CHEMOSPHERE
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2020
·
IF 5.778
Roujia Du,Ping Chen,Qianxin Zhang,Gang Yu
Antibiotics are widespread in the environment with notable ecological risk, for which efficient and green removal technologies are demanded. As a kind of g–C 3 N 4 –based material with remarkable photocatalytic property, OCN is an oxygen- and nitrogen-linked carbon nitride organic polymer which can be synthesized through a single-step thermal polymerization method. In this study, OCN was applied for the visible-light-driven photocatalytic degradation of a typical fluoroquinolone (FQ) antibiotics enrofloxacin (ENR). The photocatalysis process achieved over 97% ENR removal within 60 min with 0.4 mg/L OCN and 4 mg/L ENR at pH 8.2. The photocatalytic mechanism of OCN at different pH was studied for the first time. It was shown that O 2 ⋅ − , 1 O 2 and h + made contributions at neutral or basic pH and 1 O 2 contributes the most (57.6% at pH 8.2), while ⋅ OH played a role only under acidic condition with a contribution rate of 23.8% at pH 3.2. The cleavage of the piperazine ring and the quinolone ring were two main degradation pathways. The common water constituents humic acid and NO 3 − showed a dual effect, but HCO 3 − and Cl − inhibited the degradation. The effect of different water matrices was tested under natural sunlight and it was only a tiny disturbance to the degradation rates. The biotoxicity test conducted using Vibrio fischeri indicated that the toxicity of degradation products became negligible after 3 h. This study demonstrated that OCN is a promising candidate for the advanced treatment and in-situ remediation.
研究领域:
环境科学
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材料科学
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化学工程
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DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2022
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IF 6.371
Sixing Dai,Yue Wang,Lei Xiao,Gazi Hao,Yubing Hu,Guangpu Zhang,Wei Jiang
Limit caused by insufficient charge transfer between interfaces is one of the current topics in the photocatalytic degradation of pharmaceuticals and personal care products (PPCPs). Photocatalysts designed as 2D/2D/2D structures have a faster separation rate of the heterojunction interface and a better carrier transfer speed to improve their performance. In this work, 2D CuO nanosheets have been synthesized by the drip method at normal temperature. And layered MXenes are introduced as the charge transfer bridges into the system of O-doped g-C 3 N 4 nanosheets and CuO nanosheets to construct a 2D/2D/2D CuO-MXene-OCN S-scheme heterojunction. The photocatalytic performance of CuO-MXene-OCN is much higher than CuO-OCN heterojunction due to the intimate interaction between interfaces. The degradation efficiencies of different PPCPs in 60 min under visible light irradiation are 98.2 % for TC, 75.6 % for MT, 92.6 % for DCF. This unique interfacial engineering gives new insight into the construction of layered S-scheme heterojunctions with 2D/2D/2D structure for photocatalytic degradation of PPCPs.
研究领域:
环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
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IF 15.1
Qian Li,Jiahui Zhang,Jiayu Zhou,Wang Xu,Zhuowei Cheng,Piet N.L. Lens,Jianmeng Chen,Dongzhi Chen
The effective and economic treatment of chlorobenzenes has become one of the most intractable issues in the field of air pollution control. Herein, a visible-light photocatalysis coupled with biodegradation (VPCB) system was developed by integrating an innovative visible-light responsive TiO 2 /oxygenous groups decorated g-C 3 N 4 (TiO 2 /OCN) with polyurethane carriers immobilized with Ralstonia sp. XZW-1 for chlorobenzene removal. Performance tests showed that light intensity, preparation of the MICs, catalyst dosage and composition of the TiO 2 /OCN cast significant influence on chlorobenzene removal efficiency, with the degradation efficiency, mineralization and dechlorination ratios of VPCB achieving 100%, 35.5%, and 99.4% under the optimal conditions, respectively, outcompeting the sum of visible-light photocatalytic system and biodegradation protocols. Cycling tests further demonstrated the excellent stability of VPCB. Mechanism analysis found that increased reactive sites, improved visible-light harvesting, accelerated charge transfer and separation, and the resulting abundant active species jointly contributed to the enhanced photocatalytic activity of TiO 2 /OCN. Photogenerated holes were most significant active species for chlorobenzene removal, followed by OH and O 2 − radicals. The carriers could effectively protect microorganisms from light illumination and strong oxidizing species, allowing the further microbial metabolism of chlorobenzene photocatalytic oxidation intermediates with good biodegradability, not only greatly promoting the microbial growth and activity, but further enhancing chlorobenzene photocatalytic degradation. Plausible synergistic mechanism for chlorobenzene degradation between photocatalysis and biodegradation was also revealed. Furthermore, the VPCB system constructed presented wide applicability towards various pollutants, and its performance was found closely related with the hydrophobicity of pollutants. This work provided insight into the design and application of the VPCB system for the efficient and economic treatment of refractory pollutants.
