Food Chemistry-X
·
2025
·
IF 8.2
Lin-Meng Wang,Yi-Xue Zhang,Qing Yang,Ke Li,Wu-Chao Ma,Yan-Hong Bai
In order to improve the emulsion gel properties of PSE (pale, soft, and exudative)-like chicken meat protein isolate (PPI), effect of different addition of l -lysine (L-Lys) on the emulsifying and gel properties of PPI-sunflower oil emulsion were studied by comparing control groups (pure PPI groups (PPI), only added L-Lys (Lys) and sunflower oil (SO)). Compared with control groups, adding L-Lys to the PPI emulsion resulted in a decrease in particle size and uniform distribution, the stability of emulsion was increased, and the gel exhibited higher elastic (G′), better stability and more compact microstructure was formed. Especially when 0.2 % L-Lys was added to the emulsion system, the particle size of emulsion was reduced by 76.16 %, the emulsion gel strength was increased by 286.84 %, the centrifugal loss was reduced by 51.98 % ( P < 0.05). Therefore, L-Lys can be viewed as a viable method for enhancing the functional properties of PPI
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2016
·
IF 2.653
Zhanxian Li,Haixia Li,Caixia Shi,Mingming Yu,Liuhe Wei,Zhonghai Ni
A novel rhodamine and 8-hydroxyquinoline-based derivative was synthesized, which is shown to act as a colorimetric chemosensor for Fe 3 + in aqueous solution with high selectivity over various environmentally and biologically relevant metal ions and anions with a distinct color change from colorless to pink in very fast response time (< 1 min). Fe 3 + can be detected quantitatively in the concentration range from 6.7 to 16 μM and the detection limit (LOD) on UV–vis response of the sensor can be as low as 15 nM. The ‘ in situ ’ prepared Fe 3 + complex ( 1 ⋅ Fe ) showed high selectivity toward PPi against many common anions, and sensitivity (the LOD can be as low as 71 nM). In addition, both the chemosensor and the ‘ in situ ’ prepared Fe 3 + complex are reusable for the detection of Fe 3 + and PPi respectively.
研究领域:
分析化学
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环境科学
·
生物化学
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PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Chen Zhao,Hongmei Xu,Xiaoran Zhang,Yating Meng,Shaomin Shuang,Chuan Dong
Anions are widely found in humans and the environment. The analysis of its content and distribution shows great potential in the fields of life science, environmental science and biochemical reactions. Herein, a new anthraquinone derivative appended fluorescent probe (HBD) was developed as a reversible fluorescent probe for the detection pyrophosphate (PPi) in methanol/urotropine buffer medium (1:1, v/v, pH 4.5). The probe displayed fluorescence “turn-on” response towards Al 3+ with high specificity. Due to the cleaving of the -C N bond and subsequent creation of complex caused by coordination with Al 3+ , the chelation-enhanced fluorescence (CHEF) effect was activated, resulting in a significantly enhancement fluorescence response. Further, 1 H NMR titration and HRMS analysis revealed that the Al 3+ induced hydrolysis occurs on one side of the Schiff base and led to the formation of free primary amine compound. Moreover, the binding mechanism was also recognized density functional theory (DFT) studies. Due to the strong binding ability of PPi and Al 3+ , PPi could remove Al 3+ from the complex, causing fluorescence quenching. The specific determination of Al 3+ and PPi with the detecting limit of 31.6 nM and 56.8 nM. Due to its good biocompatibility, the sensor was successfully applied to fluorescence imaging of Al 3+ and PPi in HeLa cells and zebrafish. The results show that HBD has broad application prospects in environmental and biological analysis.
