POLYMER BULLETIN
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2019
·Vol. 77
·期 2
·989-1001
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IF 1.858
Qiu Bining,Tian Hua,Yin Xueqiong,Zhou You,Zhu Li
In this study, 2-phenyl ethanol (2-PEA) was microencapsulated with natural and semisynthetic carbohydrate polymers as the wall material. The wall material components methylcellulose (MC), alginate sodium (ALG), and carboxymethyl chitosan were optimized according to the results of microscopy images, zeta potential, and 2-PEA content. The combination of MC/ALG/MC was selected as the best wall components. Cobalt chloride (CoCl 2 ) was used as the core material to prepare microcapsules whose color was measured with a colorimeter to intuitively express the water absorption of the microcapsules. The absorbed water mass and b* were related to one variable quadratic equation. The rapid release properties and the natural long-term release properties of 2-PEA microcapsules were measured.
研究领域:
食品科学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2021
·
IF 2.694
Qing Yi,Wei-Peng Chen,Xiang-yang Zhang,Yan-Yang Wu,Bin Wu,Kui Chen,Li-Jun Ji
The isobaric vapor–liquid equilibrium (VLE) data of 2-phenylethanol (2-PE) + 2-ethylphenol (2-EP), 1-phenyl-2-propanol (1,2-PP) + 2-ethylphenol (2-EP), 2-phenylethanol (2-PE) + 1-phenyl-2-propanol (1,2-PP) and 2-phenyl-1-propanol (2,1-PP) + 2-ethylphenol (2-EP) were measured by a modified Rose type still under 101.3 kPa. Both the Fredenslund test and Redlich–Kister area test were employed to verify the consistency of the experimental data. Besides, the experimental isobaric VLE data were correlated by Wilson, nonrandom two-liquid, and universal quasi-chemical models, and the corresponding parameters were obtained accordingly. All three models showed good agreement with the experimental data, and among which the Wilson model performed slightly better than the others.
研究领域:
化学工程
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物理化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
·
IF 3.412
Jinbin Liu,Jing Jiang,Yajun Bai,Tai-ping Fan,Ye Zhao,Xiaohui Zheng,Yujie Cai
Bacteria rarely produce natural 2-phenylethanol. We verified a new pathway from Proteus mirabilis JN458 to produce 2-phenylethanol using Escherichia coli to coexpress l-amino acid deaminase, α-keto acid decarboxylase, and alcohol dehydrogenase from P. mirabilis. Based on this pathway, a glucose dehydrogenase coenzyme regeneration system was constructed. The optimal conditions of biotransformation by the recombinant strain E-pAEAKaG were at 40 °C and pH 7.0. Finally, the recombinant strain E-pAEAKaG produced 3.21 ± 0.10 g/L 2-phenylethanol in M9 medium containing 10 g/L l-phenylalanine after a 16 h transformation. Furthermore, when the concentration of l-phenylalanine was 4 g/L (24 mM), the production of 2-phenylethanol reached 2.88 ± 0.18 g/L and displayed a higher conversion rate of 97.38 mol %.
研究领域:
微生物学
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生物化学
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代谢工程
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产品关联
DOI
PubMed
高影响因子
Microorganisms
·
2023
·Vol. 11
·期 12
·2954
·
IF 4.5
Shufang Sun,Nawen Tang,Kun Han,Qunqing Wang,Qian Xu
Applying plant-derived fungicides is a safe and sustainable way to control wheat scab. In this study, volatile organic compounds (VOCs) of wheat cultivars with and without the resistance geneFhb1were analyzed by GC-MS, and 2-phenylethanol was screened out. The biocontrol function of 2-phenylethanol onFusarium graminearumwas evaluated in vitro and in vivo. Metabolomics analysis indicated that 2-phenylethanol altered the amino acid pathways ofF. graminearum, affecting its normal life activities. Under SEM and TEM observation, the mycelial morphology changed, and the integrity of the cell membrane was destroyed. Furthermore, 2-phenylethanol could inhibit the production of mycotoxins (DON, 3-ADON, 15-ADON) byF. graminearumand reduce grain contamination. This research provides new ideas for green prevention and control of wheat FHB in the field.Keywords:2-phenylethanol;Fusarium graminearum;volatile organic compounds (VOCs);antifungal mechanism;metabolomics
研究领域:
植物病理学
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农学
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真菌学
✓ 已验证
DOI
PubMed
高影响因子
Microbiology Spectrum
·
2022
·
IF 9.043
Wenzhi Bao,Xing Li,Jinfeng Liu,Rong Zheng,Lijuan Liu,Haibo Zhang
