MICROCHIMICA ACTA
·
2011
·Vol. 175
·期 3
·355-359
·
IF 2.578
Lu Wenbo,Qin Xiaoyun,Luo Yonglan,Chang Guohui,Sun Xuping
We demonstrate that CdS quantum dots (QDs) can be applied to fluorescence-enhanced detection of nucleic acids in a two-step protocol. In step one, a fluorescently labeled single-stranded DNA probe is adsorbed on the QDs to quench its luminescence. In step two, the hybridization of the probe with its target ssDNA produces a double-stranded DNA which detaches from the QD. This, in turn, leads to the recovery of the fluorescence of the label. The lower detection limit of the assay is as low as 1 nM. The scheme (that was applied to detect a target DNA related to the HIV) is simple and can differentiate between perfectly complementary targets and mismatches.
CdS quantum dots (CdSQDs) can serve as an effective sensing platform for fluorescence-enhanced DNA detection. This sensing system has a detection limit of 1 nM and is capable of differentiating between complementary and mismatched sequences.
研究领域:
生物化学
·
分子生物学
·
纳米技术
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Zhiyong Yin,Zhibing Zhang,Xili Jing,Linchuan Hu,Shuguang Li,Jianshe Li
Background Surface plasmon resonance (SPR) sensing technology has been widely used in biometrics, but the weak detection capability and low sensitivity limit the development of SPR biosensors. In this work, we propose to employ the transition metal disulfide (TMD) material MoS 2 to induce the SPR effect into the near-infrared band. The aim of this work is to develop a near-infrared sensor capable of quantitatively detecting the concentration of cDNA, which is able to solve the problems of low sensitivity, parameter crosstalk and so on. Results The results show that the sensitivity of the SPR sensor at infrared wavelengths is 1.69 times higher than that of visible, and the infrared wave is more suitable as an excitation source for the SPR effect because it has a stronger evanescent field. In addition, we have prepared a DNA hybridization sensor that can work in the near-infrared band to detect complementary DNA (cDNA) concentration. Moreover, a combined cascade and parallel strategy realized the temperature and PH detection. The sensor uses the photonic crystal fiber as the platform, silver film as an excitation layer, and MoS 2 as a modulation layer. The optimal parameters of the sensor were determined during the experiment, and the three-parameter detection was successfully realized. The experimental results show that the three sensing channels can work independently, and the DNA hybridization probe can achieve selective detection of cDNA with a sensitivity as high as 0.22 nm/(nmol/L). Significance The proposed three-channel DNA hybridization sensor has high sensitivity and accuracy. The innovation of this work is to demonstrate that infrared waves are more suitable to be used as the excitation source of SPR sensors and that the proposed three-channel sensor has a promising future in early diagnosis and biosensing.
研究领域:
生物传感
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分子生物学
·
光学工程
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Mengjing Cui,Qiuyue Zhao,Qi Zhang,Mingxuan Fu,Yuexian Liu,Xinyu Fan,Haiyang Wang,Yufan Zhang,Huan Wang
In the interest of developing novel electrocatalyst for high performance DNA biosensing, with distinctive chiral double helix nanostructure , nitrogen doped chiral carbonaceous nanotube (Chiral-CNT) was employed for ultrasensitive label-free DNA biosensing research. Chiral-CNT can quantitative detection of four DNA bases with high sensitivity and selectivity . Without any prehydrolysis and labeling process, direct electrochemistry of single-stranded DNA and double-stranded DNA, qualitative and quantitative detection of DNA hybridization (low detection limit: 0.0268 g L −1 ) were realized. Moreover, sensitive detection of DNA damage induced by fenton reagent was also realized with low detection limit of 0.0350 mg mL −1 and high sensitivity of 7.42 μA mg −1 mL. The high biosensing performance attributes to the unique chiral structure of Chiral-CNT, leads to efficient interreaction between Chiral-CNT and DNA molecule.
