JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2019
·
IF 2.298
Zhiying Wu,Weiling Li,Ping Yu,Xiaoyu Fan,Hua Sun,Hua Zhao,Yue Zhang
The solubility of marbofloxacin in 12 pure solvents (methanol, ethanol, isopropanol, n-propanol, n-butanol, tert-amyl alcohol, acetonitrile, ethyl acetate, acetone, toluene, tetrahydrofuran, and 1,4-dioxane) at T = 283.15 to 328.15 K and P = 101.3 kPa was measured by a dynamic laser monitoring method. Results of these experiments demonstrate that the solubility of marbofloxacin in those 12 pure solvents increased with the increase of temperature. The experimental results showed that the solubility of marbofloxacin in the pure solvents decreased in the order of tetrahydrofuran > acetone > ethyl acetate > methanol ≈ acetonitrile (before 305.30 K) or > methanol ≈ acetonitrile > ethyl acetate (behind 305.30 K) > 1,4-dioxane > n-butanol > n-propanol > ethanol > tert-amyl alcohol > isopropanol > toluene. The Apelblat model, λh model, and nonrandom two-liquid (NRTL) model were used to correlate with the experimental solubility data. Compared with the λh model and NRTL model, the Apelblat model has a better correlation with the experimental values. The experimental solubility data and the correlation model in this study can be helpful for the related scientific research and scale-up production.
研究领域:
药学
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化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yuanyuan Duan,Yongli Yu,Limei Wang,Shuang Zhao
In this work, a facile method for the preparation of dual-emission lanthanide nanoparticles (NPs) was developed. Tb 3+ -doped LaF 3 NPs coated with 2,6-pyridinedicarboxylic acid (DPA) and Eu 3+ , namely LaF 3 :Tb@DPA-Eu NPs, could produce characteristic emissions of Tb 3+ and Eu 3+ under 272 nm excitation, which originated from the absorption of DPA. The LaF 3 :Tb@DPA-Eu NPs with tunable emission peaks and color could be synthesized by changing the concentration of Eu 3+ . Additionally, the obtained NPs were comprehensively characterized by various analytical methods. Transmission electron microscopy (TEM) images showed that the average size of the NPs was ~15 nm. Moreover, X-ray diffraction (XRD) analysis revealed that the products were single-phase LaF 3 , while the doped Tb 3+ entered the LaF 3 lattice to occupy the La 3+ sites. Owing to their excellent water solubility and good optical properties , the LaF 3 :Tb@DPA-Eu NPs were used as a fluorescence probe for the detection of marbofloxacin (MAR) based on the fluorescence quench. Notably, an effective ratiometric fluorescence method was established. The approach exhibited good sensitivity and selectivity with a linear range of 0.10 ~ 10.0 μM and detection limit of 85 nM. It was successfully employed for the determination of MAR in milk samples with recoveries of 98.1%–106%. Thus, the fluorescence method based on the LaF 3 :Tb@DPA-Eu NPs developed herein provides a convenient and fast platform for the detection of MAR.
研究领域:
分析化学
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材料科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
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IF 2.931
Rui Zhao,Shuyun Bi,Di Shao,Xiaoyue Sun,Xu Li
A novel surface enhanced-Raman spectroscopy (SERS) assay for marbofloxacin was developed based on β -cyclodextrin-modified silver nanoparticles ( β -CD-AgNPs). The marbofloxacin could interact with β -CD-AgNPs and a new assembly was formed by Ag N covalent bond . This assembly was characterized by the spectra of FT-IR and UV–vis. The optimal measurement conditions were studied in detail. In 0.033 mol L −1 HCl solution, marbofloxacin had a sensitive SERS signal at 806 cm −1 . The enhancement factor (EF) was 2.11 × 10 7 . There was a good linear correlation between the concentration of marbofloxacin and SERS intensity: the linear range was 0.003–0.03 μmol L −1 ( r 2 = 0.996). The limit of detection (LOD) (S/N = 3) was 1.7 nmol L −1 (S/N = 3). Moreover, the influence of some interferences including Cu 2+ , K + , Zn 2+ , Ca 2+ , Na + , Mg 2+ , glucose and tiamulin on the determination were studied. The developed SERS method was used to detect the content of marbofloxacin in chicken and duck, the recovery was 101.3%–103.1% with RSD 4.07%–6.83%.
