BIOSENSORS & BIOELECTRONICS
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2018
·
IF 8.173
Han Zhang,Cong Zhang,Di Liu,Fumei Zuo,Shihong Chen,Ruo Yuan,Wenju Xu
A novel ratiometric electrochemiluminescent (ECL) biosensor was designed for the detection of concanavalin A (Con A) based on two ECL emitters competing for the dissolved oxygen (O 2 ). In this strategy, CdTe quantum dots (QDs) were used as the cathodic emitter and N-(aminobutyl)-N-(ethylisoluminol) (ABEI) was used as the anodic emitter. With the presence of dissolved O 2 utilized as co-reactant, CdTe QDs showed an ECL emission at − 1.7 V, and ABEI showed an emission at + 0.6 V. Phenoxy-derivatized dextran (Dexp), as a recognition element, was immobilized by graphene oxide functionalized CdTe QDs (G-CdTe QDs) to recognize Con A, and further combine with Dexp, gold and platinum nanoparticles decorated ABEI (Dexp-Au-Pt-ABEI) to form a sandwich structure. With the increasing concentration of analyte Con A, the ECL signal of cathodic CdTe QDs decreased and the anodic response of ABEI increased, thus achieving a ratiometry detection of Con A with a wide linear range from 1.0 × 10 −4 ng/mL to 10 ng/mL. The detection limit was low to 3.0 × 10 −5 ng/mL. This proposed ratiometric ECL biosensor not only conquered the false errors caused by external interferences, but excluded the disability caused by exogenous co-reactant via the application of common dissolved O 2 . It also exhibited an excellent stability, selectivity and reproducibility.
研究领域:
生物传感器技术
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分析化学
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产品关联
DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 28
·9714-9720
·
IF 4
Sirui Liu,Yuxin Shi,Lingling Xu,Weican Zhan,Meixi Chen,Xiaoyue Pan,Yuqing Yao,Jiajie Cai,Mingyi Zhang,Xinzhi Ma
Designing an excellent OER catalyst in an alkaline environment is severe yet essential for industrial H2 application under the electrochemical technique. This study has achieved multiple modifications on CoN nanowires, the classic OER catalyst, via a facile room-temperature NaBH4 spontaneous hydrolysis. This facile process simultaneously generates oxygen vacancies and robust BN species. It wraps hydrophilic BOx motifs on the OER response CoN nanowires, producing OER active Co–N–B species, increasing active numbers and guaranteeing structural stability. It suggests that a low NaBH4 concentration (0.1 mol L−1) treatment endows CoNNWAs/CC with excellent OER performance and robust structure, which can drive a current density of 50 mA cm−2 with only 325 mV overpotentials with more than 24 hours’ durability. Even, the catalyst can drive 1000 mA cm−2 around 480 mV overpotential. This study allows a novel strategy for designing high-performance OER catalysts.
研究领域:
材料科学
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电化学
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催化反应
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FUEL
·
2022
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IF 8.035
Tingzhen Chen,Chengwu Yang,Saravanan Rajendran,Yongpeng Lei,Xinyu Zhang,Jiaqian Qin
The photocatalytic water splitting to produce hydrogen is of great significance to solve the energy crisis, so the development and research of the visible-light-driven energy conversion have the priority in the progress of photocatalytic technology. Herein, we synthesized CdS with hollow sphere (CdS-HS) structure and combined it with CoN to form CoN/CdS-HS photocatalytic system. The particular hollow sphere structure and cocatalyst CoN in the CoN/CdS-HS system increase the utilization of visible light and promote the separation and transfer of photo-generated carriers for photocatalysts, which greatly improves photocatalytic performance of materials. The time‐resolved photoluminescence spectra shows that the carrier lifetime of 15%CoN/CdS-HS is 7.52 ns, which is much larger than that of CdS (4.26 ns). In addition, the hydrogen output of the optimized 15%CoN/CdS-HS is 4.57 times superior to that of pristine CdS-HS under the same conditions. The photocatalytic hydrogen evolution rate of 15%CoN/CdS-HS reaches 58.80 mmol/g . h under visible light with an apparent quantum efficiency (AQE) of 23.53% at 405 nm monochromatic light. After 25 h of photocatalytic reaction, the 15%CoN/CdS-HS can still maintain excellent overall stability. This work provides an idea for the design and development of efficient visible-light-driven photocatalysts in the future.
