,Jie Shen
Deficiency of vitamin D3 has been found to be associated with an increased risk of developing some common diseases and death. However, the exact effect of vitamin D3 intake along with a high-protein diet and its optimal intake is unclear. The purpose of this work is to evaluate the beneficial effects of vitamin D3 on longevity using the Drosophila model. Vitamin D3 did extend lifespan on a high-protein diet, but above a certain concentration, it had the opposite effect in males. Our data suggest that the effect of vitamin D3 on longevity is diet-dependent and concentration-dependent.
研究领域:
营养学
·
衰老研究
✓ 已验证
产品关联
DOI
高影响因子
Microbiome
·
2023
·Vol. 11
·期 1
·1-13
·
IF 15.5
Ma Jie,Zhou Miao,Song Zehe,Deng Yuankun,Xia Siting,Li Yunxia,Huang Xingguo,Xiao Dingfu,Yin Yulong,Yin Jie
Background Compared to that of bacteria, the role of gut fungi in obesity development remains unknown. Results Here, alterations in gut fungal biodiversity and composition were confirmed in obese pig models and high-fat diet (HFD)-fed mice. Antifungal drugs improved diet-induced obesity, while fungal reconstruction by cohousing or fecal microbiota transplantation maintained the obese phenotype in HFD-fed mice. Fungal profiling identified 5 fungal species associated with obesity. Specifically, Ascomycota _sp. and Microascaceae_ sp. were reduced in obese mice and negatively correlated with fat content. Oral supplementation with fungi was sufficient to prevent and treat diet-induced obesity. Clec7a , which is involved in fungal recognition, was highly expressed in HFD-fed mice. The Clec7a agonist accelerated diet-induced obesity, while Clec7a deficieny in mice resulted in resistance to diet-induced obesity and blocked the anti-obese effect of antifungal drugs and fungi. Conclusions Taken together, these results indicate that gut fungi/ Clec7a signaling is involved in diet-induced obesity and may have therapeutic implications as a biomarker for metabolic dysregulation in humans.
Video Abstract
研究领域:
微生物学
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代谢生物学
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免疫学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2016
·
IF 4.839
Dezhen Wang,Xinru Wang,Ping Zhang,Yao Wang,Renke Zhang,Jin Yan,Zhiqiang Zhou,Wentao Zhu
Epidemiological studies indicate that exposure to persistent organic pollutants is positively associated with the prevalence of obesity. To delineate the potential role of technical-grade chlordane in obesity development, chlordane metabolism and chlordane-induced metabolic changes were investigated in mice fed high-fat diet (HFD) over a 6-week period. Gas chromatography–electron capture detector analysis showed that HFD induced more accumulation of technical chlordane in the liver, muscle and adipose tissue . The enantioselectivities of oxychlordane in selected tissues were also influenced by HFD. 1 H NMR-based liver metabolome indicated that technical chlordane can enhance the metabolic alterations induced by HFD. Compared with the low-fat diet (LFD) group, no differences were observed in the LFD + chlordane group. However, as many as 16 metabolites were significantly different between the HFD group and HFD + chlordane group. Moreover, compared to the LFD + chlordane group, the abundances of 24 metabolites significantly increased or decreased in the HFD + chlordane group. Twenty metabolites were altered in the HFD group compared to the LFD group. Tryptophan profiling suggested that both chlordane and HFD can disturb tryptophan catabolism. These interactions between technical chlordane and HFD suggest that technical chlordane is a candidate obesogen.
研究领域:
环境科学
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营养学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF NUTRITION
·
2023
·Vol. 62
·期 6
·2687-2703
·
IF 5
Zhuang Kejin,Meng Weihong,Shu Xin,Liang Defu,Wang Lidong,Zhang Dongjie
Purpose When blended, animal and plant proteins can complement each other in terms of amino acid composition and release time. In this study, we investigated whether the blended protein diet has a better feeding effect than the single protein diet, and to reveal the differences in growth and intestinal microbiota composition caused by the blended protein diet. Methods Forty Sprague Dawley (SD) rats received diets with different protein sources, including casein (C), whey protein (WP), black soybean protein (BSP), and black soybean-whey blended protein (BS-WP), for eight weeks. To investigate the effects of blended protein supplement on gut microbiota and metabolites, we performed a high throughput 16S rDNA sequencing and fecal metabolomics profiling. In addition, we determined growth and serum biochemical indices, and conducted intestinal morphology analyses. Results Compared to those in the BSP and WP groups, the daily body weight gain and feed conversion efficiency increased in the BS-WP group. Serum biochemical indices indicated that the protein utilization efficiency of the WP and BS-WP groups was relatively high, and the BS-WP blended protein diet improved the protein adoption rate. The BS-WP blended protein diet also improved intestinal tissue morphology and promoted intestinal villi development compared to the single protein diets. Furthermore, dietary protein altered the composition of gut microbiota, the gut microbial diversity of rats fed with the BS-WP diet was significantly ( P < 0.05) higher than that of the other groups. The difference in dietary protein corresponded with an alteration of fecal amino acids and their metabolites, and tryptophan and tyrosine metabolism were the key mechanisms leading to the changes in fecal microbial composition. Conclusion Dietary protein sources played an important role in the growth and development of rats by influencing intestinal metabolism and microbial composition. The BS-WP blended protein diet was more conducive to nutrient absorption than the single protein diet. Furthermore, blended protein increased the diversity of intestinal microbes and aided the establishment of intestinal barrier function.
研究领域:
营养学
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微生物学
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代谢组学
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动物科学
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产品关联
DOI
PubMed
高影响因子
Journal of Ocean University of China
·
2015
·Vol. 14
·期 4
·695-702
·
IF 0.558
Xu Houguo,Huang Lina,Liang Mengqing,Zheng Keke,Wang Xinxing
A 3-month feeding experiment was conducted in an in-door seawater system to investigate the effect of dietary vitamin E (Ve) on the sperm quality of turbot ( Scophthalmus maximus ). D-α-tocopherol acetate was supplemented to the basal (control) diet (65.14 mg kg −1 Ve) to obtain low and high levels of dietary Ve (244.60 mg kg −1 , LVe; 721.60 mg kg −1 , HVe). Compared with the control, sperm concentration was significantly increased in Ve-supplemented groups (LVe and HVe); while relative sperm volume and testis-somatic index were significantly increased in group HVe only. Sperm motility duration was significantly longer in group HVe than in the control, but no significant difference was observed in percent motility among groups. Sperm size, the uniformity of mitochondrial size, and the integrity of mitochondria cristae and plasma membrane were improved by dietary Ve, especially in HVe. The content of Ve in testis and liver as well as polyunsaturated fatty acids in sperm increased with dietary Ve. These results suggested that dietary Ve, especially at the high level (721.60 mg kg −1 ), significantly improved sperm concentration and motility duration and maintained normal sperm morphology of turbot.
研究领域:
水产养殖学
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营养学
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生殖生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）