INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2015
·
IF 3.082
Rui Cai,Yahong Yuan,Zhouli Wang,Chunfeng Guo,Bin Liu,Chunqing Pan,Laping Liu,Tianli Yue
Alicyclobacillus acidoterrestris can survive the pasteurization process, multiply in pasteurized juices and produce guaiacol which causes medicinal or antiseptic off-flavors. Chemical preservatives have the potential to suppress outgrowth of surviving populations during subsequent storage of fruit juices. In the present study, the individual effects of potassium sorbate , sodium benzoate , potassium metabisulfite , dehydroacetic acid , ethyl 4-hydroxybenzoate, cinnamic acid and ε-polylysine on A. acidoterrestris growth and guaiacol production were firstly evaluated in a laboratory medium. Of the seven preservatives investigated, only dehydroacetic acid, cinnamic acid and ε-polylysine were effective both in controlling growth and guaiacol formation by A. acidoterrestris . Then, these three antimicrobials were applied to apple juice. Through the addition of 270 mg/L dehydroacetic acid, 108 mg/L cinnamic acid or 100 mg/L ε-polylysine, the A. acidoterrestris counts were reduced by 3.43, 3.17 and 4.78 log colony forming unit (CFU)/mL, respectively, and no guaiacol was detected after 14 days of storage. Sensory evaluation revealed that the addition of these three preservatives did not affect the organoleptic properties of the apple juice. Results obtained in this paper could be very useful for a better control of A. acidoterrestris -related spoilage in the fruit juice/beverage industry.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2015
·
IF 3.082
Rui Cai,Yahong Yuan,Zhouli Wang,Chunfeng Guo,Bin Liu,Laping Liu,Yutang Wang,Tianli Yue
Alicyclobacillus acidoterrestris has recently received much attention due to its implication in the spoilage of pasteurized fruit juices, which was manifested by the production of guaiacol. Vanillic acid and vanillin have been accepted as the biochemical precursors of guaiacol in fruit juices. The purpose of this study was to try to find other precursors and elucidate details about the conversion of vanillic acid and vanillin to guaiacol by A . acidoterrestris . Four potential substrates including ferulic acid, catechol, phenylalanine and tyrosine were analyzed, but they could not be metabolized to guaiacol by all the thirty A . acidoterrestris strains tested. Resting cell studies and enzyme assays demonstrated that vanillin was reduced to vanillyl alcohol by NADPH-dependent vanillin reductase and oxidized to vanillic acid by NAD(P) + -dependent vanillin dehydrogenases in A . acidoterrestris DSM 3923. Vanillic acid underwent a nonoxidative decarboxylation to guaiacol. The reversible vanillic acid decarboxylase involved was oxygen insensitive and pyridine nucleotide-independent.
研究领域:
微生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2021
·
IF 5.64
Fan Qiuxia,Liu Cheng,Gao Zhenpeng,Hu Zhongqiu,Wang Zhouli,Xiao Jianbo,Yuan Yahong,Yue Tianli
Alicyclobacillus acidoterrestris (A. acidoterrestris), a spore-forming bacterium, has become a main challenge and concern for the juices and acid beverage industry across the world due to its thermo-acidophilic characteristic. Thymoquinone (TQ) is one of the active components derived from Nigella sativa seeds. The objective of this study was to investigate antibacterial activity and associated molecular mechanism of TQ against A. acidoterrestris vegetative cells, and to evaluate effects of TQ on A. acidoterrestris spores and biofilms formed on polystyrene and stainless steel surfaces. Minimum inhibitory concentrations of TQ against five tested A. acidoterrestris strains ranged from 32 to 64 μg/mL. TQ could destroy bacterial cell morphology and membrane integrity in a concentration-dependent manner. Field-emission scanning electron microscopy observation showed that TQ caused abnormal morphology of spores and thus exerted a killing effect on spores. Moreover, TQ was effective in inactivating and removing A. acidoterrestris mature biofilms. These findings indicated that TQ is promising as a new alternative to control A. acidoterrestris and thereby reduce associated contamination and deterioration in the juice and acid beverage industry.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 17
·3276
·
IF 5.2
Siqi Zhao,Yanzi Nan,Runyu Yao,Langhong Wang,Xinan Zeng,Rana Muhammad Aadil,Muhammad Asim Shabbir
