FISH & SHELLFISH IMMUNOLOGY
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2024
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IF 4.1
Huaxing Su,Yitong Zhang,Zhongwei He,Yucong Yang,Yuqin Ren,Wei Cao,Yufeng Liu,Jiangong Ren,Yufen Wang,Guixing Wang,Chunguang Gong,Jilun Hou
Ube3a is a member of the E3 ubiquitin ligase HECTc family, and its role has been established in neurodevelopmental disorders. However, studies on its role in Japanese flounder are scarce. Thus, in this study, the ube3a of Japanese flounder was cloned, and its role in conferring resistance against Chinook salmon bafnivirus (CSBV) was analyzed. Japanese flounder ube3a encoded a protein containing 834 amino acids. Interestingly, its homology with the Atlantic halibut was determined to be 94%. In addition, there were differential expressions of ube3a in different tissues of Japanese flounder, with the highest expression level observed in the fin, followed by the gills and skin ( P ≤ 0.05). Subcellular localization analysis revealed that Ube3a is a cytoplasmic protein. We established an in vitro CSBV infection model using Japanese flounder gill cell line (FG). After ube3a overexpression, the viral load was significantly lower than that of the control group ( P ≤ 0.05). Contrastingly, after incubation of FG cells with an E3 ubiquitin ligase inhibitor, the viral load was significantly higher than in the control group ( P ≤ 0.01). Then, the expression levels of nf-κb , traf3 , and tnf-α after incubation with an E3 ubiquitin ligase inhibitor were examined. The results demonstrated that ube3a may exerted a significant antiviral effect in Japanese flounder via the ubiquitination pathway.
研究领域:
分子生物学
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免疫学
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病毒学
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渔业科学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 4
·1037-1044
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IF 2.479
Yongxia Xu,Yiming Yin,Tao Li,Honglei Zhao,Xuepeng Li,Jianrong Li,Tong Sun
Effects of lysozyme (LYS) combined with cinnamaldehyde (CA) on quality enhancement of olive flounder ( Paralichthys olivaceus ) fillets during refrigerated storage at 4 °C for 20 days were assessed. Changes of total viable count (TVC), K -value, total volatile basic nitrogen (TVB-N), thiobarbituric acid (TBA), texture profile analysis (TPA), and trichloroacetic acid-soluble peptide (TCA-soluble peptide) in samples were determined periodically. Results demonstrated that the combination of LYS and CA treatment enhanced the antibacterial activity against S. putrefaciens and P. fluorescens , and lowered TVC values. Meanwhile, LYS combined with CA significantly retarded the increases of TBA value, TVB-N, K -value, and TCA-soluble peptide content compared to the control. Furthermore, the combined treatment also effectively maintained the texture properties of flounder fillets during the storage period. The efficiency was better than that of LYS or CA treatment alone. Thus, LYS combined with CA is promising in olive flounder shelf life extension.
研究领域:
食品科学
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水产养殖
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微生物学
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DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
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2022
·
IF 7.001
Jun Wang,Xuan Li,Peng Li,Lianxu Li,Lingchao Zhao,Shaoguo Ru,Dahai Zhang
Microplastics and polychlorinated biphenyls are ubiquitous in the marine environments. To illuminate their combined biological impacts, juvenile Japanese flounder ( Paralichthys olivaceus ) were exposed to 500 ng/L PCBs alone or 500 ng/L PCBs plus 2, 20, and 200 μg/L 10-μm porous MPs for 21 days. Compared to PCBs alone, co-exposure to PCBs and 20, 200 μg/L MPs reduced fish body length and body weight, and the concurrence of MPs aggravated PCBs-induced thyroid-disrupting effects, including significantly decreased L-thyroxine and L-triiodothyronine levels, more severe damage to the thyroid tissue and gill morphology, and disturbance on the expression of hypothalamus-pituitary-thyroid axis genes. The PCBs concentrations in the seawater were decreased dramatically with the increase of MPs concentrations, confirming that MPs absorbed PCBs from the seawater. Our results demonstrated that MPs enhanced the thyroid disruption of PCBs, suggesting that the risk of MPs and thyroid-disrupting chemicals on marine organisms should be paid more attention.
