APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2023
·Vol. 107
·期 5
·1725-1736
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IF 5
Li Jing,Li Ziyi,Gong Hui,Ma Mengyi,Li Shuolei,Yang Huilin,Zhang Hailin,Liu Jianguo
Abstract In this study, a novel high-activity amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was identified and characterized. The recombinant enzyme was determined as a monomer with a molecular mass of 75 kDa. SaAS protein exhibited the maximum total and polymerization activities at pH 9.0 and maximum hydrolysis activity at pH 8.0. The optimum temperature for total, polymerization, and hydrolysis activities were 40, 40, and 45 °C, respectively. Under the optimal pH and temperature, SaAS had a specific activity of 108.2 U/mg. SaAS also showed excellent salt tolerance and could retain 77.4% of its original total activity at 4.0 M NaCl. The addition of Mg 2+ , Ba 2+ , and Ca 2+ enhanced the total activity of SaAS. When the conversion of 0.1 M and 1.0 M sucrose was catalyzed at pH 9.0 and 40 °C for 24 h, the ratios of hydrolysis, polymerization, and isomerization reactions were 11.9:77.4:10.7 and 15.3:53.5:31.2, respectively. The α-arbutin yield of 60.3% was achieved from 20 mM sucrose and 5 mM hydroquinone catalyzed by SaAS. Key points
• A novel amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was characterized.
• SaAS has the highest specific enzyme activity among all known amylosucrase.
• SaAS has hydrolysis, polymerization, isomerization, and glucosyltransferase activities.
Graphical Abstract
研究领域:
生物化学
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酶学
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微生物学
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2023
·
IF 11.4
Jianhui Zhuang,Zhiqiang Jiang,Dawei Chen,Jingguang Li,M. James C. Crabbe,Meiyue Qiu,Yuxin Zheng,Weidong Qu
Fipronil is a broad-spectrum insecticide used for plants and poultry. Owing to its widespread use, fipronil and its metabolites (fipronil sulfone, fipronil desulfinyl, and fipronil sulfide), termed FPM, can be frequently detected in drinking water and food. Fipronil can affect the thyroid function of animals, but the effects of FPM on the human thyroid remain unclear. We employed human thyroid follicular epithelial Nthy-ori 3-1 cells to examine combined cytotoxic responses, thyroid-related functional proteins including the sodium-iodide symporter (NIS), thyroid peroxidase (TPO), deiodinases I–III (DIO I–III), and the nuclear factor erythroid-derived factor 2–related factor 2 (NRF2) pathway induced by FPM of 1–1000-fold concentrations detected in school drinking water collected from a heavily contaminated area of the Huai River Basin. Thyroid-disrupting effects of FPM were evaluated by examining biomarkers of oxidative stress and thyroid function and tetraiodothyronine (T4) levels secreted by Nthy-ori 3-1 cells after FPM treatment. FPM activated the expression of NRF2, HO-1 (heme oxygenase 1), TPO, DIO I, and DIO II but inhibited NIS expression and increased the T4 level of thyrocytes, indicating that FPM can disrupt the function of human thyrocytes through oxidative pathways. Given the adverse impact of low FPM concentrations on human thyrocytes, supportive evidence from rodent studies, and the critical importance of thyroid hormones on development, the effects of FPM on the neurodevelopment and growth of children warrant priority attention.
研究领域:
毒理学
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环境科学
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细胞生物学
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内分泌学
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PubMed
高影响因子
INORGANIC CHEMISTRY
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2022
·
IF 5.436
Dan Zhao,Wen Liu,Ya-Nan Li,Rui-Juan Zhang,Li-Na Zhang
A modulated structure with symmetrical characteristic higher than three-dimensional is a fascinating crystallographic type, and it is randomly encountered by researchers. Herein, we prepared 0.1 mm level single crystals of Na3La(VO4)2 and determined its structure to be a (3 + 1)-dimensional modulated structure using the X-ray diffraction analysis method for single crystals. Its super space group was determined to be Pca21(0β0)000. On the other hand, we introduced Tb3+ into the Na3La(VO4)2 host lattice to fabricate phosphors Na3La1-x(VO4)2:xTb3+ and studied their photoluminescence properties. Interestingly, for the strong absorption of the Na3La(VO4)2 host lattice in the range of 200–400 nm, the traditional 330–385 nm light is unable to efficiently excite Tb3+ ions in the Na3La(VO4)2 host to generate luminescence of Tb3+. Instead, Na3La1–x(VO4)2:xTb3+ is suitable to be excited by 487 nm to generate emitting light at 543, 584, and 622 nm, due to Tb3+ characteristic 4f → 4f transitions of 5D4 → 7FJ (J = 5, 4, 3). Hence, the Tb3+-doped Na3La(VO4)2 phosphors have potential applications for white-light-emitting diodes.
