TALANTA
·
2026
·
IF 6.7
Peipei Xu,Junpeng Su,Junhua Zhan,Junbo Huang,Junjie Ren,Yu Chen,Lu Chen,Hanhao Lu,Jingbo Chen,Zhenhua Liu,Jing Zhuang,Yudong Lu
Therapeutic drug monitoring (TDM) of mitoxantrone (MIT) is essential for balancing efficacy and toxicity, yet current methods are limited by complex pretreatment, long turnaround times, and matrix interference. Herein, we developed a SERS sensor based on a pectin/AgNPs@CMC/CS/PAAM composite hydrogel for separation-free detection of cationic anticancer drugs in plasma. Fabricated via one-step UV photopolymerization, the hydrogel's dense network provides size-sieving to exclude plasma proteins, while abundant carboxyl groups confer a strong negative surface charge for selective electrostatic enrichment of positively charged drug molecules. This dual mechanism enhances specificity toward cationic analytes in complex matrices. Using MIT as a model, the sensor achieved 82.6%–101.7% recoveries in 10% plasma, a detection limit of 4.7 × 10 −10 M, and excellent reproducibility. Requiring only simple dilution, this platform offers a practical and ultrasensitive tool for TDM of cationic drugs in combination chemotherapy.
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2022
·
IF 3.428
Jia Wang,Deyue Yan,Wei Huang
A fluorescence metal-organic framework [Zn 3 (APT) 4 ]·2H 2 O (Zn-APT) was synthesized from Zn(NO 3 ) 2 ·6H 2 O and 2-amino-6-purinethiol (ATP) by the solvothermal reaction. Zn-APT has a novel compact structure with a nbo topology type. The analyses of single-crystal diffraction reveal that Zn-APT crystallizes in a cubic crystal system and F d 3 ¯ c space group. Based on a large amount of π-conjugated organic ligands of ATP and d 10 metal center of Zn 2+ , Zn-APT can emit strong luminescence. It can be used as a fluorescent probe to detect Fe 3+ ion effectively. The detection limit is 0.28 μM and K sv (quenching efficiency) is 2.1 × 10 4 M −1 . In addition, Zn-APT can adsorb hemoporphyrin dihydrochloride to produce a red-brown crystal Zn-APT-PH. Interestingly, it can also be used to detect Fe 2+ ion efficiently with the detection limit of 0.12 μM and K sv of 1.9 × 10 4 M −1 .
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Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 9
·3576-3584
·
IF 8.067
Yu Zhang,Xin Xu,Bing Yan
Lanthanide metal organic frameworks (Ln-MOFs) as excellent fluorescent materials have shown enormous potential applications, especially in fluorescence sensing and anticounterfeiting. However, very few examples of Ln-MOFs can realize both fluorescence sensing and anticounterfeiting simultaneously based on the same fluorescence response mechanism. Herein, a multifunctional platform constructed by a multicolour-switchable fluorescent Ln-MOFs (isostructural Eu-MOFs and Tb-MOFs) triggered by anti-cancer drugs (6-MP and 6-TG) were prepared by a simple hydrothermal method. The multifunctional platform is composed of a sensing platform for detecting anti-cancer drugs and an anticounterfeiting platform for information on encryption and decryption. The sensing platform based on Eu-MOFs exhibits great selectivity, high sensitivity, and low detection limit toward anti-cancer drugs (6-MP and 6-TG) with the assistance of sensing intermedium (Cu2+). On the other hand, multiple and switchable fluorescent colours (purple–red–blue/cyan–green–blue) triggered by Cu2+ and 6-MP/6-TG make the prepared Ln-MOFs inks good luminescent materials to build an anticounterfeiting platform with high-level security. This work illustrates the inspiring and promising applications of luminescent Ln-MOFs in anti-drug sensing and information of anticounterfeiting for the first time and will open the possibility of the design of Ln-MOFs with tuneable and multiple luminescence as a multifunctional optical platform.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Bingyong Lin,Yueliang Wang,Yuanyuan Yao,Lifen Chen,Yanbo Zeng,Lei Li,Zhenyu Lin,Longhua Guo
