Song Yiwei,Jin Xiuyan,Zhao Yiou,Cheng Shuwen,Xu Sai,Bu Shengjun,Liu Liming,Zhou Chunyang,Pang Chunying
Single-level biomarker detection has the limitation of insufficient accuracy in cancer diagnosis. Therefore, the strategy of developing highly sensitive, multi-channel biosensors for high-throughput ctDNA determination is critical to improve the accuracy of early diagnosis of clinical tumors. Herein, in order to achieve efficient detection of up to ten targets for early diagnosis of ovarian cancer, a DNA-nanoswitch-based multi-channel (DNA-NSMC) biosensor was built based on the multi-module catalytic hairpin assembly-mediated signal amplification (CHA) and toehold-mediated DNA strand displacement (TDSD) reaction. Only two different fluorescence signals were used as outputs, combined with modular segmentation strategy of DNA-nanoswitch-based reaction platform; the multi-channel detection of up to ten targets was successfully achieved for the first time. The experimental results suggest that the proposed biosensor is a promising tool for simultaneously detecting multiple biomarkers for the early diagnosis of ovarian cancer, offering new strategies for the early screening, diagnosis, and treatment not only for ovarian cancer but also for other cancers. Graphical abstract
Yuan Yuan,Mengqin Zhang,Guoyang Xie,Jianxiu Wang,Fan Wu,Xinyao Yi
The fabrication of DNA-based nanomaterials with specific functionalities is crucial for advancing tumor therapy. Herein, a novel and facile approach was presented to construct a corona-shaped DNA hybrid nanoplatform, which involved the anchoring of rolling circle amplification (RCA) products on near-infrared (NIR) light-responsive gold nanoflowers (AuNFs). The incorporation of AS1411 aptamer into the RCA product endowed the nanoplatform with the capability of multivalent targeted binding to cancer cells. The nanoplatform exhibited good biocompatibility, notable NIR absorption and robust photothermal conversion, thereby enabling controlled drug release in response to dual NIR/pH stimulation. The developed nanoplatform holds great promise for targeted and responsive cancer treatments.
危险品化学品经营许可证（带存储）