ANALYTICAL BIOCHEMISTRY
·
2020
·
IF 2.877
Yongquan Liu,Ahui Xue,Siyu Wang,Yingbin Hao,Meng Cui,Lihua Liu,Liping Luo
Asian citrus psyllid (ACP) causes direct and indirect damage to the citrus industry. Extractive electrospray ionization mass spectrometry (EESI-MS) and high performance liquid chromatography (HPLC) were used to detect the metabolites of C. limon leaves at 0, 12, 24, and 72 h after ACP treatment. The EESI-MS results showed that ACP infestation significantly affected metabolites within a short feeding duration with 8 metabolites identified. The metabolites in leaves of these four groups could be distinguished, with 55 peaks showing significant differences including methyl N -methylanthranilate, caffeic acid , and syringic acid . The quantification of 15 phenolic compounds with HPLC-UV method in C. limon leaves after ACP infestation showed that the total content of them reached a peak of 3504.69 μg g −1 at 12 h, with 9 phenolic compounds changing significantly ( P < 0.05). A total of 21 metabolites identified in this study were involved in the biosynthesis pathways of flavonoid , flavone and flavonol , isoflavonoid and phenylpropanoid , and the degradation of aminobenzoate . Contents of epicatechin and caffeic acid increased with the feeding time of ACP as detected by both EESI-MS and HPLC. This may be related to plant defense. This study provides novel insights into the biochemical relationship of ACP and its host plants.
研究领域:
植物学
·
昆虫学
·
代谢组学
✓ 已验证
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2021
·
IF 3.365
Ahui Xue,Yongquan Liu,Haoxing Li,Meng Cui,Xueyong Huang,Wenjing Wang,Dong Wu,Xiali Guo,Yingbin Hao,Liping Luo
Huanglongbing (HLB), a devastating disease for citrus worldwide, is caused by Candidatus Liberibacter asiaticus ( C Las). In this study, we employed a novel extractive electrospray ionization-mass spectrometry (EESI-MS) method to analyze the metabolites in leaves of uninfected and HLB-infected Newhall navel orange. The results showed that uninfected and HLB-infected leaves could be readily distinguished based on EESI-MS combined by multivariable analysis. Nine phenolic compounds involved in phenylpropanoid pathway, such as p -coumaric acid, naringin , and apigenin , were principal components to distinguish the leaves of uninfected and HLB-infected Newhall navel orange. Gene expression was also conducted to further explore the molecular mechanism of phenylpropanoid branch pathway in HLB. The expression of genes ( 4CL , HCT , CHI , CHS , CYP , and C12R ) involved in phenylpropanoid branch pathway was increased in asymptomatic and early period of HLB-infected leaves, while decreased in later period of HLB-infected leaves. This study provides a novel method for early detection of citrus HLB and suggests the regulation mechanism of phenylpropanoid pathway in the interaction between citrus and C Las.
研究领域:
植物病理学
·
分子生物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Shangguang Du,Xutang Tu,Xiaohua Duan,Hao Wan,Zuozuo Ai,Jun Luo,Zhengrong Zou,Liping Luo
Propolis exhibits significant anti-inflammatory, antidiabetic, and antiobesity properties in both mouse models and clinical applications. However, the underlying metabolic mechanisms remain poorly understood. Traditional metabolomic methods that rely on chromatographic separation require complex preprocessing steps and extended detection periods. In this study, we employed extraction electrospray ionization mass spectrometry combined with multivariate analysis to directly profile metabolites in the colon tissue of mice. Our findings demonstrate the efficacy of ethanol extract of propolis (EEP) in mitigating weight gain, reducing inflammatory cytokines, and improving insulin resistance induced by a high-fat diet. Additionally, EEP enhanced glucose tolerance. Through collision-induced dissociation experiments, we identified 26 metabolites, with 4-hydroxyphenylacetic acid, protocatechuic acid, caffeic acid, ferulic acid, hippuric acid, histidine, and tryptophan emerging as potential biomarkers. Notably, tryptophan exhibited the highest content at 8.25 mg/g. Our research facilitates rapid profiling of colon metabolites, underscoring its significant potential for broader applications in animal metabolomic studies.