Phenylpyruvate decarboxylase (PPDC) is a crucial enzyme that plays important roles in 2-phenylethanol (2-PE) biosynthesis. In our previous study, we screened a highly efficient PPDC KDC4427 from the novel 2-PE-producing strain Enterobacter sp. CGMCC 5087. Meanwhile, its decarboxylation activity of indolylpyruvate (IPyA) was also higher than other indolylpyruvate decarboxylases (IPDCs) reported so far. In this study, KDC4427 protein was purified and characterized, and its catalytic mechanisms were analyzed by biological methods. The optimum pH and temperature of KDC4427 was pH 6.5 and 35°C, respectively. The enzyme activity was relatively stable between pH 6 and 8 and over the range of temperatures from 25°C to 45°C. KDC4427 showed the highest catalytic efficiency on phenylpyruvic acid (PPA); meanwhile, it also showed high activity for IPyA and 2-ketobutanoic acid, and it was found that KDC4427 belongs to IPDCs by phylogenetic tree analysis. The coverage of the three-dimensional structure of KDC4427 and EcIPDC from Enterobacter cloacae was 96%. Leucine 542, one of the residues in the substrate-binding pocket, is replaced by isoleucine in KDC4427 compared with EcIPDC. Site-directed mutagenesis showed that the transition from leucine to isoleucine was unlikely to make KDC4427 have high catalytic activity for PPA and IPyA; the mutants at glutamate 468 almost completely lost catalytic activities for both PPA and IPyA, indicating that this glutamate was essential for the catalytic activity. Additionally, alanine 387 plays an important role in the substrate selectivity of KDC4427.IMPORTANCE Compared with the chemical synthesis of 2-phenylethanol (2-PE) by condensation of ethylene oxide and benzene, the biological synthesis of 2-PE is a potential method to replace the traditional process. This makes biotransformation gradually become the main way to produce high-quality 2-PE. Phenylpyruvate decarboxylase (PPDC) is the critical enzyme in 2-PE biosynthesis, and it is a momentous point of penetration to increase the production of 2-PE. In this regard, KDC4427 can catalyze phenylpyruvic acid (PPA) to phenylacetaldehyde more efficiently than any other PPDC previously reported. Moreover, it has high activity of indolepyruvate decarboxylases (IPDCs), which will be a great breakthrough in the synthesis of indole-3-acetic acid (IAA). With this study, we offer insights into the KDC4427 catalytic mechanism and significantly expand the toolbox of available α-ketoacid decarboxylases for application in biosynthesis.
研究领域:
生物化学
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生物技术
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酶学
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微生物学
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产品关联
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2023
·
IF 5.2
Yanyang Wu,Jiabo Rao,Qing Yi,Zhihui Ma,Bin Wu,Kui Chen,Lijun Ji
Metal-organic frameworks (MOFs) with sensitive nanospace and selective binding sites are widely used to discriminate the subtle structural. Adsorption performances of 2-Phenylethanol, 1-Phenyl-2-propanol, and 2-Ethylphenol on MOFs were studied in this paper. Eight MOFs were prepared hydrothermally and NH 2 -UiO-66(1) was chosen as the subsequent adsorbent based on the larger selectivity for both 1-Phenyl-2-propanol/2-Phenylethanol and 2-Ethylphenol/2-Phenylethanol. Studies on adsorption kinetics showed that pseudo-first-order model was suitable for adsorption performances of 1-Phenyl-2-propanol on NH 2 -UiO-66(1), while pseudo-second-order model was suitable for both 2-Phenylethanol and 2-Ethylphenol. The adsorption isotherm revealed that Langmuir model was suitable for 1-Phenyl-2-propanol, 2-Ethylphenol, and 2-Phenylethanol. Thermodynamic parameters including Gibbs free energy, enthalpy, and entropy indicated that adsorption of 1-Phenyl-2-propanol on NH 2 -UiO-66(1) was a spontaneous and exothermic physisorption process, while those of 2-Ethylphenol and 2-Phenylethanol were spontaneous and endothermic physisorption processes. The adsorption process indicated that the separation of 2-Phenylethanol from 1-Phenyl-2-propanol and 2-Ethylphenol on NH 2 -UiO-66(1) was due to the Lewis acid-base interactions and sieving effect. NH 2 -UiO-66(1) was also treated the simulated enriched 2-Phenylethanol solutions and 99.5% 2-Phenylethanol was obtained. In conclusion, NH 2 -UiO-66(1) can be a feasible adsorbent to separate 2-Phenylethanol from 1-Phenyl-2-propanol and 2-Ethylphenol for industrial application.
