COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
·
IF 5.2
Zhenliang Feng,Jufeng Huang,Hui Guo,Xinyue Zhang,Yuzhu Li,Bo Fang,Yan Li,Guang-Ling Song,Jie Liu
To repair the anti-corrosive coatings directly under seawater is an up-and-coming approach, which can effectively prolong the service life of coated steel structures once the coatings suffered from unexpected scratches. Inspired by the design of Band-Aid for the healing of wound, a magnetic “Band-Aid” (MB) combined with oleic acid modified Fe 3 O 4 nanoparticles (OMNPs) was designed to realize the in-situ repair of anti-corrosion coatings under seawater and the experimental results indicated that this coating repair method by MB incorporated with OMNPs modified epoxy binder is satisfying and effective when the addition of OMNPs epoxy binder was more than 1.0 wt%. Furthermore, the penetration of seawater in the vertical direction of the coating can be prevented by MB during the curing process of epoxy binder, the formation of permeation channels parallel to the coating can be blocked with the aid of OMNPs, which mainly based on the magnetic force between the MB and substrate steel, as well as the competitive-wetting and target-driving functions of OMNPs. The current work may provide a feasible approach to repair the organic anti-corrosion coating directly, which coated on the steel structures under seawater.
研究领域:
材料科学
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腐蚀与防护
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海洋工程
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产品关联
DOI
高影响因子
Microbial Cell Factories
·
2022
·Vol. 21
·期 1
·1-13
·
IF 6.352
Xin Qinglong,Chen Yudan,Chen Qianlin,Wang Bin,Pan Li
Background Bacillus amyloliquefaciens is generally recognized as food safe (GRAS) microbial host and important enzyme-producing strain in the industry. B.amyloliquefaciens LB1ba02 is a production strain suitable for secreting mesophilic α-amylase in the industry. Nevertheless, due to the low transformation efficiency and restriction-modification system, the development of its CRISPR tool lags far behind other species and strains from the genus Bacillus . This work was undertaken to develop a fast and efficient gene-editing tool in B.amyloliquefaciens LB1ba02. Results In this study, we fused the nuclease-deficient mutant Cas9n (D10A) of Cas9 with activation-induced cytidine deaminase (AID) and developed a fast and efficient base editing system for the first time in B. amyloliquefaciens LB1ba02. The system was verified by inactivating the pyrF gene coding orotidine 5'-phosphate decarboxylase and the mutant could grow normally on M9 medium supplemented with 5-fluoroorotic acid (5-FOA) and uridine (U). Our base editing system has a 6nt editing window consisting of an all-in-one temperature-sensitive plasmid that facilitates multiple rounds of genome engineering in B. amyloliquefaciens LB1ba02. The total editing efficiency of this method reached 100% and it achieved simultaneous editing of three loci with an efficiency of 53.3%. In addition, based on the base editing CRISPR/Cas9n-AID system, we also developed a single plasmid CRISPR/Cas9n system suitable for rapid gene knockout and integration. The knockout efficiency for a single gene reached 93%. Finally, we generated 4 genes ( aprE , nprE , wprA , and bamHIR ) mutant strain, LB1ba02△4. The mutant strain secreted 1.25-fold more α-amylase into the medium than the wild-type strain. Conclusions The CRISPR/Cas9n-AID and CRISPR/Cas9n systems developed in this work proved to be a fast and efficient genetic manipulation tool in a restriction-modification system and poorly transformable strain.
