TECHNOLOGY AND HEALTH CARE
·
2016
·
IF 0.678
Zhang Yujuan,Song Na,Fu Jiamin,Liu Yanling,Yu Yanrong,Shi Qiaofa,Fu Yingyuan,Zhou Nanjing,Yuan Keng,Zhao Lin,Zhang Quan,Min Weiping
RNA interference (RNAi) employs double-stranded RNA or
siRNA (small interfering RNA) to silence gene expression in cells. The
widespread use of RNAi therapeutics requires the development of clinically
suitable, safe and effective delivery vehicles. PEI (Poly(ethylene imine))
carrying the positive charges has attracted considerable attention for siRNA
delivery. Gold nanorods (GNRs) exhibit specially localized surface plasmon
resonance when excited by the visible and near-infrared laser, which is
useful for photothermal therapy. However, the toxicity derived from a large
amount of the surfactant cetyltrimethylammonium bromide (CTAB) during GNR
synthesis severely limits their medical applications. Here, we report the
synthesis of GNRs-PEI/GNRs-PEI-folate to improve biocompatibility, siRNA
delivery and photothermal therapy of GNRs. Firstly, GNRs were synthesized
according to the seed-mediated template-assisted protocol. The
characterization results of GNRs showed: the size was length about 218 nm
and width about 26.8 nm; the Zeta potential was +38.1 mV derived from CTAB
on their surface; the dipole resonance extinction spectrum peak was 752 nm
which is effective for photothermal therapy in vivo. Secondly, we
synthesized PEI-MUA (Mercaptoundecanoic acid) and PEI-MUA-folate based on
the chemical reaction between amino group of PEI and carboxyl group of MUA
or Folate. PEI-MUA or PEI-MUA-folate to replace CTAB on GNRs obtained the
GNRs-MUA-PEI system or the GNRs-MUA-PEI-folate system due to the solid
conjugation between the thiol group of MUA and GNRs. The products were
measured using the FTIR Spectrometer, and the spectra suggest MUA-PEI or
PEI-MUA-folate has successfully replaced CTAB on the surface of GNRs.
Finally, GNRs-MUA-PEI was incubated with siRNA-Cy3. The unbound siRNA-Cy3
was measured the intensity of fluorescence for calculating the uploaded
amount of siRNA by GNRs-MUA-PEI, and the results indicate that the uploaded
percentage of siRNA is about 70%. We conclude that the GNRs-MUA-PEI
system is an effective siRNA loading vehicle.
研究领域:
纳米医学
·
基因治疗
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
OPTICAL FIBER TECHNOLOGY
·
2026
·
IF 2.7
Yumei Zhang,Baoquan Xiao,Zelin Gao,Li Yang,Dekui Zhang,Ning Wang,Tuanjie Che,Xusheng Xia,Liyun Ding
The rapid, accurate and highly sensitive detection of acetylcholine, which was one of the neurotransmitters related to a variety of neurodegenerative diseases has great significance for disease screening, treatment and prognosis. This paper proposed a GNRs/Ti 3 C 2 MXene enhanced optical fiber LSPR acetylcholine biosensor by depositing Ti 3 C 2 MXene nanosheets on the surface of a GNRs-based optical fiber sensing probe via simple electrostatic self-assembly. The refractive index sensitivity of the biosensor was improved due to the advantages of the large specific surface area, hydrophilicity and broadband absorption spectrum of 2D Ti 3 C 2 MXene nanosheets. The refractive index sensitivity of the biosensor with Ti 3 C 2 MXene nanosheets was improved by 44 % compared to that of the GNRs-based biosensor. The biosensor had a good linear relationship in the range of 0–900 μM for acetylcholine concentration with a sensitivity of 0.0304 nm/μM and the detection limit was 6.58 μM. The results indicated that the proposed GNRs/Ti 3 C 2 MXene enhanced optical fiber LSPR acetylcholine biosensor provides a promising avenue of detection for in biotechnology and medicine files.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2021
·
IF 5.875
Ying Fang,KangRui Wang,Qin Li,ChengHong Huang
Carboxylation chitosan achieved by alkalization linked with ethyl vanillin to obtain Chitosan-ethyl vanillin (EV-CMCS) compound through Schiff base reaction and confirmed by FT-IR, UV, XRD, TG and NMR. EV-CMCS refluxed with GNRs for acquisition of EV-CMCS@GNRs nanocomposites for PTX Loading and release. Results demonstrated that both EV-CMCS and EV-CMCS@GNRs are nanoscale composites with excellent solubilization due to their micelle structure taking CMC values of 0.06683 mg/mL and 0.06537 mg/mL. It was found that the loading and encapsulation rate of EV-CMCS and EV-CMCS@GNRs for PTX are 19.59~37.64% and 60.36~80.79% as well as 20.99~37.02% and 58.78~79.77%. Compared with only the delayed release of EV-CMCS that it have 11.5% and 18.7% accumulative release amount for 24 h and 14.9% and 23.7% for 48 h under both pH 6.8 and 7.4, the EV-CMCS@GNRs represent sudden release that it have an accumulative release amount of 90.2% for 24 h and 96.0% for 48 h at pH 6.8. It deduced that the broken Schiff base under acidic condition can increase CMC of EV-CMCS@GNRs, which offered an alternative way for paclitaxel delivery for tumor therapy.
