COMPOSITES PART B-ENGINEERING
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2022
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IF 11.322
Shuyi Li,Rongli Deng,Xuenong Zou,Qiong Rong,Jiali Shou,Zilong Rao,Wanqiu Wu,Gang Wu,Daping Quan,Miao Zhou,Tim Forouzanfar
Periosteum is crucial to the initial healing of bone defects because it provides a stable periosteum-specific microenvironment and abundant osteogenic cells towards bone repair. However, the usage of autologous periosteum is restricted for limited availability. Xenogeneic decellularized periosteum (DP) preserves periosteum-specific cues but the hardly-controlled physicochemical properties render it inconvenient to use widely. Our study aimed to fabricate a tissue-engineered periosteum (TEP) with a continuous periosteum-specifically bioactive surface and tailored physicochemical properties by co-axial electrospinning of poly(ε-caprolactone) (PCL)/periosteal decellularized extracellular matrix (dECM) to promote bone defects healing. Before use, DP was confirmed for effective removal of residual DNA and well preservation of biological components, e.g., collagen and glycosaminoglycans . After optimization of fabrication processes, PCL/dECM co-axially electrospun membrane (PEC) bore an exquisite core-shell structure, which was effectively equipped with composite advantages of dECM and PCL. Notably, PEC stood out in terms of tensile strength and long-term durability within a physiological environment compared to that of the dECM uniaxial electrospun membrane (ECM). Besides, PEC exhibited remarkably better cell proliferation , migration, bio-mineralization, and osteogenic properties as compared to that of the electrospun PCL membrane. Moreover, PEC-TEP could significantly enhance the recovery of critical-sized bone defects in rats than that of PCL-TEP. To the best of our knowledge, biomimetic PEC-TEP was fabricated and used for the first time to repair bone defects. This novel strategy is promising to fabricate a dECM-based advanced membrane for tissue engineering .
研究领域:
组织工程
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生物材料
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再生医学
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骨科
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2024
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IF 18.5
Zhengwei Li,Shun Li,Chongjian Gao,Juan Liu,Huawei Qu,Jirong Yang,William Weijia Lu,Changshun Ruan,Xufeng Niu
The scaffold that bioinspired natural bone-periosteum is ideal for the repair of bone defects, while the achievement of a gradient scaffold with an integrated and stable interface remains challenging. Herein, a bioinspired bone-periosteum integrated collagen-based scaffold is developed, in which the top layer is electrospun collagen-dense scaffold as bioinspired periosteum (BP) to prevent invasion of reticular fiber tissue and the bottom layer is in situ mineralized collagen scaffold (IMCS) to promote osteogenic differentiation. Owing to the proposed continuous manufacturing of successive 3D printing and electrospinning, the integrated scaffold (BP-IMCS) comprised of BP and IMCS demonstrates excellent structural stability, ten times higher than that of direct-combination scaffolds. Besides, in vivo implantation results confirmed that BP-IMCS significantly improves new bone formation up to 32.47%, better than an individual layer, due to its co-work of mineral ions and bioinspired structure. Therefore, this study offers a continuous manufacturing strategy to realize the integrated and interface-stable bone-periosteum structure, providing new solutions for heterostructure tissue fabrication.
