APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2017
·Vol. 83
·期 10
·
IF 3.807
Lei Yu,Ming-yue Cao,Peng-tao Wang,Shi Wang,Ying-rong Yue,Wen-duo Yuan,Wei-chuan Qiao,Fei Wang,Xin Song
Biohydrogen production from the pulp and paper effluent containing rich lignocellulosic material could be achieved by the fermentation process. Xylose, an important hemicellulose hydrolysis product, is used less efficiently as a substrate for biohydrogen production. Moreover, azo dyes are usually added to fabricate anticounterfeiting paper, which further increases the complexity of wastewater. This study reports that xylose could serve as the sole carbon source for a pure culture of Klebsiella oxytoca GS-4-08 to achieve simultaneous decolorization and biohydrogen production. With 2 g liter−1 of xylose as the substrate, a maximum xylose utilization rate (URxyl) and a hydrogen molar yield (HMY) of 93.99% and 0.259 mol of H2 mol of xylose−1, respectively, were obtained. Biohydrogen kinetics and electron equivalent (e− equiv) balance calculations indicated that methyl red (MR) penetrates and intracellularly inhibits both the pentose phosphate pathway and pyruvate fermentation pathway, while methyl orange (MO) acted independently of the glycolysis and biohydrogen pathway. The data demonstrate that biohydrogen pathways in the presence of azo dyes with sulfonate and carboxyl groups were different, but the azo dyes could be completely reduced during the biohydrogen production period in the presence of MO or MR. The feasibility of hydrogen production from industrial pulp and paper effluent by the strain if the xylose is sufficient was also proved and was not affected by toxic substances which usually exist in such wastewater, except for chlorophenol. This study offers a promising energy-recycling strategy for treating pulp and paper wastewaters, especially for those containing azo dyes.IMPORTANCE The pulp and paper industry is a major industry in many developing countries, and the global market of pulp and paper wastewater treatment is expected to increase by 60% between 2012 and 2020. Such wastewater contains large amounts of refractory contaminants, such as lignin, whose reclamation is considered economically crucial and environmentally friendly. Furthermore, azo dyes are usually added in order to fabricate anticounterfeiting paper, which further increases the complexity of the pulp and paper wastewater. This work may offer a better understanding of biohydrogen production from xylose in the presence of azo dyes and provide a promising energy-recycling method for treating pulp and paper wastewater, especially for those containing azo dyes.
研究领域:
环境科学
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微生物学
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PubMed
高影响因子
BIOMATERIALS
·
2024
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IF 12.8
Bingxu Zhang,Xujian Li,Chuan Jiang,Chuanguang Wang,Haifeng Que,Cheng Zheng,Zhixiao Ji,Xudong Tao,Hongtao Xu,Changcan Shi
The effective prevention and treatment of anastomotic leakage after intestinal anastomosis for colorectal diseases is still a major clinical challenge. In order to assist intestinal anastomosis healing and avoid anastomotic leakage caused by high tension, low blood supply or infection, we designed a double-layer nanofiber intestinal anastomosis scaffold, which was composed of electrospun PTMC/PHA nanofibers as the main layer, and electrospun PVA/OHA-Gs nanofibers with antibacterial properties as the antibacterial surface layer. This double-layer scaffold has good toughness, its maximum tensile force value could reach 8 N, elongation could reach 400 %, and it has hydrophilic properties, and its contact angle was about 60°. On the basis of reducing anastomotic tension and isolating intestinal contents, this double-layer nanofiber anastomotic scaffold not only played an antibacterial effect in the short term after surgery to reduce inflammatory response, but also had the characteristics of multiple three-dimensional network structure like extracellular matrix which could promote tissue healing. The PVA/OHA-Gs@PTMC/PHA scaffold was implanted into a rabbit model simulating mechanical intestinal obstruction, and the results showed that the nanofibers of the scaffold could be degraded in vivo while maintaining a certain stability, that is, the overall structure of the PVA/OHA-Gs@PTMC/PHA scaffold would not shrink and deform due to degradation in a certain period of time. Therefore, the treatment with this scaffold showed better healing at the anastomotic site. Compared to the direct anastomosis group and pure PTMC scaffold group, the double-layer scaffold group promoted a faster return to normal anastomotic strength within 7 days. This PVA/OHA-Gs@PTMC/PHA double-layer nanofiber flexible scaffold appears to be a promising therapeutic strategy to prevent anastomotic leakage after intestinal anastomosis.
