JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2020
·
IF 3.721
Qing Shen,Gongshuai Song,Honghai Wang,Yanping Zhang,Yiwei Cui,Hujun Xie,Jing Xue,Haixing Wang
Shrimp waste contains a large number of bioactive lipids for preparing functional foods. In this study, a 1,2-dichloroethane based method for extracting phospholipids from shrimp waste was developed. The number of phospholipids recovered by 1,2-dichloroethane/methanol at a ratio of 1:2 (v/v) was 10.97 g/100 g, which was comparable with the performance of conventional methods. The lipidomics profile of the extract from shrimp waste was studied through gas chromatography with flame ionization detection (GC/FID) and hydrophilic interaction liquid chromatography coupled to QTrap mass spectrometry (HILIC-QTrap/MS) to reveal the effect of different extraction methods on the selectivity of lipids. It was found that the lipid extracts using different methods showed significant variation, while the diversity and percentage of each molecular species were generally the same. Besides, a large number of health beneficial dietary ω-3 polyunsaturated fatty acid (PUFA) structured phospholipids were detected, which indicated that shrimp waste was an important source with significant nutritional values.
研究领域:
食品科学
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生物化学
·
营养学
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DOI
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2025
·
IF 3.7
Jian Zou,Gang Wang,Shuyu Duan,Chenyi Shi,Xiaobing Yun,Xiuhan Yang,Zhe Du,Huan Qiu,Winniford Bill
An isostatic aroma permeation measurement system was developed using a customized permeation cup and dynamic SPME-GC-FID coupled to a flow-cell unit. The limit of quantitation (LOQ) of the method ranged from 0.012 to 0.054 mg/m 3 with a PDMS-coated SPME Arrow, with typical linearity from 5 to 600 mg/m 3 . The system's repeatability on a polyethylene terephthalate (PET) film was between 8.7% and 25.0% (%RSD, n = 4). The results were verified using headspace GC–MS and thermal desorption GC–MS, with relative average deviation (%RAD) ranging from 6.0% to 20.7%. The aroma barrier performance of six film samples, including PET, biaxially oriented polypropylene (BOPP) and vacuum metalized polyethylene (PET//VMPE), was compared using this method with a mixture of five model aroma compounds, such as 2-methyl pyrazine, 2-heptanone and benzaldehyde. This method provides a sensitive, automated and low-cost solution for evaluating the aroma barrier performance of polymer films, with potential applications in the packaging industry.
研究领域:
分析化学
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食品科学与工程
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DOI
高影响因子
Food Quality and Safety
·
2023
·
IF 5.6
Li Yang,Wu Minyu,Zhai Liang,Zhang Hui,Shen Lirong
Camellia oil and olive oil with superior economic value are easily adulterated with other, cheaper oils. It is difficult to identify both oils by traditional methods because of their similar fatty acid profiles. In the present study, a novel method for qualitative and quantitative analysis of β-sitosterol using GC/MS and GC/FID was developed to identify camellia oil and olive oil. The method validation of β-sitosterol analysis showed good linearity and satisfactory values for recovery, accuracy, precision, and repeatability. The linear regression coefficient (R2) of the calibration curve was 0.9985. An acceptable limit of detection (0.36 mg/100 g) and limit of quantification (1.20 mg/100 g) were achieved. The spiked recoveries were 95.0% to 100.3%. The relative standard deviation (RSD) of within-day precision was less than 3.26%, and the RSD of retention times and peak areas for repeatability were within 0.03% and 1.08%, respectively. The contents of β-sitosterol in virgin camellia oil and virgin olive oil were in the range of 14.1–30.2 mg/100 g and 94.3–173.2 mg/100 g, respectively, indicating that the β-sitosterol content in the former is seven times that in the latter, and β-sitosterol could be a potential marker for the authentication and adulteration detection of both oils.
