CATALYSIS LETTERS
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2024
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IF 2.3
Xiang Xiaoyan,Liao Xu,Yan Qijiang,Yuan Qiulan,Zhou Long,Zhang Ruiming,Lin Jinqing,Lin Xihuang
In this paper, porous Brønsted acidic ionic liquid modified catalyst ILs-AB-8 was successfully synthesized by using 3 steps of Friedel–Crafts hyper-crosslinking synchronous chloromethylation, imidazlole grafting and sulfonic functionalization using macroporous polystyrene AB-8 resin as a raw material. The porous ionic liquid immobilized catalyst ILs-AB-8 exhibits a hierarchical pore structure by the coexistence of abundant macropore, mesopore, and micropore. The catalyst IL 2 -AB-8 with dual Brønsted acid sites(-SO 3 H acid site and p-methylbenzenesulfonic acid site), large surface area (327.19 m 2 /g), high ionic liquid loading amount (IEC 2.03 mmol/g) displayed excellent catalytic activity, with methyl oleate yield up to 95.65% at 338 K in 100 min. The enhanced catalytic performance of IL 2 -AB-8 can be ascribed to the novel Friedel–Crafts hyper-crosslinking process in conjunction with chloromethylation, coupled with th in situ generated activating chloromethyl group incorporated onto the surface of the AB-8 carrier. The hyper-crosslinking approach also effectively transforms the macroporous AB-8 carrier into a multi-level pore structure. This work highlights that IL 2 -AB-8 with high specific surface area, ionic liquid loading content, and strong covalent connection with carrier was coordinated to provide an efficient metal-free heterogeneous catalyst for biodiesel production. Graphical Abstract
研究领域:
化学工程
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催化材料
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可再生能源
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2025
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IF 4
Ziwei Yang,Zhiwei Zhang,Ying Zhang,Xiaoqiang Chen
As the first step of resource utilization, the extraction and separation of active ingredients play an important role in the evaluation and application of their activity. In this study, a macroporous resin purification process was optimized to obtain high-purity sea buckthorn flavonoids (SBF). The AB-8 resin exhibited optimal adsorption and desorption properties, with adsorption and desorption rates of 75.25% and 84.78%, respectively. Under optimal purification conditions, the purity of the flavonoid extract was enhanced from 17.17 ± 0.19% to 63.2 ± 0.16%. Kinetic and thermodynamic analyses demonstrated that the purification of SBF on AB-8 resin conformed to the pseudo-secondary kinetic model and the Langmuir adsorption isotherm model. The major components of the SBF were taxifolin, isoquercitrin, narcissoside, and quercetin. The SBF not only inhibited lipopolysaccharide-induced nitric oxide production but also exerted significant anti-inflammatory effects, suppressing the expression of proteins such as nitric oxide synthase, tumor necrosis factor, interleukin 6, and interleukin 1β and inhibiting apoptosis. Therefore, the purified SBF demonstrated improved anti-inflammatory activity, which can be applied in the development of drugs and functional foods.
研究领域:
植物化学
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药理学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
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2023
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IF 4
Weng Di,Tian RongRong,Jin HaiXin,Zha ShengHua,Zhao QingSheng,Zhao Bing
In this study, a method to enrich forsythiaside A (FA) from the ball-milled powder of Forsythia suspensa leaves was established. Forsythiaside A was extracted by heating and stirring, and the extraction process was optimized by response surface methodology using the independent variables of extraction temperature, extraction time, liquid-to-material ratio, ethanol concentration, and pH value of the solution, respectively. Then five types of macroporous resins with different polarities were studied to explain the adsorption mechanism by static adsorption and desorption experiments. Different models were used to study the static adsorption thermodynamics and kinetic process of AB-8 resin on FA extracts, and optimal conditions of the process were optimized. Finally, the antioxidant abilities of purified FA were evaluated. The results showed that the optimum extraction conditions were 2.2 h, 53.4 °C, 45.7 mL/g, 60% ethanol, and pH 6, and the extraction efficiency of forsythiaside A could achieve 120.96 mg/g. The AB-8 macroporous resin was the most suitable resin with high adsorption and desorption rates. It was found that the Temkin model was able to calculate the adsorption parameters accurately. The adsorption kinetic process of the resin was in accordance with the pseudo-second-order kinetic model. The optimal process conditions were as follows: the optimum loading concentration of 5.83 mg/mL, the loading rate of 1 BV/h, and the loading volume of 7.51 BV; in the desorption process, deionized water was used to remove impurities first, and then, 30% ethanol was used with an elution rate of 1 BV/h. The purity of FA increased from 20.52 to 81.58%. The purified FA exhibited superior scavenging ability for DPPH radicals and ABTS radicals. Cytotoxicity experiments proved that it was safe and nontoxic and was capable of promoting cell growth. In conclusion, the present study provides a theoretical basis for industrially extracting and purifying FA on a reliable basis.