The aim of this research was to investigate the antimicrobial characteristics and mechanism of hesperetin againstAlicyclobacillus acidoterrestrisvegetative cells. The results presented show that hesperetin had effective antimicrobial activity onAlicyclobacillus acidoterrestrisvegetative cells, minimum inhibition concentration (MIC) of 0.0625 g/L, and minimum bacterial concentration (MBC) greater than 2 g/L. Moreover, treatment of hesperetin caused significant damage to cell integrity, preventing the growth ofAlicyclobacillus acidoterrestrisvegetative cells, enhancing the leakage of nucleic acid and proteins, and destroying the vegetative cell morphology. To further investigate the mechanism, transcriptomic analysis was carried out, and 3056 differentially expressed genes (DEGs) were detected. Gene ontology (GO) enrichment analysis revealed that hesperetin inhibitsAlicyclobacillus acidoterrestrisby affecting the intracellular nitrogen metabolism and amino acid metabolism. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis explained that hesperetin was also able to prevent the growth ofAlicyclobacillus acidoterrestrisby affecting the processes of nutrient transport, energy metabolism, and flagella motility. These results provide new insights into the antimicrobial effects and mechanism of hesperetin againstAlicyclobacillus acidoterrestris, which provides a new method for inactiveAlicyclobacillus acidoterrestrisin the juice industry.Keywords:Alicyclobacillus acidoterrestris;hesperetin;antibacterial activity;antibacterial mechanism;transcriptomic analysis
研究领域:
微生物学
·
分子生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Rui Cai,Mingquan Zhang,Lu Cui,Yahong Yuan,Yifei Yang,Zhouli Wang,Tianli Yue
Alicyclobacillus acidoterrestris has received much attention due to its unique thermo-acidophilic property and implication in the spoilage of pasteurized juices. The aim of this study was to investigate the antibacterial characteristics and mechanisms of thymol against A. acidoterrestris vegetative cells and spores. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of thymol against vegetative cells were both 0.25 mg/mL. MIC of thymol against spores was 0.5 mg/mL, and the MBC was greater than 1.0 mg/mL. Thymol could effectively inhibit A. acidoterrestris growth, spore germination and guaiacol production. When treated by thymol, the leakage of nucleic acids and proteins from A. acidoterrestris cells and spores increased significantly. SDS-PAGE investigation of bacterial proteins showed that the loss of soluble proteins was obvious as well. Scanning electron microscopy observation exhibited that A. acidoterrestris cells or spores treated with thymol had a severe morphological damage. These results demonstrated that thymol exerted its good antibacterial activities by destroying the integrity of the cell and spore. This study provides a new method for the inactivation of A. acidoterrestris in fruit juice.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
高影响因子
ANALYST
·
2014
·Vol. 139
·期 17
·4315-4321
·
IF 3.906
Tao Liu,Xiao Zhang,Wenxin Zhu,Wei Liu,Daohong Zhang,Jianlong Wang
The rapid and sensitive detection of Alicyclobacillus acidoterrestris (AA) has become very important due to the frequent occurrence of fruit juice spoilage by AA. In the present study, using guaiacol, both as the metabolic product of AA related to its concentration and as a green colorimetric substrate of G-quadruplex DNAzyme, a novel G-quadruplex DNAzyme-based colorimetric method for a rapid detection of AA has been developed for the first time. Under optimal conditions, AA has been successfully detected in the concentration range of 102–105 cfu mL−1 with a detection limit of 85 cfu mL−1. The recoveries ranging from 71.8% to 115.7% with relative standard deviation from 1.2% to 6.6% in spiked apple and orange juice samples were obtained. Results demonstrate that the sensitivity and precision of the developed method is comparable with most other analytical methods and is prominently rapid than them. We believe that the work provides a novel and effective approach and is beneficial for monitoring and reducing the risk of AA contaminations during the process of fruit juice production.
研究领域:
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Hang Jia,Yaping Zhang,Qi Guo,Xuejun Zeng,Yahong Yuan,Zhouli Wang,Zhenpeng Gao,Tianli Yue
In this work, polyacrylic acid modified ferroferric oxide (Fe 3 O 4 -PAA) was synthesized via a one-step hydrothermal method. The magnetic nanospheres modified with carboxyl groups were combined with epsilon-polylysine (EPL) via an EDC/NHS coupling reaction to obtain Fe 3 O 4 -PAA-EPL nanospheres. Fe 3 O 4 -PAA-EPL was employed as an antibacterial agent against Alicyclobacillus acidoterrestris and characterized by XRD, FTIR, XPS, VSM, SEM and TEM techniques. Experimental results showed the minimum inhibition concentration (MIC) of Fe 3 O 4 -PAA-EPL against A. acidoterrestris was 1.25 mg mL −1 . Furthermore, A. acidoterrestris treated with Fe 3 O 4 -PAA-EPL nanospheres obviously lysed. Morphological analysis of bacteria supported by SEM indicated that the cell membrane of A. acidoterrestris was damaged, revealing that Fe 3 O 4 -PAA-EPL is an effective antibacterial agent. Additionally, the nanospheres with excellent magnetism can be simply separated from a reaction system via an external magnet. The construction of magnetic nanospheres with satisfactory antibacterial activity provides an effective and new method to control A. acidoterrestris .
