JOURNAL OF CHEMICAL THERMODYNAMICS
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2016
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IF 2.196
Dandan Han,Yumin Liu,Xiaona Li,Teng Zhang,Zhenfang Li,Shichao Du,Mingxia Guo,Shijie Xu,Bo Yu,Baohong Hou,Junbo Gong
The solubility of calcium d -pantothenate in (water + methanol) mixtures and the effect of β -alanine on the solubility of calcium d -pantothenate in a constant mole ratio of methanol and water was measured from 263.15 K to 303.15 K by the HPLC analysis method ( P = 0.1 MPa). The solubility of calcium d -pantothenate increased with increasing temperature in all the mixtures at constant composition. Besides, it also increased with mole fraction of water in (water + methanol) mixtures at a constant temperature. Additionally, the solubility of calcium d -pantothenate was enhanced by β -alanine at a given temperature. The experimental solubility in (water + methanol) mixtures was correlated by the modified Apelblat equation, the ternary NRTL equation and Solubility-Polarity Model. The Apelblat equation, the quaternary NRTL equation and the Cohn equation were adopted to correlate the experimental solubility in the (water + methanol + β -alanine) mixtures. Worthwhile, the quaternary NRTL equation was first applied to correlating the quaternary solid-liquid equilibrium. It turned out that all the selected models give good performance on solubility prediction.
研究领域:
药物化学
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物理化学
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2018
·Vol. 102
·期 14
·6039-6046
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IF 3.34
Tigu Fitsum,Zhang Junli,Liu Guoxia,Cai Zhen,Li Yin
d -Pantothenic acid (vitamin B 5 ) has wide applications in the feed, food, chemical, and pharmaceutical industries. Its biological production routes which employ pantothenate synthetase (PS) as the key enzyme are attractive since they avoid the tedious and time-consuming optical resolution process. However, little data is available on the activity and kinetics of this enzyme, hampering the rational selection of an efficient enzyme for the biological production of d -pantothenic acid. In this study, six phylogenetically distant PS-encoding genes, from Escherichia coli , Corynebacterium glutamicum , Bacillus subtilis , Bacillus thuringiensis , Bacillus cereus , and Enterobacter cloacae , were expressed in E. coli . The PS from C. glutamicum exhibited a specific activity of 205.1 U/mg and a turnover number of 127.6 s -1 , which to our best knowledge are the highest values ever reported. The addition of substrates ( d -pantoic acid and β-alanine) to the E. coli strain harboring this enzyme during the early log phase of fermentation resulted in the production of 97.1 g/L of d -pantothenic acid within 32 h, corresponding to a conversion yield of 99.1% and a productivity of 3.0 g/L/h. To the best of our knowledge, this is the highest productivity reported to date.
研究领域:
生物化学
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微生物学
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工业生物技术
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PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Yunhe Zhang,Yun Huang,Changjian Zhang,Yang Gao,Yanzhou Wang,Caixia Li,Yiheng Wang,Xing Li,Mingshan Wang,Yuanhua Lin
Aqueous zinc-ion batteries (AZIBs) have gained increasing attention for grid energy storage systems. However, ensuring the long-term reversible operation of the zinc anode remains a challenge due to dendrite growth and adverse side reactions during the charge and discharge cycles. This study investigates the use of d-pantothenic acid (D-PA) as an additive in 2 M ZnSO4 aqueous electrolyte to enhance the cycling stability of the zinc anode in AZIBs. Experimental results and theoretical calculations demonstrate that D-PA reshapes the solvation structure of Zn2+ by partially replacing coordinated water molecules, ensuring the stability of Zn2+ transport. Furthermore, D-PA adsorbs on active sites of the zinc anode, increasing the surface overpotential (|ηs|), reducing the nucleation energy barrier, and decreasing the critical nucleus size (rcrit), thus ensuring uniform zinc deposition. This dual role of modifying the Zn2+ solvation shell and regulating Zn2+ nucleation effectively mitigates dendrite growth and suppresses side reactions, resulting in excellent stability of the zinc anode. Consequently, Zn||Zn symmetrical cells with the D-PA additive maintain stable operation for over 2000 h at 1.0 mA cm–2 and 1.0 mA h cm–2, and nearly 4000 h at 4.0 mA cm–2 and 4.0 mA h cm–2. Additionally, Zn||Cu asymmetric cells exhibit cycling stability over 300 cycles at 0.5 mA cm–2 and 0.5 mA h cm–2, with an average Coulombic efficiency of 99.29%. Moreover, Zn||V2O5 full cells containing the D-PA additive exhibit stable cycling performance over 1000 cycles at a current density of 1 A g–1, maintaining a high capacity retention. Specifically, the initial capacity of the full cell is around 161.17 mA h g–1, with approximately 62.7% capacity retention after 1000 cycles.
