SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
·
IF 4.3
Gan Wu,Zhaoyang Ding,Xilin Dou,Ze Chen,Jing Xie
Histamine has been known as a momentous cause of biogenic amine poisoning. Therefore, the content of histamine in foods is strictly required to be controlled within a certain range. Here, an aptamer fluorescent sensor was developed for detection of histamine. Poly [(9, 9-di-n-octylfluorenyl-2, 7-diyl)-alt-(benzo [2,1,3] thiadia-zol-4, 8-diyl)] (PF8BT) and the styrene maleic anhydride copolymer (PSMA) were used for the preparation of PF8BT-Polymer dots (PF8BT-Pdots). PF8BT-Pdots and the cyanine3-phosphoramidite (Cy3) were linked through aptamer to achieve the ratiometric detection for histamine. PF8BT-Pdots were partly quenched by Cy3 due to the fluorescence resonance energy transfer (FRET), when the histamine molecule was recognized by aptamer on the surface of PF8BT-Pdots. A linear range (3–21 μmol/L) was obtained for histamine detection with a low limit of detection (LOD = 0.38 μmol/L). PF8BT aptamer Pdots (PF8BT-A) were used to detect histamine in simply treated aquaculture water and tuna. The cell imaging of HeLa cells presented a good biosecurity and outstanding fluorescent imaging capability of PF8BT-A. The aptamer fluorescent sensors provided a new platform for rapid and accurate detection of histamine in aquatic products and had great potential for the application in food safety and quality control.
研究领域:
食品安全
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生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Bao-Feng Zhao,Fan Wang,Jia-Chen Sun,Jin-Peng Gao,Li-Juan Zhao,Zhao-Liang Shen,Zhi-Peng Li,Yi-Xuan Wang,Yuan Wang,Dan Li,Xi-Fan Mei
Spinal cord injury (SCI) inflicts devastating consequences for health. While drugs have been employed to alleviate SCI symptoms, their complex synthesis procedures and the need for specialized drug delivery raise concerns regarding administration. Herein, we utilize a green synthesis protocol to produce polymer quantum dots (Pdots) from polyvinylpyrrolidone precursors, empowered by the catalytic prowess of copper. These Copper-catalyzed Pdots (CPdots) not only offer enhanced improved fluorescence properties but also exhibit remarkable antioxidant, anti-inflammatory, and mitochondrial function repair effects. An intriguing finding emerged from our research as CPdots were observed to activate the AMPK/PGC-1α/Sirt3 pathway-a distinctive molecular mechanism that combats mitochondrial dysfunction, and mitigates calcium overload associated with acute SCI. Furthermore, the rapid metabolism and exceptional biocompatibility of our CPdots reinforce their safety and efficacy.
研究领域:
神经科学
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材料科学
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生物医学工程
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药理学
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DOI
高影响因子
ACS Applied Polymer Materials
·
2025
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IF 4.7
Zhipeng Huang,Yuyang Wu,Chenhao Yu,Zhe Wang,Chengrong Jin,Zongjun Li,Shengyan Yin
Organic semiconductor polymers have garnered significant attention within the realm of photocatalytic hydrogen generation. Researchers have designed numerous schemes to enhance the efficiency of hydrogen generation by leveraging the tunable properties of their main chains. The donor–acceptor (D–A) strategy is a recognized approach for tuning the photoelectric properties of polymer semiconductors; however, its application within organic photocatalytic hydrogen generation demands thorough investigation. Here, we use the polyfluorene derivatives to study the effect of the D–A structure on hydrogen generation properties. After these polymers were prepared into polymer dots (Pdots) through the nanoprecipitation method, they exhibited excellent hydrogen generation properties. We found that PFO-Pdots obtained a hydrogen generation rate of 0.74 mmol g–1 h–1, and PFBT-Pdots with the D–A structure had about three times the hydrogen generation rate (2.23 mmol g–1 h–1) of PFO-Pdots, while PFVA-Pdots in the presence of the D–D structure obtained only one-half of the hydrogen generation rate (0.34 mmol g–1 h–1) of PFO-Pdots. These results indicated that the D–A structure plays a critical role in enhancing the photocatalytic hydrogen generation performance of organic semiconductor polymers. This will motivate organic polymers to take advantage of their structural tunability and enable further development in the field of photocatalytic hydrogen generation.