研究领域:
环境科学
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微生物学
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产品关联
DOI
高影响因子
Materials Today Sustainability
·
2023
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IF 7.8
D. Yang,T. Wu,H. Gao,M. Jia,L. Ji,J. Wang,Q. Zhuang,B. Yu,L. Lu,Y. Zhang,J. Liu
The rational design of a host material with strong adsorption and excellent electrical conductivity function for sulfur cathode to suppress the shuttle effects of soluble lithium polysulfide (LiPS) is crucial to advance the lithium–sulfur (Li–S) battery for future commercial applications. In this work, we designed a three-dimensional (3D) carbon fiber aerogel with graphene-like oxygenated carbon nitrogen (OCN) and TiO 2 (OCNF–TiO 2 ) as a sulfur anchoring material for Li–S battery and explored the regulating effects of TiO 2 on the electrochemical performances. The OCNF–TiO 2 /S cathode presents an initial discharge-specific capacity of 1039 mAh/g (0.1C), a rate capacity of 690 mAh/g at 1C, a cycling performance with a capacitance retention of 72.1%, and the decay of 0.13% per cycle after 200 cycles at 0.5C.
研究领域:
材料科学
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能源存储
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS LETTERS
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2016
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IF 2.437
Huan Zhou,Shiqin Kong,Zhiwei Liu,Yan Pan,Yang Liu,Mingzhi Luo,Linhong Deng
Calcium polyphosphate (CPP) incorporated calcium alginate (CA) composite beads were prepared via adding sodium polyphosphate (STPP) contained sodium alginate (SA) solution dropwise to calcium chloride (CaCl 2 ) solution. In the process Ca 2+ ion may attach to the -COO − in SA because of electrostatic attraction. Additionally, there is an interaction of PP to Ca 2+ to generate CPP, inducing intermolecular crosslinkages between CA and CPP. The rotational rheometer showed STPP addition had no significant impacts on the rheological properties of SA solution. While a combination of SEM, AFM, FTIR and swelling behavior study demonstrated CPP was successfully formed within the gel network of CA gel beads and played an active role in restricting the movability of the alginate polymer chains. The in vitro study implied CPP can promote the expression of ALP , Col-I, OCN and OPN of osteoblasts.
研究领域:
材料科学
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生物医学工程
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细胞生物学
✓ 已验证
DOI
高影响因子
ANALYST
·
2020
·Vol. 145
·期 17
·5925-5932
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IF 3.978
Qingqing Ding,Hui Chen,Chuanhui Huang,Qiaomei Lu,Ping Tong,Wenmin Zhang,Lan Zhang
Sorbents with high surface utilization and good dispersibility are of great importance for the extraction performance of magnetic solid-phase extraction (MSPE). In this study, a fish scale-like magnetic nanomaterial (Co@Co3O4/OCN) was synthesized, which can be used as a highly efficient MSPE sorbent due to its strong magnetism, special morphology, doping of N element, numerous micro–mesopore cavities and organic functional groups (hydroxyl and carboxyl). Furthermore, a Co@Co3O4/OCN-based MSPE method for monitoring the changes in the levels of three auxins (indole-3-acetic acid, indole-3-propionic acid and 3-indole butyric acid) was successfully established. Wide linear ranges (1.0–1000.0 pg mL−1) with good correlation coefficients (R > 0.9992), low limits of detection (LODs, 0.2–4.0 pg mL−1) and satisfactory repeatability (RSD ≤5.9%, n = 3) were obtained. Using the developed method, various growth parts and different growth periods of plants under Cd stress were monitored. The results showed that auxins in various parts of plants showed differential response under Cd stress, and there was a threshold for the changes in auxin levels against Cd stress. This indicates that the developed fish scale-like Co@Co3O4/OCN nanomaterial has a good application prospect for enriching small molecular targets containing hydroxyl and carboxyl groups.
