JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Nuo Duan,Wenhui Gong,Shijia Wu,Zhouping Wang
Clenbuterol hydrochloride (CLB) is often abused as additive feed for livestock to decrease adipose tissue deposition and to increase growth rate. It raises a potential risk to human health through the consumption of animal product. In this study, aptamers with higher affinity and specificity were screened through 16 selection rounds based on the ssDNA library immobilized systematic evolution of ligands by exponential enrichment (SELEX) technique. After cloning and sequencing, five aptamer candidates were picked out for affinity and specificity assays based on a graphene oxide (GO) adsorption method. The results showed that the aptamer CLB-2 binds specifically against CLB with a dissociation constant, Kd, value of 76.61 ± 12.70 nM. In addition, an aptamer-based fluorescence bioassay was established for CLB analysis. The correlation between the CLB concentration and fluorescent signal was found to be linear within the range of 0.10 to 50 ng/mL with a limit of detection of 0.07 ng/mL. It has been further applied for the determination of CLB in pork samples, showing its great potential for sensitive analysis in food safety control.
研究领域:
食品安全
·
分子生物学
·
分析化学
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Liang Bai
Salbutamol, clenbuterol, ractopamine and albuterol have the effect of promoting protein synthesis and increasing muscle mass. Despite that these substances are banned or restricted in animal husbandry in many countries, some farmers still use them illegally to improve profits. Athletes can test positive for stimulants after ingesting food with these drug residues. In this study, the electrochemical behavior of salbutamol, clenbuterol, ractopamine and albuterol were investigated with carbon nanotubes modified glassy carbon electrodes. The linear detection range and detection limit of four stimulants were studied under optimal conditions.
研究领域:
分析化学
·
药物检测
·
食品安全
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Yun Shu,Shiyuan Liu,Juewen Liu
In this work, a library-immobilization method was used to select DNA aptamers for ractopamine (RAC) and clenbuterol (CLB), two common “lean meat agents”. Two RAC-binding aptamers, RAC-1 and RAC-5, exhibit dissociation constants (Kd) of 6.8 and 2.9 μM, respectively, based on isothermal titration calorimetry (ITC). Similar values were also obtained using a thioflavin T (ThT) fluorescence assay. A CLB aptamer named CLB-1 exhibited a Kd of 561 nM by the ThT assay and 885 nM by ITC. The RAC-5 aptamer showed a modest response to CLB, whereas CLB-1 exhibited strong specificity to CLB. Comparisons were made with previously published aptamers, and the aptamers reported here were shorter, had well-defined secondary structures, and were validated by rigorous binding assays. Furthermore, the ThT fluorescence responses of RAC-5 and CLB-1 were used to develop a label-free fluorescent sensor array to distinguish RAC and CLB from each other and from other structurally related molecules. These individual sensors had limits of detection of 1.2 μM for RAC and 0.16 μM for CLB. Finally, the label-free aptamer sensor was applied to spiked cat food and pork samples. These results demonstrate the potential of these aptamers for practical applications in food additive monitoring and safety assessment.
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 12
·1-10
·
IF 5.705
Ge Yu,Camarada María Belén,Xu Lanjiao,Qu Mingren,Liang Huan,Zhao Erlong,Li Mingfang,Wen Yangping
A nanocomposite was prepared from graphene-like two-dimensional black phosphorene (BP, an allotrope of phosphorus) and nafion (Nf) treated with isopropanol (IP). A glassy carbon electrode (GCE) modified with this nanocomposite was found to be a viable sensor for voltammetric determination of clenbuterol (CLB). Unlike previously reported pure BP, the BP nanocomposite was stable towards water and oxygen. Its morphology, structure, electrochemically active surface area and electrochemical stability were investigated. The BP-Nf (IP) modified GCE displayed good electrochemical stability and electrocatalytic capacity with a low working potential of 0.94 V (vs. SCE), excellent peak current response for CLB in a linear concentration range of 0.06–24 μM with a detection limit of 3.7 nM (3σ/m) and a sensitivity of 0.14 μA·μM −1 ·cm −2 under optimal conditions. A sensing mechanism for the electro-oxidation of CLB was suggested and verified by density functional theory calculations under imitation of aqueous solution conditions. The sensor was successfully applied to the determination of CLB in bovine meat and bovine serum samples.
Highly-stable black phosphorene (BP) nanocomposite based on Nafion (Nf) was used to modify a glassy carbon electrode (GCE). It is shonw to be a viable electrochemical platform for sensitive voltammetric determination of trace clenbuterol (CLB) in bovine beef and bovine serum.