研究领域:
生物传感器
·
电化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Xuegang Li,Pengqi Gong,Xue Zhou,Shankun Wang,Yingxuan Liu,Yanan Zhang,Linh V. Nguyen,Stephen C. Warren-Smith,Yong Zhao
An advanced multi-parameter optical fiber sensing technology for EGFR gene detection based on DNA hybridization technology is demonstrated in this paper. For traditional DNA hybridization detection methods, temperature and pH compensation can not be realized or need multiple sensor probes. However, the multi-parameter detection technology we proposed can simultaneously detect complementary DNA, temperature and pH based on a single optical fiber probe. In this scheme, three optical signals including dual surface plasmon resonance signal (SPR) and Mach-Zehnder interference signal (MZI) are excited by binding the probe DNA sequence and pH-sensitive material with the optical fiber sensor. The paper proposes the first research to achieve simultaneous excitation of dual SPR signal and Mach-Zehnder interference signal in a single fiber and used for three-parameter detection. Three optical signals have different sensitivities to the three variables. From a mathematical point of view, the unique solutions of exon-20 concentration, temperature and pH can be obtained by analyzing the three optical signals. The experimental results show that the exon-20 sensitivity of the sensor can reach 0.07 nm nM −1 , and the limit of detection is 3.27 nM. The designed sensor gives a fast response, high sensitivity, and low detection limit, which is important for the field of DNA hybridization research and for solving the problems of biosensor susceptibility to temperature and pH.
研究领域:
生物医学工程
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2010
·
IF 3.325
Xianggang Liu,Xiangjin Qu,Hai Fan,Shiyun Ai,Ruixia Han
An electrochemical method for the detection of DNA hybridization using a novel electroactive, cationic, and water-soluble branched polyethyleneimine (BPEI)–cobalt(III)–phenanthroline(phen) polymeric indicator and single-stranded neutral peptide nucleic acid (PNA) probe on the Au electrode was developed. The indicator possesses some free amine groups, as well as cationic cobalt complexes in the polymer chain. It does not bind to neutral PNA capture probe alone. However, the indicator strongly interacts with the negatively charged backbone of the complementary oligonucleotide bound to the PNA probe through electrostatic interactions . The coordination spherical moieties also interact with the probe by embedding into the double-helix structure of PNA–DNA. These two interactions enable transduction of hybridization, producing a clear electrical signal in differential pulse voltammetry (DPV). The correlation against non-complementary DNA, three-base and one-base mismatch DNA was sharp, and the signal of indicator for the target DNA demonstrated a linear relationship within the concentration range of 5.0 × 10 −9 to 2.5 × 10 −7 M ( R = 0.9940) with a detection limit of 5.6 × 10 −10 M. These studies showed that the novel polymeric indicator and single-stranded PNA probe could be used to fabricate an electrochemical biosensor for DNA detection. This technique can provide an alternative route for expanding the range of detection methods available for DNA hybridization.
研究领域:
生物化学
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分析化学
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分子生物学
✓ 已验证
DOI
高影响因子
APL Photonics
·
2024
·Vol. 9
·期 9
·
IF 5.4
Yin Zhiyong,Jing Xili,Li Shuguang
As an essential biomarker for diagnosing and treating various diseases, low-cost, quantitative detection methods for complementary DNA (cDNA) have received much attention. The surface plasmon resonance (SPR) sensing technique is an effective measurement scheme, but the ambient temperature and pH variations have a non-negligible impact. In this work, we developed a triple-probe SPR sensing system for detecting cDNA concentration, temperature, and pH. In order to satisfy the triple parameter measurements, we used a microstructured optical fiber as the sensing platform, silver and gold films as the excitation layer, and a MoS 2 film as the modulation layer. First, we explore the modulation mechanism of SPR and the conditions for excitation of triple SPR and demonstrate that the carrier concentration is a crucial factor affecting the resonance wavelength. Then, the feasibility of the sensing system for triple-probing is theoretically analyzed. Finally, in the experiment, the optimal parameters of the sensor were determined, and the triple parameter detection was successfully realized. The experimental results show that the three probes can work independently, and the hybridized DNA probe can realize the selective detection of cDNA with a sensitivity of 0.249 nm/(nmol/l). The maximum sensitivity of the pH probe and the temperature probe are 51.5 nm/pH and 6.14 nm/°C. In addition, the experimental results show that the sensing probes have excellent reproducibility. This paper’s innovation is using the fiber optic SPR effect to achieve quantitative detection for cDNA, temperature detection, and pH detection. Therefore, the sensor has a promising future in early diagnosis and biosensing.
