ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 15
·3847-3859
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IF 4.157
Xiao Jing,Liu Zhang,Li Chunxiang,Wang Jinglun,Huang Haowen,Yi Qingfeng,Deng Keqin,Li Xiaofang
Graphdiyne (GDY) adsorbed DNA probes have been used as a fluorescent sensing platform, but topics including DNA adsorption affinities, DNA probe displacement, and fluorescence quenching ability were rarely researched. Herein, the adsorption affinity of single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) on a tremella-like GDY was tuned by modulating the surface chemistry of GDY. The fluorescence quenching ability of GDY with different oxidation degrees was compared. The nonspecific displacement of DNA probes on GDY was studied. Under the same concentrations, GDY with low oxidation degree exhibited stronger adsorption affinity and higher adsorption capacity to both ssDNA and dsDNA than highly oxidized GDY. DNA adsorbed on low-oxidized GDY was more resistant to displacement by other DNAs. Protein showed strong interaction with different GDY and could displace DNA probes on GDY. Based on these findings, an ideal GDY with proper oxidation degree, exhibiting high surface affinity for ssDNA and low affinity for dsDNA, was used as scavenger of redundant ssDNA fluorescent probe in an enzyme-assisted amplification system for sensitive ochratoxin (OTA) detection. This study has enhanced our fundamental understanding of DNA adsorption by GDY. It also provided a rational way to apply GDY for fluorescence sensing in a complicated system. Graphical abstract
研究领域:
材料科学
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生物化学
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分析化学
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PubMed
高影响因子
MICROCHIMICA ACTA
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2014
·Vol. 181
·期 9
·1059-1067
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IF 3.719
Zhang Zhihong,Liu Shunli,Kang Mengmeng,Yang Guang,Li Yanshan,Yan Fufeng,He Linghao,Feng Xiaozhong,Wang Peiyuan,Fang Shaoming
We report on a novel nanoarchitecture for use in highly bioactive electrochemical biosensors. It consists of multilayers of nanostructured plasma-polymerized pyrrole (ppPY) and nanosheets of electrically conductive graphene. The ppPY films were deposited by plasma-enhanced chemical vapor deposition on a graphene surface to form nanostructured composites (G-ppPY). The G-ppPY films were then coated with protein (BSA as a model) by adsorption, and then with DNA. The adsorption of protein and DNA on the nanocomposite was studied by electrochemical impedance spectroscopy and with a quartz crystal microbalance. Results demonstrated that the adsorption of biomolecules on G-ppPY films causes a higher variation in its electrochemical properties and adsorbed amount than that on a plain ppPY surface. This indicates that the presence of graphene can enhance the electrochemical activity of ppPY without reducing the sensitivity of biomolecular adsorption.
A novel nanoarchitecture is developed for use in highly bioactive electrochemical biosensors, which is composed of multilayers of nanostructured plasma-polymerized pyrrole and electrically conductive graphene nanosheets. The presence of graphene can enhance the electrochemical activity of ppPY without reducing the sensitivity of biomolecular adsorption.
研究领域:
生物医学工程
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材料科学
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电化学
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DOI
高影响因子
RSC Advances
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2014
·Vol. 4
·期 57
·29968-29974
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IF 3.708
Yongjuan Wang,Yuming Zhou,Tao Zhang,Man He,Xiaohai Bu
This work describes a facile approach for the delaminating of Ni–In-layered double hydroxides (LDHs) by the intercalation of acetate anions in the LDHs gallery using co-precipitation method. The acetate-intercalated Ni–In LDHs exhibited swelling behavior in water and delaminated into semitransparent suspensions on the action of water. The transmission electron microscopy (TEM) and atomic force microscopic (AFM) observations revealed the formation of unilamellar LDHs nanosheets. This simple method did not need reflux at high temperatures, overcoming the deficiency of using organic solvents, resulting in a steady colloidal suspension of 2D nanosheets. The unique properties of 2D ultrathin nanosheets with full access to the positively charged activity sites make them attractive in terms of the improvement of DNA adsorption. The effect of dosage on the adsorption of DNA and the adsorption isotherm were investigated in detail. The equilibrium data were found to be well described by both the Langmuir and Freundlich models. Based on the mechanism of adsorption, the adsorption sites of exfoliated nanosheets can be easily occupied by highly negatively charged small anions through electrostatic interactions. The adsorbed DNA can be released via the addition of a hydrogen phosphate solution.