Surface-enhanced Raman scattering (SERS) is a super-sensitive analysis technology based on the target molecular fingerprint information. The enhancement of local electromagnetic field of the SERS substrate would increase the target molecules’ Raman intensity which adsorb on the surface of nanoparticles. However, the existing adhesive macromolecules in the complex mixed sample would interfere with the adsorption of small target molecules, and it weakens the Raman intensity of target molecules. Microgels are one of the potential materials to suppress the interference of adhesive macromolecules and to avoid the complex pretreatments. However, most of the current microgel synthesis methods involve complex operations with precise instrumentation or the interference of oil and organic reagents. In this work, a simple and oil-free method was proposed to synthesize the gold nanobipyramid (Au NBP)@Ag@hyaluronic acid microgel via the condensation reaction of carboxyl and amino groups. As a proof-of-concept demonstration for small-molecule detection, the rhodamine 6G (R6G) molecules were allowed to enter inside the microgel through the meshes and adsorb on the surface of Au NBP@Ag nanoparticles within 30 min, while the macromolecule (bovine serum albumin in this case) was retained outside the microgel in the meantime. In addition, under the combined action of lightning rod effect of Au NBP and surface plasmon resonance effect of silver render the microgels with high SERS activity. The synthetic Au NBP@Ag@hyaluronic acid microgels were applied to detect 6-thioguanine in the human serum without any pretreatment process, and it showed a high signal enhancement and stable SERS signal, which can satisfy the requirement of clinical diagnosis. These results show that the proposed microgels have potential applications in the field of point-of-care testing.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Shuai Yue,Xiao-Ting Sun,Yue Wang,Wen-Shu Zhang,Zhang-Run Xu
Surface-enhanced Raman scattering (SERS) spectroscopy is an ultrasensitive analytical technique with high specificity, making it an ideal candidate for therapeutic drug monitoring. However, SERS measurement of drugs in biological samples is inevitably affected by the proteins adsorbed nonspecifically on metal nanoparticles, so the drugs usually require separation before SERS detection. In this work, semipermeable alginate microparticles containing gold nanoparticles were fabricated via a centrifugal microfluidic chip, and served as a reliable substrate for SERS detection of 6-thioguanine in serum without protein removal. In comparison with the conventional microfluidic methods using oil for droplet generation, this oil-free method can fabricate more clean and versatile microparticles. The microparticles were characterized by size/charge selectivity and pH response, which effectively improved anti-interference ability and SERS sensitivity for 6-thioguanine detection in serum. The microparticles were further endowed with magnetic property and SERS activity simultaneously by forming Janus structures embedded with magnetic nanoparticles and gold nanoparticles separately in independent hemispheres, which was convenient for manipulation in SERS detection. More broadly, the microparticles are potentially applicable for SERS detection of other small molecules in complex real samples.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Wen-Shu Zhang,Ya-Ning Wang,Yue Wang,Zhang-Run Xu
6-thioguanine is an anticancer drug with narrow safe dose range and intense toxic reactions, so it is urgently necessary to evaluate its clinical usage. Reproducibility and analysis speed are important factors to take into account when analyzing drug concentration in body fluids. In this work, we fabricated a droplet-based microfluidic chip for detecting 6-thioguanine in human serum by using the surface-enhanced Raman scattering (SERS) technique. The easily-prepared Au nanoparticles were used as SERS substrates, and reagent mixing and signal acquiring happened in droplets one by one. This assay device is endowed with the superiority in improving determination repeatability with coefficient of variation of no more than 5% (n = 25) and high speed of 10 s each assay. The SERS peak at 1295 cm −1 originated from N1-C6 stretching vibration mode of purine ring was selected for quantitating 6-thioguanine. The signals indicated a satisfied linear relationship with the concentrations of 6-thioguanine in the range of 0.010 μM to 10 μM, with a detection limit of 0.032 μM. The droplet-based microfluidic SERS system was successfully applied for credible and rapid analysis of 6-thioguanine in human serum, and is pretty promising for performing the therapeutic drug monitoring.