研究领域:
纳米技术
·
药学
·
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Yumei Zhang,Liyun Ding,Shiyuan Wang,Xingdong Jiang,Fei Ma,Jue Zhao,Wenbo Meng,Long Gao
An enhanced resonance coupling of gold film-gold nanorods (GNRs) based optical fiber acetylcholine (Ach) biosensor was fabricated with increased sensitivity for refractive index (RI) sensing. Gold film with a thickness of about 50 nm is sputtered on the surface of the optical fiber by the magnetron sputtering, and then the GNRs synthesized by seed-mediated were immobilized on the surface of the gold film by using 1,6-hexodithiol as a crosslinker via cross-link method. The RI sensitivities (SRI) improvement rate of the sensors was 24.09% higher than that of the sensor without GNRs functionalization. Acetylcholinesterase (AchE), as the specific recognition substance of the biosensor, was immobilized on the surface of GNRs by the chemical crosslinking method to prepare optical fiber sensing probes. Under the optimum preparation conditions of the optical fiber sensing probe, the wavelength shift of the surface Plasmon resonance (SPR) peak has a good linear relationship with the concentration of Ach (CAch) in the range of 0.5– $5 \mu \text{g}$ /mL with the sensitivity of 0.4912 nm/( $\mu \text{g}$ /mL) and limit of detection (LOD) was $0.45 \mu \text{g}$ /mL. In addition, the optical fiber biosensor has good stability and selectivity, which makes it have broad application prospects in the early diagnosis of degenerative disease.
研究领域:
生物医学工程
·
传感技术
·
分子诊断
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
·
2018
·Vol. 2018
·期 39
·4375-4384
·
IF 2.507
Na An,Huiming Lin,Fengyu Qu
Graphical GNRs@mSiO 2 core–shell nanoparticles have been synthesized as host to load ICG and DOX molecules by the layer-by-layer assembly method through electrostatic interactions. A sensitive Se–Se linker was synthesized and grafted to ensure controlled release. Under NIR irradiation, the enhanced cytotoxicity to cancer cells is derived from the synergistic effect of chemotherapy, PTT, and PDT. A novel GNRs@mSiO 2 core–shell nanocomposite has been synthesized to integrate sensitive chemotherapy with photodynamic therapy (PDT) and photothermal therapy (PTT) for enhanced antitumor treatment by using gold nanorods (GNRs) as the core and mesoporous silica (mSiO 2 ) as the shell. After modifying with -NH 2 , Indocyanine Green (ICG, negative charge) and doxorubicin hydrochloride (DOX, positive charge) molecules could be stored in the mesopores by the layer-by-layer assembly method through electrostatic interactions. To inhibit leakage from the mesopores, a sensitive Se–Se linker was synthesized and adopted as a nanovalve coated on the outside of the nanoparticles (GNRs@mSiO 2 -ICG-DOX@Se-Se). Under NIR (808 nm) irradiation, heat elevation (derived from GNR/ICG) and generation of reactive oxygen species (ROS, derived from ICG) can also be detected in vitro. Owing to the redox sensitivity of Se–Se, GNRs@mSiO 2 -ICG-DOX@Se-Se showed reduction (GSH)- as well as oxidation (ROS)-triggered DOX release. To further ensure specific targeting, folic acid (FA) was grafted to the outside of the nanoparticles, because of the high expression of FA receptors on tumor cells. Detailed cell experiments performed with the nanoparticles and HepG2 as typical cancer cells showed enhanced cytotoxicity towards the cancer cells owing to the synergistic effect of chemotherapy, PTT, and PDT. On account of the different GSH concentrations in cancer and normal cells and the switch-on/off application of NIR irradiation, the GNRs@mSiO 2 -ICG-DOX@Se-Se-FA nanoparticles show potential for applications in cancer therapy.
