MOLECULAR NEUROBIOLOGY
·
2025
·
IF 4.3
Wan Yu-hui,Cheng Zeng-jing,Fan Ling-xiang,Yang De-hong,Chen Bing-lin,Chen Xiang-xin,Zhu Qing
Subarachnoid hemorrhage (SAH) is associated with high mortality and morbidity rates. In its early stages, a substantial influx of blood into the subarachnoid space triggers excessive activation of microglia, which markedly contributes to early brain injury (EBI), a pivotal determinant of poor prognosis. Tremella fuciformis polysaccharides (TFPSs), as acidic heteropolysaccharides from the fruiting bodies of Tremella, exhibit robust anti-inflammatory characteristics and many biological properties. Nonetheless, the impact of TFPSs on EBI after SAH has yet to be reported, and the molecular mechanisms underlying these effects remain elusive. We used in vivo and in vitro models to study the effects of TFPSs on microglia post-SAH. Network pharmacology analysis was used to predict the targets of TFPSs and the pathways through which it exerts its therapeutic effects. These predictions were subsequently corroborated through flow cytometry, Western blotting, immunofluorescence staining, enzyme-linked immunosorbent assay (ELISA), and quantitative real‑time polymerase chain reaction, both in vivo and in vitro. After 24 h post-SAH, TFPS-treated mice presented improved neurological function and reduced cerebral edema. TFPSs reversed microglial activation, enhanced phagocytic ability, and reduced neuronal apoptosis. Network pharmacology identified KDR as a potential target of TFPSs, with the P38 MAPK pathway as the downstream pathway. TFPSs attenuated KDR expression, inhibited the P38 MAPK/NF-κB pathway, reduced inflammatory cytokine expression, and increased microglial phagocytic capacity post-SAH. This investigation revealed that TFPSs may ameliorate EBI after SAH, potentially via the regulation of the KDR-mediated P38 MAPK/NF-κB pathway and phagocytic function.
研究领域:
神经科学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MEDICINAL CHEMISTRY RESEARCH
·
2020
·Vol. 29
·期 8
·1341-1354
·
IF 1.783
Zhu Shi-ying,Luo Fa-zeng,Sun Ping-Hua
Gibberellic acid (GA 3 ) is a tetracyclic diterpene compound which displays interesting bioactivities. Recently, its potential use for preparing antitumor drug leads has been highlighted, and various modification methods of GA 3 have been reported. Aiming at investigating GA 3 derivatives with potential antitumor activities, ring distortion of GA 3 under different conditions was carried out, and this was followed with amidation or substitution, yielding four series of derivatives. The chemical structure of these compounds were analyzed by 1 H-NMR, 13 C-NMR, HRMS, FTIR and polarimetry, and SXRD was employed to further confirm the spatial configurations of derivatives 3c and 7d . The antitumor activities of three series of derivatives were evaluated by using MTT assay and ELISA. Results shows that, among amide derivatives, compounds with a saturated linear amide showed better activity than those with an aromatic amide. Among ester derivatives, compounds with a meta -substituted benzyl group showed better activities than those with a para -substituted benzyl group. The antitumor activity of ester derivatives might possibly be linked with the inhibition of FGFR 1 activation and KDR activation. Overall, this study discussed how the antitumor activity of GA 3 was formed, thereby assisting the future design of more effective active GA 3 derivatives.
