EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2025
·
IF 4.7
Ming Xu,Wei Wang,Jiayan Wang,Di Wu
Chronic rhinosinusitis (CRS), a common chronic sinonasal inflammatory disease, frequently requires surgical intervention, yet postoperative bleeding and adhesions remain challenging. While topical hyaluronic acid (HA) and corticosteroids show therapeutic potential, conventional formulations often suffer from rapid drug release. This study developed a pH-responsive HA hydrogel (HA@F127-MF) loaded with mometasone furoate (MF) to address these limitations. Oxidized HA (OHA) and aminated HA (HA-ADH) were synthesized and verified via FTIR and ¹H NMR. The hydrogel demonstrated exceptional swelling capacity, achieving ∼1500% equilibrium swelling ratio in PBS within 8 hours. Rheological tests revealed rapid gelation, self-healing properties, and favorable viscoelasticity, while SEM confirmed its stable 3D microporous structure. Sustained MF release (79.4% over 14 days) was achieved with pH-dependent kinetics. In vitro cytotoxicity assays using human nasal epithelial cells (HNEPCs) indicated excellent biocompatibility. APTT and PT tests suggested enhanced hemostatic effects via the intrinsic coagulation pathway. These findings position HA@F127-MF hydrogel as a promising dual-functional platform for CRS postoperative care, combining sustained anti-inflammatory drug delivery with hemorrhage control. However, further in vivo validation and clinical trials are warranted to confirm its efficacy and safety for translational applications. This dynamic drug-release hydrogel design may enhance clinical outcomes by concurrently addressing postoperative bleeding and chronic inflammation.
研究领域:
医学材料学
·
药剂学
·
耳鼻喉科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CLINICAL AND EXPERIMENTAL ALLERGY
·
2025
·
IF 5.2
Yanran Huang,Zhaofeng Xu,Yan Yan,Chunbo Li,Jieying Yan,Yi Wei,Jian Li,Luo Zhang,Nan Zhang,Claus Bachert,Weiping Wen
Background Immunoglobulin overproduction was observed in type 2 chronic rhinosinusitis with nasal polyps (CRSwNP). Endoplasmic reticulum (ER) stress is linked to aggregation in various inflammatory diseases, yet its presence and extent in nasal polyps remain to be elucidated. Objective To assess the impact of B-cell ER stress on local immunoglobulin production in CRSwNP and investigate its correlation with disease severity. Methods Single-cell mapped transcriptional profiles at cellular resolution. Electron microscopy revealed ultrastructural features, complemented by immunohistochemistry/immunofluorescence mapping marker localisation. Western blotting quantified protein expression, with QuantiGene Plex and Luminex enabling multiplex cytokine analysis. Bulk RNA sequencing and targeted protein expression validation in ex vivo experiments confirmed critical findings. Results HSPA5 and HSP90B1 were found to be two major elevated ER stress markers in type 2 CRSwNP, compared to the control nasal tissue, and their expression correlated with the expression of IGHE and type 2 inflammatory markers. In CRSwNP, the ER stress signature score and increased marker expression predominantly originated from B and plasma cells. Electron microscopy revealed dilated ER and enlarged lumen in sorted B cells from nasal polyps. MZB1 exhibited co-localisation with plasma cells and mature B cells. Immunofluorescence staining demonstrated that MZB1 co-localised with HSPA5 and HSP90B1. In vitro, stimulation with MZB1 upregulated mRNA expression of ER stress markers and IgE. Increased IGHE expression was detected in response to ER stress induced in vitro. Finally, anti-IgE treatment inhibited the expression of ER stress-related genes. Conclusion ER stress markers were significantly upregulated in CRSwNP. Specifically, ER stress levels were significantly elevated in B cells of CRSwNP compared to controls. In type 2 CRSwNP, B-cell ER stress may play a role in promoting local IgE production. Graphical The ER stress burden was significantly upregulated in type 2 CRSwNP tissues compared to both healthy controls and non-type 2 polyp tissues. This elevated stress was linked to more severe type 2 inflammation and increased local IgE production. In vitro studies of B cells derived from nasal polyp tissues demonstrated that MZB1 upregulated endoplasmic reticulum stress, which was associated with enhanced IgE level. Furthermore, in vivo treatment with Omalizumab reduced endoplasmic reticulum stress levels. ER, endoplasmic reticulum; HC, healthy controls; CRSwNP, chronic rhinosinusitis with nasal polyps; MZB1, Marginal Zone B1 protein.
