Analytical Methods
·
2019
·Vol. 11
·期 45
·5793-5802
·
IF 2.378
Rui Zhang,Mengxin Zhang,Yi Zhang,Heng Yan,Xing Li,Wei-hong Xie
A screening method for the simultaneous detection of the synthetic food dyes tartrazine (TA), Sunset Yellow (SY), Allura Red (AR), Ponceau 4R (PO), Amaranth (AM), and Bright Blue (BB) in different samples was developed. The measurements were performed on a spectrophotometry microplate reader and the concentrations of the dyes in samples were calculated using a multi-primary linear equation group. Standard solutions containing dye concentration ranges from 4–35 μg mL−1 and carbonated beverages from supermarkets were used to evaluate the precision and accuracy of the method. Six-equation groups and three-equation groups were used to predict the dye concentrations in the mixtures or in the drinks. The results indicated that the three-equation groups gave better predictions for dyes, both in the standard mixture solutions and in the carbonated beverages. The recoveries of the dyes from the drink samples were 70–108% when the spiked concentrations were 5 μg mL−1 and 10 μg mL−1, with a RSD range of 0.01–0.33%. In comparison with high performance liquid chromatography (HPLC), the method could find most of the additive dyes in the drinks with a low detection limit of around 2 μg mL−1. The standard deviations of six repeat measurements from one plate and three repeat measurements among three different plates were both within ±0.5 μg mL−1. We also tried to identify three red dyes with highly overlapping spectra: AR, PO, and AM; the results indicated that the method could also identify the three red dyes in mixed solutions.
研究领域:
分析化学
·
食品安全
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产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL CHEMISTRY
·
2025
·Vol. 80
·期 3
·448-454
·
IF 1.1
Yang Danyi,Lan Jingwei,Chen Jiao,Wu Yuyu,Qian Wen,Han Jiangbin
The aim of the present study was to develop a sensitive, high-throughput 96-well plate ultraviolet spectrometric method for the determination of peroxide value at trace levels in reagents and packaging materials. The analysis was carried out under acid-free conditions to minimize or prevent reactions between iodine molecules and oxygen in the air, thereby ensuring the accuracy and repeatability of the experiments. The wavelength was set at 360 nm. The analytical procedure was validated by evaluating linearity ( r 2 = 0.9998), precision (intra- and inter-day precision were less than 0.90 and 6.20%, respectively.), accuracy (100–110% of the spiked amount), and limits of detection ( LOD ) and quantification ( LOQ ). The LOD and LOQ were confirmed to be 20 and 50 ng/mL, respectively. This high-throughput technique reduces the risk of chemical changes during analysis due to time inconsistencies, which benefits the accuracy and reproducibility of results. The proposed method was then successfully applied to determine trace amounts of peroxides in ethyl acetate and packaging material samples. Overall, the present study offers an accurate and sensitive method for measuring trace-level peroxides.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Nanfack V.D. Carole,Lina Sheng,Jian Ji,Shang Wu,Yinzhi Zhang,Xiulan Sun
Ensuring the safety and high quality of food relies on promptly and clearly identifying foodborne diseases, including Salmonella . Nucleic acid testing plays an important role in ensuring food safety. Commercially available high-throughput molecular extraction technologies in-field application is limited as they are reliant on specialized and expensive equipment. The 96-well microplate recycling through biopolymer inner surface coating, was confirmed with FTIR, XRD, and XPS. Once optimized the DNA extraction protocol was equipment-free, user and eco-friendly while reducing sample contamination. Using the biopolymer microplate, the extraction and direct LAMP amplification of Salmonella spp. from milk samples were assessed. The gDNA had an average 260/280 ratio of 1.97, with a DNA yield of 16.88 ± 5.40 μg·mL -1 from 9 × 10 10 CFU·mL -1 Salmonella . Subsequently, using acid chrome K we visually detected the presence of Salmonella . The quantity of bacteria was correlated to the increase in the blue intensity of the color indicator. A detection limit of 1.54 CFU·mL -1 was achieved with good specificity and sensitivity in an equipment free setting. This study successfully established a 96-well plate-based high-throughput equipment free DNA extraction method. The methods enable the high-throughput extraction and amplification of 96 samples concomitantly within 1hr 30 min corresponding respectively to a total of 1536 and 768 samples within a working day treated by a researcher without the use of specialized and expensive equipment. The polymeric modified microplate was used for high-throughput DNA extraction and direct molecular amplification for pathogen detection. High-throughput DNA extraction no longer requires cumbersome equipment.