研究领域:
化学工程
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材料科学
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物理化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Synthetic Biology
·
2024
·
IF 3.7
Yichen Yuan,Bofan Yu,Xinzhi Zhou,He Qiao,Jiazhang Lian,Xuye Lang,Yuan Yao
The demand for controllable fragrance materials is substantial owing to their potential to impart enduring scents in a variety of applications. However, the practical application of such materials has been limited by challenges in tunable morphogenesis, structural variability, and adaptability to diverse conditions. In our study, we introduce a hybrid living material that integrates a genetically engineered strain of Kluyveromyces marxianus CBS6556 with an adaptive hydrogel. The engineered K. marxianus achieved temperature stability in 2-phenylethanol (2-PE) and 2-phenylethyl acetate (2-PEAc) production by expressing relevant genes in the 2-PE metabolic pathway using the high-temperature preferential promoter SSE1. The enhanced water retention capacity supports the metabolic activities of the encapsulated yeast cells, ensuring their survival and functionality over an extended period. Fragrance-releasing living material (FLM) is designed to controllably emit fragrance 2-PE by adjusting the microbial concentration within the hydrogel matrix. The FLM exhibits versatile adhesion capabilities, effectively binding to a spectrum of surfaces such as wood, textiles, and glass as well as to natural substrates like leaves. This adaptability enhances the material’s applicability across various settings. Furthermore, FLM can be crafted into various forms, including microbeads, fibers, and films. This research opens up new horizons for controlled fragrance release of living materials.
研究领域:
生物技术
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材料科学
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化学工程
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生物工程
✓ 已验证
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
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2021
·
IF 5.89
Yang Chen,Liu Yushi,Liu Wan-Qiu,Wu Changzhu,Li Jian
Cell-free systems have been used to synthesize chemicals by reconstitution of in vitro expressed enzymes. However, coexpression of multiple enzymes to reconstitute long enzymatic pathways is often problematic due to resource limitation/competition (e.g., energy) in the one-pot cell-free reactions. To address this limitation, here we aim to design a modular, cell-free platform to construct long biosynthetic pathways for tunable synthesis of value-added aromatic compounds, using (S)-1-phenyl-1,2-ethanediol ((S)-PED) and 2-phenylethanol (2-PE) as models. Initially, all enzymes involved in the biosynthetic pathways were individually expressed by an E. coli-based cell-free protein synthesis (CFPS) system and their catalytic activities were confirmed. Then, three sets of enzymes were coexpressed in three cell-free modules and each with the ability to complete a partial pathway. Finally, the full biosynthetic pathways were reconstituted by mixing two related modules to synthesize (S)-PED and 2-PE, respectively. After optimization, the final conversion rates for (S)-PED and 2-PE reached 100 and 82.5%, respectively, based on the starting substrate of l-phenylalanine. We anticipate that the modular cell-free approach will make a possible efficient and high-yielding biosynthesis of value-added chemicals.
研究领域:
合成生物学
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代谢工程
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生物技术
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Gankai Huang,Yubao Wang,Yan Du,Qunhua Long,Kungang Chai
Adsorption-based separation of 2-phenylethanol (2-PE) and 1-phenylethanol (1-PE) is crucial yet challenging in the petrochemical industry. In this study, we report that an ultra-micropore aluminum-based metal–organic framework (Al-MOF) (i.e., CAU-23) exhibits higher 2-PE/1-PE selectivity (6.12) and 2-PE adsorption capacity (2.52 mmol g −1 ) in liquid phase compared to its pseudo-isostructural analogue of Al-MOFs, MIL-160 and MOF-303 at 293 K. The micropore diffusion model was employed to give the best fit to the adsorption kinetics of the CAU-23 with a rate achieved higher than MIL-160 and MOF-303. Computational analysis confirmed that 2-PE preferential adsorption stems from CAU-23′s well-matched pore geometry which enables efficient molecular confinement. In combination of inspecting the static adsorption properties of 2-PE and 1-PE onto CAU-23, we found that the superior affinity of CAU-23 toward 2-PE over 1-PE originates from entropy and enthalpy effect. The remarkable 2-PE/1-PE separation capability of CAU-23 was confirmed through breakthrough curve analysis (separation factor ≈ 4). As the first Al-MOF developed for 2-PE/1-PE separation, CAU-23 offers valuable insights into the design of advanced 2-PE-selective adsorbents.