研究领域:
耳鼻喉科学
·
免疫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
American Journal of Translational Research
·
2025
·Vol. 17
·期 7
·5475
·
IF 1.6
Bin Shi,Wen Yu,Bei Qi,Huifang Liu,Rui Jiang
Objective: To evaluate the efficacy of endoscopic minimally invasive surgery (EMIS) in improving olfactory function and quality of life among patients with chronic rhinosinusitis with nasal polyps (CRSwNP). Methods: A cohort of 117 patients diagnosed with CRSwNP between January 2020 and June 2024 were included in this retrospective study. Patients were assigned to either a control group (n=50, treated with conventional endoscopic sinus surgery [ESS]) or a research group (n=67, treated with EMIS). Comprehensive assessments were conducted, including treatment efficacy, safety profile, olfactory function, nasal ventilation (airway resistance), nasal status, stress biomarkers, and quality of life. Results: Compared to the control group, the research group showed significantly better outcomes in terms of: (1) overall therapeutic efficacy, (2) postoperative safety profile (lower postoperative complication rates), (3) olfactory function recovery, (4) nasal ventilation and functional status, (5) quality of life improvement, and (6) reduced surgical stress response (all P<0.05). Conclusion: EMIS significantly improves olfactory function and quality of life in patients with CRSwNP, offering clinical advantages over conventional ESS and supporting its broader clinical application.
研究领域:
耳鼻喉科学
·
神经外科
·
临床医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2021
·
IF 6.419
Tao Liu,Guowei Li,Xidong Wu,Shaohua Chen,Siyi Zhang,Hong Han,Hongbin Zhang,Xiaoning Luo,Xiang Cai,Dong Ma
Chronic rhinosinusitis (CRS) is a rather prevalent condition with a chronic inflammatory process, which is hard to cure. Herein, a new antibacterial drug, nitric oxide (NO), was used for the attempt on CRS therapy. To achieve this, a star copolymer (β-CD-PAMAM) consisting of the β-cyclodextrin (β-CD) core and seven PAMAM-G3 arms, which was designed as a low-cytotoxicity and high NO loading carrier, were synthesized and characterizied. The obtained β-CD-PAMAM/NONOate showed the effect in inhibiting and dispersing the biofilm of S. aureus, as well as the effective antibacterial performance, implying the promising application in CRS treatment. The in vivo assay confirmed that β-CD-PAMAM/NONOate displayed excellent therapy effect on CRS and significantly improved the symptoms of the experimental rats, which was no significant different in therapy effect with the clinical Rhinocort. Incorporated with its little toxicity in vitro and in vivo, the β-CD-PAMAM/NONOate was suggested a promising application in CRS therapy.
研究领域:
药学
·
医学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Zhen-Cai Xu,Yu-Feng Ge,Kai-Yong Chen,Min-Hua Liu
Sulfur dioxide (SO 2 ) plays an extremely important role in the basic processes of physiology and pathology. As an antioxidant , SO 2 can maintain the redox homeostasis in the cell. Excessive inhalation of SO 2 would lead to irreparable respiratory damage, resulting in respiratory diseases , neurological disorders , and even cardiovascular disease. Thus, it is urgent to exploit an excellent way to monitor SO 2 derivatives in biological system. Herein, we design a water-soluble ratiometric fluorescent probe to fast detect the level of SO 2 derivatives in living cells in vivo. The probe displays obvious fluorescence signal at long wavelength, which is helpful for imaging of biological system. After respond to SO 2 derivatives, the fluorescence signal at 465 nm increases rapidly due to the Michael addition reaction is triggered, further causing the disruption of large conjugated system . The probe exhibits high selectivity and fast respond to SO 2 derivatives, which can be able to sensitive and real-time monitoring of SO 2 derivatives level in living cells. Moreover, the probe reveals a low detection limit and a great linear relationship to SO 2 derivatives. Based on the negligible cytotoxicity and good biocompatibility of the probe, which is employed to detect exogenous and endogenous SO 2 derivatives in living cells. In addition, it is also served as a potential chemical tool to detect SO 2 derivatives in mice model of sinusitis .