研究领域:
食品科学
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分析化学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2020
·
IF 4.049
Wenli Zhu,Jing Zhang,Xiaowan Zhang,Lizhen Han,Peige Qin,Shufang Tian,Qian Zhou,Xuebin Zhang,Minghua Lu
The Al-doped mesoporous crystalline material-41 (Al-MCM-41) composite was prepared and applied as fiber coating material of headspace solid-phase microextraction (HS-SPME) for extraction of polycyclic aromatic hydrocarbons (PAHs) from human urine. Five PAHs including acenaphthene, fluorene, phenanthrene, anthracene, and pyrene are chosen as target analytes to evaluate the performance of the material by GC-FID analysis. The mesoporous Al-MCM-41 composite was characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, nitrogen adsorption/desorption measurement, and thermogravimetric analysis. The parameters affecting the extraction efficiency of HS-SPME were investigated. Under the optimal conditions, the method exhibits ideal linearity for target analytes in the range of 0.3–600 ng⋅mL -1 with the coefficients (R 2 ) equal or higher than 0.9906. The enrichment factors are calculated from 540 to 1760. The limits of detection (LODs) and limits of quantitation (LOQs) are between the ranges of 0.06–0.18 and 0.3–0.9 ng⋅mL -1 , respectively. The relative standard deviations (RSDs) ( n = 5) of intra-day and inter-day are in the ranges of 1.08–7.49% and 2.84–18.3% respectively. The fiber-to-fiber reproducibility ( n = 3) is in the range of 6.47–13.9%. The method was successfully applied for the analysis of PAHs in human urine with reasonable recoveries which is ranging from 73.29 to 116.1%.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
Analytical Methods
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2017
·Vol. 9
·期 35
·5101-5107
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IF 1.9
Rongwei Yan,Leilei Zhao,Yanfei Li,Yong Zou,Xinjun Xu
As a novel injection preparation, propofol liposomes have extensive application prospects in clinical practice, and lipid components in them need to be quantified for quality control. In this study, a simple and accurate GC-FID method coupled with a methyl esterification pretreatment was developed for simultaneous determination of three main excipients (tricaprylin, TA; dipalmitoyl phosphatidylglycerol, DPPG; 2-dierucoyl-sn-glycero-3-phosphocholine, DEPC) in propofol liposomes with stearic acid used as the internal standard (IS). Response surface methodology was utilized for optimizing three factors of the methyl esterification reaction (the temperature, BF3 concentration and reaction time were optimized to be 75.0 °C, 14.5% and 6.5 min, respectively). Under optimum conditions, the average methyl esterification rate of the three analytes reached 96.43 ± 0.16% (n = 3). Moreover, multiple sample solutions could be simultaneously prepared, which would promote the efficiency and accuracy of quantitative analysis. Three calibration curves had good linear regression (r > 0.9999) within the tested ranges, the average recoveries ranged from 98.64% to 102.44% and the RSDs of the precision, repeatability, stability and recoveries were all below 1.53%. Finally, the validated method was successfully applied to the quantitative analysis of the three analytes in six batches of propofol liposomes.
研究领域:
药学
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分析化学
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DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 18
·2443-2449
·
IF 2.378
Xu Liang,Jian-Hai Wu,Qi-Yue Zhao,Xiu-Ping Dong,Liang Dong,Lei Qin,Xian-Bing Xu,Ming Du
The total tetramethylpyrazine (TMP) content in vinegar samples could be detected by multiple HS-SPME coupled with GC-FID (MHS-SPME-GC-FID) as a routine method. Unlike the traditional HS-SPME method, only one working curve built with different levels of standard aqueous solutions in the MHS-SPME-GC-FID method was able to calibrate the total TMP level from different matrixes (calibration standards ranging from 0.05 to 5.00 μg mL−1, R2 = 0.9986). In addition, the developed method was sensitive to the determination of TMP in vinegars (the limit of detection of 1 μg L−1, the limit of quantification of 5 μg L−1). In addition, the excellent recovery at 0.10 μg mL−1, 1.00 μg mL−1 and 5.00 μg mL−1 of TMP (ranging from 81.01% to 113.5%) and good reproducibility of the method (inter-day RSD 7.79%, intra-day RSD 3.94%) confirmed that the developed method was accurate and stable for the analysis of vinegar samples. Moreover, the detection results in real samples were consistent with the results from the traditional HS-SPME-GC-FID corrected by external standard method. In conclusion, the uniqueness of the working curve and the absence of matrix effect in MHS-SPME-GC-FID method were useful for rapidly obtaining comparable data from liquid samples with different matrix.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2025
·
IF 4
Lang Liu,Jianyi Zhang,Yanqin Zhao,Xunan Ning
Nonylphenols (NPs) are persistent endocrine-disrupting organic pollutant. Most studies focus on total NP content, with limited research on individual isomers. This study developed a method to separation and detection of 10 NP isomers using GC-FID-MS combined with Deans switch. It can effectively transfer NPs from the first gas chromatography column (DB-5MS) to the second column (CP-ChiraSil-DEXCB), achieving efficient separation of the 10 isomers. Sewage and sludge samples from wastewater treatment plants were prepared using solid-phase extraction (SPE) and solid-liquid extraction. NP isomers in sewage samples were enriched with a phenol-specific SPE column, rinsed with 10 mL methanol-water (v/v 2:8), and eluted with 3 mL methanol and 5 mL dichloromethane. NP isomers in sludge samples underwent three ultrasonic extractions with 30 mL ethyl acetate-dichloromethane (v/v 1:1). Concentrate the extract to 1 mL and then purified using an LC NH2 column, rinsed with 9 mL of ethyl acetate-n-hexane (v/v 1:9), and eluted with 10 mL of ethyl acetate-dichloromethane (v/v 1:1) .The results showed that the recovery rates for NP isomers ranged from 70.82 % to 110.12 % in sewage (LOQ: 0.09–0.31 μg/L; RSD: 1.82 %-10.12 %) and from 72.82 % to 114.12 % in sludge (LOQ: 0.15–0.47 μg/kg; RSD: 0.23 %-11.28 %), complying with the USEPA standards (70 %-130 %). Application of the method to real samples detected all 10 NP isomers, with concentrations of 0.50–5.01 μg/L in sewage and 0.13–24.91 μg/g in sludge.This method provides a reliable approach for the detection of NP isomers in complex environmental matrices.
