CRYSTENGCOMM
·
2024
·
IF 2.6
Yujing Ren,Xiubin Hou,Ying Zhang,Jingyan Hao,Juan Gao,Xinbing Song,Yurong Ma
Anhydrous guanine (AG) crystals are widely applied in organisms due to their excellent optical properties. However, the influence of biomolecules on the biomineralization process of guanine crystals in organisms remains unexplored. In this study, mixed proteins from the silver film of carp fish scales were extracted and were used as a model system to elucidate the effect of biomolecules on the guanine crystallization for the first time. The mixed proteins were enriched with amino acids such as Asp, Glu, Leu and Lys. Elongated hexagonal microplates of α-form AG (α-AG) mixed with a small amount of β-form AG (β-AG) mainly exposing (100) plane were obtained in the presence of the mixed proteins under mild condition while irregular particles were formed in the absence of proteins, indicating that the extracted mixed proteins have a strong influence on the formation of AG microplates exposing (100) plane. It is found that the proteins not only adsorb on the surface but also occluded into the AG crystals by using confocal laser scanning microscopy analysis. We also find that guanine analogues have profound synergetic effects on the morphology and polymorphs of guanine in the in-vitro synthetic system. The obtained α-AG exhibit superior optical properties such as high reflectivity, strong depolarization and pearlescent luster. This in-vitro synthesis work shows clearly the strong influence of biomolecules for the biomineralization process of guanine crystals in the organisms.
研究领域:
生物矿化
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材料科学
·
生物化学
·
光学
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DOI
高影响因子
BMC MICROBIOLOGY
·
2025
·Vol. 25
·期 1
·1-13
·
IF 4.2
Cao Xiaoyu,Zhang Yu,Xu Qianqian,Yan Hai
The overproduction of uric acid, driven by its key precursors (inosine and guanosine), leads to hyperuricemia, a metabolic disorder associated with severe complications such as gout and renal dysfunction. Here, a promising bacterial strain YC06 with excellent biodegradation capability for inosine and guanosine was successfully isolated from healthy individual feces and identified as Bacillus paranthracis through average nucleotide identity (ANI) analysis. B. paranthracis YC06 resting cells (live but suspended in PBS buffer) and its cell-free extracts could effectively biodegrade inosine and guanosine in vitro. Whole-genome sequencing revealed a 5,535,183 bp draft genome (52 contigs, 35.22% GC content) containing 5,672 protein-coding genes. B. paranthracis YC06 demonstrated high survival rates in simulated gastrointestinal fluids, supported by the presence of stress-response genes and bile salt hydrolase genes associated with gastrointestinal tolerance. However, this strain exhibited hemolytic activity and no amino acid decarboxylase activity, while hemolysin genes, antibiotic genes and toxin-producing genes were identified, raising potential biosafety concerns for its further application. The gene functional annotation and polymerase chain reaction (PCR) amplification electrophoresis identified key genes ( rihA , rihB , deoD and pnp ) encoding purine nucleosidase and purine-nucleoside phosphorylase, and combined with biodegradation product analysis, elucidated the pathways of inosine and guanosine biodegradation into hypoxanthine and guanine. Comprehensive safety evaluations, including cytotoxicity assay and in vivo pathogenicity studies, must be conducted to rigorously assess its risks prior to practical utilization.
研究领域:
微生物学
·
医学
·
基因组学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Hao Guo,Tingting Zhang,Mingyue Wang,Lei Sun,Junye Zhang,Meng Yang,Fan Yang,Ning Wu,Wu Yang
In this work, a novel sensor based on gold nanoparticle-modified zirconium-metal-organic frameworks (MOF-801) and carboxyl-functionalized carbon nanotube (MWCNT-COOH) composite material was built successfully for electrochemical detection of guanine and adenine in DNA sample, which exhibits diverse advantages such as fast, convenience and high efficiency. Guanine and adenine were detected by the cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Under the optimal conditions, MOF-801/MWCNT-COOH/ AuNPs/GCE delivered linear DPV responses to guanine in the range of 0.8 ~ 60, 60 ~ 180 μM and the adenine in the range of 0.8 ~ 60, 60 ~ 180 μM with the low detection limit of 0.09 μM for guanine and 0.08 μM for adenine, respectively. The advantages of this method include simplicity, rapidity, high sensitivity, good selectivity as well as low cost. The modified GCE was successfully applied to the analysis of guanine and adenine in DNA samples.
