MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Jia Wei,Yaoqi Wang,Jiaxue Gao,Zhenshengnan Li,Renzhu Pang,Tianchao Zhai,Yuan Ma,Zhenxin Wang,Xianying Meng
Qualitative and quantitative analysis of carcinogenic mutations in circulating tumor DNA (ctDNA) plays a unique role in the early screening and precise diagnosis of cancer. Herein, we introduced an electrochemical-enrichment assisted amplification refractory mutation system-quantitative real-time PCR (EC-ARMS-qPCR) assay to detect the BRAF V600E carcinogenic mutation of thyroid cancer in plasma samples. Cell-free DNA (cfDNA) were firstly enriched by an electric field-driven adsorption and desorption process, and then ctDNA in cfDNA were analyzed by a designed allele-specific qPCR assay. The EC-ARMS-qPCR assay provides good sensitivity and specificity for the identification of single nucleotide polymorphisms (SNPs) of BRAF V600E mutant ctDNA in a complex system, which can detect as low as 2 BRAF V600E mutant copies in Tris buffer and 25 BRAF V600E mutant copies in Tris buffer containing 50% plasma with a allele frequency of 0.01% (i.e., mutant-to-wild-type ratio of 1: 9999). The assay was assessed in plasma samples of patients with papillary thyroid cancer (PTC) and benign thyroid nodule (TN). The EC-ARMS-qPCR assay detected 40.74% and 10.00% BRAF V600E mutation ctDNA in plasma samples of 54 PTC patients and 20 TN patients, respectively. The overall concordance between BRAF V600E mutant ctDNA by EC-ARMS-qPCR assay and BRAF V600E mutant of matched pathological tissue genes by fine needle aspiration biopsy (FNAB) is 73.08%, suggesting that the EC-ARMS-qPCR assay is appropriate for detecting carcinogenic mutations in liquid biopsy samples.
研究领域:
肿瘤学
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分子生物学
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医学遗传学
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临床诊断
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产品关联
DOI
高影响因子
Biomolecules
·
2025
·Vol. 15
·期 3
·315
·
IF 4.8
Dongxia Pan,Mukaddas Mijit,Hui Wang,Chaoqun Sun,Bantan Pingcuo,Zhixue Yu,Benhai Xiong,Xiangfang Tang
The A-to-G mutation (FecB) in theBMPR1Bgene is strongly linked to fertility in sheep, significantly increasing ovulation rates and litter sizes compared to wild-type populations. The rapid and reliable screening of theFecBgene is therefore critical for advancing sheep breeding programs. This study aimed to develop a fast and accurate method for detecting theFecBmutation and genotyping the gene to enhance sheep reproduction and productivity. To achieve this, we integrated the CRISPR-Cas12a system with an optimized amplification refractory mutation system (ARMS). A similar DNA origami technique-based fluorescence reporter nanotree structure was synthesized using gold nanomagnetic beads as carriers to amplify the fluorescence signal further. The resulting biosensing platform, termed CRISPR-ARMS, demonstrated excellent sensitivity for detectingFecBmutations, with a detection limit as low as 0.02 pmol. Therefore, this innovative approach shows great promise for single-base mutation detection and represents a pioneering tool for high-yield genetic screening.
研究领域:
分子生物学
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遗传学
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动物科学
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DOI
PubMed
高影响因子
CRIME LAW AND SOCIAL CHANGE
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2000
·Vol. 33
·期 1
·1-52
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IF 0.197
Phythian Mark
This article considers the principal changes that haveoccurred in the illicit arms trade across the Cold Warand post-Cold War periods. It discusses the changednature of demand and the sources and means ofillicitly supplying arms to areas of conflict. Througha number of case studies it highlights the declininguse of illicit arms supply as a foreign policy tool,and the extent to which involvement in the trade isnow determined more by profit than policyconsiderations, with all the implications thesechanges have for control initiatives.