研究领域:
分析化学
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兽医药学
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DOI
PubMed
高影响因子
LUMINESCENCE
·
2021
·Vol. 36
·期 4
·977-985
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IF 2.464
Xu Li,Yue Yuan,Rui Zhao,Di Shao,Shuyun Bi
To assess the toxicity of residual marbofloxacin from animal-derived food, the interaction characteristics of marbofloxacin to bovine/human serum albumins (BSA/HSA) were explored using spectroscopic methods combined with molecular modelling. According to fluorescence spectra and time-resolved fluorescence spectra measurements, quenching of BSA/HSA fluorescence induced by marbofloxacin was characterized as static quenching. A 1:1 ground-state complex of marbofloxacin to BSA/HSA was formed with binding constant ( K a ) 1.66 × 10 4 /9.74 × 10 3 M −1 at 291 K. The location of marbofloxacin binding at site I within BSA/HSA was clarified by site marker competitive experiments. Molecular modelling demonstrated that the binding region for marbofloxacin to BSA and HSA were at site I with the lowest binding free energies of −22.86 and −21.60 kJ mol −1 , respectively. Hydrogen bonds and van der Waals forces were dominantly involved in the spontaneous binding. Nonradiation energy transferred from BSA and HSA to marbofloxacin, due to the close distance ( r 0 ) between marbofloxacin and Trp residues of BSA (4.28 nm) and HSA (3.34 nm). As explained by circular dichroism (CD) spectra, an increased BSA/HSA α- helix structure was observed after binding to marbofloxacin. Ultraviolet–visible (UV–vis) and Fourier transform infrared (FT-IR) spectra suggested that conformation of the two proteins was altered by marbofloxacin.
研究领域:
药理学
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生物化学
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食品安全
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Tianyu Wang,Yonghui Xia,Xuan Wan,Yafeng Zhang,Nayu Chen,Yuan Jin,Guangli Li
Improper and persistent use of marbofloxacin (MAR) is prone to produce residues that deteriorate the surrounding environment and endanger our health. Hence, it is urgent to stablish a facile and efficient detection technique for the detection of MAR . Herein, a highly efficient electrochemical platform was constructed for sensitive determination of MAR using zirconium-based metal–organic framework (UiO-66)/nitrogen-doped graphene (NG) nanocomposites (UiO-66/NG). The surface morphology, crystalline phase, elemental composition, and electrochemical behavior of UiO-66/NG were characterized by various spectroscopic and electrochemical techniques. Owing to the synergistic interaction of UiO-66 MOFs and NG nanosheets, the UiO-66/NG exhibited remarkable catalytic activity for MAR oxidation. As consequently, the UiO-66/NG demonstrated a wide linear response range (0.01 − 10 μM), high sensitivity (1.80 μM μA −1 cm −2 ) and low detection limit (0.002 μM). The proposed UiO-66/NG/GCE demonstrated robust voltammetric responses even in the presence of excess potential interfering species and retained stable response signals for at least two weeks. More importantly, the proposed UiO-66/NG/GCE displayed highly consistent sensitivity toward MAR detection in both aquaculture water and milk matrices and PBS buffer, and achieved accurate and sensitive detection of MAR with good recovery. Together with its low cost and facile operation, the UiO-66/NG/GCE shows great prospects for trace detection of MAR from complex matrices.
研究领域:
分析化学
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环境科学
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医学检测
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DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2025
·
IF 4.6
Xiujin Chen,Biao An,Minghui Dai,Pingping Xiong,Yubing Bai,Huawei Niu,Zhaozhou Li,Yao Wang
Marbofloxacin (MBF) is widely used in veterinary medicine for the prevention and treatment of various infectious diseases. However, improper use of MBF may leave residues in edible animal tissues and milk, posing a potential hazard to human health. Here, we developed a fluorescence and colorimetric dual-mode immunoassay for the detection of MBF based on the inner filter effect between gold-silver bimetallic nanoclusters (AuAgNCs) and the oxidation products of 3,3',5,5'-tetramethylbenzidine (oxTMB). In this immunoassay, horseradish peroxidase (HRP) catalyzes the oxidation of TMB to oxTMB, which can further quench the fluorescence of AuAgNCs. Thereby, the color change of oxTMB and the fluorescence change of AuAgNCs can be utilized as the dual signals for MBF analysis. Under optimal conditions, the detection limit of the fluorescence enzyme-linked immunosorbent assay was 9 ng L -1 , with a 50% inhibitory concentration (IC50) of 0.80 µg L -1 . Additionally, this immunoassay demonstrated high specificity for MBF and satisfactory recovery rates in pork samples (fluorescence mode: 84.00-104.40%; colorimetric mode: 90.54-107.63%) and milk samples (fluorescence mode: 88.00-104.00%; colorimetric mode: 86.15-115.22%). Hence, the developed immunoassay has great application potential for sensitive detection of MBF residues in foods.
