ANALYTICAL SCIENCES
·
2019
·Vol. 35
·期 10
·1135-1140
·
IF 1.618
Wang Ze,Dong Bin,Feng Guodong,Shan Hongyan,Huan Yanfu,Fei Qiang
In the present study, we synthesized a water-soluble substance (Hemin-mPEG) at room temperature by using hemin and poly(ethylene glycol) methyl ether (mPEG). It was found that the Hemin-mPEG maintained the excellent catalytic activity inherited from hemin, and was first used to catalyze a luminol–H 2 O 2 chemiluminescence (CL) system to generate an intense and slow CL signal. The results of a mechanism research showed that the presence of Hemin-mPEG could promote the production of oxygen-relative radicals from H 2 O 2 and dissolved oxygen in solution. Based on this mechanism, an ultra-sensitive, cheap and simply practical sensor for detecting glucose and H 2 O 2 was developed. Under the most optimal experimental conditions, H 2 O 2 and glucose detection results exhibited a good linear range from 0.002 to 3 μM and from 0.02 to 4 μM, respectively, and the detection limits were 1.8 and 10 nM, respectively. This approach has been successfully used to detect glucose in actual biological samples, and achieved good results.
研究领域:
生物化学
·
分析化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-16
·
IF 5.3
Li Guiyin,Guo Fei,Liang Jianlu,Wan Bingbing,Liang Jintao,Zhou Zhide
A new sandwich-type electrochemical biosensing platform was developed by gold @polyphthalenediamine nanohybrids (AuNP@PoPD) as the sensing platform and phosphorus doped reduced graphene oxide-hemin-palladium nanoparticles (PrGO-Hemin-PdNP) as the signal amplifier for phosphatidylinositol proteoglycan 3 (GPC3). AuNP@PoPD, co-electrodeposited into the screen printed electrode with high conductivity and stability, is dedicated to assembling the primary GPC3 aptamer (GPC3 Apt ). The second GPC3 Apt immobilized on the high conductivity and large surface area of PrGO-Hemin-PdNP was utilized as an electrochemical signal reporter by hemin oxidation (PrGO-Hemin-PdNP-GPC3 Apt ). In the range 0.001–10.0 ng/mL, the hemin oxidation current signal of the electrochemical aptasensor increased log-linearly with the concentration of GPC3, the lowest detection limit was 0.13 pg/mL, and the sensitivity was 2.073 μA/μM/cm 2 . The aptasensor exhibited good sensing performance in a human serum sample with the relative error of 4.31–8.07%. The sandwich sensor showed good selectivity and stability for detection GPC3 in human serum samples, providing a new efficient and sensitive method for detecting HCC markers. Graphical Abstract
研究领域:
生物医学工程
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分析化学
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肿瘤学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Xiaohong Tan,Hao Lin,Jiahao Zhou,Zhanhua Su,Bingbing Wan,Xiangming Li,Zhongqun Zhou,Yuge Liu,Guiyin Li
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality worldwide. Early detection of HCC is pivotal for early treatment, thereby improving survival rates. Golgi protein 73 (GP73) is a promising biomarker for early HCC diagnosis, making it crucial to develop a sensitive method to detect GP73. In this study, a novel ratiometric electrochemical aptasensor was fabricated for the sensitive detection of GP73 using Hemin-Cu-based metal-organic framework (Hemin-Cu-MOF) nanocomposites and GP73 aptamers. The Hemin-Cu-MOF nanocomposites possessed not only excellent electroactive property with the Hemin and Cu-MOF as dual signal outputs, but also good electroconductivity to amplify the electrochemical signals. The Hemin-Cu-MOF nanocomposites with numerous active sites was used to label the GP73 aptamer (AptI) and construct the Hemin-Cu-MOF-AptI signal probe. Another sulfhydryl-modified GP73 aptamer (AptⅡ) was immobilized on the gold nanoparticles (Au) modified screen-printed carbon electrode (Au/SPCE) through gold-sulfur (Au-S) bonds and used as a capture probe. Upon adding GP73, AptI and AptⅡ specifically recognized GP73 to form the sandwich complex AptⅡ@GP73@Hemin-Cu-MOF-AptI, making the dual signal of Hemin-Cu-MOF change and indicating GP73 level. Under optimal experimental conditions, the ratio of two oxidation peak currents (I Cu-MOF /I Hemin ) was proportional to the GP73 concentration (1.0–300.0 ng/mL), with a detection limit as low as 0.625 ng/mL. Furthermore, the aptasensor was tested in real human serum samples using the standard recovery method, with recoveries ranging from 104.10 % to 116.66 %. Thus, the proposed aptasensor has great potential in clinical analysis for detecting GP73 and early diagnosing of HCC.