研究领域:
生物材料学
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组织工程学
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再生医学
✓ 已验证
DOI
高影响因子
Acta Biomaterialia
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2024
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IF 9.4
Jintao Zhong,Wenhua Li,Hetong Li,Jin Zhang,Zuoxu Hou,Xiao Wang,Enhui Zhou,Ke Lu,Weida Zhuang,Hongxun Sang
The periosteum, a highly specialized thin tissue, is instrumental in contributing to as much as 70% of early bone formation. Recognizing the periosteum's vital physiological roles, the fabrication of a biomimetic periosteum has risen as an auspicious strategy for addressing extensive bone defects. In the study, we obtained such biomimetic periosteum by utilizing periosteum-derived stem cells (PDSCs) spheroids. These spheroids are induced to spontaneously generate a bioactive membrane on a delicate 3D-printed polycaprolactone (PCL) substrate. This process yields a biomimetic periosteum rich in the resources needed for bone repair. The in vitro evaluations demonstrated that this membrane can act as a repository for growth factors and stem cells. The release kinetics confirmed a sustained delivery of BMP-2 and VEGF, which promoted enhanced osteogenesis and angiogenesis in vitro , respectively. The in vivo results further highlighted robust bone regeneration from critical cranial defects upon the application of this biomimetic periosteum. The biomimetic periosteum, easily harvested and potent in bioactivity, presents substantial clinical potential, particularly for the treatment of critical-sized bone defects. Statement of Significance PDSC theoretically demonstrates substantial potential in membrane construction, a value we've harnessed in this pioneering application. By employing cell spheroids, we've successfully integrated a substantial number of cells into the membrane framework. PDSC spheroids exhibit the remarkable ability to self-assemble into functional membranes, endowing them with robust biological capabilities that enhance their performance in biological systems. The in vitro evaluations demonstrated that this membrane can act as a repository for growth factors and stem cells. The in vivo bone repair facilitated by this membrane is notably effective, characterized by superior bone quality and accelerated formation rates. This process mirrors the natural intramembrane ossification, offering a promising approach to bone integration and regeneration.
研究领域:
组织工程
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再生医学
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骨生物学
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干细胞研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2017
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IF 7.504
Tianwen Xin,Yong Gu,Ruoyu Cheng,Jincheng Tang,Zhiyong Sun,Wenguo Cui,Liang Chen
Periosteum plays the pivotal role in neomineralization, vascularization and protection during bone tissue regeneration. However, many artificial periosteum focused only on protection and lacked of the osteogenesis and angiogenesis functional capacity. In this study, we developed a novelty inorganic strengthened gelatin hydrogel membrane via inorganic and organic co-cross-linked double network as artificial periosteum for enhancing the durable angiogenesis and osteogenesis in bone reconstruction. Mesoporous bioactive glass nanoparticles (MBGNs) chemically modified with photo-cross-linkable gelatin derivative (GelMA) were further incorporated into GelMA to fabricate an organic/inorganic co-cross-linked hydrogel membrane (GelMA-G-MBGNs). The GelMA-G-MBGNs hydrogel membrane displayed better mechanical property, durable degradation time, pH stable, biomineralization and long-term ion release. In vitro study demonstrated that, when compared with GelMA or GelMA/MBGNs, the GelMA-G-MBGN membrane significantly promoted osteogenic differentiation while maintaining stable local pH, which is conducive to cell adhesion and proliferation. Finally, the GelMA-G-MBGN membrane shows a superior artificial periosteum with superior capacity in angiogenesis and osteogenesis for accelerating new and mature lamellar bone formation in rat calvarial critical size defect. This co-cross-linked hydrogel membrane implied a promising strategy for the development of advanced periosteum biomaterials with excellent handle and bone repairing properties.
研究领域:
生物材料学
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组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
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2025
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IF 5.5
Yu Zhuang,Dingwei Wu,Lvyang Zhou,Boyuan Liu,Xingkai Zhao,Jianmin Yang,Wenge Liu,Zhenyu Wang,Yunquan Zheng,Xianai Shi
The inadequate vascularization and abnormal immune microenvironment in the diabetic bone defect region present a significant challenge to osteogenic regulation. Inspired by the distinctive characteristics of healing staged in diabetic bone defects and the structure–function relationship in the natural periosteum, we fabricated an electrospun bilayer biomimetic periosteum (Bilayer@E) to promote regeneration of diabetic bone defects. Here, the inner layer of biomimetic periosteum was fabricated using coaxial electrospinning fibers, with a shell incorporating zinc oxide nanoparticles (ZnO NPs) and a core containing silicon dioxide nanoparticles (SiO2 NPs) mimicking the cambium of periosteum; the outer layer consisted of randomly aligned electrospun fibers loaded with deferoxamine (DFO), simulating the fibrous layer of periosteum; finally, epigallocatechin-3-gallate (EGCG) was coated onto the bilayer membrane to obtain Bilayer@E. The presence of EGCG on the Bilayer@E surface efficiently triggers a phenotypic transition in macrophages, shifting them from an M1 proinflammatory state to an M2 anti-inflammatory state. Moreover, the sequential release of ZnO NPs, DFO, and SiO2 NPs exhibits antimicrobial characteristics while coordinating angiogenesis and promoting osteogenic mineralization in cells. Importantly, the biomimetic periosteum shows strong in vivo bone tissue and periosteal regeneration properties in diabetic rats. The integration of sequential drug release and immunomodulation, tailored to meet the specific healing requirements during bone regeneration, offers new insights for advancing the application of biomaterials in this field.