研究领域:
微生物学
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基因工程
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生物技术
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DOI
PubMed
高影响因子
Research
·
2025
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IF 10.7
Xiaolan Zhou,Xiaofeng Zhu,Weixu Wang,Jing Wang,Haimei Wen,Yuqi Zhao,Jiayu Zhang,Qiushi Xu,Zhaozhao Zhao,Ting Ni
Drug resistance to a single agent is common in cancer-targeted therapies, and rational drug combinations are a promising approach to overcome this challenge. Many Food and Drug Administration-approved drugs can induce cellular senescence, which possesses unique vulnerabilities and molecular signatures. However, there is limited analysis on the effect of the combination of cellular-senescence-inducing drugs and targeted therapy drugs. Here, we conducted a comprehensive evaluation of cellular senescence using 7 senescence-associated gene sets. We quantified the cellular senescence states of ~10,000 tumor samples from The Cancer Genome Atlas and examined their associations with targeted drug responses. Our analysis revealed that tumors with higher cellular senescence scores exhibited increased sensitivity to targeted drugs. As a proof of concept, we experimentally confirmed that etoposide-induced senescence sensitized lung cancer cells to 2 widely used targeted drugs, erlotinib and dasatinib. Furthermore, we identified multiple genes whose dependencies were associated with senescence status across ~1,000 cancer cell lines, suggesting that cellular senescence generates unique vulnerabilities for therapeutic exploitation. Our study provides a comprehensive overview of drug response related to cellular senescence and highlights the potential of combining senescence-inducing agents with targeted therapies to improve treatment outcomes in lung cancer, revealing novel applications of cellular senescence in targeted cancer therapies.
研究领域:
肿瘤学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
ADVANCED MATERIALS
·
2022
·Vol. 35
·期 10
·2208648
·
IF 32.086
Tao Sheng,Rui Jin,Changwei Yang,Ke Qiu,Mingyang Wang,Jiaqi Shi,Jingyu Zhang,Yuman Gao,Qing Wu,Xin Zhou,Hao Wang,Juan Zhang,Qin Fang,Neng Pan,Yanan Xue,Yue Wang,Rong Xiong,Fei Gao,Yuqi Zhang,Haojian Lu,Jicheng Yu,Zhen Gu
Timely administration of key medications toward patients with sudden diseases is critical to saving lives. However, slow transport of first-aid therapeutics and the potential absence of trained people for drug usage can lead to severe injuries or even death. Herein, an unmanned aerial vehicle (UAV)-mediated first-aid system for targeted delivery (uFAST) is developed. It allows unattended administration of emergency therapeutics-loaded transdermal microneedle (MN) patches toward patients to relieve symptoms by a contact-triggered microneedle applicator (CTMA). The implementability and safety of the uFAST for first aid is demonstrated in a severe hypoglycemic pig model by automatically delivering a glucagon patch with immediate and bioresponsive dual release modes. This platform technique may facilitate the development of UAV-mediated first-aid treatments for other sudden diseases.
研究领域:
生物医学工程
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急诊医学
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药学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Energy Chemistry
·
2023
·
IF 13.1
Xiaoye Liu,Xiangkun Kong,Wenyi Xiang,Yining Jiang,Bingqinq Xiong,Weiwei Ping,Changrong Xia,Daoming Huan,Chengwei Wang
Garnet-type Li 7 La 3 Zr 2 O 12 (LLZO) has high ionic conductivity and good compatibility with lithium metal. High-temperature processing has been proven an effective method to decrease the interface resistance of cathode|LLZO. However, its application is still hindered by the interlayer co-diffusion with the cathode and high sintering temperature (>1200 °C). In this work, a new garnet-type composite solid-state electrolyte (SSE) Li 6.54 La 2.96 Ba 0.04 Zr 1.5 Nb 0.5 O 12 -LiCoO 2 (LLBZNO-LCO) is firstly proposed to improve the chemical stability and electrochemical properties of garnet with high-temperature processing. Small doses of LCO (3%) can significantly decrease the LCO|SSE interface resistance from 121.2 to 10.1 Ω cm 2 , while the sintering temperature of garnet-type LLBZNO decreases from 1230 to 1000 °C. The all-solid-state battery based on the sintered LLBZNO-LCO SSE exhibits excellent cycling stability. Our approach achieves an enhanced LCO|SSE interface and an improved sintering activity of garnet SSE, which provides a new strategy for optimizing the comprehensive performance of garnet SSE.