研究领域:
生物医学工程
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肿瘤学
·
分析化学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Wei-Jia Zeng,Ni Liao,Yan-Mei Lei,Jing Zhao,Ya-Qin Chai,Ruo Yuan,Ying Zhuo
In this work, hemin was firstly used as electrochemically regenerable co-reaction accelerator for signal amplification to develop an ultrasensitive aptasensor for Aflatoxin M1 (AFM1) detection. Initially, the perylenetetracarboxylic acid (PTCA) was directly employed as luminophore to construct the ECL sensing nano-platform by combining Au nanoparticles (Au NPs) for immobilizing thiol-terminated hairpin probe (H1). Then with the help of hairpin H2, H3, the AFM1-catalyzed hairpin assembly (CHA) was executed to produce the H1-H3 duplex, which could further initiate the hybridization chain reaction (HCR) to generate dendritic DNA polymers consisting of G-rich sequence for capturing large quantities of hemin on the electrode surface . Herein, hemin as electrochemically regenerable co-reaction accelerator could interact with the co-reactant (S 2 O 8 2− ) to obviously improve the luminous efficiency of the PTCA. Therefore, a strong and stable ECL signal was achieved by the employment of hemin as electrochemically regenerable co-reaction accelerator. The proposed aptasensor determined AFM1 down to 0.09 pg mL −1 within a linear range of 0.4 pg mL −1 to 400 ng mL −1 . With excellent sensitivity and stability, the strategy provided an efficient and simple method for the trace detection of biomolecules in clinical analysis.
研究领域:
分析化学
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生物医学工程
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DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 29
·期 1
·146
·
IF 4.6
Zitao Yang,Cunhao Luo,Ning Wang,Junshao Liu,Menglong Zhang,Jing Xu,Yongnan Zhao
The high power density and long cyclic stability of N-doped carbon make it an attractive material for supercapacitor electrodes. Nevertheless, its low energy density limits its practical application. To solve the above issues, Fe2O3embedded in N-doped porous carbon (Fe2O3/N-PC) was designed by pyrolyzing Hemin/activated carbon (Hemin/AC) composites. A porous structure allows rapid diffusion of electrons and ions during charge–discharge due to its large surface area and conductive channels. The redox reactions of Fe2O3particles and N heteroatoms contribute to pseudocapacitance, which greatly enhances the supercapacitive performance. Fe2O3/N-PC showed a superior capacitance of 290.3 F g−1at 1 A g−1with 93.1% capacity retention after 10,000 charge–discharge cycles. Eventually, a high energy density of 37.6 Wh kg−1at a power density of 1.6 kW kg−1could be delivered with a solid symmetric device.Keywords:Hemin/AC;Fe2O3/N-PC;pseudocapacitance;quasi-solid-state supercapacitor
研究领域:
材料科学
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电化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 5
·1-8
·
IF 6.232
Lin Yanna,Sun Yuanling,Dai Yuxue,Zhu Xiaodong,Liu Hao,Han Rui,Gao Dandan,Luo Chuannan,Wang Xueying
Lysozyme aptamer-functionalized magnetic alginate hydrogel was prepared for separation and enrichment of lysozyme. Luminol-labeled aptamer was used as a signal tag, and the signal tag was adsorbed on magnetic carboxylated carbon nanotubes based on the π-interaction. When lysozyme was added, the aptamer specifically binds to the lysozyme, causing the signal tag to detach from the magnetic carboxylated carbon nanotubes. When the aptamer/lysozyme complex bound to the complementary single strand of aptamer on the hemin@HKUST-1, lysozyme was released. The released lysozyme can be recombined with the signal tag adsorbed on the magnetic carboxylated carbon nanotube, allowing more signal tag to be dispersed into the solution. Determination of lysozyme was achieved by releasing the luminol-labeled aptamer to generate a chemiluminescence signal at a wavelength of 425 nm. It was proved by experiments that the synthesized hemin@HKUST-1 had a strong catalytic effect on the luminol-NaOH-H 2 O 2 system. The chemiluminescence signal was increased nearly 100 times. The complementary pairing allowed the luminol to be immobilized on the surface of hemin@HKUST-1. The generation and consumption of short-lived reactive oxygen species were concentrated on the surface of the MOFs, which improves the chemiluminescence efficiency. The introduction of hemin@HKUST-1 and DNA solved the defects of chemiluminescence analysis. The chemiluminescence assay was able to detect lysozyme with linear range of 1.05 × 10 −6 U∙mg −1 (6.00 × 10 −13 mol∙L −1 )–1.25 × 10 −2 U∙mg −1 (7.14 × 10 −9 mol∙L −1 ); the detection limit was 3.50 × 10 −7 U∙mg −1 (2.00 × 10 −13 mol∙L −1 ) ( R 2 = 0.99). The recovery of lysozyme in spiked saliva samples was 97.4–102.8%.