研究领域:
材料科学
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能源科学
✓ 已验证
DOI
高影响因子
ACS Nano
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2017
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IF 13.942
Jie Yin,Yuxuan Li,Fan Lv,Qiaohui Fan,Yong-Qing Zhao,Qiaolan Zhang,Wei Wang,Fangyi Cheng,Pinxian Xi,Shaojun Guo
The development of highly efficient bifunctional catalysts for oxygen evolution reaction (OER) and oxygen reduction reaction (ORR) is crucial for improving the efficiency of the Zn–air battery. Herein, we report porous NiO/CoN interface nanowire arrays (PINWs) with both oxygen vacancies and a strongly interconnected nanointerface between NiO and CoN domains for promoting the electrocatalytic performance and stability for OER and ORR. Extended X-ray absorption fine structure spectroscopy, electron spin resonance, and high-resolution transmission electron microscopy investigations demonstrate that the decrease of the coordination number for cobalt, the enhanced oxygen vacancies on the NiO/CoN nanointerface, and strongly coupled nanointerface between NiO and CoN domains are responsible for the good bifunctional electrocatalytic performance of NiO/CoN PINWs. The primary Zn–air batteries, using NiO/CoN PINWs as an air–cathode, display an open-circuit potential of 1.46 V, a high power density of 79.6 mW cm–2, and an energy density of 945 Wh kg–1. The three-series solid batteries fabricated by NiO/CoN PINWs can support a timer to work for more than 12 h. This work demonstrates the importance of interface coupling and oxygen vacancies in the development of high-performance Zn–air batteries.
研究领域:
材料科学
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 24
·2101324
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IF 18.808
Daying Guo,Zhihao Zeng,Zhixin Wan,Yan Li,Bin Xi,Chengxin Wang
Reported herein is an active and durable CoN-containing oxygen evolution reaction (OER) electrocatalyst which efficiently functions in a neutral medium (pH ≈7). The composite material (N, S)-RGO@CoN is synthesized by delicate atomic layer deposition (ALD) of CoN on a nitrogen and sulfur (N, S) co-doped reduced graphene oxide (RGO) substrate. Representative results of the comprehensive study are: 1) The flower-like sphere RGO substrate prepared by spray drying method features rich physical and chemical properties, which are beneficial for rapid mass/charge transfer to improve the intrinsic OER process; 2) the optimal ALD material for OER tests is afforded by tuning spray conditions and ALD parameters. Versatile structural and compositional characterizations confirm uniform growth and strong chemical coupling of nanostructured CoN on (N, S)-RGO matrix; 3) the material is electrocatalytically active and durable in a neutral electrolyte, recording an OER overpotential of 220 mV at a current density of 10 mA cm −2 and stability of 20 h continuous catalysis at 20 mA cm −2 with nearly 100% Faradic efficiency; 4) Upon the experimental studies and density functional theory calculations, the eventual mechanism of remarkable OER activity conforms to the structural fate of ALD CoN electronic coupling to the carbon substrate.
研究领域:
材料科学
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电化学
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催化科学
✓ 已验证
DOI
高影响因子
ELECTROCHIMICA ACTA
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2018
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IF 5.116
Fumei Zuo,Cong Zhang,Han Zhang,Xingrong Tan,Shihong Chen,Ruo Yuan
A solid-state electrochemiluminescence (ECL) biosensor for the detection of concanavalin A (Con A) was constructed using graphene quantum dots (GQDs) as luminophore, which were loaded on polyamidoamine (PAMAM) functionalized CeO 2 @Ag NPs by the interaction between the NH 2 of PAMAM and the COOH of GQDs. The electrodepositing gold layers were used as matrix to immobilize glucose oxidase (GOx). GOx served as recognition elements to further capture Con A by the specific interaction between carbohydrate and Con A. With the increase of the concentration of Con A, the amount of GOx-CeO 2 @Ag-GQDs incubated onto the electrode was increased and a higher ECL signal was obtained. The constructed biosensor exhibited a sensitive response towards Con A and a low detection limit of 0.16 pg/mL was obtained. Such a construction strategy provides a new approach for applying GQDs to establish a solid-state ECL biosensor.