研究领域:
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Hang Jia,Xuejun Zeng,Rui Cai,Zhouli Wang,Yahong Yuan,Tianli Yue
The risk of fruit juice contamination caused by microorganisms, especially Alicyclobacillus acidoterrestris, has been reported worldwide. To develop cost-effective control methods, in this work, flower-like magnetic molybdenum disulfide (Fe3O4@MoS2) nanoparticles (NPs) were fabricated by a facile two-step hydrothermal method. After further modifying polyacrylic acid (PAA) on the surface of the NPs, epsilon-polylysine (EPL) was immobilized via N-(3-dimethylaminopropyl)-N-carbodiimide hydrochloride/N-hydroxysuccinimide coupling reaction to obtain the Fe3O4@MoS2@PAA–EPL nanocomposites. Antibacterial results exhibited that the synthesized nanocomposites showed effective antibacterial activity against A. acidoterrestris with a minimum inhibitory concentration of 0.31 mg mL–1. Investigation on the antibacterial mechanism revealed that the presence of nanocomposites caused damage and disruption of the bacterial membrane through dent formation, resulting in the leakage of intracellular protein. Moreover, the activity of dehydrogenase enzymes was inhibited with the treatment of Fe3O4@MoS2@PAA–EPL, causing the reduction of metabolic activity and adenosine triphosphate levels in bacteria. Simultaneously, the presence of nanocomposites improved intracellular reactive oxygen species levels, and this disrupted the antioxidant defense system and caused oxidative damage to bacteria. Furthermore, Fe3O4@MoS2@PAA–EPL nanocomposites were confirmed to possess satisfactory biocompatibility by performing in vitro cytotoxicity and in vivo acute toxicity experiments. The aim of this research was to develop a new pathway for the inhibition of A. acidoterrestris in the juice industry.
研究领域:
食品科学
·
微生物学
·
纳米材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2024
·
IF 5
Hao Ding,Tiecheng Wang,Yuxiang Zhang,Chunfeng Guo,Kaiyu Shi,Ivan Kurtovic,Yahong Yuan,Tianli Yue
As spores of Alicyclobacillus acidoterrestris can survive traditional pasteurization , this organism has been suggested as a target bacterium in the fruit juice industry . This study aimed to investigate the inactivation effect of cold plasma on A. acidoterrestris spores and the mechanism behind the inactivation. The inactivation effect was detected by the plate count method and described by kinetic models. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), the detection of dipicolinic acid (DPA) release and heat resistance detection, the detection and scavenging experiment of reactive species, and cryo-scanning electron microscopy were used to explore the mechanism of cold plasma inactivation of A. acidoterrestris . The results showed that cold plasma can effectively inactivate A. acidoterrestris spores in saline with a 3.0 ± 0.3 and 4.4 ± 0.8 log reduction in CFU/mL, for 9 and 18 min, respectively. The higher the voltage and the longer the treatment time, the stronger the overall inactivation effect. However, a lower gas flow rate may increase the probability of spore contact with reactive species, resulting in better inactivation results. The biphasic model fits the survival curves better than the Weibull model. SEM and TEM revealed that cold plasma treatment can cause varying degrees of damage to the morphology and structure of A. acidoterrestris spores, with at least 50 % sustaining severe morphological and structural damage. The DPA release and heat resistance detection showed that A. acidoterrestris spores did not germinate but died directly during the cold plasma treatment. 1 O 2 plays the most important role in the inactivation, while O 3 , H 2 O 2 and NO 3 − may also be responsible for inactivation. Cold plasma treatment for 1 min reduced A. acidoterrestris spores in apple juice by 0.4 ± 0.0 log, comparable to a 12-min heat treatment at 95 °C. However, as the treatment time increased, the survival curve exhibited a significant tailing phenomenon, which was most likely caused by the various compounds in apple juice that can react with reactive species and exert a physical shielding effect on spores. Higher input power and higher gas flow rate resulted in more complete inactivation of A. acidoterrestris spores in apple juice. What's more, the high inactivation efficiency in saline indicates the cold plasma device provides a promising alternative for controlling A. acidoterrestris spores during apple washing. Overall, our study provides adequate data support and a theoretical basis for using cold plasma to inactivate A. acidoterrestris spores in the food industry.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2018
