FOOD CHEMISTRY
·
2023
·
IF 8.8
Shijin Zhou,Liang Guo,Xinheng Shi,Lele Ma,Huaixia Yang,Mingsan Miao
An electrochemical sensor based on environmentally friendly eRAFT polymerization was developed for the detection of aflatoxin B 1 (AFB 1 ) in food and herbal medicine. Two biological probes, aptamer (Ap) and antibody (Ab), were used to specifically recognize AFB 1 , and a large number of ferrocene polymers were grafted on the electrode surface by eRAFT polymerization, which greatly improved the specificity and sensitivity of the sensor. The detection limit of AFB 1 was 37.34 fg/mL. In addition, the recovery rate was 95.69% to 107.65% and the RSD was 0.84% to 4.92% by detecting 9 spiked samples. The delighted reliability of this method was verified by HPLC-FL.
研究领域:
分析化学
·
食品科学
·
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Soil Ecology Letters
·
2024
·Vol. 6
·期 3
·1-11
·
IF 4.5
Liao Hongkai,Zheng Chunli,Li Juan,Long Jian,Li Yaying
Phyla Proteobacteria and Bacteroidetes dominated in the rhizosphere of tomatoes under RSD amendment.
Stochastic processes dominated in bacterial community assembly for biochar and CF amendments.
It is important to monitor and manage soil conditions before planting after RSD amendment.
Reductive soil disinfestation (RSD) is an important tool for sustainable agricultural productivity. However, the differences in soil bacterial communities and their community assembly processes among RSD and other treatment strategies (e.g., biochar and chemical fumigation) are still subject to open questions. In this study, soils subjected to various treatments–untreated control (CK), chemical soil fumigation with CaCN 2 (CF), 1% biochar (1%B), 3% biochar (3%B), and reductive soil disinfestation (RSD) are investigated. Soil samples were collected, incubated, and then used for growth of tomato plants. The Sloan neutral community model indicates that stochastic processes dominate in bacterial community assembly for both biochar and CF amendments. In contrast, this work shows that RSD treatment can have a strong impact on soil bacterial community composition. The relative abundance of Firmicutes increased during unplanted soil incubation, whereas Proteobacteria and Bacteroidetes dominated in the rhizosphere after planting of tomatoes. Normalized stochasticity ratio reveals that deterministic selection played an important role in the bacterial assembly under RSD amendment. We found that RSD amendment yielded lower biomass than that for other treatments after 28 days of tomato growth. Our results suggest that although RSD treatment has great potential to rebuild soil bacterial ecology by shaping bacterial communities and their assembly processes, it is important to monitor and manage soil conditions (e.g., soil nutrients or physical properties) before planting to ensure plant productivity.
研究领域:
土壤微生物学
·
植物-微生物互作
·
农业可持续性
·
微生物生态学
·
土壤科学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 44
·34737-34743
·
IF 3.84
Yufeng Shan,Yong Yang,Yanqin Cao,Hao Yin,Nguyen Viet Long,Zhengren Huang
Recently, surface-enhanced Raman scattering (SERS) has been the subject of great interest because of its ultrasensitive, rapid and nondestructive analysis, detection, and imaging. Highly sensitive, stable and recyclable substrates are of great importance in fulfilling the practical potential of the technique in various fields, such as trace analysis, diagnosis and criminal investigation. In this work, we introduce wafer-scale Ag-modified hydrogenated black TiO2 nanowires as active SERS substrate by hydrogenation. The Ag/H–TiO2 NWs substrates deliver a relative enhancement factor of up to ∼102 compared with the substrate before hydrogenation and a detection limitation improved by at least three orders of magnitude compared with R6G detection. Moreover, hydrogenated TiO2/Ag also exhibits outstanding stability, and is recyclable by self-cleaning under UV irradiation. The excellent SERS performance is ascribed to the abundant surface electronic states induced by the hydrogenated amorphous layers and trivalent titanium, and more photogenerated charge carriers may contribute to the chemical and electromagnetic enhancement. The relative standard deviation (RSD) value for Raman signals less than 0.15 confirms the reproducibility of the hydrogenated composite SERS substrate. Therefore, the hybrid SERS substrates have great advantages in self-cleaning, reproducibility and stability, making them promising candidates for sensitive and recyclable SERS detection.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
Materials Chemistry Frontiers
·
2023
·Vol. 7
·期 6
·1100-1109
·
IF 7
Suyang Li,Junjie Chen,Wanbing Xu,Biao Sun,Jiechen Wu,Qiang Chen,Pei Liang
Bimetallic gold core–silver shell (Au@Ag) surface-enhanced Raman scattering tags draw broad interest in the fields of biological and environmental analyses. Herein, an efficient hybrid microfluidic chip was designed to prepare uniform Au@Ag core–shell nanoparticles, and DTNB was used as the internal standard tag molecule to prepare Au@DTNB@Ag for SERS detection. Homogeneous core–shell nanoparticles with a particle size of 90 nm were prepared by mixing a silver precursor and a gold core in a microfluidic chip. The relative standard deviation (RSD) of the particle size distribution was close to 10%, and the detection limit of 4-MBA was as low as 10-10 M. In order to solve the influence of SERS signal fluctuation, a uniform Au@DTNB@Ag core–molecule–shell structure was synthesized in a microfluidic chip, and the characteristic peak of the analyte was corrected by the relative intensity of the DTNB characteristic peak (1335 cm−1). The experimental results showed that the SERS detection was achieved with high reproducibility, and the SERS peak intensity had a good linear correlation with the concentration. The homogeneous SERS substrate prepared using a microfluidic chip has potential for sensitive and reliable detection of environmental chemical contaminants.