研究领域:
材料科学
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生物化学
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2018
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IF 2.829
Boyi Niu,Yixian Zhou,Ting Wen,Guilan Quan,Vikramjeet Singh,Xin Pan,Chuanbin Wu
Packaging enough genetic materials is necessary for gene delivery, but functional modification of the carriers may affect their gene loading capacity. In this study, RGD peptide-modified mesoporous silica nanoparticles were synthesized to investigate the influences of functionalization on their DNA adsorption and desorption behaviors. Effects of the functionalization degree of the nanocarriers and the adsorption conditions including DNA concentration, pH, ionic strength and temperature were studied. The DNA adsorption of the nanocarriers were enhanced by reducing pH or adding moderate amounts of ionic salt. Compared with nonfunctional nanoparticles, nanoparticles with low degree of functionalization possessed decreased DNA adsorption capacity but increased adsorption stability. High degree of functionalization resulted in both poor adsorption capacity and stability. Systematic studies showed that the adsorption processes were spontaneous and endothermic, and could be well described by pseudo-second-order kinetic model , Langmuir and Freundlich models. In summary, the DNA loading capacity of mesoporous silica nanoparticles can be improved by proper functionalization and optimized adsorption conditions.
研究领域:
生物材料学
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分子生物学
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纳米医学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2021
·
IF 6.558
Tingting Zhao,Min Zhang,Qi Peng,Xucong Lin,Zenghong Xie
Herein, a facile protocol of simple DNA adsorption on UV-initiated polymerization supports was proposed for effectively fabricating aptamer-based affinity monolithic column. Hydrophilic cationic monolith with an excellent mechanical stability was achieved within 7 min and then massive aptamers were directly bound by DNA charge-dependent adsorption. Strong cationic quaternary ammonium-based monomer was employed to provide effective and stable positive charge surface for aptamer immobilization in a wide range of pH. An ultra-high aptamer coverage density of 6813 pmol/μL was achieved to gain a highly specific online recognition performance. Limitations such as low aptamer capacity, tedious modification and time-consuming reactions in the traditional biological or covalent modification strategies were avoided. By using ochratoxin A (OTA) as the given analyte, the selective recognition and high recoveries were successfully achieved, and little cross-reactivity towards OTB analogue was only 0.5% even if the content of OTB got up to 125 folds of OTA. Applied to sample analysis, the satisfactory discriminations of trace OTA were obtained at 93.9 ± 1.9% – 96.5 ± 1.7%（n = 3）in beer, wheat and chicken liver samples. It might light a cost-effective access to efficiently preparing high-performance affinity monoliths towards the selective in-tube microextraction of OTA.
研究领域:
分析化学
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食品安全
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生物技术
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DOI
PubMed
高影响因子
CRYSTENGCOMM
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2012
·Vol. 15
·期 1
·206-212
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IF 3.842
Xin-Yu Zhao,Ying-Jie Zhu,Feng Chen,Bing-Qiang Lu,Jin Wu
In this paper, a surfactant-free rapid microwave-assisted hydrothermal synthesis of hydroxyapatite nanosheet-assembled flower-like hierarchical nanostructures (NFHNs) is reported. The effects of the experimental conditions on the morphology and crystal phase of the product are investigated. A possible formation mechanism of hydroxyapatite NFHNs is proposed. The morphology of the product can vary from flower-like to polyhedra by adjusting the microwave heating temperature. The protein and DNA adsorption properties of the as-prepared hydroxyapatite NFHNs are studied. The loading capacities of the as-prepared hydroxyapatite NFHNs for bovine serum albumin (BSA), hemoglobin (Hb) and fish sperm DNA are determined to be 165, 164 and 112 mg g−1, respectively. The protein release process is conducted at different pH values (pH 7.2, 5.5 and 4.8) in phosphate buffer saline (PBS), and the pH-controlled protein release behavior has been found. Thus, the as-prepared hydroxyapatite NFHNs are promising for protein drug delivery applications.