研究领域:
生物化学
·
医学诊断
·
分子影像学
✓ 已验证
DOI
高影响因子
Clinical and Experimental Otorhinolaryngology
·
2023
·
IF 3
Kunyu Liu,Yu Xu
Objectives. Hypoxia-inducible factor 1α (HIF1α) and Tet methylcytosine dioxygenase 2 (TET2) have been reported to mediate nasal polypogenesis through the epithelial-to-mesenchymal transition (EMT). Additionally, HIF1α can regulate the expression and function of TET2. However, the precise mechanism of how TET2 regulates the EMT through HIF1α mediation in nasal epithelial cells is still poorly understood. Methods. Nasal tissue samples were collected from patients with chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP), CRS without nasal polyps (CRSsNP), and controls. The expression of HIF1α and TET2 was detected using Western blotting and immunohistochemistry. EMT markers (E-cadherin and vimentin) were also evaluated by immunohistochemistry. Primary human nasal epithelial cells (hNECs) were stimulated with CoCl 2 to mimic hypoxia. Vitamin C (VC), a TET2 non-specific activator, and small interfering RNA (siRNA) transfection of TET2 were used to further determine the role of TET2 in hypoxia-induced EMT. Finally, reactive oxygen species (ROS) and Nrf2 were measured to explore the downstream consequences of TET2 in hypoxic hNECs. Results. TET2 levels were lower in the nasal epithelium of CRSwNP patients and were positively correlated with E-cadherin but negatively correlated with vimentin in CRS. However, HIF1α exhibited the opposite pattern and was negatively correlated with TET2 expression. CoCl 2 -simulated hypoxia led to EMT and increased HIF1α in hNECs in vitro , with simultaneous downregulation of TET2 expression. Addition of VC activated TET2 expression in hNECs, but inhibited EMT and HIF1α expression. Furthermore, siRNA knockdown of TET2 contributed to the EMT in CoCl 2 -simulated hNECs despite the addition of VC. Finally, TET2 regulated the EMT in hypoxic hNECs through Nrf2 expression and ROS generation. Conclusion. TET2 was negatively correlated with HIF1α and EMT in vivo . TET2 was downregulated by HIF1α, resulting in the EMT in CoCl 2 -hypoxic hNECs via regulation of oxidative stress in vitro . Hence, TET2 might provide a new therapeutic approach for CRSwNP
研究领域:
免疫学
·
耳鼻喉科学
·
分子生物学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Yongze Liu,Lei He,Dingding Liu,Xuefeng Jiang,Feng Chen,Xiaofeng Ma,Saijie Song,Jun Zhang,Jian Shen,Xia Gao
Acute rhinosinusitis is among the most prevalent disease across the world. Recently, researchers have found that traditional Chinese medicine (TCM) can effectively treat acute rhinosinusitis. Nanomaterial-based drug delivery systems (DDSs) have attracted widespread attention in disease treatment. In this work, we herein reported curcumin-loading black phosphorus nanosheet (BP-C@PEG) nanodrugs for acute rhinosinusitis therapy. The as-prepared BP-C@PEG showed excellent solubility and stability in aqueous solution and performed sustained release behavior. In addition, it also exhibited good anti-inflammatory action for the treatment of acute rhinosinusitis. The anti-inflammation mechanism of BP-C@PEG was also studied. We found that LPS-induced inflammation in nasal epithelial cells was eased through downregulating HDAC3 and NLRP3 inflammasomes and lowering IL-1β levels. Moreover, the in vivo experiments verified that BP-C@PEG could effectively alleviate the cilium injury and the recruitment of inflammatory cells in the nasal mucosa of mice, further demonstrating its promising therapeutic efficiency in acute rhinosinusitis. Overall, this study provides a potential approach for acute rhinosinusitis therapy by using BP-C@PEG nanodrugs, which may broaden the application of TCM in the biomedical field.