研究领域:
材料科学
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电化学
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产品关联
DOI
高影响因子
NANO LETTERS
·
2022
·
IF 12.262
Ye Tian,Hanchuan Tang,Tianyu Kang,Xinyi Guo,Jiaxin Wang,Jianfeng Zang
Color vision deficiency (CVD) is a common ocular disorder affecting more than 300 million people on the earth. Although no clinical cure for the disorder currently exists, some specialized color filtering glasses/lenses based on dyes, metasurfaces, or nanocomposites have been employed for CVD management. However, as CVD patients usually diversify in their classification and severity, none of the current lenses provides a customized correction for various CVD patients, resulting in undesirable correction effects. Here, we present an inverse-designed approach for the precise correction of CVD. The wavelength shift of a patient’s abnormal cone photoreceptors was measured to inversely design the best blocking wavelength and blocking rate of the lens. Then the customized aid lenses were fabricated using silica-coated gold nanoparticles with appropriate sizes and concentrations, verified by the simulated color vision and human tests. This study demonstrates the potential of the inverse-designed aid lenses in precise color filtering and customized CVD management.
研究领域:
光学
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医学
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纳米科技
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DOI
高影响因子
BURNS
·
2021
·Vol. 48
·期 3
·585-594
·
IF 2.744
Huayu Zhang,Siyuan Yin,Jian Liu,Xia Li,Guoqi Cao,Yongqian Cao,Jiaxu Ma,Ru Song,Guang Zhang,Yibing Wang
Objective With the rapid development of networks in China, the Internet has become an important source of first aid information for scalds. As a country with the largest population, there are a large number of scald cases every year in China. The content analysis of websites aimed to assess the accuracy and quality of the information on scalds available on Chinese websites. Methods Using
研究领域:
公共卫生
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急诊医学
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健康传播
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信息科学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Tong Qi,Xiao Wang,Yijing Han,Chengqi He
The regeneration and functional rehabilitation of peripheral nerve defects remain major clinical challenges. Piezoelectric materials have the potential to restore both nerve morphology and function. Conventional grafts struggle to repair nerves with complex structures. To address this issue, band-aid–inspired tissue-adhesive membranes were designed to accommodate diverse nerve diameters and branching structures. Electrospinning was used to prepare a Janus membrane that served as a suture-free piezoelectric nerve repair membrane. The inner layer comprised oriented adhesive hydrogel fibers fabricated from gelatin methacryloyl and tannic acid, whereas the outer layer comprised piezoelectric silk fibroin fibers that provided barrier function and structural support. In vivo experiments demonstrated that this membrane streamlined surgery, enabled rapid and facile bridging of complex peripheral nerve defects, and significantly facilitated morphological and functional nerve recovery. This finding holds significant promise for advancing personalized, simplified, and more effective clinical management of peripheral nerve defects. Additionally, it is anticipated to treat defects in other tissues and organs, specifically those with irregular morphologies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Kexin Shen,Congcong Fan,Shengbo Ge,Lixia Wang,Wenqian Yin,Yaling Wang,Xiaoxia Wu,Xiangmeng Chen,Shun Wang,Meirong Song,Lei Jiang
Many diseases and pests are fond of the backs of leaves, making wraparound deposition essential for enhancing agrochemical utilization and minimizing environmental hazards. We present a superhydrophobic surface decorated with fluorinated-SiO2 nanoparticles on the adaxial (front) side, improving sprayed droplet wraparound behaviors and achieving a 10-fold increase in abaxial (backside) deposition without using an electrostatic sprayer. Solid–liquid contact electrification boosts the positive charge-to-mass ratio of rebound spraying from 17 to 454 nC g–1, with the abaxial surface acquiring opposite electric charges at kilovolt-level voltages. This intensified droplet-solid electrostatic attraction guides droplets to wrap around and deposit on the abaxial surface. Further, a homemade fluorinated superhydrophobic tube enhances spray charge and abaxial deposition density on superhydrophobic plant leaves by 47- and 5- times, respectively, compared to those obtained via direct spraying. This work will significantly improve agrochemical efficiency, reducing environmental risks in sustainable agriculture and related industries.