研究领域:
纳米医学
·
肿瘤学
·
生物材料学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Hong Ke,Haifeng Sha,Yinfang Wang,Weiwei Guo,Xin Zhang,Zhiming Wang,Chusen Huang,Nengqin Jia
Electrochemiluminescent (ECL) assay has gradually drawn increasing interest in the biomedical analysis. This paper proposed a new methodology for ultrasensitive and facile detection of Alzheimer's disease marker β-amyloid (Aβ) by fabricating a sandwich-type ECL sensing platform. Herein, electrochemiluminescence resonance energy transfer (ECL-RET) was employed to determine Aβ concentration, which can be attributed to the quenching effect from RET between Ru(bpy) 3 2+ and gold nanorods (GNRs) acting as ECL-RET electron donor and acceptor, respectively. In this protocol, mesoporous carbon nanospheres were adopted to immobilize ECL reactant Ru(bpy) 3 2+ and antibody via nafion to acquire the RET donor nanocomposites (MOCs/nafion/Ru(bpy) 3 2+ /antibody), which were tightly interconnected with epoxy group functionalized Fe 3 O 4 nanoparticles . It is of vital importance that GNRs with exquisite rod shape were synthesized and exhibited a typical absorption peak at 650 nm to quench ECL signal of Ru(bpy) 3 2+ effectively. In addition, the ECL emission decreased linearly with the logarithm of Aβ concentration in a wide linear range from 1.0 × 10 −5 to 100 ng/mL with a detection limit of 4.2 × 10 −6 ng/mL. Furthermore, distinctive and desirable properties were verified to declare the promise for being applicable to analyze the Aβ content in real Alzheimer's cerebrospinal fluid samples with satisfactory results.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
·
诊断学
·
神经退行性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Yangqi Meng,Jian Yang,Rongyun Jiang,Shuyue Wang,Lihua Zheng,Guannan Wang,Xiao Tian,Hancheng Zhu,Duanting Yan,Chunguang Liu,Changshan Xu,Yongli Bao,Yuxue Liu
Currently, it is still a challenge to construct an integrated multi-functional nanoplatform with autofluorescence-free bioimaging and temperature sensing and photothermal therapy (PTT) for tumors located at 10 mm below the surface of local tissue (deep tissue) using gold nanorods (GNRs). Herein, through the self-assembly and surface modification of persistent luminescent nanoparticles (PLNPs) and GNRs using hexadecyl trimethyl ammonium bromide (CTAB) and phosphotungstic acid (PW 12 ) molecules, biocompatible PLNP-GNR composite nanoplatforms were constructed to simultaneously monitor autofluorescence-free bioimaging and temperature change during in vitro and in vivo PTT process using a single wavelength (635 nm) light excitation strategy. Especially, the nanoplatforms demonstrated that the local-tissue temperature can increase by 26 °C under 635 nm excitation (0.8 W·cm −2 ) and the photothermal conversion efficiency is ~37%. Among them, PLNPs were acted as optical probes for NIR bioimaging and ratiometric nanothermometer and the temperature sensitivity reaches to ~0.064 K −1 . This study is helpful for reducing photodamage to normal tissue through the accurate control of excitation light power during deep-tissue PTT process.