研究领域:
生物材料学
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再生医学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
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2018
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IF 3.057
Xun Liu,Yaxin Zheng,Yongjun Ma,Tingting Huo,Chonghua Pei
Using modified pig bone articular cartilage membrane as template, a sponge-like hydroxyapatite (HA) scaffold material was in-situ synthesized by self-assembly in simulated body fluid. Its crystallographic structure and composition were studied by SEM, XRD , FTIR and TEM, which showed that the synthesized sample was carbonated HA crystals and the three-dimensional interconnected sponge-like structure consists of massive nano hydroxyapatite plates. The physicochemical properties of the samples were tested and the results indicated that the sponge-like HA had high density, porosity and specific surface. Experimental cell culture results also showed that sponge-like HA greatly increases the bioactivity, osteoconductivity and differentiation of human osteosarcoma MG-63 cells.
研究领域:
生物材料学
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组织工程学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
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2022
·Vol. 12
·期 5
·2202008
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IF 11.092
Zeyang Liu,Haochen Nan,Yi Shiou Chiou,Zhen Zhan,Peter E. Lobie,Chengzhi Hu
Tissue-engineered periosteum substitutes (TEPSs) incorporating hierarchical architecture with osteoprogenitor and vascular niches are drawing much attention as a promising tool to support functional cells in defined zones and nourish the cortical bone. Current TEPSs usually lack technologies to closely observe cell performance, especially at the cell contact interface between distinct compartments containing defined biological configurations and functions. Here, an electrodeposition strategy is reported, which enables the selective formation of TEPSs with osteoprogenitor and vascular niches in a multiphasic scaffold in combination with different human cell types for cartilage regeneration in an in vivo osteochondral defect model. Human umbilical vein endothelial cells (HUVECs), dermal fibroblasts (HDFs), and bone marrow mesenchymal stem cells (hMSCs) are used to mirror both the vascular and osteogenic niches, respectively. It is observed that the intrinsic viscoelastic nature of the porous solid matrix is essential to successfully induce angiogenesis. Coculture of hMSCs with functional cells (HUVECs/HDFs) in TEPSs also effectively promoted periosteal regeneration, including osteogenic and angiogenic processes. The osteoarthritis cartilage histopathology assessment and histologic/histochemical grading system data indicate that the TEPSs containing hMSCs/HUVECs/HDFs exhibit superior potential for cartilage regeneration.