研究领域:
食品安全
·
分子生物学
·
微生物学
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生物技术
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DOI
高影响因子
ANALYST
·
2019
·Vol. 145
·期 1
·172-176
·
IF 4.019
Qian-Yu Zhou,Xin-Ying Zhong,Ling-Li Zhao,Li-Juan Wang,Ying-Lin Zhou,Xin-Xiang Zhang
Single nucleotide polymorphisms (SNPs) have been proven to be important biomarkers for disease diagnosis, prognosis and disease pathogenesis. Here, taking the advantages of a self-assembled oligonucleotide sandwich structure and robust chemical reactions, we have developed a simple, high-throughput and effective colorimetric analytical technique termed CuAAC-based ligation-assisted assays (CuAAC-LA) for SNP detection using a DNA-BIND 96-well plate. With the 5′-azide and 3′-alkyne groups labelled on two oligonucleotide probes, the target DNA can direct a Cu(I)-catalyzed alkyne–azide cycloaddition (CuAAC) click reaction. Since the small difference in duplex stability caused by a single-nucleotide mismatch was amplified by the steric effects of these reactive groups for the ligation reaction of an unstable duplex, CuAAC-LA exhibited an ultra-sensitive discrimination ability for a mutant type target in the presence of large amounts of wild type targets. As low as 0.05% SNP could be clearly detected, which was better than most previously reported methods by various DNA ligases, indicating that a simple and rapid synthetic method i.e., the DNA template-directed click reaction held the potential to replace the ligase for SNP detection.
研究领域:
分子生物学
·
基因组学
·
生物技术
·
分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Jiankun Cao,Sihui Liang,Hao Zhang,Qian Xu,Wenliang Ji
In this work, sodium 4-styrenesulfonate functionalized polyacrylonitrile nanofibers mat (SS/PAN NFM) was firstly prepared and applied as 96-well plate solid–phase extraction adsorbent for quantitative determination of seven β-agonists residues in pork samples. The functional modification endowed the SS/PAN NFM with superior adsorption performance for target β-agonists. The adsorption process is spontaneous (Δ G < 0), the initial adsorption rate can reach 6.03–9.09 mg/g/min and the maximum adsorption capacity is calculated to be 48.3 mg/g at 298 K. Moreover, SS/PAN NFM can be reused for 12 times without degradation in adsorption capability. Combined with UPLC–MS/MS, the limits of detection can reach 0.006–0.24 μg/kg, the recoveries ranged from 87.2% to 111% and the relative standard deviations of intra-day and inter-day precisions were in the scope of 1.75%–11.6% and 5.08%–13.5%, respectively. The obtained results fully demonstrated the practicability of this method in preventing the hazard of β-agonists residues.
研究领域:
分析化学
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食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 37
·20549-20556
·
IF 2.936
Sihui Hong,Yongxin She,Xiaolin Cao,Miao Wang,Chao Zhang,Lufei Zheng,Shanshan Wang,Xingbin Ma,Hua Shao,Maojun Jin,Fen Jin,Jing Wang
In this study, a direct competitive biomimetic enzyme-linked immune-sorbent assay (BELISA) based on a molecularly imprinted nanomembrane as an artificial antibody was developed for the determination of triazophos in real samples. The imprinted film was directly synthesized on the well surface of a 96-well plate using a dummy molecular template under the conditions of thermal polymerization. The developed BELISA using a hapten of triazophos as an enzyme-labeled probe is much more sensitive, simple, quick, steady and inexpensive than the other instrumental and immuno assay methods. Under optimal conditions, the linear range of the method was 0.001–10 000 μg L−1 with a good regression coefficient of 0.977. The sensitivity (IC50) and the limit of detection (LOD) of BELISA were 428 μg L−1 and 0.001 μg L−1, respectively. This method was performed to detect triazophos in cabbage and apple samples, and showed excellent recovery and relative standard deviations (RSDs) ranging from 70.5 to 119.8% and from 5.2 to 19.7%, respectively. The results correlated well with those obtained using high performance liquid chromatography-tandem mass spectrometry.
研究领域:
分析化学
·
环境科学
·
食品安全
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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