研究领域:
分析化学
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食品安全
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免疫学
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纳米生物技术
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药物残留检测
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DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2017
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IF 1.392
Xiujin Chen,Zhaozhou Li,Jinying Guo,Daomin Li,Hongli Gao,Yao Wang,Chuanlai Xu
A broad-specificity immunoassay was established to monitor marbofloxacin (MBF) and ofloxacin (OFX) residues in raw milk and porcine muscle samples. The main influential parameters in the assay buffer were investigated to improve the assay performance. The cross-reactivities (CRs) were lower than 0.25%, except for OFX (41.38%). The quantitative working ranges for MBF and OFX based on fortified matrices ranged from 0.11 to 30.85 ng mL−1, with a 50% inhibitory concentration of 0.76±0.12 ng mL−1 for MBF and 2.70±0.28 ng mL−1 for OFX. The detection limits of enzyme-linked immunosorbent assay (ELISA) ranged from 0.053 to 0.358 ng mL−1 in spiked samples. The mean recoveries were in the range of 80.52–95.46%, less than 12.06% of the coefficient of variation. A good correlation between the ELISA results and those from instrument methods demonstrated the practical application of the novel ELISA. Therefore, the immunoassay proposed is feasible for MBF and OFX analysis in animal-derived foods.
研究领域:
食品安全
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免疫学
·
分析化学
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DOI
高影响因子
BMC Veterinary Research
·
2015
·Vol. 11
·期 1
·1-7
·
IF 1.777
Qu Ying,Qiu Zhenzhen,Cao Changfu,Lu Yan,Sun Meizhen,Liang Chaoping,Zeng Zhenling
Background Marbofloxacin is a third-generation fluoroquinolone developed solely for veterinary medicine with a broad spectrum of antibacterial activity against some Gram-positive and most Gram-negative bacteria, including the bovine respiratory tract pathogen, Pasteurella multocida. The objective of our study was to elucidate the pharmacokinetics and pharmacodynamics of marbofloxacin in a Pasteurella multocida infected murine lung model, and to estimate the magnitudes of pharmacokinetics-pharmacodynamics parameters associated with various effects.Results The pharmacokinetic studies revealed marbofloxacin kinetics in infected mice were linear over a dose ranging from 1.25 to 10 mg/kg of body weight. The protein binding in the plasma of neutropenic infected mice was 29.77 %. The magnitudes of the ratio of the free-drug area under the concentration-time curve over 24 h to MIC (fAUC0-24h/MIC) associated with static effect, a 2 log10 reduction and a 3 log10 reduction of bacterial counts were 40.84, 139.34, and 278.08 h, respectively.Conclusion sBased on the dose range study, marbofloxacin exhibited concentration-dependent killing and the fAUC/MIC was the PK/PD index that correlated best with efficacy (R2 = 83 %). On the basis of a bactericidal effect goal of fAUC0-24h/MIC of 278.08 h, if marbofloxacin is used for the treatment of P. multocida serious lung infection with an MIC90 of 0.12 μg/ml, the current dose (2 mg/kg) would fail to achieve a bactericidal effect. It would benefit from higher doses (4 ~ 6 mg/kg) than those commonly used in clinical practice.
研究领域:
兽医学
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微生物学
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药物研究
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DOI
PubMed
高影响因子
Materials Today Chemistry
·
2025
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IF 6.7
Xiaoman Qi,Yonghui Xia,Jingtao Wu,Xuan Wan,Tianyu Wang,Qing Li,Guangli Li
The abuse of marbofloxacin (MBF) leaves residues in foods and environment. Therefore, developing a selective method for trace determination of MBF is of great significance. Herein, a bimetallic Au@Ag nanoparticle-decorated black phosphorus nanosheet-supported molecularly imprinted polymer film (MIP/BPNS-Au@AgNP) was fabricated for label-free and robust MBF sensing. In the presence of MBF, a polypyrrole film was grown onto the surface of BPNS-Au@AgNPs via potentiodynamic scanning. Au@AgNP functionalization prevented BPNS from degrading in aqueous solutions, enhanced its electrical conductivity, and provided a larger surface area to accommodate more selective imprinted cavities. Hence, the MIP/BPNS-Au@AgNP film demonstrated excellent electrochemical activity for MBF oxidation, including a broad linear dynamic concentration range (0.001–10 μM) and a low limit of detection (0.13 nM). Moreover, the MIP/BPNS-Au@AgNP film exhibited outstanding signal discrimination ability against potential interfering species and retained robust responses for four weeks. The MIP/BPNS-Au@AgNP film could also determine trace amounts of MBF in milk, sewage, and serum.