研究领域:
组织工程
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再生医学
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生物材料
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细胞生物学
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骨科
✓ 已验证
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
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2025
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IF 6.9
Shengdong Liu,Yilong Zheng,Qing Xiang,Zhufeng Yuan,Huanhuan Zhang,Yonggang Min
Periosteal repair is a sophisticated biological process involving the regeneration of bone tissue, blood vessels, and nerve complexes. However, current biomimetic periosteal materials are mainly insulating and only focus on osteogenesis and vascular regulation, often neglecting neural network reconstruction. In this study, we developed an electroactive sulfonated polyaniline/polypyrrole (SP/PPy) co-modified polycaprolactone (PCL) composite as a biomimetic periosteal membrane. Utilizing in-situ oxidative polymerization, SP was functionalized as a precursor layer, effectively addressing the issue of non-uniform PPy deposition on the hydrophobic polyester substrate. This modification enhanced the mechanical strength by 20.41 % and endowed the periosteal membrane a conductivity up to 220 S m −1 , enabling electrostimulation-mediated bidirectional differentiation for osteogenesis and neurogenesis. Experimental results demonstrated key advantages of this electroactive periosteum: 1. Superior Antibacterial Properties: The material achieved an inhibition rate of 98.56 % against Staphylococcus aureus . 2. Enhanced Biological Activity: The viability of rat bone marrow stromal cells (rBMSCs) reached 108.2 % of that of the blank sample. 3. Significant Electrostimulation Responsiveness: Under electrical stimulation, alkaline phosphatase (ALP) activity increased by 150.5 %, and the average axonal growth of PC-12 cells increased by 6.473 μm. This study provides a novel strategy for constructing electroactive multilayer biomimetic periosteal membranes, contributing to neuro-bone composite regeneration.
研究领域:
生物材料学
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组织工程
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再生医学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
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2025
·
IF 12.5
Wuyan Xu,Kun Liu,Jiaqing Chen,Junliang Liu,Siming Li,Hua Wang,Binghong Luo,Qingqi Meng
Bone regeneration is clinically challenging because vascularization, osteo-induction, and immune modulation are difficult to synchronize. Inspired by the native periosteum's bilayer architecture and multifunctional roles, we developed a wood-engineered Janus periosteum (EW-P-D@HACA-T) that integrates deferoxamine (DFO)-functionalized elastic wood (EW-P-D) with a bio-adhesive catechol-modified hyaluronic acid/tricalcium phosphate hydrogel (HACA-T). This construct mimics the anisotropic structure and compartmentalized functions of natural periosteum, providing dual support for angiogenesis and osteo-immunomodulation. EW-P-D maintains mechanical integrity and guides cell orientation, while sustained release activates the HIF-1α/VEGF pathway to enhance endothelial maturation and bone formation. Concurrently, the HACA-T composite hydrogel promotes osteogenic differentiation and polarizes macrophages toward an M2 phenotype, creating a pro-regenerative immune microenvironment. Furthermore, transcriptomic profiling revealed coordinated activation of p53, TGF-β, and ECM remodeling pathways, highlighting the scaffold's capacity to orchestrate tissue regeneration at the molecular level. In vivo evaluation using a rat skull defect model confirmed superior bone bridging, collagen deposition, neovascularization, and systemic biosafety. This study presents a structurally and functionally integrated wood-engineered periosteum substitute, demonstrating a paradigm shift toward engineered solutions that synchronize mechanical, angiogenic, and immunomodulatory cues for bone defect repair.
研究领域:
生物材料学
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组织工程
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免疫调控
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骨科学
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分子生物学
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转化医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
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2025
·
IF 8.1
Wen Zhuohao,Li Shuyi,Qiu Huiguo,Liu Xueyan,You Zhiying,Yan Yuhan,Che Yuejuan,Zhou Miao
Periosteum plays an indispensable role in bone regeneration by providing osteogenic and angiogenic cues essential for tissue repair. In cases of severe bone defects or nonunion, autologous vascularized periosteum transplantation remains a highly effective clinical solution. However, its application is restricted by donor site morbidity and limited tissue availability, thereby underscoring the urgent need for artificial periosteum that mimics both the composition and structure of the native counterpart. Among these properties, the topological morphology of the periosteum is believed to be critical, yet its influence on bone regeneration remains insufficiently understood. In this study, biomimetic periosteum membranes composed of coaxially electrospun poly(ε-caprolactone) (PCL) and periosteal extracellular matrix (pECM) were fabricated with either random or aligned nanofiber architectures. Their osteogenic potential was systematically evaluated in vitro and in vivo. Compared to the randomly arranged structure, aligned pECM (aPEC) significantly enhanced the adhesion, alignment, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by activating the ITGB1/PI3K/AKT signaling pathway, whereas these effects were not observed in pure PCL membranes. These findings demonstrate that aligned topological morphology in biomimetic periosteum plays a pivotal role in directing stem cell behavior and promoting bone regeneration. This work provides mechanistic insight and technical guidance for the future design of functionally enhanced artificial periosteum for bone tissue engineering applications.
