JOURNAL OF APPLIED MICROBIOLOGY
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2019
·Vol. 127
·期 6
·1716-1726
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IF 2.683
Cheng R.,Wang L.,Li J.,Fu R.,Wang S.,Zhang J.
AimsTo purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS‐secreting performance and investigate its anti‐inflammatory activity using lipopolysaccharide (LPS)‐induced macrophage cells in an acute liver injury mouse model.Methods and ResultsTwelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source. Agrobacterium sp. ZCC3656 exhibited the highest EPS yield (21·1 g l−1) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays. The EPS, designated Riclin, was purified by deproteinization using the Sevag method. The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan‐like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8. : 1·0 : 0·9 : 1·1 and has an molecular weight of approximately 2·5 × 106 Da. Riclin inhibited TNF‐α, IL‐1β and IL‐6 expression in LPS‐stimulated RAW 264.7 macrophage cells in a dose‐dependent manner. In addition, Riclin pretreatment increased the survival rate of D‐Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF‐α, IL‐1β and IL‐6.ConclusionsAgrobacterium sp. ZCC3656 is a highly stable EPS‐producing strain. The EPS Riclin from ZCC3656 is a succinoglycan‐type polysaccharide that is noncytotoxic and exhibits remarkable anti‐inflammatory effects in vivo and in vitro.Significance and Impact of the StudySuccinoglycans are well known for good rheological properties and their physiological interactions with plants. However, their potential activity towards mammals has received little attention. Our study revealed that the succinoglycan Riclin exhibited excellent anti‐inflammatory activities and could be considered as a promising reagent in anti‐inflammatory treatment.
研究领域:
微生物学
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免疫学
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药理学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Yaqiong Miao,Zenghui Chen,Jinrun Zhang,Nan Li,Zhenxuan Wei,Yan Zhang,Xinyi Wu,Junhao Liu,Qin Gao,Xiaqing Sun,Qi Sun,Jianfa Zhang
Food freshness monitoring is vital to ensure food safety. Recently, packaging materials incorporating pH-sensitive films have been employed to monitor the freshness of food products in real time. The film-forming matrix of the pH-sensitive film is essential to maintain the desired physicochemical functions of the packaging. Conventional film-forming matrices, such as polyvinyl alcohol (PVA), have drawbacks of low water resistance, poor mechanical properties, and weak antioxidant ability. In this study, we successfully synthesise PVA/riclin (P/R) biodegradable polymer films to overcome these limitations. The films feature riclin, an agrobacterium -derived exopolysaccharide . The uniformly dispersed riclin conferred outstanding antioxidant activity to the PVA film and significantly improved its tensile strength and barrier properties by forming hydrogen bonds . Purple sweet potato anthocyanin (PSPA) was used as a pH indicator. The intelligent film with added PSPA provided robust surveillance of volatile ammonia and changed its color within 30 s in the pH range of 2–12. This multifunctional colorimetric film also engendered discernible color changes when the quality of shrimp deteriorated, demonstrating its great potential as an intelligent packaging material to monitor food freshness.
研究领域:
食品科学
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材料科学
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生物化学
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DOI
PubMed
高影响因子
COLLOID AND POLYMER SCIENCE
·
2026
·
IF 2.3
Zhang Hongmei,Wang Pengge,Yang Yunxia,Zhu Daliang,Wang Yanqing
Succinoglycan riclin (RIC), a natural polysaccharide with diverse biological activities, was employed as a drug carrier to construct a novel nanoplatform. Polysaccharide-Pt(IV) conjugates were synthesized via esterification between the hydroxyl groups of RIC and a Pt(IV) prodrug, which was subsequently loaded with resveratrol (RES) to form the RES@RIC-Pt(IV) nanoplatform. The successful synthesis and physical properties of the complex were confirmed through multiple characterization techniques. Pt(IV) was significantly bonded to the polysaccharide chain. The drug loading capacities for platinum and RES were quantified using ICP-OES and UV–Vis spectrophotometry, respectively. The percentage of Pt content in RIC-Pt (IV) conjugates can be calculated as 11.71%. In vitro cytotoxicity assays revealed that the RES@RIC-Pt(IV) platform exhibited significantly enhanced toxicity against HepG2 and LO2 cancer cell lines compared to individual small-molecule drugs. These results demonstrate that this multi-drug delivery system achieves a potent synergistic anticancer effect, highlighting its potential for improved cancer therapy. Graphical abstract
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DOI
高影响因子
Gels
·
2025
·Vol. 11
·期 8
·672
·
IF 5.3
