EUROPEAN FOOD RESEARCH AND TECHNOLOGY
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2021
·Vol. 248
·期 2
·553-565
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IF 2.998
Wang Liling,Qin Yuchuan,Wang Yanbin,Liu Bentong,Zhou Yifeng
In this research, the inhibition mechanism of a pairs of homoisoflavonoid analogues (6-aldehydo-isoophiopogonanone A (AIO-A) and 6-aldehydo-isoophiopogonanone B (AIO-B)) on tyrosinase (Tyr) was investigated by multi-spectroscopic techniques combined with molecular docking. The results demonstrated that AIO-A suppressed the activity of Tyr in a reversible competitive-inhibition with a half inhibitory concentration (IC50) of (12.67 ± 2.52) × 10 –5 molˑL −1 , while AIO-B was a reversible mixed-inhibition with IC50 of (4.81 ± 0.74) × 10 –5 molˑL −1 . AIO-A/AIO-B bound to Tyr driven by the intermolecular interactions, including van der Waals interaction, hydrogen bonding and hydrophobic interaction, thereby resulted in a secondary structure change of Tyr and its intrinsic fluorescence quenching. The molecular docking revealed that an aldehyde oxygen atom in AIO-A and methoxyphenyl in AIO-B formed coordination with two Cu ions in the active center of Tyr protein. It can be deduced that the coordination with two Cu ions may prevent the entrance of substrate and then inhibit the catalytic activity of Tyr. The research might provide new perspectives on the inhibition mechanism of AIO-A and AIO-B on Tyr and functional research for the prevention and treatment of pigmented skin diseases and anti-browning of homoisoflavonoids as a food supplement.
研究领域:
生物化学
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药理学
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植物化学
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DOI
高影响因子
Frontiers in Pharmacology
·
2021
·
IF 5.811
Yu Kunzi,Liu Wei,Zhang Nanping,Cheng Xianlong,Zhou Shiyu,Zuo Tiantian,Kang Shuai,Wei Feng,Ma Shuangcheng
Maidong, the root tuber of Ophiopogon japonicus (Thunb.) Ker Gawl., is a commonly used herbal medicine in China. There are three quality grades of Maidong according to traditional opinion and modern research studies: superior quality (Zhe-Maidong), medium quality (Chuan-Maidong), and poorest quality (Chuan-Maidong with paclobutrazol, which is a kind of plant growth regulator). However, no efficient way to distinguish the three quality grades of Maidong exists; thus, the herbal markets and botanical pharmacies are flooded with Chuan-Maidong with paclobutrazol. To ensure the safety and quality of Maidong, a comparative microscopic study was performed on three quality grades of Maidong. The result was to establish a microscopic quantification method based on the area ratio between xylem and pith to distinguish the three quality grades of Maidong. Subsequently, Maidong from regional markets was evaluated by this method. In this study, we developed a novel quantification method to identify the three quality grades of Maidong, which could in turn make efforts on the quality improvement of Maidong. Our study is the first to demonstrate that microscopic technology could be used to distinguish different quality grades of a specific herbal medicine.
研究领域:
中药学
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植物显微学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2010
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IF 2.469
Hui Wang,Jin Qi,Tian Xu,Ji-Hua Liu,Min-Jian Qin,Dan-Ni Zhu,Bo-Yang Yu
Fungal invasion is a main factor leading to the loss of postharvest herbs during storage. To prevent fungal invasion and clarify the favorable conditions for the stability of herbs during the storage period, uniform design and three-dimensional response surfaces were applied to investigate the relationship of the mildew degree of Radix Ophiopogonis induced by prevalent fungal isolates and the storage factors including air relative humidity (X1), temperature (X2), and moisture content (X3), in laboratory studies. Mildew degree was evaluated by ergosterol assay of mold isolates through a high-performance liquid chromatography−diode array detector (HPLC-DAD) technique. As a result, storage conditions, which satisfied the following correlation equations established by the relationships between the storage factors and the biomass of mildew (Y), could effectively prevent fungal spoilage: Y = −0.2484084028 + 0.00030711966572 × X3 × X3 + 0.00018881361186 × X1 × X2 − 0.00029473040679 × X2 × X3 ≤ 0; Y = −0.2697457586 + 0.004127756022 × X1 − 0.000015977780492 × X1 × X1 + 0.00021906984606 × X2 × X3 ≤ 0; Y = −0.325655811 + 0.015464432582 × X2 + 0.00004779394354 × X1 × X1 − 0.00021743815482 × X2 × X2 ≤ 0. This compositive methodology might be useful to predict the occurrence of fungal invasion.
