Ying Chen,Wenyi Zhao,Ziyang Liu,Huan Zhou,Zhen Yang,Lei Yang
The high incidence of bone trauma has necessitated the development of multifunctional biomaterial implants with both hemostatic and osteogenic properties to address clinical challenges such as bone nonunion and immune rejection associated with nondegradable bone hemostatic materials. In this study, starch/bioactive glass/GelMA-based self-assembling composite microspheres were developed as a novel bone trauma filler exhibiting dual functions of efficient hemostasis and bone regeneration. These micron-scale composite microspheres can be applied to bone trauma sites during surgical procedures or emergency situations. Upon absorption of blood, self-assembly occurs rapidly through hydroxyl/amino-mediated interfacial interactions, preventing dispersion by blood and facilitating the formation of a stable barrier for emergency hemostasis. Furthermore, the bioactive glass component promotes osseointegration via biomineralization-mediated assembly, while the released bioactive ions enhance the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. This composite microsphere exhibited biodegradable properties and significantly improved cranial bone defect repair in vivo.
Ca-silicate (CSi) bioceramics have garnered significant interest in bone tissue engineering but their multifunction and biodegradation are suboptimal for some emergent bone trauma conditions. Foreign ion doping and core-shell architectural design offer promising strategies to optimize osteogenic efficacy while precisely regulating degradation kinetics and biologically functional ion release. Herein we developed the core-shell porous bioceramics via coaxial nozzle system, featuring a P-doping wollastonite (CSi-P) core and Zn-doping wollastonite (CSi-Zn)/β-tricalcium phosphate (β-TCP) shells. A 10 % porogens in the shell layer would enable controllable micropore architecture. In vitro studies demonstrated that Zn doping could finely tune the CSi-shell degradation rates, governing both physical dissolution and sustained release of bioactive ions. The CSi-based components further exhibited superior biomimetic remineralization capability in simulated body fluid. Meanwhile, both P12@Zn8 and P12@Zn12 exhibited remarkable antibacterial potential against Gram-positive bacteria ( S. aureus ). In vivo mandibular defect experiments revealed that the CSi-Zn granules outperformed β-TCP counterparts in bone repair at 10 and 16 weeks interval. Notably, the P12@Zn8 formulation achieved optimal degradation-osteogenesis coupling, exhibiting enhanced trabecular bone formation and complete repair within 16 weeks. This core-shell design strategically balances tunable degradation with spatiotemporal bioactivity, and may provide a solution to the problem of matching the absorption time of the materials with the bone regeneration time in clinical practice. Our findings highlight the potential of compositionally graded core-shell bioceramics as next-generation bioactive implants for emergent bone trauma regeneration and repair.
Fengbo Yu,Hongyuan Wang,Qiang Wang,Fengguo Zhai,Jinghua Wang,Chunming Huang,Liao Cui
Tanshinol (Tan) has good therapeutic effects on osteoporosis, fracture, and bone trauma repair. However, it is easily oxidised, has low bioavailability and a short half-life. To solve these problems, the study aimed to develop a novel bone-targeted nano-sustained-release drug delivery system PSI-HAPs for the systemic administration of Tan. This proposed system has hydroxyapatite (HAP) as the core to load drug and polysuccinimide (PSI), PEG-PSI (Polyethylene glycol, PEG), and ALN-PEG-PSI (Alendronate sodium, ALN) as the coating materials to form nanoparticles. The article examines the various PSI-HAPs’ entrapping efficiency (EE, %), drug loading capacity (DLC, %), and distribution to determine the best PSI-HAP formulation in vivo. The in vivo experiment showed that the ALN-PEG-PSI-HAP (ALN-PEG/PSI molar ratio = 1:20) was the best preparation due to its higher distribution on bone (120 h) and lower distribution in the other tissues. The determined preparation was a uniformly spherical or sphere-like nanoparticle with a negative zeta potential. Additionally, it exhibited pH-sensitive drug release in PBS based on an in vitro drug release test. The proposed PSI-HAP preparations were prepared in the water solution using a facile preparation process without ultrasound, heating, and other conditions, which can significantly affect the stability of drugs.
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