研究领域:
生物化学
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分析化学
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DOI
高影响因子
ANALYST
·
2012
·Vol. 137
·期 18
·4318-4326
·
IF 4.23
Peng Li,Wei Zhang,Jingjing Zhao,Fanjun Meng,Qiaoli Yue,Lei Wang,Haibo Li,Xiaohong Gu,Shuqiu Zhang,Jifeng Liu
An antioxidant (AO) amperometric technique based on guanine attached to graphene and Fe3O4 nanoparticles (NPs) magnetic materials was developed. Guanine molecules acted as an antioxidant competitor were bonded with graphene nanosheets, onto which magnetic Fe3O4 NPs were attached and the as-prepared magnetic composite can be attracted to the electrode surface by an external magnetic field. When applied with negative potentials, the dissolved oxygen was reduced to H2O2 at the electrode surface, and then reacted with the EDTA–FeII complex via a Fenton-like reaction to produce OH radicals. After oxidation damage by OH radicals, the electrochemical oxidation of guanine gave a decreased current. In the presence of AOs, the reactive oxygen species (ROS, e.g. OH radicals and H2O2) were scavenged by AOs and fewer guanine probe molecules were oxidized, thus inducing a higher electrochemical oxidation current of guanine. So AOs competed with the guanine probe molecules toward oxidation by ROS. The current signals of the guanine probe molecules were proportional to the concentrations of AOs. A kinetic model was proposed to quantify the ROS scavenging capacities of the AOs. Using guanine as an oxidizable probe and OH radicals and H2O2 as endogenous ROS, this kind of AO detection technique mimicks the antioxidant protection mechanism by small AO molecules in the human body.
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ELECTROCHEMISTRY COMMUNICATIONS
·
2019
·
IF 4.197
Jian Zhang,Dong Han,Shu Wang,Xiaodi Zhang,Ruiqi Yang,Yanchen Ji,Xin Yu
In this work, a WS 2 nanosheet/graphite microfiber hybrid electrode has been fabricated by in situ synthesis of WS 2 nanosheets on the surface of graphite microfibers. The WS 2 nanosheets possess an excellent electrocatalytic oxidation response towards adenine and guanine that encourages the adsorption of nucleobases and charge transfer at the surface of the microfibers. The WS 2 nanosheet/graphite microfiber hybrid electrode shows high sensitivity and selectivity for the detection of adenine and guanine. This hybrid microfiber electrode represents an excellent candidate platform for development of an electrochemical biosensor due to its low cost, flexibility and 3D morphology.
研究领域:
生物化学
·
材料科学
·
分析化学
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产品关联
DOI
高影响因子
JOURNAL OF DRUG TARGETING
·
2017
·
IF 3.068
Chang Liu,Sen Yao,Xuqian Li,Feng Wang,Yanyan Jiang
iRGD (internalizing RGD) with high affinity to αν integrins was reported to enhance tumor penetrability by binding to neuropilin-1 (NRP-1). Based on our previous study, chitosan surface-modified poly (lactide-co-glycolides) nanoparticles (PLGA/CS NPs), loaded with carmustine (BCNU) and its sensitizer (O6-benzylguanine, BG) showed stronger anti-tumor effect than free drugs. In present study, PLGA/CS NPs (NPs) with core-shell structure were prepared and modified with iRGD or mPEG. F98, C6 or U87 cell lines with different receptors levels were selected for in vitro and in vivo studies. After administration of iRGD-mediated NPs, including iRGD-modified NPs (iRGD-NPs) and co-administration of iRGD and NPs (iRGD + NPs), their effects on glioma were compared with NPs. iRGD-NPs showed stronger cytotoxicity and cellular uptake than other groups. iRGD-NPs and iRGD + NPs displayed deeper tumor penetration and stronger anti-invasion effect on three dimensional (3D) glioma spheroids than NPs. On F98 glioma-bearing mice model, iRGD-mediated NPs showed enhanced crossing BBB ability and brain tumor accumulation levels. Correspondingly, the median survival time of iRGD + NPs, iRGD-NPs and NPs groups were 58, 49 and 34.5 days, respectively. Present studies supported the iRGD-mediated strategy to improve the efficacy of antitumor drug delivery system. Importantly, co-administration of iRGD may be a greater way over the conjugation of iRGD.
