JOURNAL OF BASIC MICROBIOLOGY
·
2016
·Vol. 56
·期 11
·1194-1202
·
IF 1.585
Xia Tang,Shunwen Dong,Wenjin Shi,Ni Gao,Lei Zuo,Heng Xu
This study focused on the bioremediation role of Pleurotus eryngii in different characteristics soils contaminated with nickel (Ni) and fluoranthene. The results of bioremediation experiments showed that fluoranthene had a positive effect on the growth of P. eryngii , whereas Ni exerted a negative influence. The concentration of fluoranthene significantly decreased in inoculated soil accounting for 86.39–91.95% of initial concentration in soils and 71.46–81.76% in non-inoculated soils, which showed that the dissipation of fluoranthene was enhanced by mushroom inoculating. The highest removal rates of fluoranthene in sandy loam, loamy clay, and sandy soils reached to 87.81, 86.39, and 91.95%, respectively, which demonstrated that P. eryngii was more suitable for the bioremediation of sandy soil contaminated with fluoranthene. In addition, the presence of Ni tended to decrease the dissipation of fluoranthene in inoculated soil. Higher ligninolytic enzymes activities were detected in inoculated soils, resulting in the enhanced dissipation of fluoranthene in inoculated soils. Furthermore, P. eryngii had the ability to uptake Ni (4.88–39.53 mg kg −1 ) in co-contamination soil. In conclusion, the inoculating of P. eryngii was effective in remediating of Ni-fluoranthene co-contaminated soils.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Menghan Yuan,Xinyue Huang,Shuang Tian,Shixuan Ma,Yuxing Guo,Mingxuan Tao
The selenized modification of purified Pleurotus eryngii polysaccharides (PPEP) were carried out in a nitric acid-sodium selenite system, and the preliminary structural characterization of PPEP and selenized PPEP (613.87 μg/g of selenium) (SPPEP) were carried out by advanced instrumentation techniques. It was found that the selenization modification did not seriously affect the structure of the PPEP. However, the SPPEP exhibited a lower molecular weight, more glycosidic bonds, reduced crystallinity, and improved thermal stability. Through in vivo animal experiments, the study found that PPEP and SPPEP were non-toxic to macrophages. Furthermore, they markedly increased the thymus and spleen indices of immunocompromised mice and improved the histomorphology of these organs. Moreover, PPEP and SPPEP enhanced the mice’s immune ability by markedly increasing the level of immune factor secretion, bone marrow hematopoietic function, and macrophage phagocytic capacity. In addition, the immune activity of SPPEP was better than PPEP’s activity.
研究领域:
生物技术
·
免疫学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
Food & Function
·
2024
·
IF 5.1
Xinyi Li,Qi Tao,Qiuhui Hu,Ning Ma,Gaoxing Ma
Pleurotus eryngii protein is considered high quality protein based on its rich and comprehensive essential amino acids, and has also been proved displaying multiple significant functional characterizations of which vary with its fabrication process. We aimed to investigate the variation differences of P. eryngii protein extracted by alkaline extraction and acid precipitation (AA), cellulase complex alkaline extraction and acid precipitation (CAA), ultrasound assisted alkaline extraction and acid precipitation (UAA), and salt dissolution method (S) from the perspective of gastrointestinal digestion and fecal fermentation consequences. Protein hydrolysis and structural analysis was performed on after in vitro gastrointestinal digestion, and found that AA had the highest hydrolysis degree while CAA had the lowest. The results of fluorescence chromatography and infrared chromatography indicated that the reasons for the digestion difference might be the unfolding degrees of protein tertiary structure and the polysaccharide which was the highly contained component in crude protein and had the opportunity to prevent protein hydrolysis. Metagenomic analysis suggested that compared with other groups, AA had excellent biological functions including regulating obesity and insulin related microbiota. This study could provide new theoretical basis for the P. eryngii protein as a novel type of nutrient and functional component, and contribute to the development of constructing a diversified emerging food protein supply system.