Schematic presentation of chemiluminescence assay. Lysozyme (Lys) was captured by aptamer-modified magnetic sodium alginate (M-Alg-Apt); Glycine (pH = 2) as eluent for Lys. Luminol-modified Apt (Apt-luminol) as signal tag; magnetic carbon nanotubes (MCNTs) as adsorption matrix; cDNA was complementary to Apt; hemin@HKUST-1 as catalyst.
研究领域:
生物化学
·
分析化学
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PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Mingdi Xu,Zhuangqiang Gao,Qiaohua Wei,Guonan Chen,Dianping Tang
A new signal-amplification strategy based on copper(II) (Cu 2+ )-dependent DNAzyme was developed for sensitive impedimetric biosensing of Cu 2+ in aqueous solution by coupling with target-induced formation of hemin/G-quadruplex-based DNAzyme and enzymatic catalytic precipitation technique. Initially, the target analyte cleaved the Cu 2+ -specific DNAzyme to generate an initiator strand on the sensor. Thereafter, the initiator strand underwent an unbiased strand-displacement reaction between hairpin probes in turn to construct a nicked double-helix, accompanying the formation of hemin/G-quadruplex DNAzyme on the long duplex DNA . The newly formed DNAzyme could oxidize the 4-chloro-1-naphthol (4-CN) to produce an insoluble precipitation on the sensor, thus resulting in a local alteration of the conductivity. Under the optimal conditions, the resistance increased with the increasing Cu 2+ in the sample, and exhibited a wide dynamic working range from 0.1 pM to 5.0 nM with a detection limit of 60 fM. The methodology also displayed a high selectivity for Cu 2+ relative to other potentially interfering ions owing to the highly specific Cu 2+ -dependent DNAzyme, and was applicable for monitoring Cu 2+ in real river samples. Thus, our strategy has a good potential in the environment surveys.
研究领域:
生物化学
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分析化学
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环境科学
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DOI
PubMed
高影响因子
TALANTA
·
2013
·
IF 3.498
Yuezhen He,Xiaoxun Wang,Yuzhong Zhang,Feng Gao,Yongxin Li,Hongqi Chen,Lun Wang
In this work, we reported a sensitive chemiluminescent immunosensor for the detection of human leptin by using hemin/G-quadruplex DNAzymes to amplify detection signal. In this sensing system, the primary antibody (anti-human leptin) was firstly bound to the 96-well plates, and human leptin and biotinylated secondary antibody were successively combined to form sandwich-type immune complex through specific interactions. Then streptavidin labeled with hemin/G-quadruplex DNAzymes was assembled to the sandwich-type immunocomplex by streptavidin–biotin interaction. The DNAzymes exhibited an excellent catalytic activity to the chemiluminescent reaction of luminol with hydrogen peroxide in strong alkaline solution, leading to significant enhancement in response signal. Under the optimum conditions, the proposed immunosensor showed high sensitivity and selectivity with a low detection limit of 1.9 pg mL −1 and a wide linear response range of human leptin from 10 to 1000 pg mL −1 . The immunosensor was used to detect human leptin in serum, and the results were in good agreement with the data obtained by conventional ELISA method.