研究领域:
植物化学
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药理学
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天然产物研究
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产品关联
DOI
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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2025
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IF 1.9
Yang Li,Wei Zhao,Qifeng Li,Jiayu Zhang,Anquan Yang,Min Xie,Ranhua Xiong,Chaobo Huang
Erigeron breviscapus, a medicinal plant rich in flavonoids with diverse pharmacological properties is an important resource. To maximize the utilization of these valuable bioactive compounds, ultrasound-assisted extraction combined with response surface methodology was used to optimize the extraction conditions for total flavonoids. After comparing six macroporous resins’ adsorption and desorption characteristics, AB-8 was selected for enriching total flavonoids, and its adsorption kinetics and thermodynamics were investigated. The following parameters were chosen as optimal for the dynamic adsorption/desorption experiments using an AB-8 filled column: sample loading concentration of 1.14 mg/mL, sample loading flow rate of 2 BV/h, elution solvent of 70% ethanol, desorption flow rate of 1 BV/h, elution solvent volume of 5.5 BV, pH of 2, temperature of 25 °C. After enrichment, the concentration of flavonoids was 5.16 times higher than that of crude extract. LC-MS identified 54 flavonoid molecules with excellent antioxidant properties.
研究领域:
植物化学
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药学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Food Science and Technology
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2022
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IF 2.602
PAN Feng,LI Sanhua,ZHU Xinting,YANG Jianbo,WEN Jing,SONG Changwei,LUO Xirong,RUAN Guoyong,LIU Yun
Resin AB-8 was screened from seven resins with different polarity and particle size because of its better decoloration and deproteinization efficiency to polysaccharide from the root of Actinidia valvata (AvPs), and then the optimized parameter conditions were applied by the single-factor experiments and were shown as follows: a concentration of crude polysaccharide of 3 mg/mL, an adsorption time of 40 min, an initial pH of 7.0, a flow rate of 1.5 BV/h. In addition, the results of HPGPC, FT-IR, PMP-HPLC-DAD, 1H NMR and Congo red test showed that the sugar chain, structural group, monosaccharide composition and triple-helix conformation structure of AvPs were not broken during resin AB-8 treatment. Moreover, AvPs could keep the stability of biological activity after the resin AB-8 treatment, and revealed an obviously antioxidant and anticancer activities in vitro as well. This research suggests that this method is promising for the deproteinization and decolorization of AvPs which could be explored as a potential antioxidant or anticancer agent for use in functional food or medicine.
研究领域:
药理学
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生物化学
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食品科学
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医学
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产品关联
DOI
高影响因子
RSC Advances
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2015
·Vol. 5
·期 114
·93937-93944
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IF 3.84
Ji Li,Zhigang Han,Yongpeng Zou,Bo Yu
In this study, an efficient microwave-assisted extraction (MAE) method using green deep eutectic solvents (DESs) was developed for the extraction of the major catechins in Camellia sinensis leaves. A series of choline chloride-based DESs were prepared, and DES-4, which was composed of choline chloride and lactic acid, was observed to possess the best extraction performance for the target catechins. Moreover, the main factors affecting the extraction efficiency were optimized using Box–Behnken design (BBD) combined with response surface methodology (RSM), and the optimal conditions were as follows: temperature 66 °C, duration 8 min and solvent/material ratio of 35 mL g−1. Under these conditions, a total extraction yield of 153.7 ± 5.2 mg g−1 was obtained. In addition, the separation of the target catechins from DES-based extraction solution was performed using AB-8 macroporous resin with recovery yields from 75.2% to 86.1%. The present study provided fundamental data for the development of DES-based extraction methods in food and pharmaceutical fields.