研究领域:
食品科学
·
营养学
·
微生物学
·
蛋白质化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Gaoxing Ma,Qian Xu,Hengjun Du,Benard Muinde Kimatu,Anxiang Su,Wenjian Yang,Qiuhui Hu,Hang Xiao
Pleurotus eryngii is a valuable new edible mushroom variety cultivated on a large scale in China. The polysaccharides found in this mushroom are strong bioactive. This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota. The results showed that the molecular weight of PEP remained unchanged after simulated digestion, and the overall structure of PEP was not destroyed, indicating that PEP was not decomposed during digestion. However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures. Meanwhile, PEP regulated the composition of intestinal flora, and the relative abundance of Firmicutes increased significantly. These suggests that PEP can be used as a functional food to promote intestinal health and prevent disease.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2020
·Vol. 55
·期 6
·2492-2508
·
IF 2.773
Yuanyuan Zhao,Xuefeng Chen,Wei Jia,Guoli Gong,Yanni Zhao,Guoliang Li,Jie Zhou,Xiaona Li,Yu Zhao,Wenjin Ma
Summary Pleurotus eryngii polysaccharide (PEP) was obtained using hot water extraction and ethanol precipitation. Three purified polysaccharide fractions (namely PEP1-A, PEP2-A and PEP3-A) were obtained from PEP using DEAE-cellulose-52 chromatography and a gel permeation Sephadex G-100 column. Firstly, this paper examined the characterisation of PEP1-A, PEP2-A and PEP3-A. The corresponding molecular weights were 5.378 × 10 5 , 9.506 × 10 6 and 4.975 × 10 5 Da, respectively. PEP1-A, PEP2-A and PEP3-A had similar monosaccharide compositions. PEP1-A was β-configuration, and PEP2-A and PEP3-A were α-configuration. PEP1-A, PEP2-A and PEP3-A had pyran-type rings, (1 → 3) glucose and (1 → 6) galactose linkages. Secondly, PEP, PEP1-A, PEP2-A and PEP3-A possessed antioxidant activities, and PEP was best. Therefore, only PEP was used to study its anti-fatigue activity in vivo . The result proved that PEP had anti-fatigue activity. PEP could be used as a valuable natural food supplement for preventing anti-fatigue or functional food.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yan Fang,Yukai Lin,Yanjing Ou,Linyu Wang,Jiang Chen,Caixia Sun,Yunxiang Wen,Haiqing Liu
Chitin , the second most abundant polysaccharide on earth, possesses unique characteristics, including biosafety, biodegradability , and procoagulant activity, making it an attractive material for hemostasis . However, the conventional bottom-up construction of chitin-based materials is intricate and time-consuming. In this study, we have developed a top-down strategy to prepare a 3D porous chitin-based hemostatic sponge with exceptional hemostatic properties and antibacterial activity , directly from the spongy Pleurotus eryngii . The top-down method involves deproteinization, in situ quaternization, and tannin acid crosslinking. The obtained sponge has an interconnected microporous structure with high porosity (89.7 ± 3.2 %), endowing it with high water absorption (2047 ± 105 %) and rapid water-triggered shape-memory behavior (< 2 s). The sponge exhibits superior blood coagulant activity and outperforms standard medical gauze, gelatin sponge, and chitosan sponge in both topical artery and non-compressive liver puncture wound. In addition, the sponge exhibited significant antibacterial activity against both gram-positive Staphylococcus aureus and gram-negative Escherichia coli . In summary, this study provides a straightforward and practical approach for constructing an antibacterial and hemostatic chitin sponge that could be a valuable option for treating bleeding wounds.
