Therapeutic Advances in Gastroenterology
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2021
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IF 4.409
Fenghua Xu,Yi Cheng,Guangcong Ruan,Liqin Fan,Yuting Tian,Zhifeng Xiao,Dongfeng Chen,Yanling Wei
Background: The community of gut microbes is a key factor controlling the intestinal barrier that communicates with the nervous system through the gut-brain axis. Based on our clinical data showing that populations of Roseburia intestinalis are dramatically decreased in the gut of patients with ulcerative colitis, we studied the efficacy of a strain belonging to this species in the context of colitis and stress using animal models. Methods: Dextran sulfate sodium was used to induce colitis in rats, which then underwent an enema with R. intestinalis as a treatment. The disease activity index, fecal changes and body weight of rats were recorded to evaluate colitis, while histological and immunohistochemical analyses were carried out to examine colon function, and 16S rRNA sequencing was performed to evaluate the gut microbiota change. Behavioral assays and immunohistochemical staining of brain were performed to assess the effect of R. intestinalis on the gut-brain axis. Results: Colitis-related symptoms in rats were significantly relieved after R. intestinalis enema, and the stool traits and colon length of rats were significantly recovered after treatment. The gut epithelial integrity and intestinal barrier were restored in treated rats, as evidenced by the higher expression of Zo-1 in colon tissues, accompanied by the restoration of gut microbiota. Meanwhile, depressive-like behaviors of rats were reduced after treatment, and laboratory experiments on neuronal cells also showed that IL-6, IL-7 and 5-HT were downregulated by R. intestinalis treatment in both serum and brain tissue, while Iba-1 expression was reduced in treated rats. Conclusions: The administration of R. intestinalis contributes to restoration of the gut microbiota, promoting colon repair and the recovery of gastrointestinal function. These alterations are accompanied by the relief of depressive-like behaviors through a process modulated by the neuronal network and the regulation of inflammation by the gut-brain axis.
研究领域:
胃肠病学
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微生物学
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神经科学
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产品关联
DOI
PubMed
高影响因子
ACS Nano
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2025
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IF 16
Huijie Hu,Xiaomin Su,Qing Dong,Shuai Yuan,Shaokang Ding,Juxiong Liu,Bin Xu,Liqun Tu,Xuanting Liu,Yu Cao,Xinyue Wang,Guiying Yang,Wenjin Guo,Shoupeng Fu,Daiyong Chao
Inflammatory bowel disease (IBD) is marked by severe colonic inflammation and oxidative stress-induced tissue damage, often accompanied by gut microbiota dysbiosis. Probiotics have recently been recognized as promising therapeutic agents for IBD. Herein, we examined the modulatory effects of orally administered metal–organic framework (MOF) nanozyme MOF-818 on gut microbiota in a mouse model of IBD. Oral treatment with MOF-818 significantly alleviated IBD symptoms, reduced intestinal oxidative damage, and favorably altered the gut microbiota composition. Notably, MOF-818 selectively enhanced the proliferation of Roseburia intestinalis (R. intestinalis). Further analyses showed that MOF-818 significantly upregulated bacterial division-related genes (ftsZ, ftsE) in R. intestinalis while genes associated with ATP synthesis (atpA, atpD) were also highly expressed, boosting energy production to support rapid cell division and proliferation. These findings underscore the biofunctional role of MOF-818 as an active modulator of the gut microflora rather than a passive therapeutic agent, which highlights its significant synergistic potential with probiotics and lays a theoretical foundation for the future application of MOF-based materials in combination with probiotics.
研究领域:
纳米医学
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微生物学
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胃肠病学
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材料科学
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免疫学
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2021
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IF 6.475
Siya Wu,Jihui Zuo,Yang Cheng,Ya Zhang,Zhongshan Zhang,Mingjiang Wu,Yue Yang,Haibin Tong
Type 2 diabetes mellitus (T2DM) is considered a rapidly growing chronic disease that threatens human health worldwide. Extracts of various seaweeds have been shown to have anti-diabetic activity. Sargarsum fusiforme, an edible brown seaweed, has been shown to possess anti-inflammatory, anti-diabetic and anti-obesity activities. In this study, we investigated the beneficial effect of an ethanol extract of S. fusiforme (EE) on type 2 diabetes in mice induced with high-fat diet (HFD) and streptozotocin (STZ). Administering EE to the diabetic mice significantly reduced food intake, water intake and fasting blood glucose (FBG), while improving glucose tolerance, lipid profile and ameliorating hepatic oxidative stress. Furthermore, these animals also exhibited significantly diminished epididymal fat deposition, as well as less pathological changes in the heart and liver tissues, while displaying some highly enriched benign gut bacteria (e.g., Intestinimonas , Oscillibacter , Lachnoclostridium, unidentified_Lachnospiraceae , Roseburia and Anaerotruncus ) and a lower abundance of bacteria associated with diabetes or other metabolic diseases (e.g., Enterorhabdus and Romboutsia ). Metabolomic analysis revealed reduced levels of branched-chain amino acids (BCAA), such as l -valine and l -isoleucine, aromatic amino acids (AAA), such as l -tyrosine and l -phenylalanine, and increased levels of 4-hydroxyphenylacetic acid (4-HPA) in the gut content, suggesting that EE may impact T2DM through modulation of these compounds in the gut of the animals. Taken together, the results implied that S. fusiforme may contain valuable active components other than polysaccharides that have potential benefit in alleviating T2DM.
研究领域:
药理学
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营养学
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糖尿病研究
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微生物学
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代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）