Analytical Methods
·
2020
·Vol. 12
·期 22
·2872-2880
·
IF 2.596
Yahui Wang,Meng Yan,Qianqian Ji,Manman Wang,Qian Wang,Xuesheng Wang,Yulan Hao
In this work, a magnetic material (Fe3O4-NH2@MIL-101) was successfully prepared, and the material was used as a sorbent for the magnetic solid-phase extraction (MSPE) of trace level monohydroxy polycyclic aromatic hydrocarbons (OH-PAHs) from urine samples for the first time. The target analytes were quantified by high performance liquid chromatography coupled with fluorescence detection (HPLC-FLD). The MSPE key factors, which include the amount of adsorbent, extraction time, pH, the effect of salt, eluting solutions and eluant volume, were systematically optimized. Under the optimized conditions, the developed method showed good linearity (0.03–200 ng mL−1), low limits of detection (0.016–0.042 ng mL−1, signal-to-noise ratio = 3) and satisfactory repeatability (relative standard deviation ≤ 10.1%, n = 5). The method showed stable average recoveries ranging from 78.3% to 112.9% and the enrichment factors were 9 to 15. Besides the satisfactory method parameters, the total MPSE process could be completed in no more than 5 minutes. These results indicated that Fe3O4-NH2@MOF based MSPE was a simple, efficient and fast method which was suitable for MSPE of OH-PAHs from urine samples.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Separations
·
2021
·Vol. 8
·期 11
·196
·
IF 2.777
Qing Han,Xiaoxiao Wu,Yi Cao,Hua Zhang,Yuqin Zhao,Xuejun Kang,Huaiyuan Zhu
A novel magnetic solid phase extraction based on mercaptophenylboronic acid (MPBA)-functionalized Fe3O4-NH2@Au nanomaterial (Fe3O4-NH2@Au-MPBA) was developed for selective separation and enrichment of catecholamines (including dopamine, norepinephrine and adrenaline). Fe3O4-NH2@Au-MPBA nanoparticles were achieved by self-assembly-anchoring MPBA molecules on the surface of Fe3O4-NH2@Au nanocomposites, which were synthesized via a facial ultrasonic auxiliary in situ reduction process. The interaction between cis-diol from catecholamines and boronic acid was reversible and could be flexibly controlled by adjusting pH value. The catecholamines could be quickly adsorbed by Fe3O4-NH2@Au-MPBA in weak alkaline solution (pH 8.0–9.0) and subsequently released in acid solution (pH 1.0–2.0). The process of adsorption and dissociation was very fast. Furthermore, the three catecholamines could be detected in urine from children by high performance liquid chromatography (HPLC) with electrochemical detector. Under optimal conditions, norepinephrine (NE), epinephrine (EP) and dopamine (DA) were separated very well from internal standard and exhibited a good linearity in the range of 2.5–500.0 ng mL−1, with correlation coefficients of r2> 0.9907. Limits of detection (LOD) (signal to noise = 3) were 0.39, 0.27 and 0.60 ng mL−1for NE, EP and DA, respectively. Recoveries for the spiked catecholamines were in the range of 85.4–105.2% with the relative standard deviation (RSD) < 11.5%.Keywords:Fe3O4-NH2@Au;magnetic solid phase extraction;catecholamines;HPLC;electrochemical detection;urine
研究领域:
分析化学
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纳米技术
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生物化学
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产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 2.892
Juan Han,Li Wang,Yun Wang,Jian Dong,Xu Tang,Liang Ni,Lei Wang
A geometrically symmetric four – arm dendritic polymer 4-arm-PEG-NH 2 was employed to modify on the surface of Fe 3 O 4 as support for cellulase immobilization. Glutaraldehyde was used as a coupling agent of this magnetic support to covalently immobilize cellulase. The morphology and properties of the synthesized magnetic material were characterized by Fourier transform infrared (FT-IR) spectroscopy, vibrating-sample magnetometer (VSM), thermogravimetric analysis (TGA), transmission electron microscopy (TEM) and X-ray diffraction (XRD). The properties of immobilized enzyme such as the optimum temperature and pH, Michaelis constant, thermal stability, reusability and storage stability were investigated. The loading capacity of cellulase was 132 mg/g. Compared with free cellulase, immobilized cellulase showed wider pH and temperature ranges, higher operational stability and good storage stability. Besides, the results of hydrolysis filter paper and microcrystalline cellulose showed that the catalytic effect of immobilized cellulase on the free cellulase was increased by about 76%.