研究领域:
代谢组学
·
药理学
·
营养学
·
动物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Dong Wu,Huihui Ming,Wei Wu,Wenjie Yu,Gaowei Zhang,Jinzhu Gu,Meng Cui,Xueyong Huang,Fangjian Ning,Liping Luo
Light quality affects plant leaf growth and its metabolism of amino acids and polyphenols. Anoectochilus roxburghii has been used for food , medicine, and ornamental purposes. To obtain the optimal light quality for improved accumulation of metabolites in A. roxburghii in vitro . The seedings were cultured under the white light (W), blue light (B), red light (R), red: blue light = 1:2 (R1B2), red: blue light = 1:1 (R1B1), red: blue light = 2:1 (R2B1). Twenty compounds in raw leaves were in situ rapidly identified within 20 s by neutral desorption-extractive electrospray ionization mass spectrometry (ND-EESI-MS) without any sample pretreatment, including eight amino acids , eleven polyphenols, and kinsenoside. Meanwhile, leaf area and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were evaluated. Our results showed that leaf area and antioxidant enzyme activities of the R2B1 were significantly higher than B or R group. The red-blue light increased the content of isoleucine , histidine , serine , arginine, and promoted the accumulation of caffeic acid , ferulic acid , quercetin , myricetin , kaempferol , and rutin in phenylpropane metabolism, especially in the R2B1, which could be used as the optimal light quality. ND-EESI-MS could enhance our understanding of the effect of light quality on the accumulation of amino acids and polyphenols in A. roxburghii .
研究领域:
植物学
·
代谢组学
·
药用植物研究
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2014
·Vol. 406
·期 29
·7705-7714
·
IF 3.578
Huang X. Y.,Fang X. W.,Zhang X.,Dai X. M.,Guo X. L.,Chen H. W.,Luo L. P.
Herein, we constructed a platform of neutral desorption-extractive electrospray ionization mass spectrometry (ND-EESI-MS) for direct and rapid detection of chloramphenicol (CAP) in honey samples diluted with methanol. Under the optimized working conditions, the quantitative information of CAP residues was acquired effectively by EESI-Ion Trap MS n . Using heated methanol-N 2 as spray reagent, we reduced the limit of determination (LOD) from 73.3 ng/mL to 0.3 ng/mL, and the CAP detection is linear in the range of 1–5000 ng/mL ( R = 0.9947). For the honey samples with CAP of 10, 100, and 1000 ng/mL, the recoveries were 133.0, 80.6, and 101.1 %, and the relative standard deviations were 5.96, 8.82, and 8.71 %, respectively. The reproducibility assays showed the stability of this method. Therefore, this ND-EESI-MS method is powerful for direct, rapid, and quantitative CAP analysis in honey samples with high sensitivity, precision, and specificity.
In the current neutral desorption-extractive electrospray ionization mass spectrometry (ND-EESI-MS) method, N 2 is inlet into samples to desorb chloramphenicol (CAP). We tried to use some organic solvents as the spray reagent to dissolve CAP, and then the best neutral desorption efficiency was obtained when using methanol. We applied this modified ND-EESI-MS method to detect CAP in honey samples only with sample dilution. The limit of CAP detection was then reduced from 73.3 to 0.3 ng/mL, reaching the current EU standard. Therefore, this is a powerful method for direct, rapid, and quantitative CAP analysis in honey samples.
