ANALYTICA CHIMICA ACTA
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2024
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IF 5.7
Zhiyong Yin,Zhibing Zhang,Xili Jing,Linchuan Hu,Shuguang Li,Jianshe Li
Background Surface plasmon resonance (SPR) sensing technology has been widely used in biometrics, but the weak detection capability and low sensitivity limit the development of SPR biosensors. In this work, we propose to employ the transition metal disulfide (TMD) material MoS 2 to induce the SPR effect into the near-infrared band. The aim of this work is to develop a near-infrared sensor capable of quantitatively detecting the concentration of cDNA, which is able to solve the problems of low sensitivity, parameter crosstalk and so on. Results The results show that the sensitivity of the SPR sensor at infrared wavelengths is 1.69 times higher than that of visible, and the infrared wave is more suitable as an excitation source for the SPR effect because it has a stronger evanescent field. In addition, we have prepared a DNA hybridization sensor that can work in the near-infrared band to detect complementary DNA (cDNA) concentration. Moreover, a combined cascade and parallel strategy realized the temperature and PH detection. The sensor uses the photonic crystal fiber as the platform, silver film as an excitation layer, and MoS 2 as a modulation layer. The optimal parameters of the sensor were determined during the experiment, and the three-parameter detection was successfully realized. The experimental results show that the three sensing channels can work independently, and the DNA hybridization probe can achieve selective detection of cDNA with a sensitivity as high as 0.22 nm/(nmol/L). Significance The proposed three-channel DNA hybridization sensor has high sensitivity and accuracy. The innovation of this work is to demonstrate that infrared waves are more suitable to be used as the excitation source of SPR sensors and that the proposed three-channel sensor has a promising future in early diagnosis and biosensing.
研究领域:
生物传感
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分子生物学
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光学工程
✓ 已验证
DOI
高影响因子
APL Photonics
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2024
·Vol. 9
·期 9
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IF 5.4
Yin Zhiyong,Jing Xili,Li Shuguang
As an essential biomarker for diagnosing and treating various diseases, low-cost, quantitative detection methods for complementary DNA (cDNA) have received much attention. The surface plasmon resonance (SPR) sensing technique is an effective measurement scheme, but the ambient temperature and pH variations have a non-negligible impact. In this work, we developed a triple-probe SPR sensing system for detecting cDNA concentration, temperature, and pH. In order to satisfy the triple parameter measurements, we used a microstructured optical fiber as the sensing platform, silver and gold films as the excitation layer, and a MoS 2 film as the modulation layer. First, we explore the modulation mechanism of SPR and the conditions for excitation of triple SPR and demonstrate that the carrier concentration is a crucial factor affecting the resonance wavelength. Then, the feasibility of the sensing system for triple-probing is theoretically analyzed. Finally, in the experiment, the optimal parameters of the sensor were determined, and the triple parameter detection was successfully realized. The experimental results show that the three probes can work independently, and the hybridized DNA probe can realize the selective detection of cDNA with a sensitivity of 0.249 nm/(nmol/l). The maximum sensitivity of the pH probe and the temperature probe are 51.5 nm/pH and 6.14 nm/°C. In addition, the experimental results show that the sensing probes have excellent reproducibility. This paper’s innovation is using the fiber optic SPR effect to achieve quantitative detection for cDNA, temperature detection, and pH detection. Therefore, the sensor has a promising future in early diagnosis and biosensing.