研究领域:
生物化学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
·
IF 13.6
Man-Man Wang,Zi-Ling Li,Han Wu,Ke-Yan Chen,Fan Guo,Gui-Fu Zuo,Yu He,Xue-Bo Yin
Magnetic solid-phase extraction (MSPE) was developed based on a well-designed Fe 3 O 4 -NH 2 @g-C 3 N 4 nanocomposite as sorbent for a mixture of six benzophenones (BPs) in environmental water samples. The composite fabricated via in-situ self-assembled g-C 3 N 4 shell with homogeneous polymerization of cyanuric chloride and cyanuric acid on Fe 3 O 4 -NH 2 core. While high adsorption capacity was derived from g-C 3 N 4 via hydrophobic, π-π and hydrogen bonding interactions to the targets, the fast magnetic separation was realized with Fe 3 O 4 core for less solvent consumption. In combination with LC-MS/MS, the Fe 3 O 4 -NH 2 @g-C 3 N 4 sorbent minimized the interfering components, reduced the matrix effects, and provided the enrichment factors of 121–150 for six BPs with relative standard deviations ≤ 9.7% even after 20 times extraction-desorption cycles. The present method gave the detection limits of 0.3–2.5 ng/L for six BPs with the linear ranges of 1.0–2000 ng/L, and the recoveries of 84.6%−104% in sea water and 86.2%−107% in lake water samples. Thus, the Fe 3 O 4 -NH 2 @g-C 3 N 4 -based MSPE coupled with LC-MS/MS method provided a convenient, efficient, and reliable alternative to monitor trace BPs in environmental water samples.
研究领域:
环境科学
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Tiemei Li,Xin Zhang,Lichong Duan,Shuhao Cui,Yingyu Chang,Xia Zhang
A new ferrofluid based on hydrophobic deep eutectic solvent and Fe 3 O 4 @NH 2 -graphene oxide was designed, and then used as extraction medium for the extraction and analysis of estrogens in water by coupled with vortex-assisted liquid-liquid micoextraction and high performance liquid chromatography. The morphology, structure, thermal stability and magnetic property of the material were studied by scanning electron microscope (SEM), Fourier Transform-Infrared Spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and vibrating sample magnetometer (VSM). Four estrogens, including estradiol (E2), ethinyl estradiol (EE2), bisphenol AP (BPAP) and hexestrol (HEX), were used as the model analytes. Key parameters related to the ferrofluid preparation and extraction conditions were optimized by single-factor and response surface method. Under the optimal conditions, good linearity (R 2 > 0.99) in the concentration ranges of 0.1–200, 0.2–200, 0.007–200 and 0.15–200 μg/L were obtained for E2, EE2, BPAP and HEX, respectively. The limits of detection for E2, EE2, BPAP and HEX were 0.030, 0.070, 0.002 and 0.05 μg/L, respectively. The established method was successfully applied for the quantification of E2, EE2, BPAP and HEX in urine, environmental water, beverage and package samples. The method was simple, rapid, sensitive and producible.
研究领域:
分析化学
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纳米技术
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环境科学
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材料科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
NANO
·
2024
·
IF 1
Ying Zhou,Jinai Bian,Min Tian,Tingting Wang,Guomei Zhang,Yan Zhang,Caihong Zhang
Chronic ingestion of food containing clenbuterol (CLB) in the human body can cause acute poisoning. Thus, it is essential to develop a sensitive CLB detection way. Here, a novel electrochemical aptamer sensor based on aptamer/gold nanoparticles/amino magnetic ferric oxide/graphene nanosheets (aptamer/Au NPs/Fe 3 O 4 –NH 2 /GN) nanocomposite has been developed. The Fe 3 O 4 –NH 2 nanoparticles were self-assembled on GN, and then Fe 3 O 4 –NH 2 /GN nanocomposite was coated on the glassy carbon electrode (GCE) surface. Afterward, Au NPs were electrodeposited on Fe 3 O 4 –NH 2 /GN nanocomposite to fix the aptamer by the Au–S bonds. When CLB was added to the solution, the aptamer will specifically recognize CLB, causing current changes. Under the optimized conditions, the sensor shows better electrochemical performance (linear range is 1 × 1 0 − 1 3 M to 1 × 1 0 − 7 M, the detection limit is 1 . 1 6 × 1 0 − 1 4 M ( S / N = 3 )). It has been successfully applied to the detection of CLB in actual samples. The results show that the aptamer sensor has good selectivity, stability and repeatability, which will provide a novel idea for more sensitive and quicker CLB detection.