研究领域:
生物材料学
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医学
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Caixia Sun,Pengpeng Yue,Runlan Chen,Shuangquan Wu,Qifa Ye,Yunxiang Weng,Haiqing Liu,Yan Fang
Efficient hemostasis is a great challenge for treating the inaccessible hemorrhage wounds. A novel shape-memory chitin-glucan hemostatic sponge (ATC-Sponge) is constructed via sequentially in-situ removal of protein and glucan from Pleurotus eryngii fruiting body, TEMPO oxidation and Ca 2+ crosslinking. The sponge displays interconnected microporous structure with high water absorption and robust mechanical properties. The sponge at dry state shows rapid blood-triggered shape-memory, allowing easy insertion into the puncture wound in a compressed fixed-shape and the subsequent quick volume expansion to conform wound shape to stop bleeding. Compared with standard medical gauze and gelatin sponge, ATC-Sponge demonstrates superior hemostatic performance in the rat femoral artery and non-compressive liver puncture injury models. Additionally, ATC-Sponge can effectively accelerate wound healing. This multi-functional shape-memory ATC-Sponge shows high potential in controlling the bleeding of inaccessible traumas.
研究领域:
生物材料学
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医学工程
·
创伤治疗
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Jiang Jikang,Li Wenxiang,Yu Shuping
To investigate the effects of inoculating Leuconostoc mesenteroides or Lactiplantibacillus planetarium on the changes in metabolites in Pleurotus eryngii Jiaosu, a fermented plant extract rich in nutrients and bioactive compounds, the physicochemical and metabolite features of the treated Jiaosu were compared to those of spontaneously-fermented Jiaosu. The physicochemical features of Jiaosu fermented with Leuconostoc mesenteroides or Lactiplantibacillus planetarium were superior to spontaneously-fermented Jiaosu. An Partial Least Squares Discrimination Analysis (PLS-DA) score plot showed a clear difference in metabolites between spontaneous fermentation group (SFG) and inoculated Lactiplantibacillus planetarium group (LPG). Metabolites were remarkably different between LPG and SFG on the first day of fermentation. The differential metabolites included sugars, acids and alcohols. This study highlights the applicability of GC-MS based metabolomics as a tool to monitor Jiaosu fermentation.
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Xianrong Huang,Jingming Zhu,Mengyang Zhang,Yu Fang,Qisheng Liu,Fang Zhang,Xiang Lu
With the depletion of fossil fuels and rising environmental issues, there’s a pressing need for solar energy storage materials that boost solar utilization and decrease dependence on nonrenewables. Traditional organic phase change materials face limitations in solar energy storage applications due to their proneness to leakage and low light absorption coefficients. Herein, a novel strategy that involves the in situ polymerization of pyrrole on Pleurotus eryngii (Pe) biomass, addressing these challenges by improving light absorption while preserving the high porosity of Pe. The resulting phase change composites (PCCs) achieved an encapsulation rate of 89.7%, a light absorption rate of 96.1% within the wavelength range of 300–2500 nm, and an impressive photothermal conversion efficiency of 91.2% under simulated solar irradiation at an intensity of 5 suns. In summary, our innovative strategy effectively boosts the light absorption coefficient of Pe biomass while preserving its high porosity, providing a feasible solution for the large-scale and expedited manufacturing of solar photothermal energy materials. This work highlights the promise of biomass in driving renewable energy technology forward and supports the pursuit of sustainable energy solutions.