研究领域:
国际关系
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安全研究
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军控
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政治经济学
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DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2025
·
IF 3.2
Huishan Chen,Xiaoyi Sun,Ning Qing,Liuyan Tang
Hydrophobically modified ethoxylated urethane (HEUR), a typical polymer associative thickener, is extensively applied in aqueous coatings, textile printing, and resin coatings to regulate the rheological properties of the system. However, conventional branched HEURs face two critical limitations: (1) the risk of excessive cross-linking during the chain-expansion stage due to high monomer reactivity; (2) the phase-separation phenomenon in some branched HEUR aqueous solutions upon standing. To overcome these challenges, we designed and synthesized a polyhydroxy hyperbranched structure (PU-OH 3-6 ). This structure features two main advantages: a larger central backbone and a greater spacing between active groups (OH). The hyperbranched HEUR 3-6 synthesized using PU-OH 3-6 as a branched-type chain extender is more controllable and viable in the production process. Moreover, various testing methods were employed to assess the thickening performance, salt resistance, and water retention of HEUR in a pure-water system. The results indicated that the branched structures of HEUR 3-6 have better thickening properties compared with the previously reported HEUR with a linear structure (HEUR L ), and branched HEUR with using gamma-triol (HEUR GI ), pentaerythritol (HEUR P ), and xylitol (HEUR X ) to expand the chain. Among them, HEUR 5 demonstrated the best thickening effect in pure-water systems, while in salt-containing solutions, HEUR 6 with a six-branched degree showed more pronounced anti-electrolyte properties.
研究领域:
高分子科学
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材料工程
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化学合成
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流变学
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涂料技术
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产品关联
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
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2015
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IF 2.358
Jingcheng Liu,Ren Liu,Xiaopeng Zhang,Zhiquan Li,Hongtao Tang,Xiaoya Liu
A series of multi-arms cardanol-based acrylates (CGHA, CDHA, CTHA) were synthesized using renewable cardanols and bio-based polyols (glycerol, diglycerol, tripilyglycerol) as precursors. The structures and molecular weights of the cardanol-based acrylates were characterized by FT-IR, 1 H NMR and GPC , respectively. The synthesized multi-arms cardanol-based acrylates were used to produce UV-curable films and coatings. High mechanical strength as well as good biodegradability of the UV-cured films was obtained. The important coating film properties, such as hardness, gloss and adhesion were also investigated. The results showed that the coatings with high bio-based content exhibit good adhesion, gloss and acid-resistance, which is comparable to the soybean oil (AESO)-based and polyester acrylate.
研究领域:
材料科学
·
可再生资源化学
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DOI
高影响因子
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
·
2019
·Vol. 38
·期 1
·1-16
·
IF 5.646
Xia Mao,Luo Dongjun,Dong Jie,Zheng Meihong,Meng Gang,Wu Junhua,Wei Jiwu
Background Replication-competent oncolytic viruses (OVs) have been proven to be a potent anticancer weapon for clinical therapy. The preexisting neutralizing antibody in patients is a big challenge for oncolytic efficacy of OVs. Graphene oxide sheets (GOS) possess excellent biological compatibility and are easy to decorate for targeted delivery.Methods We generated PEI-GOS-PEG-FA (Polyethyleneimine-Graphene oxide sheets-Polyethylene glycol-Folic acid). After intravenous injection, the distribution of PEI-GOS-PEG-FA in tumor-bearing mice was visualized by the IVIS Lumina XR system. Then, the oncolytic measles virus (MV-Edm) was coated with PEI-GOS-PEG-FA to form a viral-GOS complex (GOS/MV-Edm). The oncolytic effects of GOS/MV-Edm were investigated both in vitro and in vivo.Results GOS/MV-Edm exhibited higher infectivity and enhanced oncolysis. In tumor-bearing mice, GOS/MV-Edm had significantly elevated viral replication within the tumor mass, and achieved an improved antitumor effect. Then, we confirmed that GOS/MV-Edm entered cancer cells via the folate receptor instead of CD46, a natural cognate receptor of MV-Edm. GOS/MV-Edm remained the infectivity in murine cells that lack CD46. Finally, we found that GOS/MV-Edm was effectively protected from neutralization in the presence of antiserum both in vitro and in vivo. In passively antiserum immunized tumor-bearing mice, the survival was remarkably improved with intravenous injection of GOS/MV-Edm.Conclusion Our findings demonstrate that GOS/MV-Edm displays significantly elevated viral replication within the tumor mass, leading to an improved antitumor effect in solid tumor mouse model. Our study provided a novel strategy to arm OVs for more efficient cancer therapy. That may become a promising therapeutic strategy for cancer patients.