研究领域:
分析化学
·
生物传感器
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食品安全
·
纳米材料
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·7448
·
IF 4.259
Han Lina,Li Qinglian,Chen Shuai,Xie Wei,Bao Weiren,Chang Liping,Wang Jiancheng
A sort of magnetical material named Fe 3 O 4 –NH 2 –Pd was prepared by loading varying amounts of immobilizing Pd on the surface of the magnetic Fe 3 O 4 –NH 2 microspheres. This magnetical material was used firstly for capturing Hg° from coal derived fuel gas based on its recoverability. The experimental results showed that the loading Pd on the amine-functionalized magnetite nanoparticles can greatly improve the efficiency of removing Hg° at a high temperature range between 200 and 300 °C. The magnetic Fe 3 O 4 –NH 2 –Pd sorbent with 5% Pd loaded exhibited significantly high activity and stability in capturing Hg°, affording over 93% capture efficiency at 200 °C for more than 8 hrs. Compared to the Fe 3 O 4 –NH 2 sorbent that converted the Hg° as HgS, this Fe 3 O 4 –NH 2 –Pd sorbent can remove the Hg° by forming Pd-Hg amalgam and HgS. In addition, the experimental tests indicated that the as-synthesized Fe 3 O 4 –NH 2 –Pd sorbent still showed stable magnetic properties after two regeneration cycles in removing Hg°, which provided the opportunity for preparing a recyclable sorbent which can be easily separated and recovered for Hg° removal.
研究领域:
环境科学
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材料化学
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能源工程
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污染控制
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Liuying He,Xiao Wang,Junyu Chen,Yuze Li,Liping Wang,Caiqiao Xiong,Zongxiu Nie
Liver cancer (LC) is the third frequent cause of death worldwide, so early diagnosis of liver cancer patients is crucial for disease management. Herein, we applied NH2-coated polystyrene@Fe3O4 magnetic beads (PS@Fe3O4–NH2 MBs) as a matrix material in laser desorption/ionization mass spectrometry (LDI-MS). Rapid, sensitive, and selective metabolic profiling of the native biofluids was achieved without any inconvenient enrichment or purification. Then, based on the selected m/z features, LC patients were discriminated from healthy controls (HCs) by machine learning, with the high area under the curve (AUC) values for urine and serum assessments (0.962 and 0.935). Moreover, initial-diagnosed and subsequent-visited LC patients were also differentiated, which indicates potential applications of this method in early diagnosis. Furthermore, among these identified compounds by FT-ICR MS, the expression level of some metabolites changed from HCs to LCs, including 29 and 12 characteristic metabolites in human urine and serum samples, respectively. These results suggest that PS@Fe3O4–NH2 MBs-assisted LDI-MS coupled with machine learning is feasible for LC clinical diagnosis.
研究领域:
医学
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分析化学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Qingying Wang,Lianfang Chen,Xiaoyan Cui,Jie Zhang,Yaohui Wang,Xiupei Yang
Herein, a covalent organic framework (COF) was grown on a magnetic metal-organic framework (MOF) by a solvothermal method for the efficient extraction of bisphenols (BPs). The magnetic solid-phase extraction (MSPE) of four bisphenols (bisphenol A, bisphenol B , bisphenol AF and bisphenol C) was carried out without adjusting the pH and salt concentration. When 30 mg Fe 3 O 4 @NH 2 -MIL-88(Fe)@TpPa was used to adsorb for 25 min, 6 mL methanol was used to elute for 20 min, and the extract was detected by high-performance liquid chromatography (HPLC). The proposed method has a low detection limit of 0.011–0.036 ng mL −1 , a wide linear range of 0.05–100 ng mL −1 , and a correlation coefficient (R 2 ) of 0.9980–0.9998. The intra-day and inter-day precisions are 0.74–2.54% and 1.68–3.72%, respectively. Bisphenol A was determined by applying the proposed method to the determination of actual milk samples. The standard addition experiment showed that the relative recovery of the four bisphenols was 85.70–119.7%. Pseudosecond-order, first-order, Langmuir and Freundlich models were applied to explore the adsorption characteristics of Fe 3 O 4 @NH 2 -MIL-88(Fe)@TpPa. In general, the established Fe 3 O 4 @NH 2 -MIL-88(Fe)@TpPa-MSPE-HPLC-UV method exhibits attractive sensitivity, simple manipulation, and excellent reusability, and it has excellent prospects for the detection of trace BPs in complex milk matrices.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Nengjing Yang,Hongbin Pu,Da-Wen Sun