研究领域:
分析化学
·
材料科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2019
·Vol. 189
·期 3
·960-971
·
IF 2.14
Wang Mengjun,Zhu Mengde,Wang Yu,Fan Zichun,Wu Shining,Zhang Xiaobo,Tong Zhiwei
In this paper, we synthesized HNbMoO 6 /C composite through the calcination of octylamine-intercalated HNbMoO 6 precursor. The resulting HNbMoO 6 /C composite showed some new phases of MoO 2 , MoO 3 , NbO 2 , Nb 2 O 5 , and carbon, which was fully confirmed via powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), and X-ray photoelectron spectroscopy (XPS) technologies. Besides, the HNbMoO 6 /C hybrid was coated on glass carbon electrode to construct an electrochemical sensor for sensitive determination of clenbuterol. The electrochemical behaviors of clenbuterol on the prepared electrode were tested by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) analysis. The results showed that the intercalated carbon can act as active sites to accelerate electron transfer. In addition, more exposed surface areas of the HNbMoO 6 /C composite will facilitate the electrolyte to permeate. The oxidation peak current of clenbuterol was linearly related to its concentration in the range of 1.04 × 10 −5 to 7.51 × 10 −4 mol L −1 , and the determination limit was calculated to be 3.03 × 10 −6 mol L −1 (S/N = 3). This sensor exhibits excellent stability, reproducibility, specificity, and good recoveries when applied to monitor clenbuterol in real samples.
研究领域:
分析化学
·
纳米材料科学
·
环境监测
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yanxue Xu,Xilan Gao,Jie Cheng,Hong Chen,Tao Tao,Maoping Liu,Dan Xiao
As the illegal use of clenbuterol (CLB) in animal husbandry introduced various diseases, the accurate and convenient methods for CLB detection is imperative to be developed. Because of the electrochemical activity of CLB, electrochemical strategies have been proved to be capable to achieve its detection. To improve the microenvironment of the electrode surface to enhance the response, the sensing materials with superior performance still need to be developed. In this work, a kind of environmentally friendly porous hard carbon (PHC) derived from the waste of coffee bean has been prepared to be utilized for the conveniently detection of CLB. Characterizations reveal the porous structure and lattice disorderliness of PHC. PHC demonstrates highly active surface, good conductivity and excellent chemical stability during the electrochemical tests. CLB produces obvious anodic peak on PHC modified glassy carbon electrode (PHC/GCE). The KOH/H 2 O 2 activated PHC/GCE (b-PHC/GCE) achieves a prominently enhanced peak current, which is about 2.5-fold of that recorded by PHC/GCE. The linear dynamic obtained by SWV and DPV is 50 nM-7.0 μM, the LODs (3σ/s) are 10.2 nM and 8.8 nM, respectively. CLB in meat samples cannot be found, the recoveries are in the range of 88.8% ~ 98.7% and the RSD is 5.09%. The accuracy of the results can be verified by LC-MS/MS. The PHC demonstrates its advantages of low-cost, environmentally friendly and superior sensing performance. The activation of the sensor is facile and fast, and the obtained b-PHC/GCE is reliable in practical use, which achieves the rapid monitoring of the forbidden of harmful additives.
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2011
·
IF 4.311
Guichi Zhu,Yongjun Hu,Jiao Gao,Liang Zhong
In this report, we present a novel approach to detect clenbuterol based on competitive surface-enhanced Raman scattering (SERS) immunoassay. Herein, a SERS nanoprobe that relies on gold nanoparticle (GNP) is labeled by 4,4′-dipyridyl (DP) and clenbuterol antibody, respectively. The detection of clenbuterol is carried out by competitive binding between free clenbuterol and clenbuterol-BSA fastened on the substrate with their antibody labeled on SERS nanoprobes. The present method allows us to detect clenbuterol over a much wider concentration range (0.1–100 pg mL −1 ) with a lower limit of detection (ca. 0.1 pg mL −1 ) than the conventional methods. Furthermore, by the use of this new competitive SERS immunoassay, the clenbuterol-BSA (antigen) is chosen to fasten on the substrate instead of the clenbuterol antibody, which could reduce the cost of the assay. Results demonstrate that the proposed method has the wide potential applications in food safety and agonist control.
研究领域:
食品安全
·
分析化学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Yingnan Liu,Yaqing Xiao,Min Yu,Yuanyuan Cao,Fan Li,Pei Jia,Du Guo,Xinyu Sun,Li Wang
In view of the potential harm caused by illegal feeding of clenbuterol (CLB) in the livestock industry, herein, a novel ratiometric fluorescent probe based on graphene quantum dots (GQDs)@[Ru(bpy)3]2+ was elaborately constructed for CLB detection. In this probe, GQDs acted as response signals, and their fluorescence was remarkably quenched by CLB through the diazotization-coupling reaction. As for [Ru(bpy)3]2+ as a reference signal, its fluorescence was hardly affected. The intensity ratio of two fluorophores showed good linearity with CLB concentration in the range of 0.05–40 μM, accompanied by visualization of fluorescence variation from yellow to red. The detection limit was as low as 0.029 μM. Particularly, the probe was successfully used to detect CLB in pork and beef samples with satisfactory recoveries. To our knowledge, this is the first report on a ratiometric fluorescent probe for the detection of CLB, which possesses broad application prospects in food safety risk monitoring.