研究领域:
材料科学
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晶体学
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光学
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PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2022
·Vol. 19
·期 6
·e202200286
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IF 2.745
Shu-Hua Zhang,Xian-Lin Yue,Xue Zhao,Jing Tang,Yu Yang,Rui Xu,Hao Ma,Shuai-Ming Zhu,Fu-Yao Luo,Qin Zhang,Guo-Gang Zhang,Chang-Wei Li
Longibrachiamide A ( 1 ), a new 20-residue peptaibol, along with three known ones ( 2 – 4 ), were isolated from the fungus Trichoderma longibrachiatum Rifai DMG-3-1-1, isolated from a mushroom Clitocybe nebularis (Batsch) P. Kumm, which was collected from coniferous forest of northeast China in our previous work. The structure of longibrachiamide A ( 1 ) was determined by its NMR and ESI-MS/MS data, the absolute configuration of 1 was further determined by Marfey's analyses. And the complete NMR data of 2 – 4 were also reported for the first time. The similar CD spectra of 1 – 4 showed that they all had mixed 3 10 -/α-helical conformations. Compounds 1 – 4 showed strong cytotoxicities against BV2, A549 and MCF-7 cells, and also showed moderate inhibitory effects against the tested Gram-positive bacteria, including MRSA T144 and VRE-10. Graphical
研究领域:
微生物学
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药物化学
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生物化学
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高影响因子
JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2016
·Vol. 46
·期 11
·1147-1155
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IF 2.223
Jin Biyao,Zhang Yanyu,Zhao Lianhua
Abstract PdAu 3:1/C-1 and PdAu 3:1/C-2 nanoparticles were prepared in methylene chloride and water, respectively. The syntheses featured the use of tetrabutylammonium hydroxide as a phase-transfer agent and stabilizer. Electrochemical characterization showed that compared with PdAu 3:1/C-2, PdAu 3:1/C-1 had superior ethanol oxidation reaction (EOR) activity in alkaline media. The results of X-ray powder diffraction indicated that PdAu 3:1/C-1 was an alloy, whereas PdAu 3:1/C-2 was a heterogeneous mixture of the two metals. Moreover, transmission electron microscope analyses revealed that the PdAu 3:1/C-1 nanoparticles dispersed homogeneously on the carbon carrier without evident aggregation. Further, the X-ray photoelectron spectroscopy results demonstrated that the hydroxyl content was higher on the surface of PdAu 3:1/C-1 than that of PdAu 3:1/C-2. The increase of adsorptive hydroxyl on the surface of PdAu 3:1/C-1 could facilitate the adsorption and oxidation of CO on the catalyst surface, thus improving EOR activity and catalyst stability. Graphical Abstract
研究领域:
化学
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材料科学
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电化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Keyu Lin,Zhongyu Shi,Zheyi Zhang,Yunfan Wei,Sibao Wan,Haiyan Gao,Zhen Qin
β-1,3-1,4-Glucanases have attracted significant interest because of their potential applications in various food industries. Glycoside hydrolase (GH) family 148 β-1,3-1,4-glucanases exhibit excellent thermal stability and a unique catalytic mechanism, making them particularly promising for certain food processing applications. This study investigated the module architecture enzymatic properties, catalytic mechanisms, and potential applications of a multi-domain β-1,3-1,4-glucanase (EngU) from GH family 148. The overall structure of EngU comprises three independently folding domains, namely the GH148 catalytic domain, CBM4, and the C-terminal domain. The insertion of CBM4 causes the catalytic domain of EngU to be non-contiguous in sequence. The optimum reaction conditions for EngU have been found to be pH 6.0 and 90 °C, demonstrating relatively high thermostability. EngU is an atypical β-1,3-1,4-glucanase, predominantly cleaves the β-1,3 glycosidic bonds in β-1,3-1,4-glucan. Malt saccharification experiments revealed that adding EngU (80 U/g malt) can decreased the viscosity of mash by 6.85 % and the filtration time by 28.83 %. Furthermore, EngU was found to effectively hydrolyze oat bran, producing β-glucooligosaccharides, with the main hydrolysis products being trisaccharide and disaccharide. These results demonstrate the unique module architecture and the application potential of GH family 148 β-1,3-1,4-glucanases, providing valuable insights and resources regarding this category of glycoside hydrolases.
