NEW JOURNAL OF CHEMISTRY
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2020
·Vol. 44
·期 17
·7091-7095
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IF 3.288
Jialu Yang,Caixia Yin,Ying Wen,Yongbin Zhang,Fangjun Huo
As an important small gas signal molecule, H2S has become an important research hotspot for both chemists and biologists. Therefore, we designed and synthesized a benzene-conjugated coumarin connected to 2,4-dinitrobenzene via an oxygen atom (P1) to detect H2S and explored its applications in biological imaging. After 40 minutes of long-time irradiation and different pH tests, we found that P1 has good photostability and chemical stability, indicating that P1 had the ability to detect H2S accurately. In addition, P1 has a fast response speed, low detection limit and low toxicity. Based on these characteristics, P1 was used to detect H2S in live cell imaging.
研究领域:
化学
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生物学
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医学成像
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产品关联
DOI
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 15
·6133-6138
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IF 1.821
Li-Qiang Yan,Yun Ma,Mei-Fang Cui,Zheng-Jian Qi
A coumarin-based “Turn-on” fluorescence chemosensor 1 containing L-histidine for Al3+ was readily synthesized and investigated, which showed high selectivity and remarkable sensitivity to aluminium(III) ions based on a photo-induced electron-transfer (PET) mechanism in aqueous solution (Tris–HCl, 0.1 mM, pH 7.2). The sensor responded rapidly to Al3+ in aqueous solutions with a 1 : 1 stoichiometry. Meanwhile, it showed excellent adaptability and also responsiveness.
研究领域:
分析化学
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生物化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2020
·
IF 3.594
Hongwei Ge,Guoqiang Liu,Ranhao Yin,Zhenli Sun,Hongxia Chen,Long Yu,Pengchen Su,Mingtai Sun,Khalid A. Alamry,Hadi M. Marwani,Suhua Wang
Formaldehyde, a volatile organic compound that is ubiquitous in gas pollutants, has an indelible hazard to the health system of humans. Development of simple and convenient ways for the visual detection of formaldehyde in air is still of importance and challenging. In this paper, we report a new type of fluorescence enhancement probe (FAP), which directly exhibited fluorescence enhancement response to formaldehyde with high sensitivity and selectivity. The FAP was synthesized through the substitution reaction between hydrazine and NBD (7-nitrobenzo-2-oxa-1, 3-diazolyl) chloride, that the hydrazine group acted as both fluorescence quencher based on the photo-induced electron transfer (PET) pathway and reaction triggering factor for selectively recognizing formaldehyde. When the FAP was incubated with a formaldehyde-containing circumstance, Schiff base compound was produced by means of the aldimine condensation reaction which inhibited the PET pathway from hydrazine group to NBD, and thus turned on the fluorescence of the FAP significantly. It is worth mentioning that the FAP showed more remarkable response to formaldehyde under acid conditions. Under the optimal experimental conditions, the FAP responded linearly toward formaldehyde in a wide range of 0.015–0.8 mg•L −1 , while the fluorescence enhancement was close to 30 times. This FAP was successfully used to detect gaseous formaldehyde in indoor air.
研究领域:
分析化学
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环境科学
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生物医学
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
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2022
·
IF 10.723
Congwei Li,Lian Duan,Xinjian Cheng
Fluorescent chitosan-based hydrogel for the selective detection and adsorption of Hg 2+ /Hg + in aqueous environment was prepared through three-step synthesis strategy. NO 2 -Boron-dipyrrolemethene (BODIPY) was prepared firstly, and then the -NO 2 group was reduced to -NH 2 group. Finally, the NH 2 -BODIPY was introduced to chitosan by Schiff base formation reaction through bi-aldehyde. Eventually, fluorescent chitosan hydrogel was obtained. The as-prepared fluorescent hydrogel probe could detect Hg 2+ /Hg + through PET mechanism with the detection limit of 0.3 μM. The recognition site which combines Hg 2+ /Hg + is C N, it is just formed in the reaction with chitosan and the amino group on BODIPY. Adsorption capacity of the fluorescent hydrogel is 121 mg·g −1 , which is almost seven times of the original chitosan. The isotherm and kinetics of Hg 2+ /Hg + removal follows Langmuir isotherm and pseudo-second order kinetics , respectively. Besides, a series of fluorescent hydrogels were prepared to compare the elasticity, hydropHilicity , fluorescence intensity and adsorption capacity.
研究领域:
材料科学
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环境科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Xiao Yang,Liangyi Zhao,Siyi Yang,Miao Tang,Huanbao Fa,Danqun Huo,Changjun Hou,Mei Yang
The detection of circulating tumor DNA (ctDNA), as a practical liquid biopsy technique, was of great significance for the study of cancer diagnosis and prognosis. However, reported methods for detection ctDNA still have some limitations, such as tedious process and high cost. In this study, CsPbBr 3 nanosheet (CsPbBr 3 NS) with high water stability was prepared by etching, and its fluorescence intensity could be stably stored for 1 year. The Ti 3 C 2 T x possessed high quenching efficiency for CsPbBr 3 NS and the HOMO-LUMO orbital study revealed that the PET mechanism was responsible for fluorescence quenching. And the Ti 3 C 2 T x showed stronger affinity towards single-stranded DNA (ssDNA), as compared with double-stranded DNA (dsDNA). The probe ssDNA could be adsorbed on the surface of Ti 3 C 2 T x through π-π stacking. After the targets were recognized by probe ssDNA to form dsDNA, its affinity with Ti 3 C 2 T x decreased and the active site of Ti 3 C 2 T x recovered, causing a high quenching efficiency on CsPbBr 3 NS. Based on this, a label-free fluorescent biosensor was designed for the sensitive detection of ctDNA ( EGFR 19 Dels for non-small cell lung cancer, NSCLC). Under the optimal experimental conditions, this biosensor exhibited a detection limit of 180 fM and a linear range of 50 pM–350 pM with amplification of magnetic beads through strand displacement reaction. In addition, this sensor was applied to the detection of ctDNA in serum samples and cells lysates. This method for ctDNA detection was expected to have great potential for biomarker detection in the field of liquid biopsy.
研究领域:
癌症诊断
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纳米技术
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生物传感器
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分子生物学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）