研究领域:
分析化学
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环境科学
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食品科学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Tianci Lv,Fubin Liu,Mengqian Xiao,Ying Liu,Litao Wang,Guihua Gao
In this study, a novel hierarchical pore MIL-101(Cr) foam (HPF-MIL-101) was designed and prepared using the sacrificial template method with NaCl as the sacrificial template. This method involved grinding, heating, and washing the NaCl template to produce HPF-MIL-101, with PVDF as the binder and MIL-101(Cr) as the adsorbent. This preparation process is both straightforward and cost-effective, avoiding the use or generation of any organic reagents, thereby offering an environmentally sustainable approach for producing metal-organic framework (MOF) composites. The prepared HPF-MIL-101 exhibited excellent adsorption capabilities for both anionic dye (methyl orange, MO) and cationic dye (methylene blue, MB). The adsorption process followed a pseudo-second-order kinetic model and Friedrich isotherm model, indicating a multilayer adsorption. This is further supported by the Weber-Morris intraparticle diffusion model, which divided the adsorption process into three stages. Furthermore, the adsorption process was consistent with the Freundlich isotherm model, with a correlation coefficient ( r ) greater than 0.96. HPF-MIL-101 can also be used as an adsorbent for solid phase extraction (SPE). Therefore, an SPE method combined with high-performance liquid chromatography (HPLC) was developed using HPF-MIL-101 as the adsorbent to analyze five fluoroquinolones (FQs) in water samples. This analytical method showed good linearity in the range of 30–2000 ng·mL −1 , with excellent linear correlation coefficient ( r = 0.9991–0.9999), reasonable extraction recoveries ranging from 80.39 to 112.7 % (RSD ≤ 7.9 %), and low limits of detection (8–30 ng·mL −1 ). Overall, the results indicated that HPF-MIL-101 not only had a simple, environment-friendly, and pollution-free preparation process but also can be reused for enrichment and detection of trace FQs in water. Thus, HPF-MIL-101 exhibits immense application potential in environmental pollutant removal and also provides a valuable reference for the preparation and application of other MOF composites.
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Furong Tang,Ting Zou,Ziyi Wang,Juan Zhang
A novel fluorinated triazine-based covalent organic frameworks (F-CTFs) was designed and synthesized by using melamine and 2,3,5,6-tetrafluoroterephthalaldehydeas as organic ligands for selective pipette tip solid-phase extraction (PT-SPE) of amphiphilic fluoroquinolones (FQs). The competitive adsorption experiment and mechanism study were carried out and verified that this F-CTFs possesses favorable adsorption affinity for FQs. The abundant fluorine affinity sites endowed the F-CTFs high selectivity to FQs extraction through F-F interactions. The adsorption capacity of F-CTFs can reach up to 109.1 mg g −1 for enrofloxacin. The detailed characterization of the F-CTFs adsorbent involved the application of various techniques to examine its morphology and structure. Under optimized conditions, a method combining F-CTF-based PT-SPE with high-performance liquid chromatography (PT-SPE-HPLC) was established, which exhibited a broad linear range, excellent precision, and an impressively low limit of detection, and could be used for the determination of six FQs in milk, with LODs as low as 0.0010 μg mL −1 . The recovery rates during extraction varied between 92.1% and 111.4%, exhibiting RSDs below 6.8% at different spiked concentrations.
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
CRYSTAL GROWTH & DESIGN
·
2024
·
IF 3.2
Yujiao Li,Yunan Zhang,Qi An,Jingwen Shi,Lixin Liu
Based on the study of the effect of positional isomerism of coformer functional groups on the cocrystallization and physicochemical properties of the active pharmaceutical ingredients, the impact of cis–trans isomeric butenedioic acid as coformers on the conformation, crystal structure, and its physicochemical properties of marbofloxacin was further explored. In this work, fumaric acid (FA) and maleic acid (MA) with different configurations were chosen as coformers to synthesize the pharmaceutical salt cocrystal (MBF-FA-H2FA) and salt (MBF-MA) of marbofloxacin (MBF), and their structures were fully characterized. Significant differences between the conformations of marbofloxacin in the salt cocrystal and in salt were found. In the salt cocrystal, the N atom of the piperazine group from marbofloxacin is coplanar with the pyridone ring, and the whole is straight like fumaric acid, whereas the marbofloxacin piperazine group in the salt is bent like the maleic acid configuration. Furthermore, the conformational variability of marbofloxacin in the salt cocrystal and the salt resulted in different crystal structures and opposite physicochemical properties. Notably, both multicomponent crystals have a surface hydrophilic intercalation structure. However, the salt cocrystal and salt exhibited different solubility and permeability. Specifically, the MBF-MA salt showed improved solubility and permeability, while the MBF-FA-H2FA salt cocrystal showed a decreased solubility and permeation rate compared to MBF. In addition, in vitro bacterial inhibitory activity assays indicated that the MBF-MA salt has stronger inhibitory activity against Gram-negative and Gram-positive bacterial strains than the MBF-FA-H2FA salt cocrystal and pure MBF.
研究领域:
药学
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晶体学
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生物化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）