研究领域:
材料科学
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电化学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Jiaxuan Guo,Xixi Sun,Yujie Yuan,Qitong Chen,Zutian Ou,Zixin Deng,Tian Ma,Tiangang Liu
Vitamin B5, also called d-pantothenic acid, is an essential vitamin in the human body and is widely used in pharmaceuticals, nutritional supplements, food, and cosmetics. However, few studies have investigated the microbial production of d-pantothenic acid, especially in Saccharomyces cerevisiae. By employing a systematic optimization strategy, we screened seven key genes in d-pantothenic acid biosynthesis from diverse species, including bacteria, yeast, fungi, algae, plants, animals, etc., and constructed an efficient heterologous d-pantothenic acid pathway in S. cerevisiae. By adjusting the copy number of the pathway modules, knocking out the endogenous bypass gene, balancing NADPH utilization, and regulating the GAL inducible system, a high-yield d-pantothenic acid-producing strain, DPA171, which can regulate gene expression using glucose, was constructed. By optimizing fed-batch fermentation, DPA171 produced 4.1 g/L d-pantothenic acid, which is the highest titer in S. cerevisiae to date. This study provides guidance for the development of vitamin B5 microbial cell factories.
研究领域:
生物技术
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代谢工程
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微生物生物技术
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PubMed
高影响因子
Food Bioscience
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2021
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IF 4.24
Bo Zhang,Li Chen,Jie-Yi Jin,Na Zhong,Xue Cai,Shu-Ping Zou,Hai-Yan Zhou,Zhi-Qiang Liu,Yu-Guo Zheng
: As the key precursor of coenzyme A synthesis in vivo , D-pantothenic acid plays an important role in maintaining biological function, which is widely applied in the food , pharmaceutical, and cosmetic industries. At present, it was mainly produced by chemical methods, with serious environmental pollution, therefore, it is urgent to develop an economical, practical, and environmentally friendly biological fermentation method based on microbial chassis cells. Herein, based on our previously developed D-pantothenic acid producing strains, we obtained higher yielding D-pantothenic acid producing strains by systematic metabolic engineering . First, according to the results of CRISPRi experiments, lpd were overexpressed, disrupted the non-PTS (non-phosphoenolpyruvate-pyruvate transport system) as well as enhanced the PTS (phosphoenolpyruvate-pyruvate transport system) allowing precursor accumulation. Subsequently, based on the analysis of metabolic intermediates , the pyruvate bypass pathway was blocked as well as the (R)-pantothenic acid pathway was further enhanced, increasing the titer of D-pantothenic acid to 4.60 g/L. Finally, the organic acid and branched-chain amino acid synthesis pathways were blocked, and in order to accelerate the synthesis of acetolactate and the output of D-pantothenic acid, heterologous acetolactate synthase and pantothenate transfer protein were introduced, which significantly increased the yield of D-pantothenic acid, reach to 6.33 g/L. The final strain in a 5 L bioreactor produced 32.32 g/L D-pantothenic acid with a productivity of 2.33 g/L/h and the high-yielding strain provides an effective platform for biological fermentation to produce D-pantothenic acid.