研究领域:
材料科学
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能源化学
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有机光电材料
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DOI
高影响因子
Polymer Chemistry
·
2014
·Vol. 5
·期 12
·3824-3830
·
IF 5.368
Yan Zhang,Yujue Chen,Xing Li,Jibo Zhang,Jinlong Chen,Bin Xu,Xueqi Fu,Wenjing Tian
Fluorescent block amphiphilic copolymers are one of the most important bioimaging materials which are highly desirable for early stage cancer diagnosis and treatment. However, sometimes the application of the fluorescent block copolymers is severely limited because of the aggregation-caused quenching (ACQ) effect. In this study, new aggregation induced emission polymer dots (AIE Pdots) were prepared through a self-assembly process by using an AIE-conjugated block copolymer containing an AIE fluorophore, 9,10-bis(4-hydroxystyryl)anthracene, hydrophobic poly(ε-caprolactone) segments, hydrophilic poly(ethylene glycol) segments and folate groups. The AIE Pdots are monodispersed in H2O with an average diameter of 15 nm, and possess strong emission and high solid state fluorescence quantum efficiency (Φ = 27.0%). Furthermore, the AIE Pdots show good stability and little toxicity to living cells and thus can be utilized for targeted HeLa cell imaging. Biological imaging investigation indicated that the folic acid functionalized AIE Pdots can be applied for targeted HeLa intracellular imaging.
研究领域:
生物医学工程
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材料科学
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细胞生物学
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DOI
高影响因子
TALANTA
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2025
·
IF 6.1
Limin Hou,Xuelian Hu,Wanyue Pang,Ruoyu Cao,Wenxiu Wang,Xiuying Liu,Yiwei Tang
The development of precise and efficient analysis technologies for acetamiprid (ACE) and carbendazim (CBZ) is of paramount importance for safeguarding the environment, ensuring food safety and protecting human health. In this study, we present a novel dual-channel fluorescent sensing system for the detection ACE and CBZ. This system integrates photoswitchable polymer dots (Pdots) and carbon dots (CDs) with aptamers and Fe 3 O 4 nanoparticles. Specifically, when irradiated with 465 nm excitation light, only the Pdots probe emits a fluorescent signal. Conversely, when irradiated with 354 nm excitation light, the Pdots probe's fluorescence is quenched, and only the CDs probe emits a signal. This innovative design eliminates interference between the fluorescent probes, thereby enhancing the accuracy and sensitivity of the detection method. Based on competitive binding between the target analytes and the complementary strands (cDNA) immobilized on magnetic Fe 3 O 4 nanoparticles with the fluorescent probes, the sensing system exhibited linear detection ranges of 1–100 ng/mL, with LODs of 0.33 ng/mL for ACE and 0.40 ng/mL for CBZ. We successfully applied this method to the analysis of ACE and CBZ in tap water, apple, and cucumber samples, achieving recoveries of 96.09 %–108.17 % and 94.59 %–109.09 %, respectively. This approach offers a highly promising tool for environmental monitoring and food safety analysis, significantly enhancing the capacity to monitor and manage potential health risks associated with these compounds.
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2019
·
IF 9.518
Jiani Wang,Shupei Zhang,Hong Dai,Hongli Zheng,Zhensheng Hong,Yanyu Lin
A delicate dual-readout immunosensor based on tetraphenylporphyrin-polymer dots (TPP-Pdots) with brilliant photoelectrochemical and photothermal performance was first successfully fabricated for the ultrasensitive detection of sialic acid (SA). Herein, TPP-Pdots with good biocompatibility , extraordinary light-harvesting ability and excellent photothermal conversion efficiency was used to capture SA antibody as dual-functional bioprobe for generating photocurrent and temperature signal. Furthermore, the large surface and morphology-mediated of rutile-TiO 2 (R–TiO 2 ) was beneficial to load amounts of TPP-Pdots for improved PEC signal and photothermal signal. Importantly, the temperature readout resulted from the variation of target concentration could be easily obtained by a universal thermometer which was time-saving and cost-saving. Under the optimized experimental conditions, the photocurrent densities and temperature changes proportionally increased with the increasing of SA concentrations from 3.5 × 10 −5 ng/mL to 35 ng/mL (R = 0.996). Impressively, the dual-readout approach proposed here not only featured with good accuracy and high sensitivity for SA detection, but also paved the way for the development of a dual-readout immunoassay based on PEC biosensor.
研究领域:
分析化学
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生物传感器
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纳米技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
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