研究领域:
生物医学工程
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分子诊断
·
纳米技术
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-7
·
IF 5.479
Xu Qiang,Gong Yan,Zhang Zhifeng,Miao Yanming,Li Dongxia,Yan Guiqin
A one-pot hydrothermal method was developed for the synthesis of graphene oxide quantum dots (GOQDs). It is making use of toner waste as the precursor and H 2 O 2 as the oxidant. Synthesis takes 4 h and does not require strong acids or complex purification steps and does not produce environmentally harmful metal ions. The GOQDs display blue fluorescence with excitation/emission maxima at 340/445 nm. The feasibility of detecting specific DNA sequence was promoted using polyethyleneimine to modify the GOQDs surface. A method was developed to recognized a specific DNA sequence. This is based on electrostatic aggregation of GOQDs and ssDNA labeled with Dabcyl at the 3′ end, which promotes fluorescence quenching of GOQDs. The possible fluorescence quenching mechanism (which is mainly dynamic) was investigated using the Stern-Volmer equation. When a target sequence was added, which is complementary to the ssDNA, the dabcyl-labeled ssDNA is released due to strict complementary base pairing. This promotes fluorescence recovery of GOQDs. The assay has a 0.17 nM detection limit and a linear range of 0.5–30 nM. The method was used to quantify specific DNA sequences from extracts of genetically modified plant tissues.
Graphene oxide quantum dots (GOQDs) were synthesized by one-pot hydrothermal method using waste toner, and the surface was modified by polyethyleneimine (PEI). Through the interaction of PEI-GOQDs with Dabcyl-DNA single strands to dynamically quench the fluorescence of GOQDs. Based on DNA hybridization technology, we established specific DNA sequence detection nanoprobe.
研究领域:
生物化学
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环境科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBON
·
2019
·
IF 7.466
Yi Yu,QingYi Zhu,Feifan Xiang,Yue Hu,Lijie Zhang,Xiangju Xu,Nannan Liu,Shaoming Huang
AuNPs/SWCNT, a hybrid nanomaterial which is single-walled carbon nanotube (SWCNT) modified with gold nanoparticles (AuNPs), has great application prospects for chemical and biological sensors. For the innate nanogap between tiny AuNPs deposited on sidewall of SWCNT, AuNPs/SWCNT is suitable for the fabrication of simple and effective electrical signal switching devices. Here, we develop a novel strategy of applying AuNPs/SWCNT to fabricate nanogap device for DNA hybridization detection. The detection strategy is based on the target DNA mediating the catalytic activity of the AuNPs by desorbing the probe DNA from AuNPs. The signal transduction depends on the growth of tiny AuNPs which is closely related to the AuNPs catalytic activity. Sensing occurs at the tiny AuNPs growing and connecting into a continuous nanowire along the SWCNT, leading to a conductimetric response. The biosensing device can detect target DNA with the detection limit of 1 pM and the ability to discriminate triple and quintuple base pair mismatches.
研究领域:
生物传感器
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纳米技术
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2014
·Vol. 6
·期 15
·8681-8693
·
IF 6.739
Xuefan Gu,Youshen Wu,Lingze Zhang,Yongchun Liu,Yan Li,Yongli Yan,Daocheng Wu
To improve the metal-enhanced fluorescence (MEF) effect of nanogolds (AuNPs) and accurately detect specific DNA sequences via DNA hybridization, novel hybrid magnetic nanoparticles/nanogold clusters (HMNCs) were designed based on finite-difference time-domain simulation results and prepared by using Fe3O4 and nanogolds. The nanogolds outside the HMNC were then conjugated with thiol-terminated DNA molecules, thus DNA modified-HMNCs (DNA–HMNCs) were obtained. The size distributions of these nanostructures were measured by a Malvern size analyzer, and their morphology was observed via transmission electron microscopy (TEM). The ultraviolet (UV)-visible (vis) absorption spectra of the samples were recorded with a UV-2600 spectrophotometer. Fluorescence spectra and the MEF effect were recorded using a spectrophotofluorometer, and lifetimes were determined using a time-correlated single photon counting apparatus. The prepared HMNCs were stable in aqueous solutions and had an average diameter of 87 ± 3.2 nm, with six to eight AuNPs around a single Fe3O4 nanoparticle. Fluorescein isothiocyanate (FITC) tagged DNA–HMNC conjugates exhibited a significant MEF effect and could accurately detect specific DNA sequences after DNA hybridization. This result indicates their various potential applications in sensors and biomedical fields.