研究领域:
生命科学
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环境科学
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生物化学
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高影响因子
TETRAHEDRON LETTERS
·
2018
·
IF 2.125
Xianjiao Meng,Duanlin Cao,Zhiyong Hu,Xinghua Han,Zhichun Li,Dong Liang,Wenbing Ma
In this study, we have successfully synthesized a new coumarin based fluorescent chemosensor 1 , in which tren and quinolone are introduced as receptors for sequential recognition of Cu 2+ and PPi. The structure of chemosensor 1 was characterized by 1 H NMR, 13 C NMR and ESI-HR-MS. Sensor 1 showed an obvious “on-off” fluorescence quenching response toward Cu 2+ , and the quenching efficiency reached a maximum of 99.6% with the addition of 20 equiv. of Cu 2+ . The 1 -Cu 2+ complex showed an “off-on” fluorescence enhancement response toward PPi over many competitive anions, especially HPO 4 2− and H 2 PO 4 − . The detection limit of sensor 1 was 1.9 × 10 −6 M to Cu 2+ and 5.96 × 10 −8 M to PPi. In addition, sensor 1 showed a 1:1 binding stoichiometry to Cu 2+ and sensor 1 -Cu 2+ showed a 2: 1 binding stoichiometry to PPi in CH 3 CN/HEPES buffer medium (9:1 v/v, pH = 7.4). The stable pH range of sensor 1 to Cu 2+ and 1 -Cu 2+ to PPi was from 4 to 8.
研究领域:
分析化学
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生物化学
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DOI
高影响因子
Polymers
·
2022
·Vol. 15
·期 1
·216
·
IF 4.967
Xinxin Wang,Xinyu Zu,Ting Wang,Yanan Zhao,Yan Liang,Xiaochen Wang,Qian Chai,Yunjuan Zhang,Hongzhong Chen,Hua Wang
Lactoferrin (LF) is an iron-binding glycoprotein with various biological activities that has been extensively used in food and medical applications. Several methods for detecting LF have been reported, but they still face challenges in terms of sensitivity and simplicity of detection. To achieve an accurate and efficient detection of LF, we developed a method for the determination of LF in lactoferrin supplements using carbon dots (CDs) fluorescent probes. The N, S-doped PPI carbon dots (N, S-PPI-CDs) were prepared using a protein (peanut protein isolate) and cysteamine as precursors. The prepared N, S-PPI-CDs exhibited intense blue fluorescence and good biocompatibility, while the fluorescence intensity of the N, S-PPI-CDs showed a good linear relationship with Fe2+/Fe3+concentration (0–2 μM). The N, S-PPI-CDs exhibited a high potential ability to rapidly detect Fe2+/Fe3+within 30 s, with a limit of detection (LoD) of 0.21 μM/0.17 μM. Due to the reversible binding of LF to Fe, the N, S-PPI-CDs showed a high sensitivity and selectivity for LF, with a limit of detection (LoD) of 1.92 μg/mL. In addition, LF was quantified in real sample LF supplements and showed a fluctuation in recovery of less than 2.48%, further demonstrating the effectiveness of the fluorescent N, S-PPI-CDs sensor.Keywords:N, S-PPI-CDs;fluorescence probe;biocompatibility;Fe2+/Fe3+detection;lactoferrin supplement detection
研究领域:
分析化学
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材料科学
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生物技术
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PubMed
高影响因子
TETRAHEDRON
·
2019
·
IF 2.379
Chongyang Zhao,Jian Chen,Duanlin Cao,Jianlong Wang,Wenbing Ma
In this study, we have successfully synthesized a novel coumarin-based dendrons derivative CD and its chemical structure was characterized by 1 H NMR, 13 C NMR and ESI-HR-MS. The sensor CD showed an obvious “on-off” fluorescence quenching response toward Cu 2+ with a maximum quenching efficiency of 99.8%. The CD -Cu 2+ complex showed an “off-on” fluorescence enhancement response toward PPi over many competitive anions. The detection limit of the sensor CD was 0.29 × 10 −6 M to Cu 2+ and 2.39 × 10 −9 M to PPi. In addition, the sensor CD showed a 1:1 binding stoichiometry to Cu 2+ and the sensor CD -Cu 2+ showed a 2:1 binding stoichiometry to PPi in CH 3 CN/HEPES buffer medium (9:1 v/v, pH = 7.2). The stable pH range of sensor CD to Cu 2+ and CD -Cu 2+ to PPi was from 3 to 8.