研究领域:
食品科学
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药学
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化学工程
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2017
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IF 3.181
Shangying Yang,Ying Li,Dongying Jia,Kai Yao,Weijuan Liu
The synergistic effect of hot water extraction (HWE) and enzyme-assisted extraction (EAE) optimized by the Box-Behnken design (BBD) was investigated for a more efficient extraction method for mulberry leaf polysaccharide (MLP) with an improved dried crude MLP yield of 24.04 ± 0.98% under the optimal conditions for HWE (liquid-solid ratio 30, extraction temperature 85 °C and time 60 min) and EAE (pH 6.5, extraction temperature 45 °C and time 50 min), respectively. Furthermore, the static absorption of AB-8 macroporous resin performed the best among decoloring agents for the further crude MLP purification, from which three ethanolic fractions (MLP-40, MLP-60 and MLP-80) were derived with their characterization in carbohydrate, protein and total phenolic contents. The molar ratio of the monosaccharide composition in MLP-80 was 1:14.8:29.3:0.8:0.5:2.2 corresponding to mannose, rhamnose, ribose, glucose, galactose and galacturonic acid, respectively. In addition, the prebiotics effect of MLPs was investigated compared with conventional inulin and oligofructose, in which MLP-80 was finally demonstrated itself as the most promising natural additives in liquid dairy products like yoghourt.
研究领域:
食品科学
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植物化学
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生物技术
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2020
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IF 3.594
Na Guo,Yong-Peng Zou,Hong-Kun Li,Ping Kou,Zhi-Ming Liu,Yu-Jie Fu
Conventional approaches for extraction and enrichment of phytochemicals from plant matrix have many drawbacks such as high organic solvent consumption, low extraction yields, time-consuming and large labor for the enrichment process. In the present study, ultrasound-assisted deep eutectic solvents extraction (UAE-DESs) was developed for extraction and determination of the linarin from Chrysanthemum indicum L. flower (CIF). And a feasible strategy was performed to separate and recover the linarin from denatured DESs using AB-8 macroporous resins as adsorbents. Under the optimal extraction conditions, the maximum linarin extraction yield reached 14.23 mg/g with liquid–solid ratio 32 mL/g, extraction power 340 w, extraction time 32 min and deep eutectic solvent of choline chloride-ethylene glycol with the molar ratio 1:2 and water content 30% (v/v) as a extraction system, which was 1.21 times higher than 80% ethanol extraction. After macroporous resins enrichment, the recovery efficiency of linarin reached 81.55%. In addition, the DESs could be regenerated for extraction process, and macroporous resins could be further used for enrichment process with desired performance. This study provided an effective and convenient extraction and recovery method for linarin determination from CIF which was also applicable to other natural plants.
研究领域:
化学工程
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植物化学
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分析化学
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天然产物研究
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产品关联
DOI
高影响因子
RSC Advances
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2024
·Vol. 14
·期 26
·18126-18135
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IF 3.9
Junhai Liu,Xiaosha Guo,Qiaowei Miao,Xiaohui Ji,Yinku Liang,Tianjiao Tong
In this study, a response surface method (RSM) was used to optimise the ultrasonic-assisted deep eutectic solvent (DES) extraction of myricetin from myricetin leaves. The results demonstrated that the DES-5 (choline chloride–oxalic acid) system exhibited better extraction results than the other seven DESs prepared. The optimum extraction conditions for myricetin were a DES-5 system with 19% water content of DES, a liquid-to-solid ratio of 37 : 1 mL g−1, an extraction time of 45 min, and an extraction temperature of 72 °C. Under these conditions, the extraction amount of myricetin was 22.47 mg g−1. To optimise the extraction process, the crude myricetin extract was purified, and the optimal conditions were as follows: an AB-8 macroporous adsorption resin was used with an anhydrous ethanol desorption agent. The adsorption rate was 1 BV per h (bed volume per hour), the desorption rate was 1 BV per h, and the desorption capacity was 2 BV (bed volume). The antioxidant properties of the myricetin were also investigated. The results demonstrated that, with an increase in concentration, the scavenging rates of DPPH and ˙OH free radicals increased. Compared to Vc, myricetin had a better scavenging ability for DPPH free radicals, whereas purified myricetin had a better antioxidant effect. At the same concentration, the radical-scavenging rate of the ˙OH radical was slightly higher in myricetin purified by the macroporous adsorption resin than in Vc, and that of the unpurified myricetin was the smallest. Myricetin was purified using a macroporous adsorption resin to improve its antioxidant properties.
