SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN
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2023
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IF 0.7
Xiaoxiao LIU,Wenqing ZHANG,Tiantian FEI,Xiaosong HU,Zhongli HU,Surong JIN
In this work, deep eutectic solvent (DES) combined with ultrasonic-assisted extraction was proposed to extract free seleno-amino acids from Cardamine hupingshanensis. The contents of seleno-amino acids in the extract were determined by high-performance liquid chromatography with a fluorescence detector (HPLC-FLD). Response surface design was used to optimize the extraction conditions such as ultrasonic time, ultrasonic temperature, and liquid-solid ratio. The results showed that DES prepared by choline chloride and ethylene glycol had the highest extraction efficiency of seleno-amino acids. The main free seleno-amino acids in Cardamine hupingshanensis were selenocystine (SeCys2) and selenomethylcysteine (SeMeCys). The optimal extraction conditions were as follows: liquid-solid ratio of 50 mL·g–1; extraction temperature of 40 °C; and extraction time of 45 min. Under the optimal extraction conditions, the SeCys2 and SeMeCys contents were 356 μg·g–1 and 147 μg·g–1, respectively. This study shows that DES has great potential in extraction and separation.
研究领域:
植物化学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
STARCH-STARKE
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2021
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IF 2.741
Zimu Zhang,Xiufang Huang,Shichan Li,Kai Luo
Plant and fungal polysaccharides have been extensively studied for their anticoagulant, antioxidant, antitumor, immunomodulatory, and hypoglycemic activities. Furthermore, the inherent bioactivities of various polysaccharides can be improved by appropriate chemical modifications that can confer novel properties. Particularly, Cardamine hupingshanensis polysaccharides (CHPs) have been reported to exhibit curative action against diabetes in a mouse model. Therefore, in this study, CHPs are extracted by the hot water extraction method and modified by sulfation using the sulfur trioxide-pyridine method. Anion-exchange chromatography is performed to obtain pure, sulfated CHPs (SCHP-II). Nuclear magnetic resonance imaging and high-performance liquid chromatography are performed to determine the structure and composition of SCHP-II, respectively. The molecular weight of SCHP-II is determined to be 22.2 kDa. A n in vitro antioxidation activity assay performed using 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid showed that SCHP-II exhibited better free radical-scavenging ability than CHPs. Therefore, it is concluded that SCHP-II can be used as an antioxidant in food and medicinal products.
研究领域:
药理学
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生物化学
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食品科学
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药物化学
✓ 已验证
DOI
高影响因子
STARCH-STARKE
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2021
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IF 2.741
Meidong Li,Xiufang Huang,Qin Zhang,Yifeng Zhou,Kai Luo
Four new types of water-soluble polysaccharides are isolated from Cardamine hupingshanensis . Herein, this study examines C. hupingshanensis polysaccharide no. 2 (CHP-2) with a molecular weight of 21.75 kDa. Analysis revealed that CHP-2 is composed of d -glucuronic acid, d -galacturonic acid, glucose, galactose, and L-arabinose. It is speculated that the main connecting bond is →4)-α-D-Glc(p)-(1→), →t)-α-D-Glcp-(1→). Through perfusion experiments using streptozotocin-induced diabetic rats, oral CHP-2 solution is found to significantly reduce food intake, water intake, and fasting blood glucose levels, while increasing the weights of rats. In addition, CHP-2 significantly reduces the serum and liver malondialdehyde contents and increases the activity of the corresponding antioxidant enzymes. Moreover, the effect elicited by the CHP-2 solution is similar to that of phenformin hydrochloride solution. Hence, the results of this study suggest that CHP-2 exhibits blood sugar-lowering and anti-oxidation effects.
研究领域:
药理学
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生物化学
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糖尿病研究
✓ 已验证
DOI
高影响因子
STARCH-STARKE
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2021
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IF 2.741
Meidong Li,ZiMu Zhang,Ying Luo,Xiufang Huang,Kai Luo
Four new water-soluble polysaccharides has previously been isolated from Cardamine hupingshanensis . In this experiment, the structure of C. hupingshanensis polysaccharide NO. 3 (CHP-3) is analyzed and its in vitro anti-tumor activity is assessed. Through structural analysis, CHP-3 is found to have a molecular weight of 80.64 kDa, and be mainly composed of glucose, galactose, and L -arabinose; The backbone of CHP-3 is mainly composed of →5)-α-L-Araf-(1→ and →4)-α-D-Glc(p)-(1→), respectively. By studying its anti-tumor activity in vitro, it can be concluded that treatment with different concentrations of CHP-3 exerted diverse effects on A549 cells, with an obvious dose-dependent relationship. Moreover, after treatment with 12.8 mg mL −1 CHP-3, significant inhibitory effects are observed on the proliferation, migration, and invasion of A549 cells, indicating that it can be used as an anti-tumor agent for continued in-depth research.
研究领域:
药理学
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肿瘤学
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分子生物学
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化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）