研究领域:
生物技术
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代谢工程
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微生物学
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发酵技术
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Journal of Materials Chemistry A
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2023
·Vol. 11
·期 26
·14311-14319
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IF 11.9
Lantao Liu,Ziyu Sun,Yaping Lu,Jiapeng Zhang,Yiming Li,Gaixia Zhang,Xiaohong Chen,Sasha Omanovic,Shuhui Sun,Huaihe Song
Due to the environmental friendliness and high safety of aqueous Zn-ion hybrid capacitors (ZIHCs), the exploration of high-performance porous carbon electrodes for ZIHCs has recently attracted significant attention. However, most of the reports focus on the preparation method and modification of porous carbon, and rarely explore the mechanisms of material synthesis/fabrication in the carbonization process. In this study, we developed a simple self-template method for preparing N-doped porous carbon from D-calcium pantothenate (DCP). More importantly, the morphology evolution, heteroatom doping, and defect generation of the DCP during carbonization are clarified. Based on a clear understanding of the carbonization mechanism, assisted by DFT theoretical simulations, we find the optimal carbonization temperature of the DCP-derived porous carbon for ZIHCs. The assembled ZIHCs exhibit excellent rate performance (140 mA h g−1 at 0.2 A g−1 and 86.2 mA h g−1 at 6.4 A g−1), and high energy density and power density (111.1 W h kg−1 and 4835.2 W kg−1, respectively). This work provides a constructive avenue for designing high-performance ZIHC electrode materials.
研究领域:
材料科学
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电化学
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能源存储
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纳米材料
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碳材料
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电化学储能
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DOI
高影响因子
Cell Death Discovery
·
2025
·Vol. 11
·期 1
·1-15
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IF 7
Cheng Mengting,Wu Wentong,Li Qing,Tao Xinyu,Jiang Feng,Li Jinjin,Shen Nonger,Wang Fei,Luo Peihua,He Qiaojun,huang Ping,Xu Zhifei,Zhang Yiwen
Sotorasib, the inaugural targeted inhibitor sanctioned for the management of patients afflicted with locally advanced or metastatic non-small cell lung cancer presenting the KRAS G12C mutation, has encountered clinical application constraints due to its potential for cardiac injury as evidenced by safety trials. This investigation has elucidated that the heightened expression of neuraminidase-1 (NEU1) constitutes the principal etiology of cardiac damage induced by sotorasib. Mechanistically, sotorasib treatment inhibited the ubiquitinated degradation of NEU1, leading to its elevated expression, which induced downstream AKT signaling pathway inhibition and mitochondrial dysfunction leading to cardiomyocyte apoptosis. Meanwhile, in vivo and in vitro studies showed that D-pantothenic acid (D-PAC) alleviated sotorasib-induced cardiac damage by promoting NEU1 degradation. In conclusion, this study revealed that NEU1 is a key protein in sotorasib cardiotoxicity and that reducing the level of this protein is a critical strategy for the clinical treatment of sotorasib-induced cardiac injury. Schematic representation of a mechanism.
研究领域:
肿瘤学
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心脏病学
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分子生物学
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药理学
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Junli Li,Guoqiang Xiang,Lijun He,Xiuming Jiang,Renyong Zhao
B, N-co-doped carbon dots (B, N-CDs) were prepared using hydrothermal method with 3-amino phenylboronic acid (3-APBA) as a precursor, yielding 82.5 %. The as-prepared B, N-CDs demonstrated highly stable fluorescence emission with a fluorescence quantum yield (FQY) of 51.4 % under various experimental conditions including 4 M NaCl solution, 120 days of storage, and solution temperature of 100 °C. Based on the boronate affinity between boric acid groups on the surface of B, N-CDs, and the cis -diol structure of ascorbic acid (AA), a novel fluorescence assay for AA was established with a detection limit of 41 nM. Additionally, based on the redox reaction between hydroperoxide (H 2 O 2 ) and boric acid groups on B, N-CDs, a fluorescence assay for H 2 O 2 with a detection limit of 4.5 nM was developed. The proposed fluorescent assay methods were successfully applied for the determination of AA and H 2 O 2 traces in food samples, and the spiking recoveries were in the range of 82.5 % −115.75 %.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
Advanced Science
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2026
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IF 14.1
Kaiyuan Jiang,Fen Xiong,Yiwen Peng,Lingfei Meng,Xinran Wang,Yingruo Xu,Tian Tang,Hongchang Gao
Diabetic cardiomyopathy (DCM) is a major cardiovascular complication of diabetes with limited effective interventions. Using a streptozotocin-induced insulin-deficient, type 1 diabetes-like DCM mouse model, we show that intermittent fasting (IF) improves cardiac function and attenuates myocardial remodeling. Antibiotic-mediated microbiota depletion largely abolished these benefits, whereas fecal microbiota transplantation from IF-treated donors recapitulated cardioprotection, supporting a causal role of the gut microbiota. Metagenomic profiling identified Akkermansia muciniphila ( A. muciniphila ) as a prominent IF-responsive taxon, and A. muciniphila supplementation alleviated cardiac injury without obvious improvement in glycaemia. Integrated serum and heart metabolomics identified 1-methyl-L-histidine as a microbiota-associated metabolite reduced in diabetes but restored by IF and A. muciniphila . In vitro and ex vivo assays further supported an L-anserine-linked microbial route for 1-methyl-L-histidine generation. Importantly, oral 1-methyl-L-histidine supplementation recapitulated key cardioprotective effects, remodeled cardiac lipid homeostasis, and reduced lipid peroxidation and oxidative injury. Together, these findings support a gut microbiota–metabolite–lipid axis associated with IF-related cardioprotection in DCM and highlight microbial metabolites as tractable targets to complement dietary intervention.