研究领域:
中药学
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植物病理学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 21
·7380
·
IF 4.927
Yaoyao Zhu,Liling Wang,Meixu Chen,Yifeng Zhou,Jun Huang
ZhejiangOphiopogonjaponicus(ZOJ) is a specific variety ofOphiopogon japonicuswith characteristic steroidal saponins and homoisoflavonoids, which are also main pharmacodynamic constituents with clinical effects, including curing inflammation and cardiovascular diseases. However, few analysis methods were applied to simultaneously and quantitatively determine two kinds of its constituents, and hazardous organic solvents are mostly used for extraction. In this study, a new validated simultaneous extraction and determination method for four characteristic steroidal saponins and homoisoflavonoids in ZOJ was established by ionic liquid–ultrasonic extraction (IL-UAE) combined with HPLC-DAD-ELSD analysis, which can be used for the quality control of ZOJ. Chromatographic separation was performed with a DAD wavelength at 296 nm, and the ELSD parameters of the drift tube temperature (DTT), atomizer temperature (AT), and nitrogen gas pressure (NGP) were set at 20% heating power, 70 °C, and 25 psi, respectively. The optimal IL-UAE conditions were 1 mol/L [Bmim]CF3SO3aqueous solution, a liquid–material ratio of 40 mL/g, and an ultrasonic time of 60 min. The proposed method is reliable, reproducible, and accurate, which were verified with real sample assays. Consequently, this work will be helpful for the quality control of ZOJ. It can also present a promising reference for the simultaneous extraction and determination of different kinds of constituents in other medicinal plants.Keywords:ionic liquid (IL);ZhejiangOphiopogonjaponicus(ZOJ);steroidal saponins;homoisoflavonoids;HPLC-DAD-ELSD
研究领域:
药用植物学
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分析化学
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药用植物
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NATURAL PRODUCTS
·
2025
·
IF 3.6
Xijian Wu,Zijie Su,Biling Huang,Xin Peng,Xing Zhang,Shaohua Huang
The detection and analysis of chiral molecules have long been challenging in analytical chemistry. This study introduces a novel approach that utilizes 2-trifluoromethyl benzaldehyde as a small-molecule probe capable of forming a stable Schiff base with chiral amino acids in aqueous solution under alkaline conditions. The amino acid Schiff bases present a strong Cotton effect and UV absorption at wavelengths exceeding 260 nm, enabling chiral analysis, including assignment of absolute configuration, enantiomeric composition, and total concentration. An application of this method was the authentication of the herbal medicine Ophiopogon japonicus. Using principal component analysis and orthogonal partial least squares discriminant analysis, we successfully differentiated O. japonicus samples collected in two distinct locations with 20 samples. This rapid and convenient method offers a new approach to quality control of herbal medicine.
研究领域:
分析化学
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中药学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
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IF 6.7
Xijian Wu,Xin Tong,Biling Huang,Shaohua Huang
Rapid detection of amines in complex mixtures presents a significant challenge. Here, we introduce a novel nuclear magnetic resonance (NMR) method for amine detection utilizing a probe with two fluorine atoms in distinct chemical environments. Upon interaction with an amine, the probe generates two atomic resonance peaks, which are used to create coordinates, revealing fluorine chemical shifts on the 19F NMR spectroscopy. This innovative approach allows for the clear distinction of amine signals in a two-dimensional plane. This method has been effectively employed in analyzing amines in pharmaceuticals and amino acids in Ophiopogon japonicus and dry white wine, providing a robust and general approach for amine analysis.
研究领域:
分析化学
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药物分析
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核磁共振波谱学
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2024
·Vol. 39
·期 1
·e6050
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IF 1.8
Tiantian Xie,Yanling Hao,Yanzheng Xie
Methylophiopogonanone A (MOA) is one of the homoisoflavonoids isolated from Ophiopogon japonicus , which has been demonstrated to have extensive pharmacological activities. The aim of this study was to develop and validate a liquid chromatography–tandem mass spectrometry method for the measurement of MOA in rat plasma. Methylophiopogonanone B (MOB) was used as internal standard. After precipitation with acetonitrile, the samples were separated using a Waters ACQUITY HSS T 3 column with 0.1% formic acid solution and acetonitrile as mobile phase. Mass detection was monitored using multiple reactions monitoring mode with precursor-to-product ion transition of m/z 343.2 > 135.1 for MOA and m/z 329.2 > 121.1 for internal standard. The assay showed good linearity over the concentration range of 1–1000 ng/mL, with correlation coefficient > 0.997. The lower limit of quantification (LLOQ) was 1 ng/mL. Acetonitrile-mediated precipitation showed high extraction efficiency (> 80%). The accuracy and precision were within the acceptable limits. MOA was demonstrated to be stable in rat plasma under the tested storage conditions. After validation, the proposed method was applied to the pharmacokinetic study of MOA in rat plasma, and the data revealed that MOA showed rapid absorption and elimination from rat plasma. The oral bioavailability of MOA in rat was 24.5%.