研究领域:
环境科学
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海洋生物学
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毒理学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Mei Zhao,Qingkui Cui,Xiaohan Wang,Wei Chen,Dianfeng Han,Min Song,Junkui Yang,Long Xiao,Yingjiang Xu
An increase in medium-chain chlorinated paraffins (MCCPs) in marine ecosystems caused by large-scale production is a serious threat to aquatic organisms. However, its toxicity to marine organisms, especially the molecular mechanisms, is unclear. In this study, Paralichthys olivaceus fry were exposed to 1, 10, or 100 μg/L of MCCPs for 14 days. A comprehensive approach combining biochemical parameter detection, transcriptomic, and metabolomic methods was used to investigate the toxicity mechanisms. The enzymatic activities of catalase and superoxide dismutase increased with longer exposure time, while glutathione levels increased slightly, suggesting that the redox balance was disrupted. The level of malondialdehyde was highest on day 4, indicating oxidative damage. Transcriptomic analysis revealed that there were 23 differentially expressed genes at 1 µg/L, 3628 at 10 µg/L, and 2019 at 100 µg/L. It inhibited growth and development by influencing protein synthesis, glucose metabolism and other pathways. Metabolomic analysis showed that there were 152 differentially expressed metabolites at 1 µg/L, 186 at 10 µg/L, and 540 at 100 µg/L, primarily affecting lipid metabolism. Moreover, disruptions in nucleotide metabolism and signal transduction have been revealed using transcriptomics and metabolomics. In addition, several key protein (e.g., JNK, LPC, IGF1R) and metabolites (e.g., betaine, xanthosine) could initially be used as potential markers of MCCPs exposure, but further verification is still required. This study provides new insights into the potential effects of MCCPs on marine life.
研究领域:
海洋毒理学
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环境科学
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转录组学
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代谢组学
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水生生态学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Fishes
·
2026
·Vol. 11
·期 5
·306
·
IF 2.9
Jiahui Zhang,Siyang Li,Jun Zhang,Jinming Zhang,Tianyi Li,Jianhua Li,Jun Yang,Yan Wang
Japanese flounder (Paralichthys olivaceus) is an economically important species in China’s marine fishery industry. However, due to long-term intensive fishing, its wild population has declined sharply. Artificial stock enhancement has become a core measure for restoring its resources. This study aimed to investigate the effects of different immersion durations and concentrations of SrCl2solution (10, 20, 40, 80 mg/L) on strontium (Sr) deposition in the otoliths ofP. olivaceus, and to systematically evaluate the impacts of Sr marking on the fish’s antioxidant capacity and digestive enzyme activity. The results showed that the otolith Sr/Ca ratio was positively correlated with marking concentration and duration; the optimal parameters were 40 mg/L for 4 days, with the Sr/Ca ratio returning to baseline after 30 days post-marking, and a 100% marking success rate. There were no significant differences in body length, body weight, or condition factor between the experimental groups and the control group (p> 0.05), but mortality was significantly increased in the 80 mg/L group. Digestive enzymes exhibited a dose-dependent response to Sr exposure, characterized by activation at low concentrations and inhibition at high concentrations; lipase was the most sensitive, with an inhibition threshold of 10 mg/L. Sr marking within the range of 20–40 mg/L for 4–8 days significantly activated the activity of T-AOC, CAT, GPx, and SOD (p< 0.05) and reduced MDA content, indicating that the antioxidant system was activated without causing persistent oxidative damage. In conclusion, Sr marking is a safe and efficient method for otolith marking in Paralichthys olivaceus. The recommended protocol is immersion in a 40 mg/L SrCl2solution for 4 days, followed by a 30-day recovery period in clean seawater before being used for stock enhancement evaluation. This study provides a scientific basis and technical support for assessing the effectiveness of stock enhancement inP. olivaceus.
