Anming Wang,Xinxin Chen,Jianyun Yu,Ningning Li,Huimin Li,Youcheng Yin,Tian Xie,Stephen Gang Wu
To prepare enzyme@Ca 3 (PO 4 ) 2 for environmentally friendly biocatalysis, Ca 2+ and (PO 4 ) 3− were extracted from bone waste by acidification. In the nearly neutralized filtrate, the Ca 2+ and (PO 4 ) 3− formed a Ca 3 (PO 4 ) 2 sediment that crystallized on a template of Thermomyces lanuginosus lipase (TLL) at 4 ℃ for 24 h, producing enzyme@Ca 3 (PO 4 ) 2 -Bone hybrid nanoflowers (hNFs). Clindamycin palmitate was efficiently synthesized by transfer of a palmityl moiety from vinyl palmitate to clindamycin free base using these hNFs as a biocatalyst. At 30 ℃ in petroleum ether (PE) as the solvent, the yield of the TLL hNF-catalyzed reaction was as high as 70.0%. Even at a high temperature (80 ℃), the yield in the hNF-catalyzed reaction was still 52.6%, but no product was detected when using free lipase as the catalyst. Moreover, the hNFs retained 90% of their initial activity after 10 cycles (120 h, 12 h per cycle). This green and sustainable method that utilizes bone waste from food production as the raw source of the inorganic component was facile and efficient, and the system may also be applicable for preparing other enzyme@Ca 3 (PO 4 ) 2 hNFs for industrial applications.
Xingquan Xiong,Zhongke Tang,Zhaohong Sun,Xiaoqing Meng,Sida Song,Zhilong Quan
An eco-efficient, green, and multi-gram procedure is presented for one-pot multicomponent synthesis of N -substituted 1,2,3-triazoles by using waste fishbone powders supported CuBr (FBPs-CuBr) as catalyst. FBPs-CuBr is found to be an efficient heterogeneous catalyst and a series of 1,2,3-triazoles are obtained in moderate to excellent yields in water under MW irradiation (70–98%). It can be separated conveniently by a simple filtration and reused at least seven consecutive runs with a slight drop in the product yields. Furthermore, the desired product still could be obtained in 80% yield when the scale of the reaction was increased to 40.0 mmol.
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