研究领域:
药物分析
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药理学
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产品关联
DOI
PubMed
高影响因子
STARCH-STARKE
·
2023
·
IF 2.3
Yao Wen,Caimei Su
A Box–Behnken design (BBD) of response surface methodology (RSM) is employed to further optimize ultrasonic-assisted extraction conditions for Ophiopogon japonicus polysaccharides. The results show that optimized extraction conditions are liquid–solid ratio of 22:1 mL g −1 , ultrasonic time of 27 min, and ultrasonic power of 200 W. Under these conditions, the mean experimental value of extraction yield (39.49%) is achieved, which is 1.33 times that of distilled water leaching extraction (29.68%). In addition, one of the eluting fractions, SPs-1 , has high α -glucosidase and α -amylase inhibitory activities as well as 1,1-Diphenyl-2-picrylhdrazyl radical and α-glucosidase, 2,2’-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) cation scavenging activities with an IC 50 value of 0.060, 0.021, 0.50, and 0.10 mg mL −1 . Therefore, SPs-1 from O. japonicus can be considered as multifunctional biological ingredients to be used in anti-hyperglycemic pharmaceutical formulation and antioxidant in functional foods.
研究领域:
药学
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食品科学
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生物化学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
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IF 4.1
Yujie Guo,Puyang Gong,Yin Qian,Haichun Liu,Boyang Yu,Jin Qi
In the present study, a comprehensive strategy integrating affinity ultrafiltration high-performance liquid chromatography quadrupole-time-of-flight mass spectrometry (UF-HPLC-Q-TOF-MS), in silico molecular docking and bioassays was established to rapidly screen natural SOD activators from traditional Chinese medicines. As illustrative case studies, Schisandra chinensis, Fructus cnidii and Radix ophiopogonis were chosen to develop and verify the strategy. The HPLC-Q-TOF-MS was used to identify the compounds in comparison with reference standards and literature data. A total of eight compounds, including four biphenyl-cyclooctene ligands from Schisandra chinensis and four coumarins from Fructus cnidii, were found to potentially increase SOD activities. No ligands were found in the extract of Radix ophiopogonis. Then, in silico molecular docking was performed to investigate the binding site and binding affinity of the candidates on SOD. Compared to the nonspecific ligands screened from the extract, the specific ligands presented stronger binding affinities. In addition, the activity and kinetic parameters of the SOD-ligand were investigated through an improved pyrogallol autoxidation method. Gomisin J and xanthotoxin showed a stronger ability to increase SOD activities. The present study indicated that combining UF-HPLC-Q-TOF-MS and in silico molecular docking offers a powerful and meaningful tool to rapidly screen SOD activators from traditional Chinese medicines.
研究领域:
药理学
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分析化学
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生物信息学
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中医药
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Yujie Guo,Renjie Fu,Yin Qian,Zhenzhen Zhou,Haichun Liu,Jin Qi,Boli Zhang,Boyang Yu
Off-line multi-hyphenated methods including affinity-ultrafiltration mass spectrometry, molecular docking, and reverse-phase high-performance liquid chromatography-fluorescence detection (RP-HPLC-FD) were used to rapidly screen and identify natural inducible nitric oxide synthase (iNOS) inhibitors isolated from Ophiopogon japonicus (Thunb.) Ker-Gawl. Ultrafiltration high-performance liquid chromatography quadrupole time-of-flight mass spectrometry was used to screen and identify the potential ligands with high binding affinity to iNOS. Seven compounds (three saponins and four flavonoids) were found in Radix Ophiopogonis. Molecular docking identified the binding sites and found that the binding affinity of the three ophiopogonis ligands was higher than that of the four homoisoflavonoids. Pre-column derivatization of l -citrulline and RP-HPLC-FD verified that the in vitro inhibition activity of the three ophiopogonis compounds was stronger than that of the four homoisoflavonoids, which was similar to the screened results. In conclusion, combining an in vitro iNOS inhibition assay and affinity-ultrafiltration mass spectrometry with molecular docking was a powerful tool for rapid screening and identification of iNOS inhibitors isolated from this traditional Chinese medicine.