研究领域:
生物医学工程
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组织工程
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外科手术研究
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 10
·1-11
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IF 5.7
Tian Jiangman,Zhu Qihao,Huang Xiaojing,Li Yuan
A novel sandwich-type “on-off” electrochemiluminescence (ECL) biosensor for the determination of α(2,3)-sial-Gs was designed. Specifically, amino-functionalized porous silica nanoparticles (HPSNs-NH 2 ) were first prepared and then decorated with gold nanoparticles (Au NPs) to form HPSNs-NH 2 @Au NP nanocomposite, which exhibited a strong ability to enhance ECL intensity with K 2 S 2 O 8 as co-reactant (signal-on) and could immobilize the target-specific binding molecules of maackia amurensis lectin (MAL). Additionally, AuPdPt trimetallic nanoparticles were prepared to serve as a quenched ECL signal indicator (signal-off) with the ability of capturing the target non-specific binding molecules of 3-aminophenylboronic acid (APBA) to form a signal label. The sandwich-type ECL biosensor was constructed based on the structure of MAL-α(2,3)-sial-Gs-APBA and achieved a determination toward α(2,3)-sial-Gs with a wide linear range from 1 fg mL −1 to 10 ng mL −1 and a low detection limit of 0.5 fg mL −1 . Furthermore, the proposed ECL biosensor showed satisfactory selectivity, stability, and reproducibility for α(2,3)-sial-Gs determination. Graphical Abstract
研究领域:
分析化学
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生物医学工程
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纳米科技
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PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2023
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IF 7.7
Jialing Tian,Yi Han,Pengna Yin,Jianbing Zhang,Tingting Guo,Haibo Li,Yanan Hou,Yuanyuan Song,Jianbo Guo
Under high-strength perchlorate concentration ranging from 300 to 1300 mg/L, the dissimilatory perchlorate reducing granular sludge (DPR-GS) was successfully cultivated in an up-flow anaerobic sludge blanket (UASB) reactor to investigate performance and starvation recovery for treating perchlorate wastewater. The mature DPR-GS had excellent perchlorate removal efficiency (98.47%) at 1200 mg/L. Microbial community analysis suggested that the dominant genera in mature DPR-GS were Sulfurospirillum and Acinetobacter . The DPR-GS system conformed well to the modified Stover-Kincannon model with the maximum substrate utilization (U max ) of 90.01 kg/(m 3 ·d) and the saturation value constant (K B ) of 83.93 kg/(m 3 ·d). Additionally, the starvation recovery of DPR-GS system was first investigated. The perchlorate removal efficiency recovered to 91.39% after 8 days under the 30-day starvation stress, and the microbial activity also recovered to normal condition. The three-dimensional excitation-emission matrix (3D-EEM) proved that the tryptophan-like component in extracellular polymeric substance (EPS) increased during the starvation period, which was associated with immune functions and played an important role in maintaining the microbial activity. Meanwhile, the secretion of humic acid-like component in EPS promoted electron transfer efficiency, thus improving microbial metabolic activity during the recovery period. Further analysis by the parallel factor (PARAFAC) of 3D-EEM confirmed that the decrease of immune substances and the increase of metabolic products in EPS during the starvation recovery period, which enhanced the recovery of microbial activity and the improvement of perchlorate removal performance. This study revealed the response of the DPR-GS system to high-strength perchlorate and starvation stress, thus provided a further basis of perchlorate tolerance and starvation recovery for the application of DPR-GS system to remove high-strength concentrated perchlorate from contaminated water .