研究领域:
肿瘤学
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病毒学
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纳米技术
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癌症治疗
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DOI
PubMed
高影响因子
EMBO JOURNAL
·
2026
·Vol. 45
·期 4
·1109-1135
·
IF 8.4
Jiang Wenli,Ye Jin,Chen Jiasheng,Wang Xinyu,Li Yahong,Li Jianchao,Mei Yide,Lyu Yanlu,Hu Wei,Wang Chao
ARMS (ankyrin repeat-rich membrane spanning) is a scaffold protein essential for neurotrophic signaling, synaptic development, and cytoskeletal remodeling. Despite its central role in neuronal function, how ARMS is regulated at the molecular level remains poorly understood. Here, we identify GABARAP, an Atg8-family autophagy adaptor, as a novel ARMS-binding protein that directly interacts with its N-terminal ankyrin repeats. We present the crystal structure of the ARMS-GABARAP complex, revealing an atypical interaction mode distinct from canonical LIR-dependent Atg8 interactions. Remarkably, ARMS specifically binds to the GABARAP subfamily of Atg8 proteins, setting it apart from the LC3 subfamily. Functional analysis demonstrates that GABARAP negatively regulates ARMS-mediated dendritic spine development and maturation in hippocampal neurons. Additionally, disrupting the ARMS-GABARAP complex using ankyrin-derived peptides alters ARMS subcellular localization, increasing its accumulation in the soma of neurons. Collectively, our findings uncover a novel ARMS-GABARAP interaction mechanism, establish the regulatory role of this complex in neuronal protein homeostasis, and suggest potential therapeutic strategies for targeting scaffold protein interactions in neurodevelopmental and neurodegenerative disorders.
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DOI
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2013
·Vol. 61
·期 3
·341-344
·
IF 0.879
Dong An,Zhiwen Ye
According to active groups synergetic catalysis principle, a novel phosphodiester receptor 1-( N -guanidinoethyl)-4-( N -hydroxyethyl)-piperazidine hydrochloride was firstly synthesized and the preliminary studies of its DNA cleavage activity. It is characterized and confirmed by methods as 1 H NMR and 13C NMR. A “couple hardness with softness” piperazidine is designed to connect guanidinium group and hydroxyl group; The reserch was showed that the best cleaving conditions was 7.2 in pH; Cleaving DNA by the compound reaction was oxidation process that were proved through the free radicals quenches experiments; The compound can cleaved the pUC 19 DNA by phospholipid transferance was proved by BDNPP.
研究领域:
化学
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生物化学
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF POLYMER SCIENCE
·
2023
·Vol. 61
·期 12
·1171-1182
·
IF 3.4
Yue Meng,Hong Zhai,Ziting Zhou,Xiaoying Wang,Jiandong Han,WenJuan Feng,Yuxin Huang,Yuan Wang,Ying Bai,Jing Zhou,Daping Quan
The development of artificial organs and implants demands new materials with both processability and functionality. A series of star like poly(CL- co -TOSUO) copolymers with different molecular weights are synthesized via ring-opening polymerization. The polymers are modified with norbornene end groups for rapid photo-crosslinking. Due to the random insertion of 1,4,8-trioxaspiro-[4,6]-9-undecanone and well-constructed permanent chemical network, the photo-crosslinked elastomers are more flexible and resilient than conventional poly(ε-caprolactone), with 84% resilience above the melting point. Their thermal and mechanical properties can be fine-tuned by varying the molecular weights of the precursors. The polymer can be fabricated into complex shapes by digital light processing 3D printing. Combined with good cytocompatibility and degradability, the highly flexible elastomers show great potential for tissue engineering. Graphical
研究领域:
材料科学
·
生物医学工程
·
组织工程
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
·
IF 5.4
Chong Wang,Rong-Chao Xue,Ze-Zhong Lin,Xing-Ping Zhang,Pei-Yao Chen,Yu Chen,Hai-Chao Li
Flexible plasticized poly(vinyl chloride) (PPVC) is widely used in medical devices; however, achieving persistent antibiofouling functionality after significant mechanical deformation and long-term water exposure remains a critical challenge. In this work, we introduce amphiphilic heterografted multi-arm star copolymers, polyethylenimine (PEI) cores functionalized with polyethylene glycol (PEG) and poly(ε-caprolactone) (PCL) arms (denoted as PEI-g-PEG-PCLs), as multifunctional additives for modifying PPVC. Dynamic light scattering (DLS) and optical transmittance measurements confirmed the formation of PEI-g-PEG-PCL micelles within the PPVC matrix. The incorporation of PEI-g-PEG-PCLs improved not only the mechanical and thermal properties of PPVC but also its surface hydrophilicity. The resulting composites demonstrated exceptional resistance to biofouling, reducing protein adsorption by 96.2–97.3 % for bovine serum albumin and 98.0–99.8 % for lysozyme, and lowering bacterial adhesion rates to 11 % for Escherichia coli and 19 % for Staphylococcus aureus. Furthermore, platelet adhesion and hemolysis assays confirmed the biocompatibility of the PEI-g-PEG-PCL/PPVC composites. Notably, even after undergoing three cycles of 50 % strain, the optimal PEI-g-PEG-PCL/PPVC composite retained over 91 % of its initial anti-protein fouling performance. Long-term immersion in physiological conditions (28 days) further demonstrated its durability, with more than 92 % of the original antifouling efficacy maintained. The multi-arm star topology accompanied with the long PCL arm is proposed to resolve the “entanglement–migration paradox” commonly encountered in linear or less branched polymer additives. This study presents a molecular engineering strategy for developing durable antibiofouling polymers capable of withstanding both mechanical stress and prolonged aqueous exposure in medical applications.