研究领域:
生物化学
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纳米医学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2017
·
IF 2.334
Zhi Li,Bingchen Li,Yunlei Zhou,Huanshun Yin,Jun Wang,Shiyun Ai
It is extremely important for quantifying trace microRNAs in the biomedical applications. In this study, an ultrasensitive, rapid and efficient label-free fluorescence method was proposed and applied for detecting microRNA-21 in serum of gastric cancer patients based on DNA hybridization chain reaction (HCR). DNA H1 and DNA H2 were designed and used as hairpin probes, the HCR was proceeded in the presence of target microRNAs. Amounts of SYBR Green І dyes were used as signal molecules to intercalate long DNA concatemers from HCR, which guaranteed the model of label-free fluorescence and strong fluorescence density. The detection method showed a wide linear region from 1 fM to 10 5 fM, and the limit of detection was 0.2554 fM (at S/N = 3) for microRNAs. The results showed that this method had an excellent specificity and reproducibility. Furthermore, the label-free fluorescence strategy exhibited a sensitive response to microRNA-21 in real serum samples of gastric cancer patients and the results obtained were in accordance with reference method (R 2 = 0.994). Overall, the proposed strategy could be satisfactory for rapid, ultrasensitive and efficient detection of microRNA-21, and held great potentials in clinic diagnosis of gastric cancer.
研究领域:
生物技术
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医学诊断
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2011
·Vol. 136
·期 19
·3973-3980
·
IF 3.913
Feng Gao,Peng Cui,Xiaoxiao Chen,Qingqing Ye,Maoguo Li,Lun Wang
A novel and efficient method to evaluate the DNA hybridization based on a fluorescence resonance energy transfer (FRET) system, with fluorescein isothiocyanate (FITC)-doped fluorescent silica nanoparticles (SiNPs) as donor and gold nanoparticles (AuNPs) as acceptor, has been reported. The strategy for specific DNA sequence detecting is based on DNA hybridization event, which is detected via excitation of SiNPs-oligonucleotide conjugates and energy transfer to AuNPs-oligonucleotide conjugates. The proximity required for FRET arises when the SiNPs-oligonucleotide conjugates hybridize with partly complementary AuNPs-oligonucleotide conjugates, resulting in the fluorescence quenching of donors, SiNPs-oligonucleotide conjugates, and the formation of a weakly fluorescent complex, SiNPs-dsDNA-AuNPs. Upon the addition of the target DNA sequence to SiNPs-dsDNA-AuNPs complex, the fluorescence restores (turn-on). Based on the restored fluorescence, a homogeneous assay for the target DNA is proposed. Our results have shown that the linear range for target DNA detection is 0–35.0 nM with a detection limit (3σ) of 3.0 picomole. Compared with FITC-dsDNA-AuNPs probe system, the sensitivity of the proposed probe system for target DNA detection is increased by a factor of 3.4-fold.
研究领域:
生物化学
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纳米生物学
·
分子诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBON
·
2021
·
IF 9.594
Yang Sun,Shicai Xu,Tiying Zhu,Jiajun Lu,Shuo Chen,Maomao Liu,Guangcan Wang,Baoyuan Man,Huamin Li,Cheng Yang
Field-effect transistors (FET) using low-dimensional materials has attracted attention for detection of DNA hybridization. Here, we proposed a photocurrent-modulated FET sensor, where the I p h o t o c u r r e n t ( g a t e ) + I p h o t o c u r r e n t ( D N A ) dramatically enhance the response signal, I c u r r e n t , compared to a traditional FET sensor. The proposed sensor successfully detects DNA signals with a limit of detection (LOD) down to attomolar concentrations that is nearly 100x lower than a traditional FET sensor. The sensing mechanism is postulated and discussed. The results suggest that photo-coupled modulation opens new avenues for further development of the FET in biosensing applications.
研究领域:
生物传感器
·
纳米材料
·
分子诊断
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）