研究领域:
环境工程
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微生物学
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水处理技术
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高影响因子
Nature Chemical Biology
·
2022
·Vol. 18
·期 11
·1196-1203
·
IF 16.174
Lin Hui,Xiao Peng,Bu Rui-Qian,Guo Shengchao,Yang Zhao,Yuan Daopeng,Zhu Zhong-Liang,Zhang Chuan-Xin,He Qing-Tao,Zhang Chao,Ping Yu-Qi,Zhao Ru-Jia,Ma Chuan-Shun,Liu Chang-Hao,Zhang Xiao-Ning,Jiang Dan,Huang Shaohui,Xi Yue-Tong,Zhang Dao-Lai,Xue Chen-Yang,Yang Bai-Sheng,Li Jian-Yuan,Lin Hao-Cheng,Zeng Xu-Hui,Zhao Han,Xu Wen-Ming,Yi Fan,Liu Zhongmin,Sun Jin-Peng,Yu Xiao
Adhesion G protein-coupled receptors are elusive in terms of their structural information and ligands. Here, we solved the cryogenic-electron microscopy (cryo-EM) structure of apo-ADGRG2, an essential membrane receptor for maintaining male fertility, in complex with a G s trimer. Whereas the formations of two kinks were determinants of the active state, identification of a potential ligand-binding pocket in ADGRG2 facilitated the screening and identification of dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate and deoxycorticosterone as potential ligands of ADGRG2. The cryo-EM structures of DHEA–ADGRG2–G s provided interaction details for DHEA within the seven transmembrane domains of ADGRG2. Collectively, our data provide a structural basis for the activation and signaling of ADGRG2, as well as characterization of steroid hormones as ADGRG2 ligands, which might be used as useful tools for further functional studies of the orphan ADGRG2.
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高影响因子
AQUACULTURE
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2025
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IF 3.9
Letian Li,Yuan Wang,Laizhuang Jin,Lei Feng,Qianhui Song,Di Fang,Na Ying,Xincang Li,Wenhong Fang
The gastric sieve (GS) is a filtering structure found in the posterior stomach chamber (PSC) of Litopenaeus vannamei , which allows small particles to enter the hepatopancreas directly. However, it remains unclear if pathogens can also enter the hepatopancreas through this pathway. In this study, we examined the internal structural changes of the PSC via histological and ultra-structure analysis. We also orally administered fluorescent microspheres and bioparticles ( Vibrio parahaemolyticus and WSSV) of varying sizes to shrimp to determine their passage under hypoxia stress. The results showed that the PSC could be divided into four segments based on structural changes, and the GS performed its filtering function through two kinds of setae with different morphologies. Under normal dissolved oxygen levels (approximately 7.6 mg/L), V. parahaemolyticus and 1 μm fluorescent microspheres were unable to enter the hepatopancreas via GS. In contrast, under hypoxia stress (1.5–2 mg/L), both vibrio and 1 μm fluorescent microspheres could enter the hepatopancreas via GS in a short timeframe. WSSV and 0.1 μm fluorescent microspheres could enter the hepatopancreas via GS irrespective of hypoxia stress, while 5 μm fluorescent microspheres could not enter the hepatopancreas via GS even under hypoxia. These results suggest that GS functions as a particle diameter-dependent filter, with its filtering function influenced by hypoxia stress. Pathogens around 1 μm in size can invade the hepatopancreas through this pathway under hypoxia stress, providing novel insights into the mechanisms of pathogen invasion in shrimp.
CAS / MDL:
研究领域:
水产养殖学
·
海洋生物学
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DOI
高影响因子
Gland Surgery
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2022
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IF 2.16
Meili Zhang,Jialei Gu,Wendong Wang,Kejing Wang,Linfeng Zheng,Jianguo Feng,Jinbiao Shang
Background Identifying the high recurrence group of patients with early-stage papillary thyroid cancer (PTC) is the greatest challenge in the management of this disease. It has been noted that B-type Rafkinase (BRAF) V600E mutation and programmed death ligand 1 (PD-L1) are associated in PTC and highly expressed in PTC, correlating in PTC as potential prognostic biomarkers. However, whether they can be used to predict the aggressiveness and recurrence of early PTC remains unclear. Methods Clinicopathological data of 137 patients with early PTC [tumor-node-metastasis (TNM) stage I–II] who underwent surgery in Zhejiang Cancer Hospital between 2008 and 2010 were retrospectively analyzed. BRAF V600E mutation and PD-L1 was detected by immunohistochemistry. The median follow-up time was 136 months (interquartile range 5.8). The presence of tumor confirmed by imaging or pathology or lymph node metastasis was considered as tumor recurrence. The association of both alone and in combination with clinicopathological features and recurrence was statistically analyzed respectively. The risk of recurrence was assessed using Cox regression models. Results Most of the 137 early PTC were female (78.1%). The mean age was 43.2±12.1 years. The median tumor size was 1.4 cm; 14 patients developed recurrence during follow-up period; 56 patients (40.9%) were detected positive for BRAF V600E mutation; 76 patients (55.5%) were detected positive for PD-L1. Patients with both BRAF V600E mutation and PD-L1 expression had larger tumors (P=0.038), were more likely to have extrathyroidal invasion (P=0.045), and had a lower rate of cervical lymph node metastasis (P=0.046). The recurrence rate was 17.5% (7/40) in patients with BRAF V600E mutation and PD-L1 double expression compared to 8.9% (4/45) in patients with BRAF V600E mutation and PD-L1 double negative [hazard ratio (HR) =1.267; 95% CI: 0.841–1.909; P=0.257]. Survival curves showed flatter recurrence-free survival (RFS) curves in positive BRAF V600E mutation only and PD-L1 expression only, whereas decreased sharply in positive expression of both BRAF V600E mutation and PD-L1; however, the differences were not significant (P>0.05). Conclusions The combination of BRAF V600E mutation and PD-L1 to identify group at higher risk of recurrence in early PTC has insufficient clinical evidence and should be used with caution in the clinical management of PTC.