研究领域:
生物传感
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Inorganic Chemistry Frontiers
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2018
·Vol. 5
·期 11
·2906-2913
·
IF 5.106
Jiali Li,Xianggui Kong,Meihong Jiang,Xiaodong Lei
Exploring nonprecious, efficient and stable electrocatalysts for overall water splitting is of great importance, but it is also challenging. In this study, N-doped carbon@ CoN/Cu3N/copper foam (namely, NC@CoN/Cu3N/CF) nanoarrays with a hollow tubular structure have been successfully synthesized through a facile method. The as-produced NC@CoN/Cu3N/CF with a 3D hierarchical structure was highly beneficial for electrocatalysis; it displayed improved electrical conductivity, abundant exposed active catalytic sites, numerous “highways” for charge transport and synergistic effect of each component. The enhanced characteristics resulted in largely improved kinetics and activity for the oxygen evolution reaction (OER) and hydrogen evolution reaction (HER). The NC@CoN/Cu3N/CF electrode exhibited low overpotentials of 303 mV to reach a current density of 50 mA cm−2 for OER and 134 mV to reach a current density of 10 mA cm−2 for HER in an alkaline solution. Furthermore, as an electrocatalyst for overall water splitting, NC@CoN/Cu3N/CF served as both the anode and cathode, delivering a current density of 10 mA cm−2 at a quite low cell voltage of 1.62 V, and it displayed excellent long-term stability (15 h at 20 mA cm−2). This study provides a rational strategy and approach to fabricate transition metal nitrides with 3D hierarchical structures as efficient electrocatalysts in renewable energy applications.
研究领域:
材料科学
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电化学
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可再生能源
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催化
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Chenze Lu,Gongyan Wang,Xiaoxiao Zhang,Zhihui Fang,Xinyuan Wang,Fan Tang,Kang Ning,Min Xu,Jing Wang,Han Jiang,Ran Tao,Pei Xu
Intake of Concanavalin A (Con A), a hazardous lectin protein commonly discovered in legume vegetables, could cause various systemic symptoms including rash, nausea, vomiting, diarrhea, etc. Herein, we present a novel label-free and cost-effective electrochemical Con A aptasensor. [Fe(CN) 6 ] 3−/4- ions in testing buffer not only generate redox peaks during electrochemical scan, but also serve as electron acceptor/donor to enhance electron transfer. Anti-Con A aptamer is immobilized on surface of screen printed electrode to capture Con A molecules and form aptamer-protein complex, which hinders electron transfer near electrode surface and causes a decrease in peak current value. The constructed aptasensor displays a low detection limit of 17.88 ng/mL (around 171.92 pM), as well as good specificity against other legume lectins. The practicality of electrochemical sensors was evaluated using real-world samples, demonstrating their ability to rapid detect Con A in crude protein extracts from jack bean seeds. This work provides novel tools for rapid evaluation of Con A content in massive samples, which is required in food safety inspection and safety breeding of legume vegetables.
研究领域:
生物传感技术
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食品安全
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2015
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IF 5.636
Liping Dong,Shun Feng,Shanshan Li,Peipei Song,Jide Wang
In this work, a soft and nondestructive approach was developed to prepare concanavalin A-chelating magnetic nanoparticles (Con A-MNPs) for selective enrichment of glycoproteins. Ethylenediamine tetraacetic acid-modified-MNPs (EDTA-MNPs) were prepared by a one-pot chemical coprecipitation method first, and then, Cu(II) cations were used as bridge groups to immobilize Con A on EDTA-MNPs. The as-prepared absorbents with a mean diameter of 15 nm showed a strong magnetic response to an externally applied magnetic field. The results of thermogravimetric analysis showed the content of immobilized Con A was up to 28 wt %. For glycoprotein ovalbumin, the maximum capacity and equilibrium constant were 72.41 mg/g and 0.6035 L/mg, respectively. The as-prepared nanocomposites exhibited a remarkable selectivity for glycoproteins and can enrich glycoproteins specifically from a mixture of glycoprotein and nonglycoprotein even at a molar ratio of 1:600. It was also successfully applied for the enrichment of glycoproteins from real egg white samples. We expect that our finding will serve as a helpful template for others to design new adsorbents for enriching glycoproteins.