研究领域:
生物化学
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食品科学
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DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2021
·Vol. 18
·期 5
·e2100128
·
IF 2.408
Shu-Hua Zhang,Jiewei Yang,Hao Ma,Yu Yang,Guo-Feng Zhou,Xue Zhao,Rui Xu,Dan Nie,Guo-Gang Zhang,Jun-Jie Shan,Cheng-Bin Cui,Chang-Wei Li
Five new peptaibols, longibramides A–E ( 1 – 5 ) with 11 amino acid residues, were isolated from a fungus Trichoderma longibrachiatum Rifai DMG-3-1-1, which was isolated from a mushroom Clitocybe nebularis (Batsch) P. Kumm collected from coniferous forest in the subboreal area of northeast China. The structures of longibramides A–E were determined by their spectroscopic data (NMR and MS-MS spectra), their absolute configurations were determined by X-ray diffractions and Marfey's analyses. The X-ray diffractions of longibramides A, B, and the similar CD spectra of A–E showed that they all had α -helix conformations. Longibramides B and E showed moderate cytotoxicities against BV2 and MCF-7 cells and also showed some inhibitory effects against methicillin-resistant Staphylococcus aureus MRSA T144. L- trans- Hyp was not commonly found in natural peptaibols, which was the 6 th or 10 th amino acid residue in longibramides C–E. The X-ray diffractions of longibramides A and B afforded the accuracy conformations of their secondary structures, which maybe help to interpret the structure-activity relationships of the family of peptaibols in the future. Graphical
研究领域:
微生物学
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药物化学
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结构生物学
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PubMed
高影响因子
IONICS
·
2015
·Vol. 21
·期 9
·2567-2574
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IF 1.754
Liu Guishen,Ma Yanhui,Hou Xiaodong,huang Yina,Chen Jianpeng,Zhan Guoqing,Li Chunya
1-[3-(N-Pyrrole)propyl]-3-[1-tert-butoxy-carbonylamino-propyl]imidazolium tetrafluoroborate ionic liquid was successfully synthesized and characterized. The ionic liquid was electrochemically polymerized onto a glassy carbon electrode surface using a multi-potential step technique. Morphology and electrochemical properties of the polymerized ionic liquid film electrode were studied with scanning electronic microscopy and electrochemical impedance spectroscopy. Using the polymerized ionic liquid film as a sensing platform, electrochemical behaviors of magnolol were investigated by voltammetry. Under optimal experimental conditions, the oxidation peak currents were linearly related to magnolol concentrations in the range from 5.0 × 10 −8 to 1.0 × 10 −5 mol L −1 . The detection limit calculated from the standard deviation of the intercept was 2.3 × 10 −7 mol L −1 . Practical application of the polymerized ionic liquid film electrode was demonstrated by determining magnolol in the traditional Chinese medicine. The result is consistent with that obtained using high performance liquid chromatography.
Polymerized ionic liquid film electrode fabricated with potential step technique and application in voltammetric sensing of magnolol
研究领域:
分析化学
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电化学
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DOI
高影响因子
JOURNAL OF HETEROCYCLIC CHEMISTRY
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2011
·Vol. 48
·期 6
·1434-1439
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IF 0.962
Qiang Shao,Hui Liu,Ting-Ting Wang,He-Ping Zeng
Several 3,3′-(1,4-phenylene)bis(1,5-diones) and their chalcone precursors have been prepared in good to excellent yield via aldol addition and Michael addition starting from 3-acetyl-2,5-dimethylfuran or 3-acetyl-2,5-dimethyl-thiophene with terephthalaldehyde in the presence of appropriate base NaOH or lithium diisopropylamide. The kind and amount of alkali played a critical role in improving the reaction rates and yields of the products. J. Heterocyclic Chem., (2011).