研究领域:
药学
·
纳米技术
·
中医药
·
炎症研究
✓ 已验证
产品关联
DOI
高影响因子
Science Robotics
·
2025
·Vol. 10
·期 103
·
IF 27.5
Haidong Yu,Xurui Liu,Yabin Zhang,Jie Shen,Xijun Liu,Shubo Liu,Xiangyu Wang,Bonan Sun,Huihui Du,Lin Xu,Bingsuo Zou,Jianning Ding,Qingsong Xu,Li Zhang,Ben Wang
Microrobotic techniques are promising for treating biofilm infections located deep within the human body. However, the presence of highly viscous pus presents a formidable biological barrier, severely restricting targeted and minimally invasive treatments. In addition, conventional antibacterial agents exhibit limited payload integration with microrobotic systems, further compromising therapeutic efficiency. In this study, we propose a photocatalytic microrobot through a magnetically guided, optical fiber–assisted therapeutic platform specifically designed to treat bacterial infections in deep mucosal cavities. The microrobots comprising copper (Cu) single atom–doped bismuth oxoiodide (BiOI), termed CBMRs, can be guided and tracked by real-time x-ray imaging. Under external magnetic actuation, the illuminated region from the magnetically guided optical fiber synchronously follows the CBMR swarm, enabling effective antibacterial action at targeted infection sites. Upon continuous visible-light irradiation, the resultant photothermal effect substantially reduces the viscosity of pus on inflamed mucosal tissues, enhancing the penetration capability of the CBMR swarm by more than threefold compared with baseline conditions. Concurrently, atomic-level design of CBMRs facilitates robust generation of reactive oxygen species, enabling efficient biofilm disruption and reductions in bacterial viability. We validated the effectiveness of this integrated optical fiber–assisted microrobotic platform in a rabbit sinusitis model in vivo, demonstrating its potential for clinically relevant infection therapy.
研究领域:
生物医学工程
·
纳米技术
·
微生物学
·
机器人学
·
材料科学
·
医学成像
·
光动力疗法
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Kaidi Ding,Wenlong Cong,Ying Liu,Changtong Song,Haoyang Mi,Chuntai Liu,Yuhong Ma,Changyu Shen
Endoscopic surgery is an effective and common clinical practice for chronic sinusitis. Nasal packing materials are applied in nasal surgery to prevent hemorrhage and promote wound healing. In this study, a degradable polyurethane foam dressing is successfully developed as a promising nasal packing material with good biocompatibility and antibacterial capability. Specifically, quaternized chitosan (QCS) serves as the crosslinker instead of polyols to offer polyurethane foam (PUF-QCS) antibacterial capability. The PUF-QCS2.0 % (with 2.0 wt% QCS) exhibits satisfactory liquid absorption capacity (19.4 g/g), high compressive strengths at both wet (14.5 kPa) and dry states (7.7 kPa), and a good degradation rate (8.3 %) within 7 days. Meanwhile, PUF-QCS2.0 % retains long-term antibacterial activity for 7 days and kills 97.3 % of S. aureus and 91.8 % of E. coli within 6 hours in antibacterial testing. Furthermore, PUF-QCS2.0 % demonstrates a positive hemostatic response in the rabbit nasal septum mucosa trauma model by reducing hemostatic time over 50.0 % and decreasing blood loss up to 76.1 % compared to the commercial PVA nasal packing sponge. Importantly, PUF-QCS also exhibits a significant antibacterial activity in nasal cavity. This nasal packing material has advantages in post-surgery bleeding control and infection prevention. Statement of Significance The performance of a nasal packing sponge requires good mechanical properties, fast and high liquid absorption rate, effective degradability and strong antibacterial activity. These features are helpful for improving the postoperative recovery and patient healing. However, integrating these into a single polyurethane foam is a challenge. In this study, quaternized chitosan (QCS) is synthesized and used as a chain extender and antibacterial agent in preparing a degradable polyurethane foam (PUF-QCS) dressing. PUF-QCS undergoes partial degradation and exhibits effective broad-spectrum antibacterial activity in 7 days. The reduction of postoperative bleeding and infection observed in the animal experiment further demonstrates that the PUF-QCS developed here outperforms the existing commercial nasal packing materials.
研究领域:
生物材料
·
生物医学工程
·
药剂学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）