研究领域:
生物医学工程
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分子诊断
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纳米技术
✓ 已验证
DOI
高影响因子
CARBON
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2019
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IF 7.466
Yi Yu,QingYi Zhu,Feifan Xiang,Yue Hu,Lijie Zhang,Xiangju Xu,Nannan Liu,Shaoming Huang
AuNPs/SWCNT, a hybrid nanomaterial which is single-walled carbon nanotube (SWCNT) modified with gold nanoparticles (AuNPs), has great application prospects for chemical and biological sensors. For the innate nanogap between tiny AuNPs deposited on sidewall of SWCNT, AuNPs/SWCNT is suitable for the fabrication of simple and effective electrical signal switching devices. Here, we develop a novel strategy of applying AuNPs/SWCNT to fabricate nanogap device for DNA hybridization detection. The detection strategy is based on the target DNA mediating the catalytic activity of the AuNPs by desorbing the probe DNA from AuNPs. The signal transduction depends on the growth of tiny AuNPs which is closely related to the AuNPs catalytic activity. Sensing occurs at the tiny AuNPs growing and connecting into a continuous nanowire along the SWCNT, leading to a conductimetric response. The biosensing device can detect target DNA with the detection limit of 1 pM and the ability to discriminate triple and quintuple base pair mismatches.
研究领域:
生物传感器
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纳米技术
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
CARBON
·
2021
·
IF 9.594
Yang Sun,Shicai Xu,Tiying Zhu,Jiajun Lu,Shuo Chen,Maomao Liu,Guangcan Wang,Baoyuan Man,Huamin Li,Cheng Yang
Field-effect transistors (FET) using low-dimensional materials has attracted attention for detection of DNA hybridization. Here, we proposed a photocurrent-modulated FET sensor, where the I p h o t o c u r r e n t ( g a t e ) + I p h o t o c u r r e n t ( D N A ) dramatically enhance the response signal, I c u r r e n t , compared to a traditional FET sensor. The proposed sensor successfully detects DNA signals with a limit of detection (LOD) down to attomolar concentrations that is nearly 100x lower than a traditional FET sensor. The sensing mechanism is postulated and discussed. The results suggest that photo-coupled modulation opens new avenues for further development of the FET in biosensing applications.
研究领域:
生物传感器
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纳米材料
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分子诊断
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Xu-guang Hu,Yong Zhao,Yun Peng,Rui-jie Tong,Hong-kun Zheng,Jian Zhao,Sheng Hu
An in-fiber optofluidic Michelson interferometer fabricated by femtosecond laser micromachining technology was presented. The interferometer structure was prepared by a special optical fiber with two cores and a microchannel . One core was connected to the core of a single-mode fiber (SMF) and functioned as a sensing arm on the microchannel inner wall, the other core worked as a reference arm and was connected to the optical waveguide introduced by femtosecond laser processing. By combining with a ring cavity laser, the detection limit (DL) of the interferometer could be improved. The laser sensor presented a high linear response with a refractive index (RI) sensitivity of 1039.77 nm/RIU and the calculated DL could reach as low as 1.675 × 10 −5 RIU. Then, the sensor measured different concentrations of vitamin C in tablets in the concentration range of 0–10 mg/mL with a detection limit of 0.06 mg/mL. Due to the advantages of optofluidic structure, the volume of the detection solution could be as low as few tens of nanoliters, and the time to replace and detect the solution only took a few seconds. In addition, the laser sensor realized label-free DNA hybridization detection through surface functionalization , and could detect 1 μM target DNA solution with high specificity. This sensing device had great potential for small volume and low concentration sample detection in the biochemical field.
研究领域:
光学
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生物医学工程
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化学传感
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Yaping Xia,Yang Sun,Huamin Li,Shuo Chen,Tiying Zhu,Guangcan Wang,Baoyuan Man,Jie Pan,Cheng Yang
Room-temperature plasma treated graphene based FET was firstly proposed for the DNA hybridization detection. Affinity and electrical properties of the graphene based DNA-FET sensor were studied and improved benefits from the surface modification. The facile room-temperature Ar plasma easily removes residues from the graphene surface and changes the hydrophilic properties of graphene, which is important for our solution gated DNA-FET sensor. Limit of the detection of below 10 aM is obtained in our experiment. Especially, DNA concentration (C DNA )/the amount of net drain current (ΔI) and the negative shift in the V CNP value of the GFET sensor with the plasma treated 30 s are all improved compared with that without treatment. It shows that the easily plasma treatment of the graphene surface can be used for the solution gated FET sensor.
研究领域:
材料科学
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生物传感器
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纳米技术
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电子工程
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）