研究领域:
肿瘤学
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病理学
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分子生物学
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PubMed
高影响因子
International Journal of Electrochemical Science
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2023
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IF 1.5
Yu Zhang,Qiang Xie,Zhi Xia,Guofeng Gui,Ping Zhang,Lifen Meng,Ling Pan
In this study, a rapid and sensitive electrochemical sensor based on amino β-cyclodextrins (NH 2 -β-CD) functionalized graphene and multi-walled carbon nanotubes (MWCNTs) nanohybrids was successfully constructed for the simultaneous determination of p -aminophenol ( p -AP) and acetaminophen (AC). Graphene and MWCNTs were oxidized with strong acid to give carboxyl groups (COOH-f-GS/MWCNTs). Then, the NH 2 -β-CD was immobilized on its surface by EDC and NHS to prepare a porous hybrid nanocomposite (β-CD-NHCO-f-GS/MWCNTs). The composite material displays a well synergistic effect of high electrocatalytic activity and excellent conductivity of COOH-f-GS/MWCNTs and high supramolecular recognition capability of NH 2 -β-CD. The electrocatalytic mechanism and catalytic process are discussed. Under the optimum conditions, the detection limits of the as-prepared electrochemical sensor are 0.11 mM and 0.15 mM for p -AP and AC, respectively. The linear ranges are 1.0 mM to 1.32 mM for p -AP and 0.6 mM to 0.44 mM for AC. More importantly, this result was measured in the presence of each other. The electrochemical sensor can simultaneously display obvious and accurate response to p -AP and AC at different potentials with high sensitivity and less interferences.
研究领域:
分析化学
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材料科学
·
药物分析
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高影响因子
Journal of Materials Chemistry C
·
2024
·Vol. 12
·期 37
·15012-15023
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IF 5.7
Zengmou Li,Qing Zhao,Keyu Zhang,Dingfang Cui,Keqi Chen,Yusheng Gong,Shaoze Zhang,Yin Li,Junxian Hu,Bin Yang,Yaochun Yao
Iron oxalate (FeC2O4) is a promising candidate as an anode material for lithium-ion batteries (LIBs) with its advantages of low cost and high capacity. However, its commercial application is limited by its poor electrical conductivity, which leads to slow chemical reaction kinetics. Herein, a novel FeC2O4-based composite (FeC2O4/Gs) with dual-level structured graphene sheets (Gs) was constructed via electrostatic adherence technology, which enhanced the combination form and composite effect for two discrepant materials. Based on the dual-level structured Gs in FeC2O4/Gs composites, micro-based Gs (∼5 μm) was dispersed between the FeC2O4 particles. Meanwhile, the nano-based (∼50 nm) Gs was coated and embedded on the surface of rod-like FeC2O4 particles. Because of the special structure of FeC2O4/Gs, it has a dual promotion of electron transport both within and on the surface of the FeC2O4 particles, favoring electrochemical activity and efficiently inducing the conversion reaction. Consequently, the FeC2O4/Gs composite achieved a high reversible capacity of 820 mA h g−1 and excellent cycling stability with a capacity retention of 41% after 200 cycles under 10C. This study provides a novel opportunity to design composites and electrodes with electronic conductivity, excellent electrochemical activity and ultrahigh cycling stability for lithium storage.