研究领域:
药剂学
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肿瘤学
·
神经科学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Ruixue Yan,Hao Guo,Zeyun Yang,Wenting Niu,Jianpeng Ma,Liping Peng,Mingyue Wang,Wu Yang
Alterations in guanine (GA) and adenine (AE) levels within the human body are intricately linked to overall health and daily physiological functions. Therefore, it is crucial to develop an accurate and sensitive sensor for quantifying the levels of GA and AE. In this present paper, a novel electrochemical sensor NH 2 -MXene@COF TA-DH /GCE was constructed by in-situ growing COF TA-DH with high crystallinity, good thermal stability and N-rich active centers based on Schiff base condensation reaction of 1, 3, 5-tris-(4-aminophenyl) triazine (TAPT) and 2,5-dihydroxyl-terephthalaldehyde (DHTA) on highly conductive NH 2 -MXene and then modifying it on the surface of the glass carbon electrode (GCE). The highly dispersed sandwich structure of NH 2 -MXene@COF TA-DH significantly increases the specific surface area and exposes a greater number of active sites. Benefitted from remarkable synergistic effect, the sensor exhibited excellent electrochemical catalytic activity and high sensitivity for GA and AE detection. The sensing platform was successfully used for simultaneous detection of GA and AE with wide linear ranges of 0.6–230 μM and 1–450 μM and low detection limits of 0.07 μM and 0.44 μM, respectively. This study expands applications of COFs and MXene in electrochemical biosensing platform and provides a general and valuable strategy for COFs-based electrochemical sensor construction for biomedical investigations
研究领域:
生物医学
·
分析化学
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产品关联
DOI
高影响因子
Materials Today Communications
·
2024
·
IF 3.7
Yicheng Zhou,Li Dong,Jiazheng Zhang,Rongrong Qiao,Lin Liu,Chao Liu,Xiaobo Zhang,Zhiwei Tong
In this study, a nanocomposite of Polyaniline/titanium carbide (PANI/Ti 3 C 2 ) was synthesized through self-assembly and in situ polymerization for use as a sensor material to simultaneously detect guanine (G), adenine (A), and uric acid (UA). X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) were utilized to confirm the successful synthesis of PANI/Ti 3 C 2 . Electrochemical analysis methods including differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) were used to detect the electrochemical performance of PANI/Ti 3 C 2 modified glassy carbon electrode (PANI/Ti 3 C 2 /GCE). The pH dependence, electrochemically active surface area (ECSA), electron transport , stability, reproducibility, sensitivity, and selectivity of the modified GCE were examined. Compared to previous reports, the nanocomposite exhibited excellent performance. Uric acid, guanine, and adenine detection limits were 0.33, 0.31, and 0.28 μM, respectively, over concentration ranges of 0.62 - 20.10, 0.31 - 17.00, and 0.31 - 17.00 μM. The nanocomposite demonstrated high recovery and low error in serum sample detection. In summary, PANI/Ti 3 C 2 is a sensor material with excellent electrochemical properties .