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2013
·Vol. 76
·期 11
·671-678
·
IF 1.437
Cui Shumin,Chen Qianxia,Wang Weiping,Miao Jigen,Wang Aijun,Chen Jianrong
In this study, a simple and efficient method has been developed to analyze pesticides in water samples using ultrasonic-assisted dispersive liquid–liquid microextraction (UA-DLLME) combined with gas chromatography-flame ionization detection (GC-FID). Several parameters, including type and volume of extractant and dispersant, extraction time, and amount of salt on extraction performance, were optimized in detail. A mixture of acetonitrile (1.0 mL, dispersant) and carbon tetrachloride (15 μL, extractant) was used for extraction. Under optimal conditions, enrichment factors were obtained between 315 and 1153. The linearity of the method ranged from 1 to 100 μg L −1 with correlation coefficients ≥0.9990. Limits of detection (S/N = 3) ranged between 0.09 and 0.57 μg L −1 , depending on the compounds. Relative standard deviations were <8.0 % ( n = 5) for both intra- and inter-day analyses. The proposed method was successfully applied for the preconcentration and determination of pesticides in water samples (river water, tap water, and lake water) with recoveries that varied from 90.5 to 107.7 %.
研究领域:
分析化学
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环境科学
·
水质分析
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2026
·
IF 5.1
Qing-nan Chen,Yun-e Bai,Yao Ma,Rui Liao,Lu-lu Wang,Shuang Hu,Jian- ping Gao
Latex-bearing plants have gained global attention, but few studies have been reported on Codonopsis pilosula (Franch.) Nannf.( C. pilosula ) latex. In this research, we established a UHPLC-MS/MS method to investigate the constituents in C. pilosula latex. Analysis revealed that the latex of C. pilosula contains alkaloids, terpenoids, and fatty acids, with fatty acids being one of the primary metabolites distinguishing the latex from above-ground (DS) and below-ground (DX) parts of C. pilosula. To evaluate the quality of C. pilosula latex, an approach of dispersive liquid-liquid microextraction with solidification of the floating organic drop (DLLME-SFO) methodology was developed to quantitatively analyze the contents of three fatty acids (palmitic, linoleic, and stearic) in the latex of C. pilosula . The ideal extraction conditions involve injecting 20 μL of dodecanol extractant into a 5 mL 10% NaCl solution at pH 3.0, followed by 20 min at 700 rpm. The three components mentioned above have enrichment coefficients ranging from 48 to 50. The quantification limits were between 0.23 and 0.25 μg/mL, and three components demonstrated good linearity in their respective linear ranges (R 2 > 0.995). The satisfied precision and recovery rates were 1.26 to 8.05% and 92.38 to 101.53%, respectively. By quantitatively analyzing the three fatty acid contents in the latex of C. pilosula , the results showed that palmitic and linoleic acids were able to successfully distinguish the latex of C. pilosula from different parts, which laid the foundation for the quality evaluation of C. pilosula , and provided a reference for the utilization of C. pilosula , a medicinal food source.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2026
·
IF 4
Chenqing Zhang,Juan Zhang,Zhiyu Zhang,Jing Yan,Zhuoyue Hou,Shusheng Zhang,Kai Hu
Short-chain fatty acids (SCFAs) are key biomarkers of gut microbiota activity and are implicated in various metabolic, inflammatory, and neurological diseases. This study fabricated a phenothiazine-based hyper-crosslinked polymer (PTZ-HCP) and applied it as a solid-phase microextraction (SPME) fiber coating for the analysis of nine SCFAs in rat feces by gas chromatography with flame ionization detection (GC-FID). The prepared PTZ-HCP was characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, N 2 adsorption-desorption measurements, and thermogravimetric analysis. These analyses confirmed its successful synthesis and revealed a porous structure, high specific surface area, and excellent thermal stability. Owing to these structural advantages and the capability for multiple