Due to the low adsorption capacity, poor adsorption selectivity and poor biocompatibility of bilirubin adsorption materials currently used, it is still a challenge to design an efficient bilirubin removal material for the treatment of hyperbilirubinemia. In this work, BSA-grafted (bovine serum albumin) porous amino polypyrrole was constructed by grafting bovine serum albumin onto porous amino polypyrrole composites. Comparing with porous amino polypyrrole, its removal rate was as high as 90% in 25 mg/L conjugated bilirubin solution, and it has significantly improved the selectivity of adsorption. In addition, it has good blood compatibility and biocompatibility. Superhydrophobic interaction, hydrogen bond and pore filling were the main reasons for bilirubin adsorption. BSA-grafted porous amino polypyrrole has potential application value in whole blood perfusion.
A colorimetric immunoassay is described for the pregnancy marker human chorionic gonadotropin (HCG). The assay is based on the use of MnO 2 nanorods acting as a peroxidase mimic. The nanorods were prepared by a hydrothermal method using EDTA as a template. They exhibit excellent peroxidase-like activity and stability. The nanorods were immobilized in a chitosan matrix in the wells of a BSA-modified microplate which then were further modified with streptavidin and biotinylated capture antibodies. The specific recognition between HCG and the antibodies in wells inhibit the peroxidase-like activity of the nanorods. Hence, the substrate 3,3′,5,5′-tetramethylbenzidine is less efficiently catalytically oxidized by H 2 O 2 to form a blue colored product. This results in a decrease in the absorbance at 652 nm. Response is linear in the 0.5 to 400 mIU·mL −1 HCG concentration range, and the detection limit is 0.36 mIU·mL −1 under optimized conditions. The method is highly specific, acceptably reproducible and stable. It was applied to the determination of HCG concentration in serum samples, and results were in good agreement with data obtained by the reference method.”
Schematic presentation of colorimetric immunoassay for human chorionic gonadotropin (HCG) by using MnO 2 nanorods with peroxidase-like activity immobilized in microplate wells. The specific recognition between HCG and the antibodies in wells inhibits the peroxidase-like activity of the nanorods. TMB: 3,3′,5,5′-tetramethylbenzidine.
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