研究领域:
分析化学
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Manman Qin,Leting Wang,Kaixin Xu,Zhehao Liang,Chao Zhong,Yanfei Xie,Dan Shan,Jun Yu,Jiang Wang,Hao Fan
High-viscosity rice soup is not only a popular food item but also a traditional Chinese medicinal remedy. Herein, we developed a high-throughput characterization platform for high-viscosity rice soup analysis using Neutral Desorption Extractive Electrospray Ionization Mass Spectrometry (ND-EESI-MS) with untargeted metabolomics. The chemical fingerprint of high-viscosity rice soup was directly analyzed by ND-EESI-MS, with exogenous (e.g., heavy metals and pesticides) and endogenous pollutants detected via qualitative and quantitative tandem mass spectrometry. Multivariate statistical analysis was then applied to distinguish the quality of the high-viscosity rice soup effectively. The concentrations of heavy metals and pesticide residues in high-viscosity rice soup were quantified, demonstrating a strong linear relationship (R2 > 0.99) across the 0.05–2000.00 ng mL−1 concentration range. The limits of detection (LOD) ranged from 0.27 ng mL−1 to 2.18 ng mL−1, while the limits of quantification (LOQ) ranged from 0.82 ng mL−1 to 6.61 ng mL−1. Furthermore, three specific compounds—3-methyl-2-butenoic acid, salicylic acid, and rhamnose—were quantified. These compounds demonstrated excellent linearity (R2 > 0.99) within the linear range of 1.10–1500.00 ng mL−1. The LOD ranged from 0.56 to 1.30 ng mL−1, and the LOQ ranged from 1.71 to 3.95 ng mL−1. Owing to its capability for simultaneous detection of exogenous and endogenous contaminants in complex viscous matrices, this ND-EESI-MS platform demonstrated significant potential to enhance quality control in food safety surveillance and pharmaceutical manufacturing.
研究领域:
食品科学
·
分析化学
·
质谱学
·
代谢组学
·
质量控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2020
·
IF 3.721
Ahui Xue,Wenjing Liang,Shide Wen,Yuanyuan Gao,Xueyong Huang,Yuzhen Tong,Yingbin Hao,Liping Luo
Broccoli sprouts are rich in secondary metabolites, especially glucosinolates (GLs). The synthesis of GLs can be modified by light. In this study, extractive electrospray ionization-mass spectrometry (EESI-MS) was used to analyze the broccoli sprouts extracts (BSE) under different light quality treatments. Multivariable statistical methods were used to analyze the difference of different light quality treatments. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze the gene expression of key enzymes in the aliphatic-GLs biosynthesis pathway. Nineteen compounds, including 7 GLs, 3 phenolic acids, 6 amino acids and 3 organic acids were simultaneously detected in one minute by EESI-MS. Relative expression of MYB28 , BCAT4 , CYP79F1 , CYP83A1 and AOP3 were mostly upregulated in R5B5 (light ratios of red/blue 5:5) treatment. These findings suggest that blue light improving the accumulation of secondary metabolites in broccoli sprouts, and the R5B5 treatment was most beneficial. Furthermore, we established EESI-MS method to simultaneous and rapid detection of secondary metabolites in broccoli sprouts, especially GLs. This study could provide a reference for selecting proper LED lighting conditions to produce broccoli sprouts with high content of bioactive components, and provides light regulation technology and theoretical basis for the industrialized production of sprouts.
研究领域:
植物学
·
代谢组学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Youhuan Gong,Dong Wu,Xiaojie Yan,Qian Zhang,Wenfu Zheng,Bin Li,Huanwen Chen,Le Wang
The threat of drug-resistant bacteria is challenging, and it is urgent to explore new antibiotics. Gold nanoparticles (AuNPs) are known to be a group of promising antibacterial agents for replacing conventional antibiotics. Nevertheless, their antibacterial mechanism remains to be elucidated. Herein, we directly observed the interaction between antibacterial AuNPs and bacteria at the molecular level using neutral desorption extractive electrospray ionization mass spectrometry (ND-EESI-MS). We monitored and analyzed the dynamic changes of bacterial metabolites in real time after AuNP treatment. Ten substances representing 3 major metabolic pathways, including protein and nucleic acid synthesis, energy metabolism, and quorum sensing, were identified, indicating that AuNPs may exert antibacterial effects through multiple mechanisms influencing bacterial metabolism and communication. This study deepens the understanding of the antibacterial mechanism of AuNPs and is insightful for designing and screening new antibacterial agents.
研究领域:
纳米技术
·
微生物学
·
分析化学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）