研究领域:
分析化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Xin-Jie Song,Fei Ye,Yao Zhang,Juan Sun,Xuping Shentu,Xiaoping Yu,Wei Li,Yuan-Feng Wu
In this study, a fluorescence detection method combining aptamer-modified up-conversion nanoparticles (UCNPs) and magnetic nanoparticles (MNPs) was developed for detection of Clenbuterol (CLB). The aptamer-modified magnetic NPs captured CLB, which reacted with the aptamer-modified UCNPs and generated a sandwich complex. The aptamer-modified UCNPs acted as a fluorescence source. The MNP-CLB-UCNP complex was retrieved from the solution using an magnetic field, and the fluorescence intensity was detected by fluorescence spectrophotometry with excitation and emission spectra at 980 nm and in the 400–800 nm region, respectively. The results showed that the fluorescence intensity gradually increased with increasing concentrations of CLB with a good specify. The method was highly sensitive for the quantification of CLB, with a limit of detection of 0.304 ng mL −1 . The recovery rate of CLB from pork samples ranged from 84 % to 94.87 %. This fluorescence method enables the sensitive, precise, and accurate quantification of CLB residues in pork samples.
研究领域:
分析化学
·
食品安全
·
生物医学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Huan Jing,Huiying Ouyang,Weifeng Li,Yumei Long
A new strategy was demonstrated for the electrochemical detection of clenbuterol using boron carbon oxynitride (BCNO) nanosheets as a sensing medium. BCNO was prepared via a molten salt process and structurally characterized. The experimental results, including XRD, FTIR, XPS, TEM and AFM, suggested that the as-prepared sample is crystalline BCNO with a nanosheet-like structure. After modification on a glassy carbon electrode (GCE), BCNO can significantly improve the electron transport capacity and provide more electrochemically active sites. Interestingly, BCNO-modified GCE (BCNO/GCE) possesses good capability for electrocatalytic oxidation of clenbuterol and thus produces noticeable electrochemical signals. By using the differential pulse stripping voltammetry (DPSV) method, the BCNO/GCE exhibited superior analytical performance in the detection of clenbuterol. The sensor has a low detection limit of 17 nM, and its linear concentration ranges are 0.03–2.0 and 2.0–16.0 μM, respectively. In addition, the clenbuterol sensor displayed good anti-interference ability, high reproducibility and adequate stability. Finally, the proposed method was applied to measure clenbuterol in pig serum and drug tablets, and satisfactory recoveries were achieved. The research results suggested that BCNO nanostructures should be promising candidates for the fabrication of electrochemical sensors.
研究领域:
材料科学
·
电化学
·
分析化学
·
纳米技术
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产品关联
DOI
高影响因子
MEDICINAL CHEMISTRY RESEARCH
·
2017
·Vol. 27
·期 3
·944-953
·
IF 1.277
Wang Zechun,Han Xinxin,Wang Ning,Wang Ruiyong,Chang Junbiao
Clenbuterol (CLB) is a notorious “lean meat essence”, which has harmful effects on human health. In this study, the binding of CLB to two proteins, human serum albumin (HSA) and the human fat mass and obesity associated protein (FTO), have been investigated by spectroscopic and molecular modeling methods. Fluorescence data revealed that CLB could bind to HSA (or FTO) to form a complex. Thermodynamic analysis and molecular modeling indicated that the binding process was a spontaneous molecular interaction procedure, in which hydrophobic interaction played a major role. Results indicated that CLB could lead to some microenvironmental changes of both proteins.
研究领域:
生物化学
·
药物学
·
分子生物学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Lu Yang,Tong Zhai,Jing Wang,Hong Zheng,Jing-Min Liu,Shuo Wang
To achieve high throughput detection of clenbuterol while reducing matrix interference, we developed a simple and integrated nano-inspection platform. Herein, based on the simplicity and convenience of paper chip, a multi-layer paper chip-chemiluminescence (CL) sensor was designed in the form of “48-well plate” type. The all-in-one paper chip-CL sensor has integrated the covalent organic frameworks (COFs) layer for sample matrix purification, high affinity nanobody layer for specific capture of target, and CL rapid response layer for the final determination. The signal readout was realized by the inhibitory effect of clenbuterol on the K 3 [Fe(CN) 6 ] CL system. Detection of clenbuterol in pork meat was verified by measuring the CL intensity and satisfactory recoveries (85.9 %–97.3 %) were achieved. 48 samples could be simultaneously detected on one-chip in one-test, favoring the high-throughput detection. Especially, the work explored a new pathway for design of portable analytical instruments to achieve immediate on-site detection of hazards.
研究领域:
分析化学
·
食品安全检测
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）