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JOURNAL OF APPLIED TOXICOLOGY
·
2015
·Vol. 35
·期 11
·1415-1426
·
IF 2.982
Xu Wang,Ziqiang Tan,Yuanhu Pan,Awais Ihsan,Qianying Liu,Lingli Huang,Guyue Cheng,Dongmei Chen,Yanfei Tao,Zhenli Liu,Zonghui Yuan
Aditoprim (ADP), a new developed dihydrofolate reductase (DHFR) inhibitor, has great potential in clinical veterinary medicine because of its greater pharmacokinetic properties than structural analogs. Preclinical toxicology studies were performed to assess the safety of ADP including an acute oral toxicity test, a subchronic toxicity test and five mutagenicity tests. In the acute oral toxicity test, ADP was administered singly by oral gavage to Wistar rats and Kunming mice. The LD 50 calculated was 1400 mg kg –1 body weight (BW) day –1 in rats and 1130 mg kg –1 BW day –1 in mice. In a subchronic study, Wistar rats were administered ADP at dose levels of 0, 20, 100 and 1000 mg kg –1 diet for 90 days. Significant decreases were observed on body weight and food efficiency in the high-dose group. Treatment-related changes in clinical serum biochemistry were found in the medium- and high-dose groups. Significant increases in the relative weights of livers and kidneys in females and testis in males in the 1000 mg kg –1 diet, and significant decrease in relative weights of livers in males in the 100 mg kg –1 diet were noted. Histopathological observations revealed that the 1000 mg kg –1 ADP diet could induce lymphocytic infiltration and hepatocytic necrosis near the hepatic portal area. The genotoxicity of ADP was negative in tests, such as the bacterial reverse mutation assay, mice bone marrow erythrocyte micronucleus assay, in vitro chromosomal aberration test, in vitro cho/hgprt mammalian cell mutagenesis assay and mice testicle cells chromosome aberration. Based on the subchronic study, the no-observed-adverse-effect level for ADP was a 20 mg kg –1 diet, which is about 1.44-1.53 mg kg –1 BW day –1 in rats. Copyright © 2015 John Wiley & Sons, Ltd.
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NANOTECHNOLOGY
·
2016
·Vol. 27
·期 9
·095603
·
IF 3.573
Jingjie Cui,Jing Chen,Shaowei Chen,Li Gao,Ping Xu,Hong Li
Cancer is a cell dysfunction disease. The detection of cancer cells is extremely important for early diagnosis and clinical treatments. At present, the pretreatment for the detection of cancer cells is costly, complicated and time-consuming. As different species of the analytes may give rise to specific voltammetric signals at distinctly different potentials, simple potential sensing has the specificity to detect different cellular species. By taking advantage of the different electrochemical characteristics of normal cells, cancer cells and biointeractions between anticancer drugs and cancer cells, we develop a specific, sensitive, direct, cost-effective and rapid method for the detection of cancer cells by electrochemical potential sensing based on Au/TiO2 nanobelt heterostructure electrodes that will be of significance in early cancer diagnosis, in vitro screening of anticancer drugs and molecular biology research.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2016
·
IF 2.76
Hongli Li,Xiaoli Chong,Yufeng Chen,Lei Yang,Lan Luo,Bing Zhao,Yuan Tian
In this paper, silver nanoparticles-coated silicon wafer (AgNPs@Si) was used as surface-enhanced Raman scattering (SERS) substrate for detection of 6-Thioguanine (6-TG). The influence of experimental parameters including pH value and soaking time on SERS performance were examined. The SERS characteristic peak of 6-TG at 1303 cm −1 was selected for quantity analysis. With the 521 cm −1 band of silicon as internal standard, band intensity ratio of 6-TG to silicon, that was I 1303 / I 521 , could be used for quantification. Under the optimum conditions, I 1303 / I 521 exhibited a linear relationship with the concentration of 6-TG in the range of 0.1–15.0 μmol L −1 and detection limit (S/N = 3) was 0.065 μmol L −1 . The corresponding correlation coefficient of the linear equation was 0.998. This method based on AgNPs@Si was successfully applied to the detection of 6-TG in two kinds of commercial tablets.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Junling Duan,Yuanhong Zhang,Yanbin Yin,Houshen Li,Jun Wang,Lusheng Zhu