研究领域:
化学传感器
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分析化学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Zhanxian Li,Haixia Li,Caixia Shi,Wenying Zhang,Wan Zhou,Liuhe Wei,Mingming Yu
Novel rhodamine and quinoline-functionalized nano film was prepared by copolymerization and electrospinning. Propenyl monomer of rhodamine and quinoline was synthesized and then copolymerized with methyl methacrylate via solution polymerization. This prepared copolymer was electrospun into nano film, as a solid-state sensor, which exhibits highly selective recognition of Fe 3+ ions over various environmentally and biologically relevant metal ions and anions with a distinct color change from colorless to pink in very fast response time (<1 min). The sensor can detect Fe 3+ quantitatively in concentration range of 100–2000 μM, and the calculated low detection limit (LOD) value could be 1.19 μM for Fe 3+ . As the resultant product, the Fe 3+ -contaminated nano film showed recovered color change by extra addition of pyrophosphate P 2 O 7 4− (PPi) solution. It was found that the absorption intensity at 564 nm of the Fe 3+ -contaminated nano film linearly decreases with PPi concentration from 50 to 3000 μM with LOD value of 1.75 μM. In addition, both the nano films before and after Fe 3+ -contaminating are reusable for the detection of Fe 3+ and PPi, respectively.
研究领域:
分析化学
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材料科学
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环境科学
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Hanyue Xiang,Tianran Wang,Sixian Tang,Yujie Wang,Nao Xiao
A novel hydrazone-based fluorescent probe ( E )-3-((2-(benzo[ d ]thiazol-2-yl)hydrazono)methyl)-4 H -chromen-4-one ( BTC ) has been rationally designed and synthesized. BTC can subsequently detect Ga 3+ and PPi ions through the absorption and emission off–on-off response with high specificity. Importantly, fluorescent probe BTC can well discriminate Ga 3+ from Al 3+ and In 3+ . The association constant (K) was calculated as 2.06 × 10 4 M −1 , and the limit of detection (LOD) was calculated as 4.88 × 10 -2 μM. Competitive binding studies also illustrated good results of the probe BTC towards Ga 3+ . Job's plot and HRMS results substantiated the 1:1 stoichiometry between BTC and Ga 3+ ion. The interaction binding mode of BTC with Ga 3+ was proposed by HRMS, 1 H NMR spectral titration, UV–vis absorption and fluorescence spectral measurements. The combination of the restraint of the photo-induced electron transfer (PET) process and the chelation enhanced fluorescence (CHEF) process is responsible for the fluorescence enhancement of this probe. The in situ chelated BTC -Ga 3+ could further monitor pyrophosphate ion (PPi) by demetallization process with quenching fluorescence emission. Additionally, the BTC and BTC -Ga 3+ showed good cell permeability and could detect Ga 3+ and PPi ions in onion inner epidermal cells, respectively.
研究领域:
分析化学
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生物化学
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材料科学
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PubMed
高影响因子
Frontiers in Cardiovascular Medicine
·
2024
·
IF 2.8
Yang Mengyue,Bi Wen,Zhang Zhijie
Background: The relationship between human immunodeficiency virus (HIV) infection and pulmonary arterial hypertension (PAH) has garnered significant scrutiny. Individuals with HIV infection have a higher risk of developing PAH. However, the specific mechanism of HIV-associated PAH remains unclear. Our study aims at investigating the shared biomarkers in HIV infection and PAH and predicting the potential therapeutic target for HIV-associated PAH.Methods: Data for HIV infection and PAH were downloaded from Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) analysis was performed to detect shared genes in HIV infection and PAH. Enrichment analysis was conducted to identify the function of common DEGs. Protein-protein interaction (PPI) analysis was used to detect key genes. These crucial genes were subsequently verified by RT-qPCR. Finally, candidate drugs were identified by using the Drug Signatures Database (DSigDB).Results: Nineteen common DEGs were identified in HIV infection and PAH. Enrichment analysis exhibited that the functions of these genes were mainly enriched in inflammatory responses, mainly including cellular immunity and interaction between viral proteins and cytokines. By constructing PPI networks, we identified the key gene CC-type chemokine ligand 5 (CCL5), and we verified that CCL5 was highly expressed in hypoxia induced human pulmonary artery endothelial cells (hPAECs) and human pulmonary artery smooth muscle cells (hPASMCs). In addition, we predicted 10 potential drugs targeting CCL5 by Autodock Vina.Conclusion: This study revealed that CCL5 might be a common biomarker of HIV infection and PAH and provided a new therapeutic target for HIV-associated PAH. However, further clinical validation is still indispensable.
