Two facultatively aerobic, Gram-stain-positive, rod-shaped, endospore-forming, endophytic bacteria, designated SGZ-1009Tand SGZ-1014, were isolated from the plantPennisetumsp. Strain SGZ-1009Tgrew at 5–50 °C, pH 4.5–11.0 and tolerated up to 4.5% NaCl (w/v), while strain SGZ-1014 exhibited growth at a temperature range of 10–50 °C, pH range 4.5–11.0 and tolerated up to 4.5% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these two strains belong to the genusPaenibacillus, closely related to the reference type strain ofPaenibacillus wenxiniaeDSM 100576T(similarity of 97.0%). The genomic relatedness values for both strains, SGZ-1009Tand SGZ-1014, compared to their closest reference strainP. wenxiniaeDSM 100576T, were 81.6% for average nucleotide identity and 26.0% for digital DNA–DNA hybridization, suggesting strain SGZ-1009Trepresents a novel species. The genomic DNA G+C content of strain SGZ-1009Twas 46.4%. Both strains shared anteiso-C15:0(55.9%) as the predominant fatty acid, menaquinone-7 as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. Based on these results, strains SGZ-1009Tand SGZ-1014 represent a novel species ofPaenibacillus, for which the namePaenibacillus campisp. nov. is proposed. The type strain is SGZ-1009T(=MCCC 1K08892T=GDMCC 1.4280T=KCTC 43677T=JCM 36670T).
Phytosterols have been demonstrated to be precursors of polycyclic aromatic hydrocarbons (PAHs) formed during biomass pyrolysis. Here, a novel Paenibacillus sp. was evaluated for its ability to degrade phytosterols in tobacco waste extract (TWE). The optimal conditions for cell growth and stigmasterol (a representative of phytosterols) degradation were 37 °C, pH 7.0, 1.0 g/L yeast extract, and 6.0 g/L glucose. Paenibacillus sp. could degrade stigmasterol under high concentrations of glucose (up to 130 g/L) and tolerate wide pH (5.0–9.0) and temperature (25–42 °C) ranges. The new strain could degrade stigmasterol completely into CO 2 and H 2 O, and no intermediate steroids were detected during the degradation process. Phytosterol degradation in TWE was demonstrated by high-performance liquid chromatography–tandem mass spectrometry. Under optimal conditions (37 °C, pH 7.0, with the exponential-phase cells), the total degradation ratio of phytosterols reached 38.5% in TWE, including 45.2% of stigmasterol, 37.4% of β-sitosterol, 27.3% of campesterol, and 28.7% of cholesterol. These results showed that Paenibacillus sp. is a candidate for phytosterol degradation in TWE and other biomass and is potentially useful in reducing the PAHs generated from biomass pyrolysis.
危险品化学品经营许可证（带存储）