研究领域:
高分子科学
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生物医学工程
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材料科学
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抗污材料
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医疗器械
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS CHEMISTRY
·
2011
·Vol. 21
·期 28
·10390-10398
·
IF 5.101
Kunmin Yang,Hui Liang,Jiang Lu
A well-defined multifunctional star polymer consisting of reactive and thermoresponsive poly(N-isopropylacrylamide-co-acrolein) arms and an aluminum tris(8-hydroxyquinoline) (Alq3)-bearing fluorescently labeled core was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization using the “arm-first” method. Acrolein, a commercially available aldehyde-bearing monomer, was copolymerized with N-isopropylacrylamide (NIPAM) via RAFT technique in a controlled way to give well-defined linear trithiocarbonate terminated poly(NIPAM-co-acrolein) macro-RAFT agent. The preformed linear macro-RAFT agent was then cross-linked by a tri-vinyl Alq3-containing cross-linker (Alq3 cross-linker) in conjunction with N,N′-methylenebisacrylamide to give the desired reactive, thermoresponsive, and fluorescently labeled star polymer. The resultant star polymer exhibits a lower critical solution temperature at ∼29.8 °C in water, and displays intense greenish-yellow fluorescence with maximum emission peak around 520 nm in both organic solvent (i.e., THF) and water. Moreover, the highly reactive aldehyde functions in the arms of the star polymer could provide the key intermediates to conjugate biomolecules, and the conjugation with an aminooxy-functionalized BSA model protein was also demonstrated as an example of how the novel star polymer can be utilized to conjugate bioactive molecules, constructing fluorescently labeled “smart” polymer-protein conjugates.
研究领域:
高分子化学
·
生物材料
·
生物化学
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DOI
高影响因子
DYES AND PIGMENTS
·
2017
·
IF 3.473
Cuixia Yuan,Wenqing Liu,Minmin Shi,Shuixing Li,Yuzhou Wang,Huanle Chen,Chang-Zhi Li,Hongzheng Chen
In this paper, a non-fullerene electron acceptor , SF(DPPFB) 4 , which owns a spirobifluorene core and four diketopyrrolopyrrole (DPP) arms end capped by 4-fluorobenzene, is designed and synthesized for solution-processable organic solar cells (OSCs). SF(DPPFB) 4 shows similar absorption bands as those of its non-fluorinated parent compound, SF(DPPB) 4 . However, the terminal fluorine atoms reduce the energy levels of SF(DPPFB) 4 , especially, its lowest unoccupied molecular orbital level decreases by 0.04 eV than that of SF(DPPB) 4 , which enlarges the energy offset between the electron donor and acceptor, favorable for the dissociation of excitons in OSCs. Moreover, the fluorination improves the electron mobility of SF(DPPFB) 4 . Thus, the OSCs with poly(3-hexylthiophene) as the electron donor and SF(DPPFB) 4 as the electron acceptor can provide a maximum power conversion efficiency of 4.42%, with a lifted short-circuit current ( J sc ) of 8.48 mA cm −2 .
研究领域:
材料科学
·
能源科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）