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DOI
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 4
·246
·
IF 5.743
Ailing Yang,Yue Su,Zhenzhong Zhang,Huaidong Wang,Chong Qi,Shaoguo Ru,Jun Wang
The increasing levels of environmental estrogens are causing negative effects on water, soil, wildlife, and human beings; label-free immunosensors with high specificities and sensitivities are being developed to test estrogeneous chemicals in complex environmental conditions. For the first time, highly fluorescent graphene quantum dots (GQDs) were prepared using a visible-Fenton catalysis reaction with graphene oxide (GO) as a precursor. Different microscopy and spectroscopy techniques were employed to characterize the physical and chemical properties of the GQDs. Based on the fluorescence resonance energy transfer (FRET) between amino-functionalized GQDs conjugated with anti-lipovitellin monoclonal antibodies (Anti-Lv-mAb) and reduced graphene oxide (rGO), an ultrasensitive fluorescent “ON-OFF” label-free immunosensor for the detection of lipovitellin (Lv), a sensitive biomarker derived from Paralichthys olivaceus for environmental estrogen, has been established. The immunosensor has a wide linear test range (0.001–1500 ng/mL), a lower limit of detection (LOD, 0.9 pg/mL), excellent sensitivity (26,407.8 CPS/(ng/mL)), and high selectivity and reproducibility for Lv quantification. The results demonstrated that the visible-Fenton is a simple, mild, green, efficient, and general approach to fabricating GQDs, and the fluorescent “ON-OFF” immunosensor is an easy-to-use, time-saving, ultrasensitive, and accurate detection method for weak estrogenic activity.Keywords:graphene quantum dots;visible-Fenton reaction;fluorescence resonance energy transfer;immunosensor;environmental estrogen;lipovitellin
研究领域:
环境科学
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生物化学
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分析化学
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DOI
PubMed
高影响因子
AQUACULTURE
·
2024
·
IF 3.9
Yuqing Zhang,Wenying Zhang,Yanmin Jian,Shujing Zhang,Dongwu Liu,Kang Zheng,Xungang Tan,Feng You,Qiuxiang Pang,Shuang Jiao
Olive flounder ( Paralichthys olivaceus ) is a valuable mariculture fish that shows female-biased sexual size dimorphism (SSD), yet the molecular mechanisms responsible for this phenomenon remain poorly understood. In this study, the growth differences between females and males were compared, and comparative transcriptome analyses of brains/pituitaries, livers, muscles, and gonads from 13-month-old females and males were performed. The critical time window for SSD was from 13- to 14-month-old, with females reaching significantly larger sizes than males. Muscle cell size and plasma growth hormone (GH), insulin-like growth factor 1 and 2 (IGF1, IGF2), 11-ketotestosterone (11-KT), and 17β-estradiol (E2) levels had no significant differences between the sexes of 13- and 14-month-old flounders. Furthermore, the RNA-seq data revealed differential expression in the genes encoding the receptors for these hormones, suggesting that regulation may occur at the receptor level, potentially leading to SSD during the critical window. RNA-seq analysis identified 88, 645, 414, and 15,833 differentially expressed genes between females and males in brains/pituitaries, livers, muscles, and gonads, respectively. KEGG (Kyoto Encyclopedia of Genes and Genomes) and GSEA-based KEGG analyses revealed that some pathways associated with growth and development, including “prolactin signaling pathway” in brains/pituitaries, “cell cycle”, “oocyte meiosis”, and “DNA replication” in livers, “focal adhesion”, “ECM-receptor interaction”, “protein digestion and absorption”, “arginine and proline metabolism”, and “cysteine and methionine metabolism” in muscles, and “Hippo signaling pathway” and “JAK-STAT signaling pathway” in gonads may be involved in the establishment of sexual growth differences. Notably, females had lower steroid hormone synthesis abilities than males in brains/pituitaries, livers, and gonads, indicating the importance of sex steroid hormones in the establishment of SSD. Moreover, genes including frizzled3 ( fzd3 ) and prolactin ( prl ) in brains/pituitaries, and growth hormone receptor ( ghr ), estrogen receptor ( esr1 ), insulin-like growth factor binding protein-3 ( igfbp3 ), bone morphogenetic protein 8A-like ( bmp8a ), leptin receptor ( lepr ), and lipoprotein lipase-like ( lpl ) in livers emerge as potential candidates for the establishment of SSD. In conclusion, this study may help clarify the molecular mechanisms involved in SSD-related growth traits in flounder and provide basic data for genetic breeding of flounder.
研究领域:
海洋生物学
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基因组学
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转录组学
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内分泌学
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分子生物学
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Yingying Li,Jing Xie,Jun Mei
This study investigated the effects of static soaking (SS), atmospheric tumbling (AT), vacuum tumbling (VT), and ultrasonic-assisted vacuum tumbling (U-VT) on the quality of slightly acidic electrolyzed water-pretreated flounder fillets during superchilling storage (−1 °C). U-VT achieved the highest marinade absorption rate. However, VT demonstrated superior preservation efficacy, significantly inhibiting the growth of Pseudomonas and psychrophilic bacteria, and delaying the increase in total volatile basic nitrogen (TVB-N) and K-value. Conversely, U-VT accelerated myofibrillar protein oxidation, evidenced by the most pronounced decrease in total sulfhydryl content and increase in carbonyl content. Microbial diversity analysis indicated Pseudomonas and Clostridium became dominant during storage, with Pseudomonas weihenstephanensis identified as the specific spoilage organism. The findings reveal that while ultrasound enhances efficiency, it exacerbates oxidation and spoilage; VT optimally maintains quality by mitigating protein oxidation and modulating spoilage microbiota, providing a viable strategy for superior aquatic product preservation.
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DOI
PubMed
高影响因子
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