研究领域:
有机化学
·
药理学
·
肿瘤学
✓ 已验证
DOI
高影响因子
Evidence-based Complementary and Alternative Medicine
·
2016
·
IF 1.931
Shengyan Xi,Lifeng Yue,Mengmeng Shi,Ying Peng,Yangxinzi Xu,Xinrong Wang,Qian Li,Zhijun Kang,Hanjing Li,Yanhui Wang
Chronic liver disease is one of the most common diseases that threaten human health. Effective treatment is still lacking in western medicine. Semen Persicae (Taoren) and Flos Carthami (Honghua) are known to relieve acute hepatic injury and inflammation, improve microcirculation, and reduce tissue fiber. The aim of our study is to investigate the potential mechanisms of Taoren-Honghua Herb Pair (THHP) in murine model of chronic liver disease caused by Carbon Tetrachloride (CCl4). Mice were randomly divided into seven groups: (1) blank, (2) model, (3) control (colchicine, 0.1 mg/kg), (4) THHP (5.53, 2.67, and 1.33 g/kg), and (5) Tao Hong Siwu Decoction (THSWD) (8.50 g/kg). Histological change and microvessels density were examined by microscopy. Hepatic function, serum fibrosis related factors, and hepatic vascular endothelial growth factor (VEGF) were measured with ELISA. VEGF, kinase insert domain-containing receptor (KDR), Flt-1, and Akt mRNA expression in hepatic tissue were determined with PCR. Tissues of Akt, pAkt, KDR, and Flt-1 were measured with western blotting. Data from this study showed that THHP improved hepatic function and restrained the hepatic inflammation and fibrosis. Its role in inhibiting pathological angiogenesis and hepatic fibrogenesis may be through affecting the angiogenesis-associated VEGF and its upstream and downstream signaling pathways.
研究领域:
药理学
·
肝病学
·
分子生物学
·
病理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
·
2023
·
IF 2.2
Liu Lang,Wang Suji,Zuo Junfeng,Zhang Xiaohe,Peng Xiong,Wang Kang,Chen Maohua
Rhopalosiphum padi is an important global wheat pest. The pyrethroid insecticide bifenthrin is widely used in the control R. padi. We explored the resistance potential, cross-resistance, adaptive costs, and resistance mechanism of R. padi to bifenthrin using a bifenthrin-resistant strain (Rp-BIF) established in laboratory. The Rp-BIF strain developed extremely high resistance against bifenthrin (1033.036-fold). Cross-resistance analyses showed that the Rp-BIF strain had an extremely high level of cross-resistance to deltamethrin (974.483-fold), moderate levels of cross-resistance to chlorfenapyr (34.051-fold), isoprocarb (27.415-fold), imidacloprid (14.819-fold), and thiamethoxam (11.228-fold), whereas negative cross-resistance was observed to chlorpyrifos (0.379-fold). The enzymatic activity results suggested that P450 played an important role in bifenthrin resistance. A super-kdr mutation (M918L) of voltage-gated sodium channel (VGSC) was found in the bifenthrin-resistant individuals. When compared with the susceptible strain (Rp-SS), the Rp-BIF strain was significantly inferior in multiple life table parameters, exhibiting a relative fitness of 0.69. Our toxicological and biochemical studies indicated that multiple mechanisms of resistance might be involved in the resistance trait. Our results provide insight into the bifenthrin resistance of R. padi and can contribute to improve management of bifenthrin-resistant R. padi in the field.
研究领域:
昆虫学
·
害虫管理
·
毒理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2026
·
IF 2.5
Peng Liang,Yaoxin Chen,Junhao Sheng,Cheng Zhang,Jiajun Liang,Mingzhu Li,Ming Peng,Lu Cai
Clear cell renal cell carcinoma (ccRCC) is the most common kidney cancer with limited treatment options. Sijunzi decoction (SJZ), a classic traditional Chinese medicine formula, has shown therapeutic potential but its active components and mechanisms against ccRCC remain unclear. This study integrated network pharmacology and bioinformatics analysis of TCGA data to identify kaempferol and fumarine as the core anti-ccRCC components of SJZ, potentially targeting genes including SLC6A3, OPRD1, and KDR. Subsequent in vitro validation demonstrated that fumarine specifically induces necroptosis, a form of programmed cell death, in A498 ccRCC cells, rather than apoptosis or pyroptosis. Mechanistically, fumarine triggers this process by binding to SLC6A3, OPR1 and KDR and upregulating inflammatory cytokines IL-6 and TNF, leading to the activation of the necroptotic pathway and subsequent inhibition of tumor growth. Our findings not only elucidate the key material basis and mechanism of SJZ against ccRCC but also highlight fumarine as a promising novel necroptosis-inducing agent for cancer therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）