研究领域:
有机化学
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合成化学
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Juan Bi,Yuxin Liang,Yang Bai,Baoning Cui,Zhaopeng Lu,Bangsheng Li
Some studies have proven the effectiveness of high-entropy alloying in reducing lattice thermal conductivity; however, the synchronous degradation in carrier mobility limits the thermoelectric properties of high-entropy materials. Herein, we present a series of medium-entropy oxides (Sr 1/3 Ba 1/3 Ca 1/3 ) 1-x La x TiO 3 (x = 0.05, 0.1, 0.15) with different levels of La doping, breaking the trade-off between carrier and phonon transport. The medium-entropy design helps keep the carrier mobility at a moderate level while modulating the carrier concentration through La 3+ doping, thereby yielding a peak power factor value of 1842 μW/(m⋅K 2 ) at 773 K. Meanwhile, the incorporation of nano-micro-porous structure and medium entropy effect leads to a significant reduction in lattice thermal conductivity, reaching 2.3 W/(m⋅K) at 1073 K. Consequently, a ZT max value of 0.67 is obtained for the MEP-10La sample at 1073 K, which is one of the state-of-the-art ZT values reported for the SrTiO 3 -based materials. The present work provides a viable approach for the development of thermoelectric materials with enhanced performance.
研究领域:
材料科学
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热电学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Zhongyu Shi,Xiasen Wei,Yunfan Wei,Zheyi Zhang,Sibao Wan,Haiyan Gao,Zhen Qin
A novel glycoside hydrolase (GH) family 16 multi-domain β-1,3-1,4-glucanase (FsGlc16A) from Fibrobacter sp. UWP2 was identified, heterogeneously expressed, and its enzymatic properties, protein structure and application potential were characterized. Enzymological characterization showed that FsGlc16A performed the optimal catalytic activity at pH 4.5 and 50 °C with a specific activity of 3263 U/mg. FsGlc16A exhibited the substrate specificity towards oat β-glucan, barley β-glucan and lichenan, and in addition, it hydrolyzed oat β-glucan and lichenan into different β-glucooligosaccharides with polymerization degrees of 3–4, which further illustrated that it belonged to the endo-type β-1,3-1,4-glucanase. FsGlc16A was classified in subfamily25 of GH16. A ‘PXSSSS’ repeats domain was identified at the C-terminus of FsGlc16A, which was distinct from the typical GH family 16 β-1,3-1,4-glucanases. Removing the ‘PXSSSS’ repeats domain affected the binding of the substrate to FsGlc16A and reduced the enzyme activity. FsGlc16A displayed good potential for the applications, which hydrolyzed oat bran into β-glucooligosaccharides, and reduced filtration time (18.89 %) and viscosity (3.64 %) in the saccharification process. This study investigated the enzymatic properties and domain function of FsGlc16A, providing new ideas and insights into the study of β-1,3-1,4-glucanase.
研究领域:
生物化学
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酶学
·
微生物学
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食品科学
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DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2023
·
IF 8.3
Fan Wang,Yueying Zheng,Xinggang Wei,Dawei Lan,Jintao Zhu,Yingjie Chen,Ziquan Wo,Tao Wu
This study designed a composite material with internal synergistic effects among multiple components to achieve highly selective adsorption of Cu (II). Through controlled synthesis, the Fe 3 O 4 /MnO 2 (3 1 0)/ZIF-67 composite was successfully fabricated, leading to significant improvement in adsorption selectivity , capacity, and adsorption rate. The experimental results showed that the composite is of outstanding selectivity in the adsorption of Cu (II), with a partition coefficient K of Cu (II) that was 2.2–5.3 times higher than that of other coexisting ions. Moreover, the composite exhibited a remarkable adsorption capacity of 1261.0 mg g −1 and a fast adsorption rate of 840.7 mg g −1 h −1 at 298 K. Additionally, its magnetic property facilitated easy separation from wastewater, thereby enhancing its potential for commercial applications. The synergetic effect mechanism was analyzed through characterizations and DFT calculations. Furthermore, the recyclability of the composite was investigated, which showed that after seven cycles, the adsorption efficiency remained at 85% of its initial efficiency. It can be concluded that Fe 3 O 4 /MnO 2 (3 1 0)/ZIF-67 has potential to address challenges posed by heavy metal pollution in copperplating effluents.
研究领域:
环境科学
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材料科学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）