A facile fluorescence “on-off-on” approach for sensing 6-Thioguanine (6-TG) and Hg 2+ was designed based on g-C 3 N 4 nanosheets. It was found that in the absence of Hg 2+ , the addition of 6-TG to g-C 3 N 4 nanosheets solution resulted in the fluorescence quenching of g-C 3 N 4 nanosheets (turn off). However, in the presence of Hg 2+ , the quenched fluorescence of g-C 3 N 4 nanosheets was recovered (turn on), because the high affinity of Hg 2+ and 6-TG resulted in the loss of molecular interactions between 6-TG and g-C 3 N 4 nanosheets. The quantitative detection of 6-TG was obtained via the fluorescence quenching of g-C 3 N 4 nanosheets with a detection limit of 65 nM. Meantime, the 6-TG-g-C 3 N 4 nanosheets system could be used as a fluorescent “turn on” sensor for Hg 2+ . The detection limit for Hg 2+ was 37 nM. The proposed method showed high selectivity for the detection of 6-TG and Hg 2+ . In addition, it was successfully applied to detect Hg 2+ in real water samples.
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Virulence
·
2024
·
IF 5.5
Xin Wang,Feiyang Pu,Xuanye Yang,Xili Feng,Jiayou Zhang,Kai Duan,Xuanxuan Nian,Zhongren Ma,Xiao-Xia Ma,Xiao-Ming Yang
Influenza A virus (IAV) poses threat to patients receiving immunosuppressive medications since they are more susceptible to infection with severe symptoms, and even death. Understanding the direct effects of immunosuppressants on IAV infection is critical for optimizing immunosuppression in these patients who are infected or at risk of influenza virus infection. We profiled the effects of ten immunosuppressants, explored the antiviral mechanisms of immunosuppressants and demonstrated the combination effects of immunosuppressants with the antiviral drug oseltamivir in IAV-infected cell models. We found that mycophenolic acid (MPA) strongly inhibited viral RNA replication via depleting cellular guanosine pool. Treatment with 6-Thioguanine (6-TG) promoted viral protein degradation through proteasomal pathway. Filgotinib blocked mRNA splicing of matrix protein 2, resulting in decreased viral particle assembly. Furthermore, combined treatment with immunosuppressants and oseltamivir inhibited IAV viral particle production in an additive or synergic manner. Our results suggest that MPA, 6-TG and filgotinib could be the preferential choices for patients who must take immunosuppressants but at risk of influenza virus infection.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Ya-Ning Wang,Ying Zhang,Wen-Shu Zhang,Zhang-Run Xu
A novel surface-enhanced Raman scattering substrate was developed through in situ growing gold nanoparticles inside the mesoporous graphitic carbon nitride with a large surface area. The mesoporous structure afforded support for loading more gold nanoparticles, defined the nanoparticle diameter and ensured uniform distribution of the nanoparticles. Crystal violet used as a Raman probe was detected by SERS based on the hybrids with an enhancement factor of 5.0 × 10 5 , and the relative standard deviation of the peak intensity (at 1174 cm −1 and 1618 cm −1 ) were 6.2% and 6.1% (n = 30) for solid samples, and 5.1% and 5.0% (n = 11) for liquid samples. The high SERS activity was attributed to abundant hot spots formed by high-density gold nanoparticles and chemical enhancement from mpg-C 3 N 4 , and the good reproducibility was due to controlled size and uniform distribution of the gold nanoparticles. An antineoplastic drug, 6-thioguanine was detected with a wide linear range from 0.60 μM to 48 μM and a low detection limit of 0.24 μM. The mesoporous g-C 3 N 4 embedded with gold nanoparticles significantly improved the SERS sensitivity and reproducibility for 6-thioguanine detection, and it holds promising potential in noninvasive and ultrasensitive drug monitoring.
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