研究领域:
材料科学
·
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Zai-yong Li,Hong-jiang Quan,Cheng-bin Gong,Yu-zhu Yang,Qian Tang,Yu-bo Wei,Xue-bing Ma,Hon-wah Lam
A novel photoresponsive molecularly imprinted polymer (MIP) was developed for the selective extraction of guanine from complex samples. The photoresponsive MIP was fabricated using guanine as the template, water-soluble 5-[(4-(methacryloyloxy)phenyl)diazenyl]isophthalic acid as the functional monomer, and water-soluble triethanolamine trimethacrylate as the cross-linker. The MIP displayed good selectivity toward guanine with a dissociation constant of (2.70 ± 0.16) × 10 −5 mol L −1 in aqueous media. The density of the guanine-specific receptor sites in the MIP material was (4.49 ± 0.22) μmol g −1 . Quantitatively release and uptake of guanine by the MIP occured with irradiation at 365 and 440 nm, respectively. The MIP could efficiently extract guanine from beer and then release it into aqueous media under photocontrol. This method could be used for selective separation and subsequent determination of a specific analytes from complex samples.
研究领域:
分析化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2015
·Vol. 162
·期 12
·B332
·
IF 3.266
Joanna Borowiec,Jingdong Zhang
In this study, boron-doped graphene (B-G) nanosheets (Ns) were synthesized using NaBH4 as both reductant and source of boron (B) dopant atoms during hydrothermal treatment of graphene oxide (GO), in the presence of poly(diallyldimethylammonium chloride) (PDDA). The as-prepared material was characterized by several analysis techniques, such as scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD) and voltammetry. The electrochemical performance of the synthesized PDDA functionalized B-G nanocomposite-modified glass carbon electrode (GCE) toward guanine was investigated. The results show, that the incorporation of B atoms into the structure of G significantly improved its electrocatalytic activity toward guanine oxidation. The PDDA/B-G based electrochemical sensor exhibits linear relationship between guanine concentration in the range from 1 × 10−6 to 7.5 × 10−5 mol L−1, with detection limit of 3.9 × 10−7 mol L−1. This approach of using B-doped graphene provides a feasible approach for fabrication of electrochemical sensor.
研究领域:
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2012
·
IF 2.905
Xiao Liu,Liqiang Luo,Yaping Ding,Qingsheng Wu,Youli Wei,Daixin Ye
In this study, a polymerized film of melamine (PMel) was first prepared on the surface of glassy carbon electrode (GCE) in acidic solution by cyclic voltammetry. Scanning electron microscopy, Fourier transforms infrared spectroscopy and electrochemical techniques were used for characterization of the modified electrode surface. The electrochemical activity of the poly-melamine film modified glassy carbon electrode (PMel/GCE) was primarily tested for electrochemical catalyzes of guanine (G), adenine (A) and epinephrine (EP) in 0.1 M phosphate buffer solution (pH 4.5). The oxidation currents of G, A, and EP at the PMel/GCE increased 9, 7 and 13 times respectively compared with that of bare GCE. In the detection of the three aforementioned analytes using square wave voltammetry, the linear ranges of the peak currents at the PMel/GCE were 0.1–50 μM G, 0.1–60 μM A and 0.1–100 μM EP with detection limits of 0.08 μM G, 0.07 μM A and 0.05 μM EP, respectively. Furthermore, the prepared electrode displayed voltammetric responses with high sensitivity, good selectivity and reproducibility for A, G, and EP in optimal conditions, making it very suitable for determination of the three components.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2017
·Vol. 21
·期 5
·1357-1364
·
IF 2.316
Chen Yalin,Mei Tao,Chen Yi,Wang Jianying,Li Jinhua,Wang Xianbao
The nanocomposite graphene oxide/8-aminoquinoline (GAQ) was prepared as modified electrode material for simultaneous determination of guanine and adenine. The prepared GAQ was characterized by SEM, TEM, FTIR, Raman, and UV-vis, which confirmed that the 8-aminoquinoline had been functionalized by covalent modification. The differential pulse voltammetry (DPV) proved the electrochemical properties of the GAQ, which exhibited good electrocatalytic activity and prominent synergistic effect for sensitive determination of guanine and adenine. The guanine and adenine both were detection by DPV showed good linearity with linear range covering 0.1–160 μM, and the detection limits (LOD) ( S/N = 3) both were estimated to be 0.033 μM for guanine and adenine, respectively.
研究领域:
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）