研究领域:
纳米技术
·
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2017
·
IF 3.255
Yang Huang,Ying Feng,Guangyun Tang,Minyi Li,Tingting Zhang,Marianne Fillet,Jacques Crommen,Zhengjin Jiang
Flavonoids from plants always show a wide range of biological activities. In the present study, a rapid and highly efficient supercritical fluid chromatography (SFC) method was developed for the separation of 12 flavonoids. After careful optimization, the 12 flavonoids were baseline separated on a ZORBAX RX-SIL column using gradient elution. A 0.1% phosphoric acid solution in methanol was found to be the most suitable polar mobile phase component for the separation of flavonoids. From the viewpoint of retention and resolution, a backpressure of 200 bar and a temperature of 40 °C were shown to give the best results. Compared with a previously developed reverse phase liquid chromatography method, the SFC method could provide flavonoid separations that were about three times faster, while maintaining good peak shape and comparable peak efficiency. This SFC method was validated and applied to the analysis of five flavonoids (kaempferol, luteolin, quercetin, luteoloside, buddleoside) present in Chrysanthemum morifolium Ramat. from different cultivars (Chuju, Gongju, Hangju, Boju). The results indicated a good repeatability and sensitivity for the quantification of the five analytes with RSDs for overall precision lower than 3%. The limits of detection ranged from 0.73 to 2.34 μg/mL, while the limits of quantification were between 2.19 and 5.86 μg/mL. The method showed that SFC could be employed as a useful tool for the quality assessment of Traditional Chinese medicines (TCMs) containing flavonoids as active components.
研究领域:
分析化学
·
生药学
·
药物分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Optical Materials Express
·
2021
·Vol. 11
·期 4
·1234-1246
·
IF 3.442
Chen Junfan,Zhang Cong,Zhang Jie,Zhu Yong
Two types of Ag grating arrays as surface-enhanced Raman scattering (SERS) were fabricated using the monolayer self-assembled polystyrene (PS) as a template, using the silver film and monocrystalline silicon wafer as the substrates, imprinting different thicknesses of silver (50 nm, 75 nm, 100 nm, 125 nm and 150 nm) on the template, then removing PS. Rhodamine 6G (R6G) was used as a probe to characterize the performance of Raman enhancement. Experimental results showed that two structures can obtain a Raman enhancement factor of more than 107 at arbitrary deposition thicknesses. The insensitivity of two arrays induced by incident polarization and the difference in near-field and far-field simulation were also compared. These novel SERS substrates can achieve considerable uniformity, and the relative standard deviation (RSD) of the characteristic peak calculated at 1650 cm−1 were about 9.2% and 9.5%, respectively.
研究领域:
材料科学
·
光学
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2021
·Vol. 15
·期 6
·4849-4857
·
IF 2.431
Xu Yi,Kutsanedzie Felix Y. H.,Ali Shujat,Wang Pingyue,Li Chunyan,Ouyang Qin,Li Huanhuan,Chen Quansheng
Lead ion (Pb 2+ ) accumulation in food consumed by humans and animals possess health threats and effects. The development of a senor for its monitoring at its minutest accumulation in edible materials becomes an antidote to safeguarding human lives. A fabricated fluorescence-based sensor made of carboxyl modified upconversion nanoparticles (UCNPs), gold nanorods (GNRs), and cysteamine (Cys) were used as energy donor, receptor and a bridge respectively for the rapid sensing of Pb 2+ in Matcha. The UCNPs-Cys-GNRs sensor showed weak fluorescence signals due to the FRET, and was inhibited in the presence of Pb 2+ to form a complex with Cys, and the consequent recovery of fluorescence signals. Under optimized conditions, a good linear relationship was established between the relative fluorescence intensity and Pb 2+ concentrations from 1 to 100 μM with a low detection limit (LOD) of 0.5 μM. The developed sensor was applied to detect Pb 2+ in Matcha samples with recoveries within 93.99—102.16% and relative standard deviations (RSD) < 8%. Satisfactory results (p > 0.05) were obtained upon validation using a standard inductively coupled plasma mass spectrometry (ICP-MS).