研究领域:
化学工程
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材料科学
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吸附技术
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分离科学
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计算化学
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Xiaoying Li,Chunsheng Liu,Junkai Wu,Kai Su,Xiao Xiao,Libin Zhang,B. Loye Eberhart,Caixia Chen,Fuhang Song
To deepen the understanding of peach aroma, we investigated the changes of volatile organic compound (VOC) profiles during fruit development in three fresh peach cultivars: ‘Jiucui (JC)’, ‘Zhongyoupan No. 9 (ZYP9)’ and ‘Zhongyou No. 8 (ZY8)’. The classes and contents of VOCs changed significantly during fruit ripening. The representative VOC changes were categorized into three developmental stages, with the lowest volatile content observed during the middle stage. At maturity, ester-related volatiles increased significantly, dominating the VOC profiles and accounting for 78 %, 82 % and 88 % in JC, ZYP9 and ZY8, respectively, while alcohols decreased dramatically. Multivariate analysis identified 23, 21 and 20 potential volatile markers in JC, ZYP9 and ZY8, respectively, with 9 shared markers distinguishing the different developmental stages. Odor activity values (OAVs) and olfactometry analysis highlighted 36, 29, and 26 aroma-active compounds (OAV > 1) across the cultivars, with key contributors to the mature peach aroma including hexyl acetate (OAV 860–2896), ( E )-2-hexenyl acetate, ( Z )-3-hexenyl acetate, ( E )-3-hexenyl acetate, linalool, and γ -decalactone. The fruity aroma intensified as ripening progressed, with ZY8, a cultivar with a longer maturation cycle, exhibiting the highest odor intensity. This study offers insights into the dynamic changes in VOC profiles during peach development, highlighting key aroma-active compounds and their stage-specific variations.
研究领域:
植物学
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食品科学
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园艺学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Qi Lin,Cheng Peng,Kunpeng Yu,Yanling Lin,Yongquan Xu,Lijun Li,Hui Ni,Feng Chen
The β-glucosidases known to improve tea aroma are all mesothermal enzymes, limiting their use under brewing conditions. Based on the properties analysis and molecular docking, the thermostable β-glucosidase (TPG) from Thermotoga petrophlia showed potential to enhance tea aroma. Treatment by recombinant TPG at 90 °C, the floral, sweet and grassy notes of instant Oolong tea were increased, while the roasted, caramel and woody notes were decreased. The improved floral, sweet and grassy notes were related to increase releasing of benzyl alcohol (floral), geraniol (floral), (Z)-3-hexen-1-ol (grassy), benzaldehyde (sweet) and 1-hexanol (grassy) by TPG hydrolyzing of (Z)-3-hexenyl-β-D-glucopyranoside, hexanyl-β-D-glucopyranoside (HGP), benzyl-β-D-glucopyranoside, prunasin and geranyl-β-D-glucopyranoside (GGP), respectively. Although the catalytic efficiency of TGP to GGP was about twice that to HGP, HPG was more competitive than GGP when they mixed. Combined with microstructure analysis, the structure-function relationship of TPG-influencing tea aroma were understood. This study provided the method of how to mining new function of characterized β-glucosidases, as well as a theoretical basis for the development of new tea products.
研究领域:
食品科学
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酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Ruirui Li,Dongyue Yang,Zhiyu Li,Xiaohong Tang,Ke Zhong,Yan Ding,Xiaomei Han,Xueqiang Guan,Yuxia Sun
Grapes grown in the Yantai wine region typically exhibit lower phenolic content and sugar levels, resulting in wines with relatively lower sensory quality. This study aims to assess the impact of stem-contact fermentation on the quality of wines from this region. Cabernet Sauvignon and Merlot grapes were selected winemaking, using three approaches: 100% destemming, and stem-contact fermentation with 10% and 20% grape stems, respectively. After alcoholic fermentation, the wines were analyzed for oenological parameters, phenolic compounds, color parameters, volatile compounds, and sensory evaluation. The results showed that stems did not negatively impact the oenological parameters. In Cabernet Sauvignon wines, stems resulted in a significant increase in total phenolics, anthocyanins, and tannins. In Merlot wines, stems caused a significant increase in total phenolics and a notable reduction in total anthocyanins. Stems primarily affected the anthocyanin content in both Cabernet Sauvignon and Merlot wines, with minimal effects on the proportions of individual anthocyanins. PLS-DA analysis further revealed that stems significantly affected the levels of total tannins, (−)-epigallocatechin, and total phenolic compounds in Cabernet Sauvignon wines, as well as the levels of (−)-epigallocatechin and total phenolics in Merlot wines. The presence of grape stems also significantly influenced the volatile compounds in the wine. When 20% of grapes retained their stems, there was a trend toward higher volatile content, resulting a more complex aroma profile. Overall, retaining some stems during fermentation is an effective strategy to enhance the quality of wines from the Yantai wine region.
研究领域:
食品科学
·
葡萄栽培与酿酒学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）