研究领域:
植物学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Journal of Stomatology Oral and Maxillofacial Surgery
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2022
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IF 2.48
Yi Sun,Jianlu Shi,Xiaoan Luo,Xuehong Xu
Objective Human periodontal ligament stem cells (hPDLSCs) refer to one kind of somatic stem cells that are capable of differentiating into multiple cell kinds and undergoing robust clonal self-renewal. This work was unearthed to elucidate the possible molecular mechanism of miR-142–3p in mediating osteogenic differentiation of hPDLSCs by targeting SGK1 . Methods The hPDLSCs were isolated, cultured, and identified. hPDLSCs were identified by immunofluorescence staining and multiple differentiation ability detection. Cell proliferation ability was assessed by CCK-8 assay. hPDLSCs were induced using osteogenic differentiation medium. ALP activity was detected by alkaline phosphatase (ALP) staining and ALP activity assay, and mineralized nodule formation was determined by alizarin red staining. The expression levels of osteogenic differentiation marker proteins ALP, RUNX2, and OCN were measured by RT-qPCR. miR-142–3p candidate targets were obtained through bioinformatics analysis. The relationship between miR-142–3p and SKG1 was verified. Results miR-142–3p in hPDLSCs after osteogenic induction was down-regulated. Elevated miR-142–3p restricted hPDLSCs proliferation, and diminished ALP activity and mineralized nodule formation, as well as the expression of ALP, RUNX2, and OCN, while miR-142–3p inhibition led to inverse results. miR-142-3p inhibited SKG1 expression. SKG1 overexpression promoted hPDLSC proliferation and osteogenic differentiation, and reversed the inhibitory function of miR-142–3p on hPDLSCs. Conclusion This study highlights that miR-142–3p represses osteogenic differentiation of hPDLSCs by reducing SGK1 expression.
研究领域:
干细胞生物学
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分子生物学
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口腔生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
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IF 7.7
Jieyi Zuo,Juan Liao,Lilin Zhu,Shuang Chen,Wenxin Wen,Ying Li,Dawei Sun,Lei Xu
Osteoporosis is one of the most prevalent age-related diseases worldwide. It is characterized by a systemic deterioration in bone strength (bone density and bone mass), leading to an increase in fragility fractures. The complex pathological environment of osteoporosis presents a significant challenge to the induction of bone regeneration under osteoporotic conditions. Therefore, the development of a system for local delivery of active substances with osteoinductive effects is of practical significance in the clinical treatment of osteoporosis. In this study, we successfully loaded the anti-osteoporotic small molecule drug zoledronate (ZOL) into calcium alginate to prepare a biologically functional hydrogel, designated as ALG-ZOL-Ca. The prepared ALG-ZOL-Ca hydrogel gels quickly, making the hydrogel easy to inject and adapt to irregularly shaped bone defects, and simultaneously exhibits good bioactivity and osteoconductivity. The RT-qPCR results suggested that this hydrogel effectively promoted the expression levels of β-catenin and Axin2, which indicating a stimulative effect on the Wnt/β-catenin pathway in vitro. Moreover, ALG-ZOL-Ca hydrogel effectively promoted the expression of the OCN and SP7 genes. Therefore, this study proposes a new functional composite hydrogel that provides a potential treatment strategy for osteoporosis.