研究领域:
分析化学
·
环境科学
·
食品科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xia Yan,Hang Zhao,Hongyan Song,Jun Ma,Xiaofeng Shi
Surface-enhanced Raman scattering (SERS) has great potential for the detection of marine pollutants, but it is still restricted in ultra-trace and quantitative analysis. Here, a strategy for the detection of polycyclic aromatic hydrocarbons (PAHs) was proposed based on Au nanoscale convex polyhedrons (Au NCPs) coated with high-energy facets and embedded with 4-ATP as a probe molecule. Au NCPs acted as SERS substrates and led to limits of detection (LODs) for six common PAHs that reached 0.01 nM. Using internal calibration, the relative standard deviations (RSD) of the spectral stability and reproducibility were as low as 3.36% and 5.11%, respectively. The maximum mean relative errors (AREs) of the predicted and true values were 6.28%. The results indicate that the resulting Au NCPs improved the ultra-trace and quantitative detection of SERS, thus suggesting that the Au NCPs have practical application value in SERS.
研究领域:
环境科学
·
分析化学
·
纳米技术
·
材料科学
✓ 已验证
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Wenfei Zhao,Wen Wang,Yixuan Wu,Wenhui Guo,Wenzhuo Ren,Shiyu Meng,Shaojun Yun,Cuiping Feng
In this study, a spent mushroom substrate (SMS) from Lentinus edodes cultivation was used to prepare biochar (BC) and magnetic biochar (MBC) at high temperatures. The magnetic field strength of MBC was proved via VSM analysis. The results of the nitrogen adsorption‒desorption isotherms of BC and MBC showed that MBC exhibited stronger adsorption, and SEM was performed to compare the microstructures of BC and MBC. TEM was performed to compare the distributions of C, O, and Fe. The FT-IR and XRD results revealed changes in the structure and the formation of new substances after magnetization; that is, MBC was more conducive to the adsorption and recovery of pesticides. Moreover, a method for the detection of tetramethrin, beta-cypermethrin, and fenvalerate in water samples (tap water, lake water, and river water) was established, and this method combined the use of MBC as adsorbent and high-performance liquid chromatography. Moreover, great linearity of the method was obtained in the range of 0.5–2.5 μg/mL; the recoveries in real samples were in the range of 70.20–73.32%, and the RSDs were in the range of 0.22–1.09%. The method is simple and effective, is suitable for the detection of pyrethroids in water, and has certain application potential.
研究领域:
环境科学
·
分析化学
·
材料科学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2023
·
IF 5
Wenjing Qian,Jingming Zhou,Yumei Chen,Hongliang Liu,Peiyang Ding,Yankai Liu,Chao Liang,Xifang Zhu,Ying Zhang,Enping Liu,Aiping Wang,Gaiping Zhang
Virginiamycin (VIR), a feed additive, is used to promote pig and poultry growth. However, it is hazardous to human health. This work described a label-free electrochemical immunosensor based on silver nanoparticles-reduced graphene oxide (AgNPs-rGO) nanocomposites and staphylococcal protein A (SPA) for the first time to directly detect the residual marker VIR M1. Good catalytic currents for oxygen reduction reaction were apparently obtained after the modification of nanocomposites on gold electrode. Nanocomposites were characterized using UV–Vis, X-ray diffraction (XRD) patterns, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). SPA was targeted to immobilize VIR M1 monoclonal antibody (mAb) by binding to Fc region of antibody. The proposed immunosensor showed a wide linear range from 0.25 ng mL −1 to 100 ng mL −1 , providing detection limit (LOD) of 0.18 ng mL −1 of VIR M1. Recovery rates ranged from 92.27% to 98.84%, and relative standard deviation (RSD) was not above 6.6%, indicating the immunosensor could detect VIR M1 in actual samples with high accuracy. The sensor showed good selectivity, reproducibility and stability and could be considered as a potential tool for detection of VIR M1 in feed and animal derived food.
研究领域:
分析化学
·
电化学
·
生物传感器
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Hui Zhangsun,Qinzhi Wang,Zhihao Xu,Jiao Wang,Xiaoshuai Wang,Yijian Zhao,Hui Zhang,Shuang Zhao,Lihua Li,Zhenyu Li,Li Wang
Enhancing catalytic activity is the key to develop electrochemical sensors for monitoring of neonicotinoid such as imidacloprid (IMI), thiamethoxam (THX) and dinotefuran (DNF). Herein, a MOF-derived octahedral NiCu nanoporous carbon composite with abundant N -doped (N/ [email protected] ) was synthesized, which was exploited as electrochemical sensor for neonicotinoid determination. Integrating the synergistic effect of NiCu nanoalloy and the hierarchical porous carbon structure, the N/ [email protected] /GCE significantly promoted the diffusion between electrolyte and active site. Meanwhile, the introduction of polyvinylpyrrolidone (PVP) improved the hydrophilicity and dispersibility of N/ [email protected] , endowing superior electrocatalytic performance for IMI, THX and DNF determination, with linear ranges of 0.5–60, 1–60 and 0.5–60 μM, respectively, and detection limits of 0.017, 0.007 and 0.001 μM. Additionally, the sensor can determine IMI, THX and DNF in apples, tomatoes and potatoes with the recovery rate of 92.1–103.4% and RSD ≤ 4.7%. This study has explored a novel modified electrode material for construction of neonicotinoid sensors.
研究领域:
材料科学
·
电化学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）