Angiogenesis within the wound site is a necessary prerequisite for skin tissue regeneration during the process of wound healing. Skin vessels provide adequate nutrients and soluble bioactive factors to the wounded area and remove metabolites. In this study, we prepared a double-cross-linked hydrogel based on GelMA and PVAMA to load Myeloid-derived growth factor (MYDGF). Additionally, we evaluated the healing-promoting effect of the hydrogel using a full-thickness skin injury mouse model. Compared with the control and MYDGF-free hydrogels, the MYDGF-loaded hydrogels significantly increased the percentage of wound healing and promoted granulation tissue proliferation and epidermization. In vitro experiments have shown that MYDGF may augment the migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) by upregulating the expression of phosphorylated ERK and phosphorylated P38. These findings indicate that MYDGF-loaded hydrogels promote wound healing by accelerating angiogenesis.
Xu Zhang,Ye He,Xuan Li,Chuanchuan Lin,Zhang Yuan,Liangliang Dai,Feng Ma,Yi Lv,Tony Jun Huang,Mudan Ren,Kaiyong Cai,Shuixiang He
Endoscopic submucosal dissection (ESD) is one of the most important strategies for early gastrointestinal tumor treatment. However, the ideal healing of artificial ulcers produced after ESD procedure are still hard to achieve. In a gastric ulcer environment, the inherent peristole, acid condition and excessive oxidative stress seriously affect the therapeutic outcome of traditional medication. Curcumin (Cur) is a promising anti-inflammatory drug for gastric ulcer treatment, but its long-term use is still challenging. A polymer-assisted drug delivery system in gastrointestinal disease treatment is a new direction but is still at an initial stage. Herein, we report a hybrid hydrogel consisting of catechol motif-modified methacrylated gelatin (GelMAc) and methacrylated poly (vinyl alcohol) (PVAMA). GelMAc brought significant adhesive property and biocompatibility. PVAMA could effectively provide stability and mechanical strength. Moreover, mesoporous polydopamine nanoparticles were prepared as the drug carrier to load Cur via π-π stacking and hydrogen bonding interactions, and they were encapsulated in the hybrid GelMAc/PVAMA hydrogel. The prepared sample was proved to have a unique stability on gastric tissue even bearing acid (pH 2) and oxidative stress (H 2 O 2 ) conditions. The slowly released Cur from the hydrogel was capable of inducing M2 polarization of macrophages. In an in vivo gastric ulcer model, the prepared sample remarkably improved gastric ulcer healing by curbing the pro-inflammatory response and alleviating oxidative stress. An accelerated reepithelization and angiogenesis were also observed. Thus, this study provides a promising hydrogel for the treatment of ESD-induced ulcers, which may attract more attentions to explore the deeper combination between biomaterials and gastrointestinal research.
Nanofibrous scaffolds, which are morphologically/structurally similar to native extracellular matrix, are ideal biomaterials for tissue engineering and regenerative medicine. However, the use of traditional electrospinning techniques to produce three-dimensional (3D) nanofibrous scaffolds with desired structural properties presents difficulty. To address this challenge, we prepared a novel liquid–phase-collected photoinitiated polymerised aerogel 3D scaffold (LPPI-AG) using the thermally induced (nanofiber) self-aggregation method after liquid-phase electrospinning of the hydroxyapatite-doped methacrylated polyvinyl alcohol/methacrylated gelatine solution obtained by photoinitiated polymerisation. The fabricated aerogel scaffolds had a high porosity of approximately 99.01% ± 0.40% and an interconnected network structure with pore sizes ranging from submicron to ∼300 μm. The new aerogel rapidly became flowable when exposed to a solution, and it can fill gaps and repair gap edges effectively and be loaded with nutrients and growth factors that promote bone growth for bone tissue engineering. LPPI-AG scaffolds can considerably promote osteogenic differentiation of bone marrow mesenchymal stem cells in vitro. Furthermore, in vivo studies showed that the LPPI-AG scaffold significantly promoted bone formation in a mouse model of critical-size calvarial defects.
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