研究领域:
植物化学
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2020
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IF 4.244
Jie Shi,Yazhu Wang,Huanran Wei,Jiajun Hu,Min-Tian Gao
To reduce the production cost of bioethanol and improve the utilization rate of straw resources, more high value-added products have to be found and applied. In this study, tannins were considered as a high-value by-product. The extraction of condensed tannins from rice straw was studied, and the tannins were characterized. The crude tannins consisted mainly of tannins, phenolic acids and monosaccharides. The crude tannins were separated and purified with AB-8 macroporous resin and Sephadex LH-20. The matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) assay confirmed that catechin, epicatechin, epigallocatechin, epicatechin gallate and gallic acid were the major components in the purified tannins. The tannins exhibited strong inhibitory effects on growth of Staphylococcus aureus . Higher concentrations of crude tannins resulted in lower ethanol concentration and intercellular ATP content, and higher residual glucose. Moreover, the tannins could regulate morphology of biofilms. The images from atomic force microscopy and confocal laser scanning microscopy showed that cells hardly adhered to the plate surface, and large aggregations of cells were observed in culture medium containing tannins, and almost all cells were dead. This study provides the feasibility of tannins derived from rice straw as new antimicrobial agents with a different inhibitory mechanism.
研究领域:
生物化学
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微生物学
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生物技术
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药学
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DOI
高影响因子
Biomass Conversion and Biorefinery
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2023
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IF 4
Wen Yao,Zeng Xiang,Tan Hongyu,Liu Bing
To research the optimization of ultrasonic-assisted extraction of total flavonoids from Cortex Lycii and its biological activities. Box-Behnken design experiment was used to optimize the extraction process, and AB-8 macroporous resin was used for purification. Its biological activities were studied by the inhibition rate of α -glucosidase and α - amylase and the scavenging rate of DPPH and ABTS. The optimal extraction parameters of total flavonoids from Cortex Lycii were as follows: the extraction temperature was 63 ℃, the ethanol volume fraction was 71%, the solid–liquid ratio was 28 mL/g, the extraction time was 61 min. Under this optimal condition, the yield of total flavonoids was 1.674 mg/g, which was consistent with the predicted value of 1.637 mg/g, and was 5.85 times higher than that of the traditional water extraction method. The inhibitory rate (IC 50 ) of total flavonoids extract from Cortex Lycii on α -glucosidase was 3 μg/mL and on α -amylase was 5 μg/mL. The scavenging rate (IC 50 ) of DPPH radical was 0.648 mg/mL and the scavenging rate (IC 50 ) of ABTS radical IC 50 was 0.012 mg/mL. The optimized extraction process with response surface design was reliable, which could be used for the extraction of total flavonoids from Cortex Lycii . It has strong hypoglycemic and antioxidant activities and has a good prospect for future utilization and development.
研究领域:
药用植物学
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药学
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生物化学
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食品科学
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产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 7
·2200823
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IF 3.1
Gaodan Liu,Simin Feng,Minghui Sui,Bilian Chen,Peilong Sun
In this study, quartz sand with particularly sharp nanoscale edges acted like a nanoscale knife physically cut cells of Polygonatum cyrtonema Hua into nanosized particles and was synergized with natural deep eutectic solvent to extract steroidal saponins of Polygonatum cyrtonema Hua. The natural deep eutectic solvent (choline chloride-lactic acid)-synergistic quartz sand-assisted extraction was optimized using response surface methodology. The steroidal saponins purified with AB-8 macroporous resin were identified using ultra-high-performance liquid chromatography-triple time of flight mass spectrometry. The results showed that the experimental total saponins content value (36.97 ± 0.12 mg dioscin equivalent/g dry weight) at optimal extraction conditions with a temperature of 68°C, a rotational speed of 20 400 rpm, shear time of 4.3 min, the liquid-solid ratio of 38 ml/g, was close to the maximum possible theoretical value (36.64 mg dioscin equivalent/g dry weight). A total of 20 steroidal saponins were identified, among which the content of (25 R )-Kingianoside E was the highest (102.66 ± 3.47 mg/g). Furthermore, a new steroid saponin (3 β ,25 S )-26-( β -D-glucopyranosyloxy)-22-hydroxyfurost-5-en-3-yl 4- O - β -D-glucopyranosyl- β -D-galactopyranoside+Glc was found for the first time. These results revealed that natural deep eutectic solvent-synergistic quartz sand-assisted extraction was an efficient and green method to extract a variety of steroidal saponins.
研究领域:
药学化学
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分析化学
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植物科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）