研究领域:
药理学
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材料科学
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生物医学工程
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骨科
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2024
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IF 4.8
Jiale Mao,Houxing Lei,Pingcui Xu,Shuang Liu,Jiwang Zhou,Mingrong Mei,Nani Wang,Xiaoqin Zhang
Ethnopharmacological relevance Melastoma dodecandrum (MD), a traditional ethnomedicine, has been widely used for the treatment of fractures, osteoarthritis, and osteoporosis due to its remarkable anti-inflammatory activity. However, the specific active components responsible for its therapeutic effects on orthopedic conditions remain unidentified. Aim of the study This study aimed to screen and identify key active components in MD using a combination of cell membrane chromatography and mass spectrometry, followed by cellular validation. Materials and methods A TNF-α-induced osteoblast injury model and an osteoblast membrane chromatography screening system were established to select and identify chemical components of MD that directly act on osteoblasts. The protective effects of MD on osteoblasts were assessed by evaluating cell viability, alkaline phosphatase (ALP) activity, cell mineralization and the expression of osteogenesis-related proteins OCN, RUNX2, and the TNF-α receptor protein TNFR1. Validation of the activity of individual components was also conducted. Results MD significantly improved the viability of osteoblasts under TNF-α-induced injury, enhanced ALP activity, stimulated the expression of OCN and RUNX2 proteins, and decreased the expression of TNFR1. Cell membrane chromatography screening identified 32 chemical components, including 21 flavonoids, 6 organic acids, 2 phenylpropanoids, 2 terpenes, and 1 nucleotide. Molecular docking revealed that isovitexin could bind to the specific receptor TNFR1 on the cell membrane. Furthermore, cellular validation demonstrated that isovitexin significantly protected osteoblasts. Conclusions MD and its pharmacologically active component, isovitexin, exhibit protective effects against TNF-α-induced inflammatory injury in osteoblasts, laying a solid foundation for future drug development.
研究领域:
药理学
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民族药理学
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细胞生物学
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质谱学
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色谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC Complementary Medicine and Therapies
·
2023
·Vol. 23
·期 1
·1-11
·
IF 3.9
Cai Weiye,Sun Bin,Song Chao,Liu Fei,Wu Zhengliang,Liu Zongchao
Background In mouse, it was discovered that resveratrol (Res) enhanced osteoporosis (OP) by boosting osteogenesis. Besides, Res can also have an impact on MC3T3-E1 cells, which are crucial for the control of osteogenesis and thus increase osteogenesis. Although some articles have discovered that Res enhanced autophagy to promote the value-added differentiation of MC3T3, it is unclear exactly how this affects the process of osteogenesis in mouse. Therefore, we will show that Res encourages MC3T3-E1 proliferation and differentiation in mouse pre-osteoblasts and further investigate the autophagy-related mechanism for this impact.Methods (1) MC3T3-E1 cells were separated into blank control group and various concentrations (0.01, 0.1, 1, 10, 100µmol/L) of group in order to determine the ideal Res concentration. In the Res group, Cell Counting Kit-8 (CCK-8) was used to measure the proliferation activity of pre-osteoblasts in mice in each group after resveratrol intervention. Alkaline Phosphatase (ALP) and alizarin red staining were used to gauge the degree of osteogenic differentiation, and RT-qPCR was used to measure the expression levels of Runx2 and OCN in the osteogenic differentiation ability of the cells. (2) In the experiment, four groups were set up: the control group, 3MA group, Res group, and Res + 3MA group. To examine cell mineralization, ALP and alizarin red staining were utilized. RT-qPCR and Western blot detection of cell autophagy activity levels and osteogenic differentiation capacity in each group following intervention.Results (1) Resveratrol might increase the number of mice pre-osteoblast, with the impact being most pronounced at 10µmol/L (P < 0.05). The nodules developed substantially more often than in the blank control group, and Runx2 and OCN expressions significantly increased (P < 0.05). (2) In contrast to the Res group, after 3MA purine blocked autophagy, the Res + 3MA group’s alkaline phosphatase staining and the development of mineralized nodules were reduced. Runx2, OCN, LC3II / LC3I expression decreased, p62 expression increased (P < 0.05).Conclusion The present study partially or indirectly demonstrated that Res may, through increased autophagy, induce osteogenic differentiation of MC3T3-E1 cells.