研究领域:
材料科学
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生物医学工程
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2015
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IF 4.097
Yanqin Yang,Mengmeng Kang,Shaoming Fang,Minghua Wang,Linghao He,Jihong Zhao,Hongzhong Zhang,Zhihong Zhang
Nanorod-like nanocomposite of three-dimensional reduced graphene oxide and polyaniline (3D-rGO@PANI) was synthesized via an in situ chemical oxidative polymerization method and was then used as the sensitive layer of a DNA adsorbent for detecting Hg 2+ in aqueous solution. Amino-group-rich 3D-rGO@PANI exhibited high affinity toward the immobilization of T-rich DNA strands, which preferred to bind with Hg 2+ to form T Hg 2+ T coordination. Electrochemical impedance spectroscopy was applied to determine the difference in the electrochemical performances during Hg 2+ detection. The results demonstrated that the electrochemical biosensor based on 3D-rGO@PANI nanocomposite showed high sensitivity and selectivity toward Hg 2+ within a concentration range from 0.1 nM to 100 nM with low detection limit of 0.035 nM. The proposed nanosensor could be applied for highly sensitive and selective determination of heavy metal ion in various environmental detections.
研究领域:
分析化学
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环境科学
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纳米生物技术
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电化学传感
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分子生物学
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DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2025
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IF 21.1
Tianyu Wang,Yong Yang,Muyu Li,Panjie Li,Sai Xu,Kan Zhang,Gen Zhang,Jinyou Shen
Antibiotic resistance bacteria (ARB) and antibiotic resistance genes (ARGs) in pharmaceutical wastewater pose serious threats to human health and ecosystems. Traditional disinfection methods have limitations in removing ARB and ARGs, and advanced oxidation processes are hampered by the competition of dissolved organics for reactive species. Herein, we propose a synergistic enhanced adsorption-catalysis strategy by integrating Ti 3 C 2 nanosheets into covalent organic framework (COF) based heterojunction photocatalyst. The excellent electronic conductivity of Ti 3 C 2 promotes electron capture from photoexcited COF, enhancing charge separation and the generation of reactive species. In-situ characterizations confirm Ti-O-P covalent bonding between DNA phosphate skeleton and Ti sites promote ARGs adsorption. This advanced system achieves 99.9 % inactivation of antibiotic-resistant E. coli and 98.9 % simultaneous ARGs degradation, with peak abundance of extracellular ARGs reduced 72.4 % of control without Ti 3 C 2 . In a continious-flow reactor operated for three consecutive 12 h cycles, the prepared photocatalyst realizes consistent inactivation and degradation including ARB, ARGs and antibiotics of pharmaceutical wastewater. This work provides new implications for controlling resistance risk spread during sewage disinfection.
研究领域:
环境科学
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材料科学
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化学工程
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纳米技术
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水处理
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微生物学
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遗传学
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DOI
高影响因子
RSC Advances
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2020
·Vol. 10
·期 51
·31012-31021
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IF 3.119
Shengmei Wang,Linqi Ouyang,Guiming Deng,Zhenzhen Deng,Shengfeng Wang
DNA functionalized nanomaterials have attracted tremendous attention for bioanalytical applications. Owing to exceptional fluorescence quenching ability, most DNA-based nanoprobes were designed with turn-on signals for target gene detection, while only a few of them could simultaneously achieve gene detection and regulation in one system. In this study, we explored the use of nanoscale zeolitic imidazolate framework-8 (ZIF-8) as a building block to construct a DNA-based nanoprobe. We found ZIF-8 could stably adsorb DNA to resist the dissociation by various biological ligands, enabling potential biological applications. However, ZIF-8 was not a nano-quencher to turn off the fluorophore labeling on the adsorbed DNA. We therefore designed a DNAzyme embedded molecular beacon (DMB) to functionalize ZIF-8. After endocytosis, ZIF-8 was disintegrated to release DMB for target mRNA detection, and the co-released Zn2+ acted as an effective cofactor to activate the embedded DNAzyme for mRNA regulation. This study provides a versatile nano-platform to realize multiple functions inside cells by using functional nucleic acids, which holds great promise for theranostic applications.