研究领域:
组织工程
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生物材料学
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分子生物学
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再生医学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Advances
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2024
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IF 5.5
Hu Hui,Yuheng Song,Hongmei Liu,Jiahui Fan,Zhou Sha,Hongchuang Li,Jian Lu,Qian Zhang,Xiang Fei,Meifang Zhu
Artificial periosteum is deemed a novel strategy for inducing endogenous bone regeneration, but ideal periosteum substitutes achieved by orchestrating a biomimetic microenvironment for bone regeneration remain a significant challenge. Here, we design and fabricate a hybridized nanofiber-based artificial periosteum with boosted osteoinduction properties. Via a “molecular cage” biomineralization strategy, nano-hydroxyapatite (nano-HAp) with a controllable size (∼22 nm) and excellent dispersion serves as unique nano-additives for water-soluble polyvinyl-alcohol (PVA)-based artificial periosteum. The PVA/HAp composite is electrospun into nanofibers to replicate the extracellular-matrix-inspired nanostructure for inducing cell adhesion, proliferation, and fate manipulation. A simple post-crosslinking treatment is subsequently applied to further booster its mechanical strength (6.6 MPa) and swelling stability. The optimized sample of C-PVA/HAp (10 wt% nano-HAp) artificial periosteum features excellent biocompatibility and remarkable in vitro mineralization. Cell experiments demonstrate that its effectively boasted cell modulation for enhanced osteogenesis without the aid of growth factors, showing a possible activation of the ERK/MAPK signaling pathway. This work provides an effective strategy for designing novel HAp nano-additives and expands the possibility of biomimetic fabrication for more advanced nanofiber-based artificial periosteum.
研究领域:
生物材料
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组织工程
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骨再生
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2022
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IF 10.761
Qing Li,Wenbin Liu,Wen Hou,Xiaopei Wu,Wenying Wei,Jiawei Liu,Yihe Hu,Honglian Dai
The abundant neurovascular network in the periosteal fibrous layer is essential for regulating bone homeostasis and repairing bone defects. However, the majority of the current studies only focus on the structure or function, and most of them merely involve osteogenesis and angiogenesis, lacking an in-depth study of periosteal neurogenesis. In this study, a photothermal double-layer biomimetic periosteum with neurovascular coupling was proposed. The outer layer of biomimetic periosteum is a conventional electrospinning membrane to prevent soft tissue invasion, and the inner layer is an oriented nanofiber membrane to promote cell recruitment and angiogenesis. From the perspective of functional bionics, based on the whitlockite (WH) similar to bone composition, we doped Nd (the trivalent form of neodymium element) in it as the inducing element of photothermal response to prepare photothermal whitlockite ( [email protected] ). The sustained release of Mg 2+ in [email protected] can effectively promote the up-regulation of nerve growth factor (NGF) and vascular endothelial growth factor (VEGF). The release of Ca 2+ and PO 4 3− ions and photothermal osteogenesis jointly promote bone regeneration. Under the combined effect of structure and function, the formation of nerves, blood vessels, and related collagens greatly simulates the microenvironment of extracellular matrix and periosteum regeneration and ultimately promotes bone regeneration. In this study, physical and chemical characterization proved that the bionic periosteum has good flexibility and operability. The in vitro cell experiment and in vivo calvarial defect model verified that PPCL/ [email protected] biomimetic periosteum had excellent bone tissue regeneration function compared with other groups. Finally, PPCL/ [email protected] provides a new idea for the design of bionic periosteum.
研究领域:
生物医学工程
·
组织工程
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生物材料
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）