研究领域:
纳米医学
·
生物医学工程
·
癌症治疗
·
材料科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
·
2015
·Vol. 36
·期 5
·1282-1290
·
IF 2.088
Bin Gao,Lei Shen,Ke-Wu He,Wei-Hua Xiao
The aim of the present study was to examine the apoptosis of the hepatocellular carcinoma cell line, HepG2, induced by treatment with folic acid-conjugated silica-coated gold nanorods (GNRs@SiO2-FA) in combination with radiotherapy, and to determine the involvement of apoptosis-related proteins. An MTT colorimetric assay was used to assess the biocompatibility of GNRs@SiO2-FA. The distribution of GNRs@SiO2-FA into the cells was observed using transmission electron microscopy (TEM). HepG2 cells cultured in vitro were divided into the following 4 groups: i)the control group (untreated), ii) the GNRs@SiO2-FA group, iii) the radiotherapy group (iodine 125 seeds) and iv) the combination group (treated with GNRs@SiO2-FA and iodine 125 seeds) groups. The apoptosis of the HepG2 cells was detected by flow cytometry. The concentration range of <40 µg/ml GNRs@SiO2-FA was found to be safe for the biological activity of the HepG2 cells. GNRs@SiO2-FA entered the cytoplasm through endocytosis. The apoptotic rates of the HepG2 cells were higher in the GNRs@SiO2-FA and radiotherapy groups than in the control group (P<0.05). The apoptotic rate was also significantly higher in the combination group than the GNRs@SiO2-FA and radiotherapy groups (P<0.05). Taken together, these findings demonstrate that the combination of GNRs@SiO2-FA and radiotherapy more effectively induces the apoptosis of HepG2 cells. These apoptotic effects are achieved by increasing the protein expression of Bax and caspase-3, and inhibiting the protein expression of Bcl-2 and Ki-67. The combination of GNRs@SiO2-FA and radiotherapy may thus prove to be a new approach in the treatment of primary liver cancer.
研究领域:
癌症研究
·
纳米技术
·
分子生物学
·
放射生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioengineering & Translational Medicine
·
2024
·
IF 6.1
Qi Shang,Ziyan Chen,Jing Li,Mingmei Guo,Jiapei Yang,Zhu Jin,Yuanyuan Shen,Shengrong Guo,Feihu Wang
Chemotherapy treatment outcomes are severely restricted by multidrug resistance (MDR), in which tumors develop a multiple cross-resistance toward drug involving the pump and nonpump resistance mechanisms, resulting in drug efflux and defending against drug toxicity. Herein, we constructed a pH and near infrared (NIR) light responsive nanomedicine DOX@FG based on gold nanorods (GNRs) that demonstrated the potential to improve chemotherapy outcomes by overcoming MDR. DOX@FG was constructed by conjugating folic acid (FA) and doxorubicin (DOX) derivatives onto GNRs, where the DOX derivatives possessed an acid-labile hydrazone bond. Stimulated by the acidic media in endocytic organelles, DOX@FG exhibited a responsive dissociation for the controlled release of chemotherapeutic DOX. Surprisingly, we found the mild photothermal effect elicited by GNRs under NIR irradiation simultaneously inhibited the pump and nonpump resistance mechanisms, enhancing the intracellular DOX accumulation and sensitizing the cancer cells to DOX, collectively amplify the chemotherapy efficacy and delay the MCF-7/ADR breast tumor growth. This intelligent DOX@FG nanomedicine with the potential for two-pronged reversal of MDR may provide a prospective way to encourage chemotherapy efficacy.
研究领域:
纳米医学
·
癌症治疗
·
光热疗法
·
药物递送系统
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Yan Li,Junlin He,Jun Chen,Yazhen Niu,Yilin Zhao,Yuchan Zhang,Chao Yu
In this study, a dual-type responsive electrochemical immunosensor was developed for the quantitative detection of proprotein convertase subtilisin/kexin type 9 (PCSK9), a potential biomarker of cardiovascular disease in serum. N-doped graphene nanoribbons (N-GNRs) with good conductivity were used as the sensing matrix modifying the glassy carbon electrode. Palladium platinum alloy (PdPt) nanoparticles with high catalytic performance toward the reduction of hydrogen peroxide (H 2 O 2 ) were reduced onto amino-functionalized fullerene (n-C 60 -PdPt) and significantly amplified the electrochemical signal recorded by the amperometric i-t curve. Furthermore, staphylococcus protein A (SPA) with antibody orientation function was introduced to improve the immunoreaction efficiency. Accordingly, a label-free immunosensor was fabricated based on n-C 60 -PdPt/N-GNRs for the quick detection of PCSK9. Meanwhile, to realize ultrasensitive detection of PCSK9, Pt-poly (methylene blue) (Pt-PMB) nanocomposites synthesized by a one-pot method for the first time were used as a novel signal label, which exhibited uniform morphology as well as good conductivity and produced an electrochemical signal recorded by differential pulse voltammetry (DPV). Herein, a novel sandwich-type immunosensor was designed using n-C 60 -PdPt/N-GNRs as the sensing matrix and Pt-PMB as the signal label for sensitive detection of PCSK9. Under optimal conditions, the label-free immunosensor showed a linear range of 10 pg mL −1 to 100 ng mL −1 with a detection limit of 3.33 pg mL −1 (S/N=3), and the sandwich-type immunosensor exhibited a linear range of 100 fg mL −1 to 100 ng mL −1 with a detection limit of 0.033 pg mL −1 (S/N=3) for PCSK9 detection, indicating its potential application in clinical bioassay analysis.