研究领域:
药理学
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细胞生物学
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分子生物学
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骨骼研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR BIOLOGY REPORTS
·
2024
·Vol. 51
·期 1
·1-15
·
IF 2.6
Wumiti Taxi,Wang Lining,Xu Bin,Ma Yong,Zhu Yihua,Zuo Xinchen,Qian Weiqing,Chu Xudong,Sun Haitao
Background Promoting the balance between bone formation and bone resorption is the main therapeutic goal for postmenopausal osteoporosis (PMOP), and bone marrow mesenchymal stem cells (BMSCs) osteogenic differentiation plays an important regulatory role in this process. Recently, several long non-coding RNAs (lncRNAs) have been reported to play an important regulatory role in the occurrence and development of OP and participates in a variety of physiological and pathological processes. However, the role of lncRNA tissue inhibitor of metalloproteinases 3 (lncTIMP3) remains to be investigated. Methods The characteristics of BMSCs isolated from the PMOP rat model were verified by flow cytometry assay, alkaline phosphatase (ALP), alizarin red and Oil Red O staining assays. Micro-CT and HE staining assays were performed to examine histological changes of the vertebral trabeculae of the rats. RT-qPCR and western blotting assays were carried out to measure the RNA and protein expression levels. The subcellular location of lncTIMP3 was analyzed by FISH assay. The targeting relationships were verified by luciferase reporter assay and RNA pull-down assay. Results The trabecular spacing was increased in the PMOP rats, while ALP activity and the expression levels of Runx2, Col1a1 and Ocn were all markedly decreased. Among the RNA sequencing results of the clinical samples, lncTIMP3 was the most downregulated differentially expressed lncRNA, also its level was significantly reduced in the OVX rats. Knockdown of lncTIMP3 inhibited osteogenesis of BMSCs, whereas overexpression of lncTIMP3 exhibited the reverse results. Subsequently, lncTIMP3 was confirmed to be located in the cytoplasm of BMSCs, implying its potential as a competing endogenous RNA for miRNAs. Finally, the negative targeting correlations of miR-214 between lncTIMP3 and Smad4 were elucidated in vitro. Conclusion lncTIMP3 may delay the progress of PMOP by promoting the activity of BMSC, the level of osteogenic differentiation marker gene and the formation of calcium nodules by acting on the miR-214/Smad4 axis. This finding may offer valuable insights into the possible management of PMOP.
研究领域:
骨生物学
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干细胞生物学
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分子生物学
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非编码RNA
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骨质疏松症
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nutrients
·
2022
·Vol. 14
·期 9
·1940
·
IF 6.706
Kuaitian Wang,Xiao Kong,Mengdi Du,Wei Yu,Zhenhua Wang,Bo Xu,Jianrong Yang,Jingru Xu,Zhili Liu,Yongqiang Cheng,Jing Gan
DEDEQIPSHPPR, the calcium-binding peptide (CBP) identified in soy yogurt, was proven to be a potential cofactor in osteoporosis prevention in our previous study, but the mechanism was unknown. In this study, the activity of alkaline phosphatase (ALP) and osteocalcin (OCN), the regulation of RUNX2, and the expression of TβRI were investigated to elucidate the underlying mechanism. The results show that CBP upregulated ALP activity and OCN concentration and increased the expression of RUNX2 and the activation of the MAPK signaling pathway. Similarly, the expression of osteogenesis-related genes in osteoblasts also increased upon CBP treatment. Moreover, the CBP-induced enhancement of ALP activity and phosphorylation levels in the p38 pathway was inhibited by treatment with a p38 inhibitor (SB203538) and TβRI inhibitor (SB431542), respectively, suggesting that p38 and TβRI were involved in the osteogenic action. Based on the signaling pathways, the intracellular calcium concentration was significantly elevated by CBP, which was correlated with the increased behavioral functions and the relative fluorescence intensity of the bone mass. These findings suggest that CBP stimulates osteoblast differentiation and bone mineralization through the activation of RUNX2 via mechanisms related to the TβRI-p38-MAPK signaling pathways, further highlighting CBP’s important potential for treating osteoporosis.Keywords:bone remodeling;soy peptide CBP;osteoblast;differentiation;p38-MAPK;RUNX2
研究领域:
生物学
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生物化学
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药理学
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骨骼生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）