研究领域:
农业科学
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材料科学
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植物保护
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 29
·2100218
·
IF 18.808
Yun Sun,Jingling Liu,Hongyu Wang,Shanshan Li,Xueting Pan,Bolong Xu,Hailong Yang,Qingyuan Wu,Wenxuan Li,Xin Su,Zhijun Huang,Xindong Guo,Huiyu Liu
Microneedles (MNs) have attracted widespread scientific and industrial interest in the past decade as an efficient, painless, low-cost, and relatively safe transdermal drug delivery device. However, their drawbacks such as insufficient dose accuracy and limited penetration depth may limit the clinical applications. Here, a light-controlled liquid band-aid based on MNs is developed for antibacterial applications. Metal–organic framework-derived peroxidase-like nanozyme loaded in MNs can not only convert light energy into heat to enhance drug permeation but also decompose hydrogen peroxide into hydroxyl radicals for antibacteria. The heat generated by the nanozyme can facilitate MNs to melt and form a liquid band-aid, which is beneficial to insulate the wound from the surrounding bacterial environment. These studies in a Staphylococcus aureus -infected mice model also prove that this laser-triggered liquid band-aid can efficiently reduce skin inflammation and promote wound healing. Together, these results demonstrate that the rational design of MNs can enhance antibacterial and wound healing efficiency.
研究领域:
生物医学工程
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药学
·
伤口护理
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-9
·
IF 14.7
Lei Jingdan,Zhao Kunpeng,Liao Jincheng,Yang Shiqi,Zhang Ziming,Wei Tian-Ran,Qiu Pengfei,Zhu Min,Chen Lidong,Shi Xun
Grain boundary plays a vital role in thermoelectric transports, leading to distinct properties between single crystals and polycrystals. Manipulating the grain boundary to realize good thermoelectric properties in polycrystals similar as those of single crystals is a long-standing task, but it is quite challenging. Herein, we develop a liquid-phase sintering strategy to successfully introduce Mg 2 Cu nano-sintering-aid into the grain boundaries of Mg 3 (Bi, Sb) 2 -based materials. The nano-aid helps to enlarge the average grain size to 23.7 μm and effectively scatter phonons, leading to excellent electrical transports similar as those of single crystals and ultralow lattice thermal conductivity as well as exceptional thermoelectric figure of merit (1.5 at 500 K) and conversion efficiency (7.4% under temperature difference of 207 K). This work provides a simple but effective strategy for the fabrication of high-performance polycrystals for large-scale applications.
研究领域:
材料科学
·
物理学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF REFRACTORY METALS & HARD MATERIALS
·
2018
·
IF 2.606
Te Qi,Zhihao Zhao,Yongqiang Sun,Hangyuan Zhao,Xiaolei Li,Huiming Ji
Porous Si 3 N 4 ceramics with a unique interlocking microstructure have been fabricated by pressureless liquid-phase sintering with YbF 3 and CaF 2 sintering additives at a relatively low temperature (1550 °C). Phase transition and mass diffusion in the low-temperature sintering process are facilitated by YbF 3 and CaF 2 producing plenty of liquid phase owing to their extremely low melting points. As the content of YbF 3 increases from 8.0 wt% to 9.5 wt% and the CaF 2 decreases from 2.0 wt% to 0.5 wt%, the conversion rate of the phase transition from α-Si 3 N 4 to β-Si 3 N 4 increases from 71.1% to 81.2% and the largest average aspect ratio of the β-Si 3 N 4 grains reaches 10.05. At the same time, the porosity declines from 40.4% to 34.9% and the flexural strength increases from 168.5 MPa to 232.1 MPa.
研究领域:
材料科学
·
陶瓷工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）