研究领域:
材料科学
·
能源工程
·
高分子化学
·
可再生能源
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
DESALINATION
·
2026
·
IF 9.9
Bo Li,Xianling Hu,Haoyue Yang,Jiyue Wang,Hanting Mao,Yuanshuo Li,Ke Wu,Zihao Li,Duanju Niu
Solar-driven interfacial evaporation (SDIE) has emerged as a sustainable solution for global freshwater scarcity. However, rational structural design is often hindered by the lack of direct correlation between local evaporation kinetics and macroscopic geometry. Herein, a simulation-guided subtractive manufacturing strategy is proposed utilizing natural Pleurotus eryngii as a processable functional substrate. Distinct from traditional thermodynamic analyses, COMSOL Multiphysics was employed to directly visualize the theoretical evaporation rate distribution, revealing that a pyramidal array topography maximizes the active evaporative area by mitigating vapor stagnation on inclined surfaces. Guided by these insights, the biomass coated with graphite nanopowder (GNP) and polyvinyl alcohol (PVA) was rationally tailored via mechanical carving. Microscopically, the intrinsic hierarchical porous network reduced the equivalent enthalpy of vaporization to ∼1447 kJ kg −1 . Macroscopically, the optimized evaporator with a pyramidal array achieved a remarkable evaporation rate of 3.84 kg m −2 h −1 and an energy conversion efficiency of 154% in pure water under 1.0 sun irradiation (1 kW m −2 ), representing a 14.3% enhancement over its flat-top counterpart and validating the simulation predictions. The structural advantage proved robust in a 3.5 wt% sodium chloride (NaCl) solution under 1.0 sun irradiation, maintaining a high evaporation rate of 3.52 kg m −2 h −1 . Furthermore, outdoor experiments demonstrated a peak evaporation rate of 10.42 kg m −2 h −1 under natural sunlight conditions, alongside versatile broad-spectrum ion rejection and wastewater purification capabilities. This study establishes a paradigm where theoretical evaporation kinetics informs macroscopic structural design, offering a scalable, low-cost, and high-efficiency solution for sustainable water remediation.
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Tao Wang,Xin Wu,Xuyuan Li,Wei Feng,Ren Wang,Kai Huang
Fungi proteins represent a potential alternative to animal proteins, which are regarded as a prospective source of edible proteins due to more sustainable and efficient cultivation of fungi. Nevertheless, the extraction of fungi proteins typically involves alkali extraction combined with acid precipitation (AEAP), yielding considerable amounts of salt that causes aggregation and inferior functional properties of proteins. In this study, we put forward a novel extraction method by direct filtration of Na + and OH − from the alkaline extraction solutions using ceramic membranes. This method enabled the continuous, efficient and cost-effective production of Pleurotus eryngii protein (PEP). The PEP prepared by this technology featured a uniform distribution of nanoparticles (∼129.5 nm) with higher water dispersity, colloidal stability and surface active properties as compared to that prepared by the AEAP method. Moreover, the microstructures of PEP can be tailored with various nanoparticle sizes and size distribution profiles, allowing for the adjustment of long-term stabilities and surface activities. Our study renovated the production technology of fungi proteins to enhance their functionalities as well as the application prospects in the future food industry.
研究领域:
食品科学
·
蛋白质工程
·
可持续农业
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 10
·1443
·
IF 5.561
Hongbo Li,Suya Xie,Shangqiao Cao,Liangbin Hu,Dan Xu,Jiayi Zhang,Haizhen Mo,Zhenbin Liu
Edible fungi play an important role in material and energy cycling. This study explored the role ofPleurotus eryngiimycelia in the transformation of potato high-calorie starch to low-calorie β-glucan. First, the 3D printing performance of the potato medium was optimized. After inoculating the fermentation broth ofPleurotus eryngiiin 3D printing, we studied the microstructure and material composition of the product. Along with the increase in 3D printing filling ratio, the starch content of the culture product decreased from 84.18% to 60.35%, while the starch content in the solid medium prepared using the mold was 67.74%. The change in β-glucan content in cultured products was opposite to that of starch, and the content of the culture product increased from 12.57% to 24.31%, while the β-glucan content in the solid medium prepared using the mold was 22.17%. The amino acid composition and content of the 3D printing culture system and solid culture products prepared using the mold were similar. The 3D printing culture system promoted the bioconversion efficiency of mycelia. It also showed high application potential ofPleurotus eryngiimycelia for the preparation of low-calorie food.Keywords:bioconversion;Pleurotus eryngiimycelia;starch;3D printing;β-glucan
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）