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PubMed
高影响因子
Current Nanoscience
·
2025
·Vol. 21
·期 1
·92-98
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IF 1.5
Qiang Wan,Rong Li,Meiping Ren,Gang Ke
Background In the paper, Boletus Brucella was used as carbon source material to prepare carbon dots (CDs) by one-step hydrothermal method. The CDs had high quantum yield and high photostability. Methods A range of characterization studies were conducted on CDs, and the results showed that the average particle size of CDs was 5 nm, emitting blue fluorescence. The optimal excitation wavelength was 337 nm, and the emission wavelength was 440 nm. Results Based on the static quenching, the fluorescence of CDs could be effectively quenched by VB2. Therefore, a highly sensitive and selective fluorescent probe for detecting VB2 was constructed. The CDs were successfully used to detect tablets, human blood, and urine. Conclusion The recovery rate of VB2 was 97.55~99.45%, and the relative standard deviation was 1.29~3.76 (n=3).
研究领域:
生物化学
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分析化学
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材料科学
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
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IF 4.8
Shen Zhang,Zhuo Wang,Yuyu Guo
Although vitamin B12 (VB12) is an essential water-soluble vitamin for the human body, the adverse reaction by excessive use is also worth noting. Therefore, it is crucial to detect the accurate concentration of VB12. In this work, biomass-based carbon dots (BCDs) were prepared by a facile one-pot hydrothermal method from black rice and urea. Based on inner filter effect and static quenching mechanism, the BCDs suggested conspicuous sensitivity and specificity for VB12 detection. The linear ranges were 0.5–100 μM and 0–50 μM under dual fluorescence and colorimetric modes, respectively. Additionally, the obvious color change by addition of VB12 in BCDs solution ensured the feasibility of visual experiments with smartphone assistance. The portable determination system maintained exceptional operability, quick analysis and excellent precision. Combined with the outstanding performance in real samples, this study offers a novel tactic for pharmaceutical analysis based on fluorescence/colorimetric/visualization modes.
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PubMed
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ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Qiqin Wang,Huihui Wu,Kun Peng,Hanying Jin,Huikai Shao,Yuqiang Wang,Jacques Crommen,Zhengjin Jiang
In this research, a hydrophilic polymeric monolith containing choline phosphate (CP) was fabricated through the thermally initiated free-radical polymerization of 2-{2-(methacryloyloxy) ethyldimethylammonium}ethyl n -butyl phosphate (MBP) with ethylene dimethacrylate (EDMA) using isopropanol and tetrahydrofuran as the porogenic system. Excellent mechanical strength, permeability, stability and reproducibility were obtained on the optimized poly(MBP- co -EDMA) monolith. Column efficiency as high as 107,500 plates/m was achieved for the analysis of thiourea at a flow velocity of 0.2 mm/s on the monolith. Both hydrophilic and electrostatic interactions were observed for the retention of charged analytes on the poly(MBP- co -EDMA) monolith. It is worth noting that the resulting monolith exhibits higher selectivity and efficiency than the classical 2-(methacryloyloxy)ethyl phosphorylcholine functionalized polymeric monolith for the chromatographic separation of polar analytes. Also, the novel monolithic column was successfully applied to enrich N -glycopeptides from tryptic digest of human IgG. In a word, the versatile MBP functionalized polymeric monolith not only opens up interesting possibilities for reverse zwitterionic CP derivative-based polymeric materials in separation science , but also represents a promising hydrophilic interaction chromatography stationary phase.
研究领域:
分析化学
·
生物化学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）