研究领域:
生物化学
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纳米技术
·
蛋白质组学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2015
·
IF 3.545
Shenguang Ge,Lina Zhang,Yan Zhang,Haiyun Liu,Jiadong Huang,Mei Yan,Jinghua Yu
A low-cost, simple, portable and sensitive paper-based electrochemical sensor was established for the detection of K-562 cell in point-of-care testing. The hybrid material of 3D Au nanoparticles/graphene (3D Au NPs/GN) with high specific surface area and ionic liquid (IL) with widened electrochemical windows improved the good biocompatibility and high conductivity was modified on paper working electrode (PWE) by the classic assembly method and then employed as the sensing surface. IL could not only enhance the electron transfer ability but also provide sensing recognition interface for the conjugation of Con A with cells, with the cell capture efficiency and the sensitivity of biosensor strengthened simultaneously. Concanavalin A (Con A) immobilization matrix was used to capture cells. As proof-of-concept, the paper-based electrochemical sensor for the detection of K-562 cells was developed. With such sandwich-type assay format, K-562 cells as model cells were captured on the surface of Con A/IL/3D AuNPs@GN/PWE. Con A-labeled dendritic PdAg NPs were captured on the surface of K-562 cells. Such dendritic PdAg NPs worked as catalysts promoting the oxidation of thionine (TH) by H 2 O 2 which was released from K-562 cells via the stimulation of phorbol 12-myristate-13-acetate (PMA). Therefore, the current signal response was dependent on the amount of PdAg NPs and the concentration of H 2 O 2 , the latter of which corresponded with the releasing amount from cells. So, the detection method of K-562 cell was also developed. Under optimized experimental conditions, 1.5×10 −14 mol of H 2 O 2 releasing from each cell was calculated. The linear range and the detection limit for K-562 cells were determined to be 1.0×10 3 –5.0×10 6 cells/mL and 200 cells/mL, respectively. Such as-prepared sensor showed excellent analytical performance with good fabrication reproducibility, acceptable precision and satisfied accuracy, providing a novel protocol in point-of-care testing of cells.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2024
·
IF 3.2
Yan Lu,Weilin Weng,Yuanhang Lu,Xinxin Pang,Naixuan Hu,Banghua Peng
Hydrogen is increasingly recognized as a critical energy source for the future. Transition metal nitrides hydrogen catalysts have found to narrow the d-band contraction effectively and alter the filling state of the metal, thereby modulating the metal-hydrogen bonding energy and enhancing both electrical conductivity and catalytic activity. In this study, V-doped cobalt nitrides (V–Co 2 N/CoN) with two distinct crystalline structures were successfully synthesized through the nitriding of V-doped cobalt bicarbonate (V–CoCH) as a precursor. The morphology and structure of the resulting materials were carefully optimized by adjusting the V doping amount and nitriding temperature. For hydrogen evolution reaction (HER) and oxygen evolution reaction (OER), the final product V–Co 2 N/CoN-1-400 only needs 47 mV and 230 mV overpotential to reach 10 mA cm −2 , and works at 1.69 V to drive the overall water splitting for 10 mA cm −2 , showing good stability performance without fluctuation for about 10 h. This work implies that V-doped cobalt nitrides can lead to enhanced water splitting catalytic activity. Our findings may illustrate the significance of V doping for improving electrocatalytic performance, and demonstrate the potential of CoV bimetallic nitrides as effective electrocatalysts , offering valuable insights for the further development of non-precious metal nitride-based catalysts.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2011
·
IF 2.502
Ruixue Yin,Zi Tong,Dongzhi Yang,Jun Nie
Glucose and pH dual-responsive microhydrogels based on concanavalin A (Con A) were prepared and used for insulin delivery. The combination of the specific saccharide-binding affinity of Con A and the cationic groups of N-(2-(dimethylamino) ethyl)-methacrylamide (DMAEMA) led to dual-responsive systems. SEM, fluorescence microscopy and particle size analysis showed that the obtained microhydrogels had a dense surface morphology and an average size of 38 μm. The in vitro insulin release study revealed that the microhydrogels could quickly respond to the changes of glucose concentrations in the medium and small change in pH value of the environment. The kinetics of insulin release was analyzed by using empirical equation and the apparent diffusion coefficient was calculated according to a solution of Fick's second law. The released insulin was proved to remain active. The result suggested that this microhydrogel might find potential applications for self-regulated insulin delivery, actuators and separation systems with sensitivity to glucose.
研究领域:
生物材料学
·
药物递送系统
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）