研究领域:
生物化学
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纳米技术
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分子生物学
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生物医学工程
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2016
·
IF 7.145
Jing Sun,Dennis Curry,Qipeng Yuan,Xu Zhang,Hao Liang
Gold nanoparticle (AuNP)-templated spherical nucleic acids (SNAs) have been demonstrated as an important functional material in bionanotechnology. Fabrication of SNAs having high hybridization capacity to their complementary sequences is critical to ensure their applicability in areas such as antisense gene therapy and cellular sensing. The traditional salt-aging procedure is effective but tedious, requiring 1–3 days to complete. The rapid low-pH assisted protocol is efficient, but causes concerns related to nonspecific DNA adsorption to the AuNP core. To address these issues, we systematically compared the SNAs prepared by these two methods (salt-aging method and low-pH protocol). In terms of the number of complementary DNA that each SNA can bind and the average binding affinity of each thiolated DNA probe to its complementary strand, both methods yielded comparable hybridizability, although higher loading capacity was witnessed with SNAs made using the low-pH method. Additionally, it was found that nonspecific DNA binding could be eliminated almost completely by a simple glutathione (GSH) treatment of SNAs. Compared to conventional methods using toxic mercapto-hexanol or alkanethiols to remove nonspecific DNA adsorption, GSH is mild, cost-effective, and technically easy to use. In addition, GSH-passivated SNAs minimize the toxicity concerns related to AuNP-induced GSH depletion and therefore offer a more biocompatible alternative to previously reported SNAs. Moreover, rational design of probe sequences through inclusion of a polythymine spacer into the DNA sequences resulted in enhanced DNA loading capacity and stability against salt-induced aggregation. This work provides not only efficient and simple technical solutions to the issue of nonspecific DNA adsorption, but also new insights into the hybridizability of SNAs.
研究领域:
生物纳米技术
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纳米医学
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分子生物学
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材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2025
·Vol. 30
·期 17
·3551
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IF 4.6
Senhao Hu,Wenjing Wang,Yu Zou,Chunmei Li,Hongyan Zou,Chengzhi Huang,Lei Zhan
The development of low-background, facile, and robust fluorescent nanoprobes for imaging and monitoring of intracellular mRNA changes remains a great challenge. Taking advantage of the high fluorescence quenching efficiency of core-shell gold@polydopamine (Au@PDA) nanocomposites and Ca 2+ -promoting DNA adsorption stability, a simple and universal bioconjugate strategy was designed to a construct fluorescent nanoprobe for highly efficient tumor-related mRNA imaging. The fluorescence of Cy5-labeled DNA was quenched up to 92.38% by the AuNP and PDA via nanometal surface energy transfer (NSET) and photoinduced electron transfer (PET), respectively. TK1 mRNA, a biomarker of tumor growth, initiates hybridization and results in fluorescence recovery, which built the foundation for identifying the expression level changes in living cells. More importantly, three kinds of tumor-related mRNA (TK1 mRNA, GalNAc-T mRNA, and C-myc mRNA) can be detected simultaneously with different fluorophore-modified recognition sequences, which can avoid false positive signals and improve the reliability of cancer diagnostic, holding great promise for cancer diagnosis, prognosis, and therapy.
研究领域:
纳米生物技术
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分子成像
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癌症诊断
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生物分析化学
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基因表达监测
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DOI
PubMed
高影响因子
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