Yunxia Yang,Hongmei Zhang,Xueqing Zhang,Shuyan Shen,Baojuan Wu,Dexin Peng,Jie Yin,Yanqing Wang
Bacterial infections cause serious problems associated with wound treatment and serious complications, leading to serious threats to the global public. Bacterial resistance was mainly attributed to the formation of biofilms and their protective properties. Hydrogels suitable for irregular surfaces with effective antibacterial activity have attracted extensive attention as potential materials. In this study, a succinoglycan-riclin-zinc-phthalocyanine-based composite (RL-Zc) hydrogel was synthesized through an amine reaction within an hour. The hydrogel was characterized via FT-IR, SEM, and rheology analysis, exhibiting an elastic solid gel state stably. The hydrogel showed large inhibition circles onE. colias well asS. aureusunder near-infrared irradiation (NIR). RL-Zc hydrogel exhibited positively charged surfaces and possessed a superior penetrability toward bacterial biofilm. Furthermore, RL-Zc hydrogel generated abundant single oxygen and mild heat rapidly, resulting in disrupted bacterial biofilm as well as amplified antibacterial effectiveness. A metabolomics analysis confirmed that RL-Zc hydrogel induced a metabolic disorder in bacteria, which resulted from phospholipid metabolism and oxidative stress metabolism related to biofilm disruption. Hence, this study provided a potential phototherapy for biofilm-induced bacterial resistance.
研究领域:
生物材料
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抗菌治疗
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光动力治疗
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伤口愈合
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生物膜破坏
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高分子化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2024
·
IF 7
Yunxia Yang,Yonggan Zhuo,Chuangda Zhu,Hongmei Zhang,Yanqing Wang
Traditional ice is usually employed to preserve food freshness and extend shelf life. However, ice cannot bear repeated freeze − thaw cycles during the transportation and retailing process, resulting in microbial cross-contamination and spoilage of foods. Herein, succinoglycan riclin was oxidated (RO) and crosslinked with gelatin (Ge), the Ge-RO cryogels were prepared via Schiff base reaction and three freeze − thaw cycles. The Ge-RO cryogels showed improved storage modulus (G′) and thermal stability compared with pure gelatin hydrogel. The polymer framework of Ge-RO gels exhibited stable properties against ice crystals destructions during nine freeze − thaw treatments. During the storage and repeated freeze − thaw treatments of shrimps, Ge-RO cryogels exhibited a remarkable preservation effect on shrimps, and their freshness was evaluated using an electronic nose technique equipped with ten sensors. The results demonstrated that the shrimp muscle preserved in ice generated off-odors and resulted in high sensor responses. The sensor responses were reduced sharply of shrimps preserved in cryogels. Moreover, 1 H NMR-based metabolomics analysis revealed that shrimps in Ge-RO cryogels group reversed the metabolic perturbations compared with the traditional ice group, the metabolic pathways were related to energy metabolism, nucleotide metabolism, and amino acid metabolism, which provide new clues to the freshness of shrimps. Furthermore, RO exhibited superior antimicrobial activity against E. coli and S. aureus microorganisms. Thus, the crosslinked cryogels are potentially applicable to food preservation, offering sustainable and reusable solutions against traditional ice.
研究领域:
食品科学
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材料科学
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生物化学
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DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2024
·
IF 5.5
Weiling Lu,Xianjin Wang,Changchang Kong,Shijunyin Chen,Chengtao Hu,Jianfa Zhang
Hydrogels composed of natural polysaccharides have been widely used as filling materials, with a growing interest in medical cosmetology and skin care. However, conventional commercial dermal fillers still have limitations, particularly in terms of mechanical performance and durability in vivo. In this study, a novel injectable and implantable hydrogel with adjustable characteristics was prepared from succinoglycan riclin by introducing PEG diglycidyl ether as a cross-linker. FTIR spectra confirmed the cross-linking reaction. The riclin hydrogels exhibited shear-thinning behavior, excellent mechanical properties, and cytocompatibility through in vitro experiments. Furthermore, when compared with subcutaneous injection of a commercial hyaluronic acid hydrogel, the riclin hydrogels showed enhanced persistence and biocompatibility in Balb/c mice after 16 weeks. These results demonstrate the great potential of the riclin-based hydrogel as an alternative to conventional commercial soft tissue fillers.
研究领域:
生物材料
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组织工程
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医学美容
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）