研究领域:
生物化学
·
医学检测
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 5.31
Zhouzhou Feng,Jiajie Yu,Jinming Kong,Tianhe Wang
In this paper, a photocatalytic system for effectively degrading azo dyes is described. The nanostructured porous Al 2 O 3 layer on glass substrate (PAOG)/silver nanoparticles (AgNPs)–Hemin composites were synthesized via photochemical reduction of Ag, self-assembly of 11-mercaptoundecanoic acid (MUA) on AgNPs and subsequently linking Hemin to MUA via coordination bond. PAOG as a nanoporous template was chemically durable, highly porous and allowed chemicals conveniently immobilized on the glass surface. PAOG had a BET surface area of 151 m 2 /g which facilitated the immobilization of AgNPs. The composite was characterized by BET surface area measurements, scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), energy dispersive X-ray analysis (EDX), Fourier transform infrared (FTIR) spectrometer and X-ray photoelectron spectroscopy (XPS). The photoreaction degradation processes were examined by UV/vis spectroscopy. Methyl orange (MO) and Acid red 18 solutions were used to evaluate the photocatalytic performance of PAOG/AgNPs–Hemin composite under visible light and UV irradiation. Compared with PAOG/Hemin, the PAOG/AgNPs–Hemin composite had enhanced photocatalytic efficiency due to higher electron transfer efficiency from AgNPs to Hemin. This novel composite with enhanced broad spectrum photocatalytic properties has promising potentials in degradation of dyes by UV and visible light utilization.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2014
·
IF 3.84
Zi-Ming Zhou,Yong Yu,Ming-Zhen Zhang,Jun Chen,Qiong-Qiong Ren,Ji-Tao Song,Bo Liu,Zhi-Ya Ma,Yuan-Di Zhao
It is important to design new ways with high specificity and sensitivity to detect lead (II). Here, ‘8–17’ DNAzyme was used as a Pb 2+ -dependent probe with high sensibility and selectivity based on chemiluminescence (CL). When Pb 2+ was present, the substrate strand was cleaved and the active hemin/G-quadruplex DNAzyme structure was formed in the presence of another sequence which contained two parts, one could be complementary with part of the substrate strand and the other was a G rich subunit. The active hemin/G-quadruplex DNAzyme structure could catalyze luminol oxidation and quantitative analysis of Pb 2+ was carried out according to the CL of luminol. Results showed that CL intensity of luminol at 452 nm had a linear relation with the concentration of Pb 2+ in the range from 5 nM to 1 μM, and the limit of detection was 0.79 nM ( S / N = 3). This method was also used to detect the contents of Pb 2+ in lake water and tobacco and the results (62.4 ± 4.0 nM and 15.2 ± 2.2 nM, respectively) were extremely closed to those of inductively coupled plasma-mass spectrometry (ICP-MS) (57.0 ± 1.4 nM and 14.5 ± 0.91 nM, respectively).
研究领域:
生物化学
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环境科学
·
分析化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Chang-Jie Gu,Fen-Ying Kong,Zhi-Dong Chen,Da-He Fan,Hai-Lin Fang,Wei Wang
A facile and effective strategy is demonstrated for the synthesis of ternary reduced graphene oxide-Hemin-Au (rGO-H-Au) nanohybrids . The nanohybrids were synthesized through a one-pot in situ reduction of GO and HAuCl 4 under alkaline conditions using GO, Hemin and HAuCl 4 as the starting materials. The synthesis process can be finished within 1 h in a solution phase, without adding any additional surfactant, stabilizing agent and toxic or harsh chemical reducing agents. The resulting nanohybrids were characterized by UV–vis spectroscopy, Raman spectroscopy , transmission electron microscopy (TEM), and so on. Electrochemical measurements showed that the rGO-H-Au nanohybrids exhibited good electrocatalytic activity for the reduction of hydrogen peroxide (H 2 O 2 ). Based on this property, a simple and highly sensitive amperometric biosensor for H 2 O 2 had been developed. The linear relationships were obtained from 0.1 µM to 40 µM and the detection limit was estimated to be 30 nM. The simple and sensitive sensing platform showed great promising applications in the pharmaceutical, clinical and industrial detection of H 2 O 2 .
研究领域:
材料科学
·
电化学
·
生物传感
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DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 106
·61759-61766
·
IF 3.708
Hongmei Chen,Xingrong Tan,Juanjuan Zhang,Qiyi Lu,Xin Ou,Yuan Ruo,Shihong Chen
In this article, a new electrochemiluminescent (ECL) biosensor based on a g-C3N4–hemin nanocomposite and hollow gold nanoparticles (HGNPs) was constructed to detect lactate. Firstly, the g-C3N4 nanosheets were prepared through ultrasonication-assisted liquid exfoliation of bulk g-C3N4, which was obtained through polymerizing melamine under 600 °C. Then, the nanocomposites of g-C3N4 nanosheets and hemin were prepared to modify a glassy carbon electrode. Subsequently, HGNPs were self-assembled onto the electrode for adsorbing lactate oxidase to achieve a lactate biosensor. Due to the excellent catalytic effect of g-C3N4–hemin and HGNPs on the luminol/H2O2 ECL system, the as-prepared biosensor exhibited a good response performance to lactate with a linear range of 1.7 × 10−8 to 5.0 × 10−4 M and a detection limit of 5.5 × 10−9 M. In addition, the prepared ECL biosensor exhibited satisfying reproducibility and stability. The g-C3N4–hemin nanocomposite might have great potential application in a luminol/H2O2 ECL system.
研究领域:
分析化学
·
生物传感技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）