研究领域:
材料科学
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能源存储
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DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2025
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IF 1.6
Zhao Fan,Zhang Wei,Chen Yuantao
The zinc-based metal–organic framework (IRMOF-3) was assembled on the surface of Galdieria sulfuraria (Gs) via a one-pot method to fabricate the composite adsorbent Gs/IRMOF-3. Results show that the material achieves optimal adsorption performance at pH = 6. The adsorption process follows the Freundlich isotherm model and pseudo-second-order kinetic model, demonstrating that it is dominated by multi-molecular layer chemical adsorption and represents a spontaneous endothermic process. This study provides a novel strategy for developing efficient and eco-friendly uranyl adsorption materials.
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高影响因子
Journal of Environmental Chemical Engineering
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2024
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IF 7.4
Jinyang Chen,Juanli Wu,Ping Liu,Li Gan,Zuliang Chen
Efficient removal and recovery of heavy metals (HMs) from acid mine drainage (AMD) is crucial for both protecting mine ecosystems and reclaiming valuable HMs resources. In this study, biohybrid with ferrous sulfide nanoparticles ( n FeS@GS) were biosynthesized via Geobacter sulfurreducens (GS) and employed for the removal and recovery of HMs from AMD. Initially, n FeS@GS were successfully synthesized as confirmed by TEM and exhibited high efficiencies in removing Cu 2+ and Pb 2+ in solution, respectively. This occurred because bio- n FeS and GS cells synergistically enhanced the removal of Cu 2+ and Pb 2+ . What is more, the removal efficiencies of various HMs from real AMD using n FeS@GS remained high, with documented rates of 87.9 % for Cu 2+ , 96.2 % for Pb 2+ , 95.1 % for Cd 2+ , 81.4 % for Ni 2+ , 80.9 % for Mn 2+ , and 91.9 % for Zn 2+ . Subsequent characterization of the removal function through 3D-EEM, Bio-TEM, XRD, FTIR and XPS analyses revealed substantial organic capping agents derived from GS adhering to the bio- n FeS, forming surface complexes with Cu 2+ and Pb 2+ , and Cu 2+ was reduced to Cu + /Cu 0 . Zeta potential measurements, adsorption kinetics and reduction kinetics supported that the removal mechanism involved electrostatic interactions, surface complexation, ion exchange and reduction. Finally, n FeS@GS desorption rates of HMs exceeded 90.7 %, highlighting the efficacy of n FeS@GS for recovering HMs from AMD. These findings provide valuable and practical insights into the removal and recovery of HMs from AMD using biomaterials synthesized from microorganisms.
研究领域:
环境科学
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微生物学
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高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-11
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IF 5.228
Yu Lei,Tang Qing-wen,Zhang Yu-jia,Chen Rong-ping,Liu Xin,Qiao Wei-chuan,Li Wen-wei,Ruan Hong-hua,Song Xin
In this work, the effect of cultivation factors on the flocculation efficiency (FE) of bioflocculant P-GS408 from Klebsiella oxytoca was optimized by the response surface methodology. The most significant factor, i.e . culture time, was determined by gray relational analysis. A total of 240 mg of purified P-GS408 was prepared from 1 liter of culture solution under the optimal conditions. GC-MS analysis results indicated that the polysaccharide of P-GS408 mainly contains Rhamnose and Galactose, and the existence of abundant hydroxyl, carboxyl and amino groups was evidenced by FTIR and XPS analyses. With the aid of Fe 3+ , the FE of kaolin solution by P-GS408 could achieve 99.48% in ten minutes. Functional groups of polysaccharide were involved in the first adsorption step and the zeta potential of kaolin solution changed from −39.0 mV to 43.4 mV in the presence of Fe 3+ and P-GS408. Three-dimensional excitation-emission (EEM) fluorescence spectra demonstrates that the trivalent Fe 3+ and Al 3+ can bind efficiently with P-GS408, while those univalent and divalent cations cannot. With the help of SEM images, FTIR, zeta potential and EEM spectra, we proposed the P-GS408 flocculation mechanism, which consists of coordination bond combination, charge neutrality, adsorption and bridging, and net catching.
研究领域:
微生物学
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环境科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）