·Vol. 54
·期 5
·1697-1705
·
IF 2.383
Rui Cai,Miao Miao,Tianli Yue,Yijun Zhang,Lu Cui,Zhouli Wang,Yahong Yuan
Summary The aim of this study was to investigate the antibacterial activity and mechanism of cinnamic acid and chlorogenic acid against Alicyclobacillus acidoterrestris . Minimum inhibitory concentrations of cinnamic acid and chlorogenic acid were 0.375 and 2.0 mg mL −1 , and the minimum bactericidal concentrations were 0.50 and 4.0 mg mL −1 , respectively. The apple juice ingredients had little influence on the inactivation of A. acidoterrestris . After treatment with cinnamic acid and chlorogenic acid, the morphology of A. acidoterrestris cells were severely destroyed; the leakage of nucleic acids and proteins increased significantly. SDS-PAGE investigation of bacterial proteins proved that the loss of soluble proteins was obvious as well. These results demonstrated that cinnamic acid and chlorogenic acid exerted their antibacterial activity mainly by an action mode of membrane disruption. This study provides an alternative method for the control of A. acidoterrestris -related spoilage in the fruit juice/beverage industry.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2024
·
IF 5.3
Xiaodan Guo,Lei Zhang,Xinian Li,Xiaojing Ren,Jian Yu,Tianli Yue,Fangyu Long
High hydrostatic pressure (HHP) is widely used to inactivate microorganisms in food, but achieving effective spore inactivation by conventional HHP remains challenging due to their resistance. The present study compared the effectiveness of continuous, intermittent and induced inactivation HHP techniques for inactivation of Alicyclobacillus acidoterrestris spores. Under optimal induced inactivation conditions (200 MPa/4 cycles, 600 MPa/2 cycles), reductions of 0.79 log CFU/mL for the highly resistant DSM3922 spores and 1.94 log CFU/mL for the less resistant DSM3924 spores were achieved. Mechanistic investigations revealed that: (Ⅰ) structural damage, including breakage, wrinkling, and concavity, was induced in spores post-HHP treatment; (Ⅱ) pulsation forces generated by repeated pressurization and depressurization during induced inactivation HHP reduced the D90 values of the spore aggregates to 99.37 μm for DSM3922 and 76.49 μm for DSM3924, enhancing the inactivation efficacy; (Ⅲ) the inner membrane (IM) and cortex were severely impaired, while nucleic acid and protein leakage from spores significantly increased after HHPs. To further enhance spore inactivation, Tea Tree, Nutmeg, Eucalyptus, and Litsea cubeba essential oils (EOs) were selected from twelve EOs as safe and effective antimicrobial substances that synergize with HHP. Combining 5% Litsea cubeba EO with HHP (200 MPa/4 cycles, 600 MPa/2 cycles) resulted in reductions of 2.57 log CFU/mL for DSM3922 and 5.08 log CFU/mL for DSM3924. These findings suggest that the combination of HHP and EOs could be a promising approach for inactivating A. acidoterrestris spores.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2025
·
IF 6.8
Xuemei Meng,Tong Zhang,Jun Li,Xiya Cao,Linan Duan,Haitian Fang,Junnan Xu
To overcome the poor solubility and bioavailability of p -coumaric acid ( p -CA), an inclusion complex ( p -CA- β -CD) of p -CA and β -cyclodextrin ( β -CD) was prepared. FT-IR, XRD, DSC, and SEM were performed to characterize the p -CA- β -CD complex. Additionally, the antibacterial activity of p -CA- β -CD against Alicyclobacillus acidoterrestris and the underlying mechanisms were investigated. The results confirmed the successful fabrication of p -CA- β -CD and demonstrated a significant enhancement in p -CA solubility upon complexation with β -CD. p -CA- β -CD effectively inhibited A. acidoterrestris , with an MIC of 1.4 mg/mL, by damaging cell membrane, reducing intracellular pH and ATP, increasing ROS levels, and altering cell morphology. Moreover, 1MIC of p -CA- β -CD caused 2.89 and 2.18 log reductions of A. acidoterrestris during apple juice storage at 4 °C and 25 °C, respectively, without remarkable effect on physicochemical indexes and sensory quality. These findings suggested that p -CA- β -CD could potentially be an emerging and promising natural agent for controlling A. acidoterrestris in juice.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）