The unreasonable use of organophosphorus pesticides leads to excessive pesticide residues in food, seriously threatening public health, and the potential of surface-enhanced Raman spectroscopy (SERS) technology, incorporating a metal–organic framework, is substantial for the rapid detection of trace pesticide residues. Here, a novel Fe 3 O 4 @NH 2 -MIL-101(Fe)@Ag (FNMA) SERS nanosensor was developed. Results indicated that the FNMA had a high enhancement factor of 1.53 × 10 8 , a low limit of detection (LOD) of 4.55 × 10 −12 M, and a relative standard deviation of 7.73 % for 4-nitrothiophenol, demonstrating its good SERS sensitivity and uniformity, and also possessed good storage stability for one month. In quantifying fenthion and methyl parathion in standard solutions and apple juice in the range of 0.05/0.02–20 mg/L, it showed LODs of 3.02 × 10 −3 mg/L and 1.43 × 10 −3 mg/L, and 0.0407 and 0.0075 mg/L, respectively, demonstrating potentials in ultrasensitive trace detection of pesticides in food.
研究领域:
分析化学
·
食品安全
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Enhui Wu,Yuexian Li,Qing Huang,Zhenkai Yang,Anyu Wei,Qi Hu
An amino-functionalized magnetic metal organic framework (MOF), Fe 3 O 4 -NH 2 @MIL-101(Cr), was employed for laccase immobilization for the first time. The immobilized laccase was synthesized by the adsorption and covalent binding method, thus exhibited high activity recovery, large immobilization capacity and good tolerance to low pH and high temperature conditions. The excellent stability enabled the immobilized laccase to retain 89% of its initial activity after storage for 28 days. When the ambient temperature reached 85 °C, the immobilized laccase showed 49.1% residual activity even after 6 h preservation. The stability of laccase in organic solvents such as methanol was also greatly improved. Application of the immobilized laccase for 2,4-dichlorophenol removal was also investigated. The adsorption by Fe 3 O 4 -NH 2 @MIL-101(Cr) contributed to a quick removal in the first hour, and the removal efficiency reached 87% eventually. When the reaction was completed, the immobilized laccase could be separated from the solution by a magnet. The results introduced a novel support for laccase immobilization, and the immobilized laccase had great potential in wastewater treatment .
研究领域:
环境科学
·
生物技术
·
废水处理
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 32
·12563-12569
·
IF 3.069
Huixiao Duo,Xiaofeng Lu,Shuai Wang,Licheng Wang,Yong Guo,Xiaojing Liang
Herein, a novel, fusiform-like magnetic metal–organic framework material (Fe3O4-NH2@MOF-235) was fabricated by a facile two-step solvothermal approach. Then, Fe3O4-NH2@MOF-235 was applied as an adsorbent in magnetic solid-phase extraction (MSPE) coupled with high-performance liquid chromatography (HPLC) for the simultaneous extraction and determination of five kinds of benzoylurea insecticides (flufenoxuron, chlorbenzuron, teflubenzuron, diflubenzuron and triflumuron) from honey, fruit juice and tap water samples. Moreover, potential variables influencing the MSPE efficiency were optimized. Under the optimized conditions, an excellent linearity was achieved in the range of 1.0–300.0 μg L−1 for teflubenzuron and flufenoxuron and 2.0–300.0 μg L−1 for diflubenzuron, chlorbenzuron and triflumuron, with the correlation coefficients of 0.9991–0.9995. The limits of detection for the analytes were 0.25–0.5 μg L−1, and the recoveries at three fortified concentration levels ranged from 76.45% to 90.74% for the honey samples, 79.95–95.27% for the fruit juice samples, and 84.02–99.62% for the tap water samples. The π–π stacking and hydrophobic interactions between Fe3O4-NH2@MOF-235 and the analytes played an important role in the adsorption process. Moreover, maximum adsorption could be achieved within 5 minutes with very little adsorbent for all the samples. Therefore, the magnetic Fe3O4-NH2@MOF-235 composite combined with MSPE is a convenient approach to detect benzoylurea insecticides in actual samples.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）