研究领域:
生物医学工程
·
分析化学
·
临床检验
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Zhiyong Tang,Junlin He,Jun Chen,Yazhen Niu,Yilin Zhao,Yuchan Zhang,Chao Yu
We are presenting an electrochemical immunosensor for the determination of Galectin-3 (Gal-3), a biomarker of heart failure. A glassy carbon electrode (GCE) was modified with a film of a composite made from the N-doped graphene nanoribbons immobilized Fe-based-Metal-organic frameworks deposited with Au nanoparticles (N-GNRs-Fe-MOFs@AuNPs). Primary antibody against Gal-3 (Gal-3-Ab 1 ) was immobilized on the Au nanoparticles on the surface of the modified GCE which then was blocked with bovine serum albumin . Signal amplification is crucial for obtaining low detection limits in biosensors. Here, a relatively simple and ultrasensitive sandwich-type electrochemical immunosensor based on novel signal generation and amplification was developed for the determination of Gal-3. A kind of novel redox-active species, AuPt-Methylene blue (MB) (AuPt-MB) nanocomposites , was synthesized by a one-pot method for the first time. Wherein, MB as a kind of the electron transfer mediators in an amperometric biosensor is responsible for electron production and signal amplification. The rod-like AuPt-MB nanohybrids displayed uniform morphology and good electrochemical activity and can combine with the second antibodies against Gal-3 (Gal-3-Ab 2 ). And the AuPt-MB-Ab 2 coupled with the N-GNRs-Fe-MOFs@AuNPs-Ab 1 to form the sandwich type format that can greatly enhance the biosensor's sensitivity. Under optimal conditions, the designed immunosensor exhibited a linear concentration range from 100 fg mL −1 to 50 ng mL −1 , with a low detection limit of 33.33 fg mL −1 (S/N = 3) for Gal-3 in spiked serum. Additionally, the designed immunosensor showed acceptable selectivity, reproducibility and stability. The satisfactory results in analyzing human serum samples indicated potential application promising in monitoring biomarkers.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Ping Tang,Meishuang Xing,Xinyue Xing,Qiao Tao,Wendai Cheng,Shengde Liu,Xiaoxu Lu,Liyun Zhong
Phototherapy, especially the photothermal therapy (PTT) and the photodynamic therapy (PDT), have become very promising in cancer treatment due to its low invasiveness and high efficacy. Both PTT and PDT involve the utilization of light energy, and their synergistic treatment should be a good solution for cancer treatment by ingenious design. The therapeutic effect of phototherapy is closely associated with the amount and location of anticancer-nanodrugs accumulated in tumor cells, and the receptor-mediated endocytosis should be an excellent candidate for enhancing anticancer-nanodrugs internalization. Surface enhanced Raman spectroscopy (SERS) imaging is suitable for tracing nanodrugs due to its high selectivity, sensitivity and reliability. In this paper, we hope to construct a receptor-mediated PTT/PDT synergistic anticancer nanodrugs and evaluate the corresponding efficacy through SERS tracing function. Here, the receptor-mediated PTT/PDT synergistic anticancer nanodrugs are prepared by the chemical modification of gold nanorods (GNRs), involving protoporphyrin IX (PpIX), 4-mecaptobenzoic acid (MBA), and folic acid (FA). The achieved results show that the receptor-mediated endocytosis can greatly facilitate the internalized amount and intracellular distribution of the nanodrugs, thus lead to the anti-cancer efficacy improvement. Importantly, this receptor-mediated PTT/PDT synergistic treatment with SERS tracing function will provide a simple and effective strategy for the design and application of anticancer phototherapy nanodrugs.
研究领域:
癌症治疗
·
纳米技术
·
生物医学工程
·
光学成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）