functional groups, the PTZ-HCP coatings demonstrated outstanding extraction efficiency and high adsorption capacity for SCFAs. An SPME/GC-FID method using PTZ-HCP coated fibers were established and validated. The validation results demonstrated significant advantages, including a wide linear range (1.0 × 10 -3 -0.50 μg·mL -1 for 2-methylvaleric acid; 1.0 × 10 -3 -2.0 μg·mL -1 for 4-methylvaleric acid), low LODs (3.0 × 10 -4 μg·mL -1 for 2-methylvaleric acid and 4-methylvaleric acid), and good precision (≤ 9.5%). This work presents a novel SPME coating material that effectively addresses key challenges in the extraction and analysis of SCFAs in rat fecal samples. Moreover, it verifies that Coptis chinensis modulates gut microbiota by altering SCFA levels, thereby offering important insights for research on the pathological mechanisms and treatment of Parkinson’s disease.
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PubMed
高影响因子
Food Analytical Methods
·
2020
·Vol. 13
·期 10
·1938-1946
·
IF 2.667
Pang Yue-Hong,Sun Miao,Shen Xiao-Fang,Yue Qi,Ma Tian-Tian,Yang Cheng
Benzo[a]pyrene (BaP) is a kind of trace pollutant commonly found in environment, food, and organism. It has been widely concerned because of its strong carcinogenicity to the human body. In this paper, covalent organic framework TpBD, in situ growth on titanium wire, was used as the coating of solid-phase microextraction (SPME) fiber and coupled with gas chromatography–flame ionization detector (GC-FID) for the detection of trace BaP in roast meat samples. The linear range of the developed method under the optimized experimental conditions was 0.02–15 μg L −1 , the detection limit (S/ N = 3) was 0.006 μg L −1 , and the limit of quantification (S/ N = 10) was 0.02 μg L −1 . The intra-day and inter-day precision of a single fiber were 1.73% and 2.27%, respectively. The relative standard deviation (RSD) between fiber-to-fiber was investigated by three as-fabricated fibers was 6.09%. Moreover, the enrichment factor of the fabricated TpBD SPME fiber for BaP was 4914, and the fiber can withstand at least 150 cycles of adsorption and desorption without significantly decrease in extraction efficiency (< 3.6%). Finally, the developed method was successfully applied to the detection of BaP in roast meat samples with the recoveries from 95.4 to 108.5%.
研究领域:
分析化学
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食品安全
·
环境科学
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DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Tiemei Li,Yuefei Song,Jingjie Xu,Jing Fan
As a new type of green solvents , deep eutectic solvents (DESs) are attracting more and more attentions due to their unique properties and cheapness. However, most DESs reported previously were hydrophilic and thus limiting their applications in aqueous medium. In this study, a novel hydrophobic DES was prepared by mixing ethylparaben and methyl trioctyl ammonium chloride , and density, viscosity, and contact angle of the DES were determined to character its solvent characteristics. Then, this DES was used as an efficient additive of sol-gel sorbent coating of poly(dimethylsiloxane) (PDMS) fiber. It was found that the addition of hydrophobic DES could generate a lot of neat pores in the PDMS fiber surface, thus significantly improving the performance of PDMS fiber coating. The feasibility of PDMS-DES adopted for head space solid phase microextraction was evaluated by GC-FID for the extraction and determination of volatile organic compounds, such as toluene, ethylbenzene and o -xylene. Key parameters affecting the extraction efficiency were investigated systematically. Under optimal conditions, the linear range with PDMS-DES fiber lied between 10 and 1000 μg L −1 , and the limit of detection (LOD) was in the range from 0.005 to 0.025 μg L −1 . Compared with homemade PDMS fiber, the peak area of signal response for the PDMS-DES fiber was 3 times higher. At the same time, the LOD for the proposed method was much lower than the commercial 100 µm-PDMS fiber coating.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）