TALANTA
·
2023
·
IF 6.1
Rui Wang,Chunlan Liu,Yong Wei,Ze Ran,Tianci Jiang,Chunbiao Liu,Chen Shi,Zhuo Ren,Xingkai Wang,Zhihai Liu,Yu Zhang
The concentration of tumor markers is low, which needs a highly sensitive, stable and fast detection method. In this paper, we proposed and demonstrated a U-shape fiber SPR biosensor sensitized by MOFs materials. The surface of the U-shape SPR sensor was modified with MOFs materials to enhance the sensitivity, and the nucleic acid aptamer was immobilized on the sensor surface because of the biocompatibility of MOFs materials. By the high specificity of the nucleic acid aptamer, the MUC1 protein was recognized and detected. The testing results indicate that the sensor has a logarithmic linear response in the MUC1 protein concentration detection range of 1 pg/ml-100 μg/ml, its sensitivity and detection limit are 5.33 nm/log(μg/ml) and 0.16 pg/ml respectively. After being sensitized by MOFs, the detection sensitivity of the sensor can be increased by 1.62 times，the LOD can be decreased by 0.75 times. The sensor has high sensitivity and specificity, which has broad application prospects in clinical detection of tumor markers.
研究领域:
生物医学工程
·
肿瘤标志物检测
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 8
·087502
·
IF 3.721
Ya Li,Lina Hou,Zhiling Liu,Wenbo Lu,Man Zhao,He Xiao,Tianjun Hu,Zhanfeng Zheng,Jianfeng Jia,Haishun Wu
Mucin 1 protein (MUC1) is a well-known tumor marker overexpressed in a variety of human malignancies, and thus its highly selective and sensitive detection is significantly desired for clinical diagnostics. Herein, a sensitive electrochemical MUC1 sensing platform has been fabricated based on copper-based metal-organic frameworks (Cu-MOFs) decorated with gold-platinum alloy nanoparticles (AuPt NPs). Besides serving as a redox probe to avoid introducing extra redox media, the electroactive Cu-MOFs function as catalysts in the hydrogen peroxide reduction, resulting in electrochemical signal amplification. Meanwhile, AuPt NPs loaded on the Cu-MOFs mimic horseradish peroxidase to promote the reduction of hydrogen peroxide, giving rise to the secondary-amplified electrochemical signal. Moreover, the target MUC1 triggered catalytic hairpin assembly (CHA) further improves the sensitivity of the developed biosensor via the target recycling amplification. The constructed sensing platform displayed a desirable dynamic range between 10 fM and 10 nM in the MUC1 detection, with a sufficiently low limit-of-detection (3.33 fM). The current strategy of utilizing hybrid nanocomposite consisting of MOFs and alloy NPs and enzyme-free CHA provides a promising platform for the detection of biomarkers in the clinical diagnosis with high selectivity and sensitivity.
研究领域:
生物医学工程
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肿瘤学
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分析化学
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产品关联
DOI
高影响因子
OPTICS EXPRESS
·
2025
·Vol. 33
·期 19
·40647-40660
·
IF 3.3
Hongtao Li,Hongtao Li,Hongtao Li,Hongtao Li,Jiaoyan Dai,Jiaoyan Dai,Jiaoyan Dai,Weisheng Wang,Weisheng Wang,Weisheng Wang,Yuxuan Liu,Yuxuan Liu,Yuxuan Liu,Lei Huang,Lei Huang,Liang Lu,Liang Lu,Liang Lu,Jialiang Lv,Jialiang Lv,Jiping Lin,Jiping Lin,Jiping Lin,Lili Liang,Lili Liang,Benli Yu,Benli Yu,Benli Yu
For efficiently diagnosing early-stage breast cancer, current existing MUC1 biomarker detection methods suffer from common issues of low sensing sensitivities. In this study, a single-core two-channel optical fiber optofluidic on-chip sensor is employed to high-sensitivity detect breast cancer biomarker MUC1 protein, where a Mach-Zehnder interferometer (MZI) waveguide and a fiber Bragg grating (FBG) inscribed into the device by using a femtosecond laser direct writing technique. To improve the device’s sensitivity, two microfluidic channels are etched using hydrofluoric (HF) solution, and Au nanobipyramids (Au NBPs) material on an optical fiber is used to excite and realize the localized surface plasmon resonance (LSPR) enhancement. Thus, the device’s refractive index (RI) sensitivity increases from 25.07 to 580.22 nm/RIU. FBG compensates the device’s temperature sensitivity, achieving at -0.139 nm/℃. On the basis of the advanced LSPR enhancement strategy, the sensor can not only specifically sense MUC1 protein with an ultra-low limit of detection (LOD) of 20.74 aM, it can also detect MUC1 protein in real serum background with a LOD of 42.07 aM. The obtained ultra-low LOD is lower than most existing state-of-the-art MUC1 detection technologies. This study paves what we believe to be a new universal way for developing advanced high-sensitivity lab-in-fiber optofluidic sensing strategy.
研究领域:
肿瘤学
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生物医学工程
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分子诊断
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光学传感
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转化医学
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纳米生物技术
·
液体活检
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）