研究领域:
药理学
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分析化学
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中医药学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2022
·
IF 5.195
Jinxia Hou,Lanping Zhong,Jianming Liu,Fanglan Liu,Chunhua Xia
Ethnopharmacological relevance Shengmai formula (SMF) is a classical traditional Chinese medicine prescription, which is widely used in the treatment of cardiovascular and cerebrovascular diseases . Our previous studies have demonstrated that some components in SMF can interact with each other through breast cancer resistance protein , sodium taurocholate co-transporting polypeptide , organic anion transporting polypeptide 1B1 and 1B3. Organic anion transporter 1 (OAT1) is highly expressed in kidney, mediating the elimination of many endogenous and exogenous substances. However, the interaction between the main active components in SMF and OAT1 is not clear. Aim of the study This study aimed to investigate the interactions of the major bioactive components in SMF mediated by OAT1. Materials and methods Four main fractions, namely, ginseng total saponins (GTS), ophiopogon total saponins (OTS), ophiopogon total flavonoids (OTF), fructus schisandrae total lignans (STL), and 12 active components, namely, ginsenoside Rg1, Re, Rd and Rb1, ophiopogonin D and Dˊ, methylophiopogonanone A and B, schizandrol A and B, schizandrin A and B, were selected to explore the interactions of SMF with OAT1 using cell and rat models. Results The above four main fractions in SMF all exhibited inhibitory effects on the uptake of 6-carboxyfluorescein (6-CF), a classic substrate of OAT1. Among the 12 main effective components, only ginsenoside Re , Rd, and methylophiopogonanone A showed inhibition of 6-CF uptake. Additionally, we found that schizandrin B was transported by HEK293-OAT1 cells, and schizandrin B uptake was markedly inhibited by GTS, OTS, OTF, ginsenoside Re, Rd, and methylophiopogonanone A. In rats, ginsenoside Re, Rd, and methylophiopogonanone A jointly increased the AUC (0-t) , AUC (0-∞) , and C max of schizandrin B, but they decreased its clearance in plasma and excretion in urine. Conclusions Ginsenoside Re, Rd, and methylophiopogonanone A were the potential inhibitors of OAT1, and may interact with some drugs serving as OAT1 substrates clinically. Schizandrin B was a potential OAT1 substrate, and its OAT1-mediated transport was inhibited by ginsenoside Re, Rd, and methylophiopogonanone A. OAT1-mediated interactions of the main active components in SMF can be regarded as one of the important compatibility mechanisms of traditional Chinese medicine preparations.
研究领域:
药理学
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中药学
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分子生物学
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药物代谢
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产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2022
·
IF 6.656
Zhonghao Li,Qinmei Sun,Qingyang Liu,Xinru Mu,Hui Wang,Han Zhang,Fenfen Qin,Qisheng Wang,Dengyun Nie,Anlong Liu,Qian Li,Jianjian Ji,Yongwei Jiang,Shengfeng Lu,Qian Wang,Zhigang Lu
Background Opioids are widely used in clinical practice. However, their long-term administration causes respiratory depression , addiction, tolerance, and severe immunosuppression . Traditional Chinese medicine (TCM) can alleviate opioid-induced adverse effects. Compound 511 is particularly developed for treating opioid addiction, based on Jiumi Liangfang, an ancient Chinese drug treatment and rehabilitation monograph completed in 1833 A.D. It is an herbal formula containing eight plants, each of them contributing to the overall pharmacological effect of the product: Panax ginseng C. A. Meyer (8.8%), Astragalus membranaceus (Fisch.) (18.2%), Datura metel Linn. (10.95%), Corydalis yanhusuo W. T. Wang (14.6%), Acanthopanar gracilistμlus W. W. Smith (10.95%), Ophiopogon japonicus (Linn. f.) Ker-Gawl. (10.95%), Gynostemma pentaphyllum (Thunb.) Makino (10.95%), Polygala arvensis Willd. (14.6%). This formula effectively ameliorates opioid-induced immunosuppression. However, the underlying mechanism remains unclear. Purpose To reveal the effects of Compound 511 on the immune response of morphine-induced immunosuppressive mice and their potential underlying molecular mechanism. This study provides information for a better clinical approach and scientific use of opioids. Methods Immunosuppression was induced in mice by repeated morphine administration. Th1/Th2/Th17/Treg cell levels were measured using flow cytometry. Splenic transcription factors of Th1/Th2/Th17/Treg and outputs of the regulatory PI3K/AKT/mTOR signaling pathway were determined. Subsequently, methicillin-resistant Staphylococcus aureus (MRSA) was administered intranasally to morphine-induced immunosuppressive mice pretreated with Compound 511. Their lung inflammatory status was assessed using micro-computer tomography (CT), hematoxylin and eosin (H&E) staining, and enzyme-linked immunosorbent assay (ELISA). Results Compared to morphine, Compound 511 significantly decreased the immune organ indexes of mice, corrected the Th1/Th2 and Treg/Th17 imbalance in the immune organs and peripheral blood, reduced the mRNA levels of FOXP3 and GATA3 , and increased those of STAT3 and T-bet in the spleen . It improved immune function and reduced MRSA-induced lung inflammation. Conclusion Compound 511 ameliorates opioid-induced immunosuppression by regulating the balance of Th1/Th2 and Th17/Treg via PI3K/AKT/mTOR signaling pathway. Thus, it effectively reduces susceptibility of morphine-induced immunosuppressive mice to MRSA infection .
研究领域:
药理学
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免疫学
·
微生物学
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中药学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）