研究领域:
免疫学
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分子生物学
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药理学
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生物信息学
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2018
·
IF 2.891
Qi Wang,Xiaoye Wen,Zhefeng Fan
In this paper, a simple Schiff base fluorescent probe was synthesized by aggregation-induced emission. The probe detected Al 3+ with an “off-on” behavior, because the molecular rotation was restricted, the molecular rigidity was increased. The stoichiometry ratio of the probe to Al 3+ was 1: 2. The detection limit of Al 3+ was 0.39 μM, which was exceedingly below the WHO standard (7.41 μM) and the acceptable Al 3+ levels of (1.85 μM) in drinking water. In view of the strong binding ability between pyrophosphate (PPi) and Al 3+ , the probe-Al 3+ detected PPi with an “on-off” behavior over other physiological relevant metal ions, anion and biomolecules investigated in this study. The detection limit of PPi was 0.75 μM. Therefore, the proposed probe is a promising fluorescent sensor that can be used to detect Al 3+ and PPi.
研究领域:
分析化学
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环境科学
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荧光传感器
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超分子化学
·
环境生理学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Xinxin Wang,Yanan Zhao,Ting Wang,Yingqiu Li,Chenying Wang,Xiangzhong Zhao,Yan Liang,Hua Wang
Herein, the effects of pretreatment methods, such as ultrasound, enzymatic hydrolysis, and their cotreatment methods on the changes in structural and functional properties of peanut protein isolate (PPI) were investigated. The cotreatment method decreased the particle size, molecular weight, surface hydrophobicity, and order extent; moreover, the hydrophobic amino acids and secondary structure of PPI also changed during the cotreatment. Simultaneously, the cotreatment method positively affected the functional properties of PPI. Notably, the solubility could be greatly increased to 88% at pH 5 via ultrasound-papain (U–P) treatment, whereas emulsifying activity was improved to 143.97 m 2 /g via ultrasound-neutrase (U–N) treatment. Moreover, foaming capacity increased to 65% via U–P treatment, showing that the cotreatment method substantially improved the functional properties of PPI. Pork meatball preparation was used to further investigate the practical application of cotreated PPI. The results indicated that pork meatballs with ultrasound-alcalase (U-A) addition (PM U-A ) and ultrasound-protamex (U–Pr) addition (PM U-Pr ) exhibited better acceptance in terms of color, tissue status, and overall acceptability compared with control meatballs, indicating that the cotreated PPI has great potential in pork meatball production and can serve as a reference for PPI commercialization.
研究领域:
食品科学
·
蛋白质化学
·
肉类加工
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DOI
高影响因子
Nanoscale
·
2018
·Vol. 10
·期 15
·7163-7170
·
IF 7.233
Jiahui Zhao,Shuang Wang,Shasha Lu,Jian Sun,Xiurong Yang
As a ubiquitous hydrolysis enzyme in phosphate metabolism, alkaline phosphatase (ALP) is a significant biomarker in laboratory research and clinic diagnosis. Herein, we report a highly water-soluble Eu(DPA)3@Lap nanohybrid material for the rapid and selective assay of PPi and ALP through a luminescence off–on recognition process. Eu(DPA)3@Lap was successfully prepared in an aqueous solution, and it exhibited strong luminescence emission, high photostability, and long lifetime. More interestingly, the strong luminescence of Eu(DPA)3@Lap can be remarkably quenched by Cu2+ due to the high impetus of coordination between the DPA ligand and Cu2+ ion. Using Cu2+ as a signal transducer, the luminescence could be recovered upon the addition of PPi ion owing to the formation of a Cu2+–PPi complex; thus, a luminescence turn-on assay for PPi ions was realized. Utilizing the ability of Cu2+ to differentiate between PPi and Pi, a convenient and straightforward luminescence assay for ALP activity was accomplished based on the specific dephosphorylation of PPi to Pi. To the best of our knowledge, this elaborate luminescence sensing system constitutes the first luminescent nanohybrid material based on a europium organic complex for ALP activity assay. Furthermore, the recognition process of PPi and ALP was completed in a convenient and facile mix-and-readout manner, and it revealed significant potential in point of care testing.
研究领域:
生物化学
·
分析化学
·
材料科学
·
医学诊断
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PubMed
高影响因子
危险品化学品经营许可证（带存储）